CN102021215B - Method for extracting gelatin peptides - Google Patents
Method for extracting gelatin peptides Download PDFInfo
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- CN102021215B CN102021215B CN 201010540517 CN201010540517A CN102021215B CN 102021215 B CN102021215 B CN 102021215B CN 201010540517 CN201010540517 CN 201010540517 CN 201010540517 A CN201010540517 A CN 201010540517A CN 102021215 B CN102021215 B CN 102021215B
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Abstract
The invention discloses a method for extracting gelatin peptides. In the method, gelatin is taken as a main raw material; and the method comprises the following steps of: performing heating and boiling with water, enzymolysis with protamex, external cutting with flavourzyme, enzyme inactivation, decoloring and membrane separation, and finally drying obtained concentrated solution to obtain the gelatin peptides. The method provided by the invention ensures a short production cycle, low cost, no toxic and harmful substances and high safety of the obtained product, and can be widely applied in the fields of health care products, medicaments and the like.
Description
Technical field
The inventive method relates to the deep process technology field of donkey-hide gelatin, is specifically related to a kind of process for extracting of donkey-hide gelatin peptide, be a kind of be raw material with the donkey-hide gelatin, thereby utilize compound bio-enzyme enzymolysis donkey-hide gelatin to obtain the method for donkey-hide gelatin peptide.
Background technology
Donkey-hide gelatin is that dry skin or the fresh hide of equine species donkey is through decocting, concentrate the solid gums of processing.Nature and flavor are sweet, flat, return lung liver kidney channel, and function is enriched blood, hemostasis, nourshing Yin and drynsessmoistening prescription, cures mainly deficiency of blood disease, and other can be used for the assisting therapy of tumour.The donkey-hide gelatin master contains collagen protein; But digesting and assimilating of collagen protein needs proteolytic enzyme to participate in degraded; Indication crowd is then common weakness of the spleen and the stomach or gastrointestinal reaction is arranged, and take the burden that donkey-hide gelatin not only can cause patient's Digestive tract, and drug effect can not be fully played.
And adopt modern enzyme incision technology that donkey-hide gelatin is handled the small molecules donkey-hide gelatin peptide that obtains, can effectively overcome the problems referred to above, for human health brings glad tidings.The donkey-hide gelatin peptide has following characteristic: 1, the donkey-hide gelatin peptide has tangible blood tonification effect to iron-deficiency anaemia.It has the effect of rising red corpuscle and oxyphorase, can promote hemopoietic function; Ability leukocyte increasing, thrombocyte and the raising oxygen content of blood.2, the donkey-hide gelatin Toplink increases serum calcium levels, regulates the balance of calcium in the human body, improves anoxia, fatigue resistance.3, the donkey-hide gelatin peptide has effects such as hemostasis, antifatigue, shock, radioprotective, anti-flesh impotence, cold resistant, raising body immunity, enriching yin and nourishing kidney, strengthening the tendons and bones and inducing diuresis to remove edema.In addition, it can expand capillary blood vessel, enlarges Q volume of blood, reduces blood viscosity and reduces the effect of vessel wall permeability.
At present, the method for extracting the donkey-hide gelatin peptide mainly contains alkali liquor extraction method and biologic enzymolysis method, and the former has used a large amount of alkaline purifications in leaching process, not only environment was polluted but also there is potential safety hazard in human body, has also destroyed proteinic composition.The preparation method of one Chinese patent application donkey-hide gelatin oligopeptide, what use among the publication number CN 101294186A is that stomach en-and trypsinase are hydrolyzed, and carries out ultrafiltration with the ultrafiltration post then; Then use the macroporous adsorbent resin separation and purification, decompression, cryoconcentration; The dry finished product that gets, this method complex operation step is not suitable for suitability for industrialized production; And concentration operation destroys the article phase of product easily, does not also use membrane separation technique, has influenced the quality of product.The present invention is on the basis of combinative enzyme hydrolysis, and then it is circumscribed to adopt flavor protease to carry out orientation, in the total protein of the product that obtains, molecular weight at the daltonian donkey-hide gelatin peptide of 500-3000 more than 97%.
Summary of the invention
To the deficiency that exists in the prior art, the object of the present invention is to provide a kind of process for extracting of donkey-hide gelatin peptide, be raw material with the donkey-hide gelatin, need not to use alkaline purification, utilize complex enzyme zymohydrolysis, and then, extract the donkey-hide gelatin peptide through membrane separation technique desalination and concentrated.The present invention is easy and simple to handle, and is environmentally friendly, is fit to very much suitability for industrialized production.
In order to realize above-mentioned technical purpose, the present invention takes following technical measures:
A kind of process for extracting of donkey-hide gelatin peptide, its step is following:
1, be raw material with authentic Donga donkey-hide gelatin piece (protein 60-80wt%), the 6-8 zero(ppm) water doubly that adds raw material weight stirs, and is warming up to 100 ℃ of boiling 6-8h;
2, be cooled to 50-60 ℃, adjust pH is 7-8, adds Sumizyme MP and trypsinase in the ratio of the 1-2.5% of raw material donkey-hide gelatin weight and 0.5-1% respectively then, in the reaction process with sodium hydroxide solution maintenance system pH at 7-8, constant temperature stirring enzymolysis 6-10h; The 1-2% that presses raw material donkey-hide gelatin weight again adds flavor protease, stirs enzymolysis 5-8h, and keeping the pH value of reaction system in the enzymolysis process is 7-8; The enzymolysis solution that obtains is warming up to 90-100 ℃, keeps the 10-20min enzyme that goes out;
3, with the centrifugal after-filtration of enzymolysis solution, remove proteolytic enzyme, the 2-2.5% that presses raw material donkey-hide gelatin weight in the supernatant adds powdered active carbon; 55 ℃ of whip attachment 1h decolourings; And then use the membrane sepn means to separate, and concentrate, liquid concentrator is drying to obtain donkey-hide gelatin peptide dry product.
The enzyme activity of the Sumizyme MP that uses in the inventive method, trypsinase and flavor protease is respectively: 25U/mg, 35U/mg and 30U/mg.
Compared with prior art, advantage of the inventive method and beneficial effect are following:
1, avoided effectively that chemical means is handled the environmental pollution that brought and to human injury's problem;
2, the purity of the donkey-hide gelatin peptide product produced of the inventive method is high, and molecular weight reaches more than 97% at the daltonian peptide content of 500-3000 in its gross protein, and molecular weight >=3000 are daltonian in 3%;
3, the molecular weight of the donkey-hide gelatin peptide produced of the inventive method is little, and human body absorbs easily, and specific absorption reaches more than 90%;
4, the inventive method has adopted activated carbon decolorizing, and products obtained therefrom is white uniform powder, has guaranteed the natural whiteness of product;
5, the inventive method with short production cycle, easy and simple to handle, cost is low, safe without toxic side effect, can be widely used in fields such as multi-function health-care article, medicine.
Embodiment
Through concrete embodiment the inventive method is done further detailed description below.
Embodiment 1:
A kind of process for extracting of donkey-hide gelatin peptide, its step is following:
1, takes by weighing authentic Donga donkey-hide gelatin piece (protein 60-80wt%) 1kg, add zero(ppm) water 7kg then, after stirring, be warming up to 100 ℃ of boiling 8h;
2, be cooled to 55 ℃; Food grade hydroxide flake sodium solution with 0.1M is transferred pH to 7-8; Add 25g Sumizyme MP and 10g trypsinase then, stir enzymolysis 10h down at 55 ℃, the pH value stabilization that keeps reaction system with above-mentioned 0.1M sodium hydroxide solution in the enzymolysis process is at 7-8; Add the 10g flavor protease again, 55 ℃ are stirred enzymolysis 8h, and the sodium hydroxide solution with above-mentioned 0.1M in the enzymolysis process keeps pH value of reaction system at 7-8; Be warming up to 95 ℃ then, the insulation 15min enzyme that goes out;
3, enzymolysis solution is filtered, get supernatant; Add the 25g powdered active carbon in the supernatant, 55 ℃ of whip attachment 1h decolourings, filtering gac; The filtrating via hole diameter is that the nanofiltration membrane separation system of 1nm separates, and desalination also concentrates; Liquid concentrator is spray-dried, obtains the Powdered donkey-hide gelatin peptide of 415g white (adopt activated carbon decolorizing among the present invention, the color and luster of products obtained therefrom is a white, is superior to the faint yellow of national Specification), and the examining report of products obtained therefrom is seen table 1.
The examining report of table 1 embodiment 1 products obtained therefrom
Embodiment 2:
A kind of process for extracting of donkey-hide gelatin peptide, its step is following:
1, takes by weighing authentic Donga donkey-hide gelatin piece (protein 60-80wt%) 2kg, add zero(ppm) water 14kg then, after stirring, be warming up to 100 ℃ of boiling 6h;
2, be cooled to 50 ℃; Solution with the food grade flaky sodium hydrate of 0.1M is transferred pH to 7-8; Add 20g Sumizyme MP and 10g trypsinase then, stir down enzymolysis 8h at 50 ℃, in the enzymolysis process with the pH value stabilization of the sodium hydroxide solution maintenance reaction system of above-mentioned 0.1M at 7-8; Add the 30g flavor protease again, 50 ℃ are stirred enzymolysis 7h, and the sodium hydroxide solution with above-mentioned 0.1M in the enzymolysis process keeps pH value of reaction system at 7-8; Be warming up to 100 ℃ then, the insulation 10min enzyme that goes out;
3, enzymolysis solution is filtered, get supernatant; Add the 45g powdered active carbon in the supernatant, 55 ℃ of whip attachment 1h decolourings, filtering gac; The filtrating via hole diameter is that the nanofiltration membrane separation system of 1nm separates, and desalination also concentrates; Liquid concentrator is spray-dried, obtains 835g white powder donkey-hide gelatin peptide, and the examining report of products obtained therefrom is seen table 2.
The examining report of table 2 embodiment 2 products obtained therefroms
Embodiment 3:
A kind of process for extracting of donkey-hide gelatin peptide, its step is following:
1, takes by weighing authentic Donga donkey-hide gelatin piece (protein 60-80wt%) 10kg, add zero(ppm) water 80kg then, after stirring, be warming up to 100 ℃ of boiling 7h;
2, be cooled to 55 ℃; Solution with the food grade flaky sodium hydrate of 0.1M is transferred pH to 7-8; Add 200g Sumizyme MP and 100g trypsinase then, stir down enzymolysis 7h at 55 ℃, in the enzymolysis process with the pH value stabilization of the sodium hydroxide solution maintenance reaction system of above-mentioned 0.1M at 7-8; The flavor protease that adds 200g again, 55 ℃ are stirred enzymolysis 5h, and the sodium hydroxide solution with above-mentioned 0.1M in the enzymolysis process keeps pH value of reaction system at 7-8; Be warming up to 90 ℃ then, the insulation 20min enzyme that goes out;
3, enzymolysis solution is filtered, get supernatant; Add the 250g powdered active carbon in the supernatant, 55 ℃ of whip attachment 1h decolourings, filtering gac; The filtrating via hole diameter is that the nanofiltration membrane separation system of 1nm separates, and desalination also concentrates; Liquid concentrator is spray-dried, obtains 4.16kg white powder donkey-hide gelatin peptide, and the examining report of products obtained therefrom is seen table 3.
The examining report of table 3 embodiment 3 products obtained therefroms
Embodiment 4:
A kind of process for extracting of donkey-hide gelatin peptide, its step is following:
1, takes by weighing authentic Donga donkey-hide gelatin piece (protein 60-80wt%) 100kg, add zero(ppm) water 600kg then, after stirring, be warming up to 100 ℃ of boiling 6h;
2, be cooled to 60 ℃; Solution with the food grade flaky sodium hydrate of 0.1M is transferred pH to 7-8; Add 1.5kg Sumizyme MP and 0.75kg trypsinase then, stir down enzymolysis 6h at 60 ℃, in the enzymolysis process with the pH value stabilization of the sodium hydroxide solution maintenance reaction system of above-mentioned 0.1M at 7-8; The flavor protease that adds 1.8kg again, 60 ℃ are stirred enzymolysis 6h, and the sodium hydroxide solution with above-mentioned 0.1M in the enzymolysis process keeps pH value of reaction system at 7-8; Be warming up to 95 ℃ then, the insulation 15min enzyme that goes out;
3, enzymolysis solution is filtered, get supernatant; Add the 2kg powdered active carbon in the supernatant, 55 ℃ of whip attachment 1h decolourings, filtering gac; The filtrating via hole diameter is that the nanofiltration membrane separation system of 1nm separates, and desalination also concentrates; Liquid concentrator is spray-dried, obtains 42.5kg white powder donkey-hide gelatin peptide, and the examining report of products obtained therefrom is seen table 4.
Table 4 embodiment 4 products obtained therefrom examining reports
Claims (1)
1. the process for extracting of a donkey-hide gelatin peptide, its step is following:
1), be raw material with the Donga donkey-hide gelatin, the 6-8 zero(ppm) water doubly that adds raw material weight stirs, and is warming up to 100 ℃ of boiling 6-8h;
2) after, boiling finishes; Be cooled to 50-60 ℃, adjust pH is 7-8, adds the Sumizyme MP of 25U/mg and the trypsinase of 35U/mg in the 1-2.5% of raw material donkey-hide gelatin weight and the ratio of 0.5-1% respectively then; Constant temperature stirs enzymolysis 6-10h, keeps system pH at 7-8 in the reaction process; The 1-2% that presses raw material donkey-hide gelatin weight again adds the flavor protease of 30U/mg, stirs enzymolysis 5-8h, and keeping the pH value of reaction system in the enzymolysis process is 7-8; The enzymolysis solution that obtains is warming up to 90-100 ℃, the insulation 10-20min enzyme that goes out;
3), with the centrifugal after-filtration of enzymolysis solution, remove proteolytic enzyme, the 2-2.5% that presses raw material donkey-hide gelatin weight in the supernatant adds the powdered active carbon decolouring, and then uses the membrane sepn means to separate, and concentrates, and the liquid concentrator spraying drying is promptly obtained donkey-hide gelatin peptide dry product;
The temperature and time of described activated carbon decolorizing is respectively 55 ℃ and 1h;
The membrane pore size of described membrane sepn is 1nm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN 201010540517 CN102021215B (en) | 2010-11-11 | 2010-11-11 | Method for extracting gelatin peptides |
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| Application Number | Priority Date | Filing Date | Title |
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| CN 201010540517 CN102021215B (en) | 2010-11-11 | 2010-11-11 | Method for extracting gelatin peptides |
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| CN102021215A CN102021215A (en) | 2011-04-20 |
| CN102021215B true CN102021215B (en) | 2012-12-12 |
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| CN 201010540517 Expired - Fee Related CN102021215B (en) | 2010-11-11 | 2010-11-11 | Method for extracting gelatin peptides |
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Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102277406A (en) * | 2011-08-18 | 2011-12-14 | 江中药业股份有限公司 | Method for preparing donkey-hide gelatin peptide |
| CN102389071B (en) * | 2011-11-10 | 2013-03-20 | 河南辅仁堂制药有限公司 | Donkey-hide gelatin raw powder and its preparation method |
| CN106360696B (en) * | 2016-09-18 | 2019-12-24 | 大连普瑞康生物技术有限公司 | A food composition containing saussurea involucrata culture and nidus Collocaliae |
| CN107712157A (en) * | 2017-11-14 | 2018-02-23 | 安徽华健生物科技有限公司 | A kind of processing method of donkey-hide gelatin ginger tea |
| CN107998333B (en) * | 2017-12-13 | 2021-01-29 | 国药肽谷有限公司 | Preparation method of donkey-hide gelatin peptide product |
| CN108300752B (en) * | 2018-04-03 | 2022-04-01 | 济南大学 | Method for preparing micromolecule donkey-hide gelatin peptide by using donkey-hide gelatin lumps |
| CN109925209A (en) * | 2019-03-13 | 2019-06-25 | 济南大学 | A kind of light peptide facial mask preparation method of donkey-hide gelatin |
| CN112971128A (en) * | 2021-03-30 | 2021-06-18 | 武汉博识科技有限公司 | Anti-cancer anti-aging nutritional tablet |
| CN115474692A (en) * | 2021-06-16 | 2022-12-16 | 法尔玛国际健康管理有限公司 | Nutritional composition for conditioning female endocrine function |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101269090A (en) * | 2008-04-21 | 2008-09-24 | 北京化工大学 | Method of preparing low molecular weight colla carapaeis corii asini hydrolysate |
| CN101285087A (en) * | 2008-04-30 | 2008-10-15 | 北京华达杰瑞生物技术有限公司 | Polypeptides with donkey hide gelatin and method for preparing same |
| CN101294186A (en) * | 2008-04-03 | 2008-10-29 | 山东中医药大学 | Method for preparing donkey-hide gelatin oligopeptide |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101294186A (en) * | 2008-04-03 | 2008-10-29 | 山东中医药大学 | Method for preparing donkey-hide gelatin oligopeptide |
| CN101269090A (en) * | 2008-04-21 | 2008-09-24 | 北京化工大学 | Method of preparing low molecular weight colla carapaeis corii asini hydrolysate |
| CN101285087A (en) * | 2008-04-30 | 2008-10-15 | 北京华达杰瑞生物技术有限公司 | Polypeptides with donkey hide gelatin and method for preparing same |
Non-Patent Citations (2)
| Title |
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| 付英杰等.阿胶低肽酶解工艺的实验研究.《中成药》.2010,第32卷(第1期),第143-146页. |
| 阿胶低肽酶解工艺的实验研究;付英杰等;《中成药》;20100131;第32卷(第1期);第143-146页 * |
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