CN101974590B - Method for extracting rice peptides - Google Patents
Method for extracting rice peptides Download PDFInfo
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- CN101974590B CN101974590B CN 201010540486 CN201010540486A CN101974590B CN 101974590 B CN101974590 B CN 101974590B CN 201010540486 CN201010540486 CN 201010540486 CN 201010540486 A CN201010540486 A CN 201010540486A CN 101974590 B CN101974590 B CN 101974590B
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Abstract
The invention discloses a method for extracting rice peptides. The method comprises the following steps of: adding water and ethanol into commercially available rice protein meal serving as a major raw material; adding sodium sulfite and raising the temperature for steaming; filtering; performing enzymolysis with complex enzyme; inactivating enzyme; de-coloring; performing membrane separation; and drying the obtained filtrate so as to obtain the rice peptides. The method of the invention has short production period and low cost and toxic and harmful substances are not produced; moreover, an obtained product has high safety and can be widely applied to fields such as foods, medicaments, health-care products and the like.
Description
Technical field
The inventive method relates to the deep process technology field of rice protein; Be specifically related to a kind of process for extracting of rice protein peptide; Be a kind of be raw material with the rice protein powder; Utilize enzyme enzymolysis rice protein and utilize the enzyme modification technique that protein peptide is modified, thereby extract the method for rice peptide.
Background technology
The paddy YO of China accounts for more than 1/3 of Gross World Product; Though in state-owned many grain processing plants, brewery, Spice Factory and starch sugar refinery etc.; But Chinese rice resource especially early rice, crack rice, ageing rice and produce seasonings and Dian Fentang after rice slag etc. fail to make full use of, cause their economic worth not find full expression.Utilize early rice, crack rice, exploitation rice protein such as ageing rice and rice slag and rice peptide be to make them be converted into the effective way of high value added product; Thereby receive the attention of country gradually, be put into one of content of country's 15 key scientific and technological projects (2001BA 501A 03) now.
Rice protein is a kind of quality plant albumen, can compare favourably with fish, shrimp and beef.What is more important, rice protein has hypoallergenic, can be used for dispensed food for baby.But the protein in the rice protein mainly is gluten, can only be dissolved in the basic soln, has therefore limited its application.Compare with rice protein; Rice peptide is except having above-mentioned advantage; Also has the not available good solubility of rice protein, acid, thermostability, physico-chemical property such as viscosity is low; And have and different physiological roles such as be prone to absorb, hypotensive, anti-oxidant, can be used as food raw material or auxiliary material and be applied to infantile nutrition formula food, ready-to-eat, instant drink and seasonings etc.; And because rice peptide has various physiologically actives; Also can be used for nutrition dietetic food, functional food and sportsmen's food etc.; Therefore, rice peptide is the promising functional food ingredient of a kind of ten minutes, in foodstuffs industry, has purposes and wide development application prospect widely.
The existing rice peptide that from rice protein powder, extracts is all accomplished through following steps mostly: rice protein powder → alkali alcohol processing → high temperature steaming → enzymolysis → filtration → concentrate → spraying drying.Adopt a large amount of alkali and organic solvent to handle, both brought serious environmental to pollute and potential safety hazard, destroyed proteinic composition again; And do not use membrane separation technique in the prior art, technology is loaded down with trivial details and energy consumption is high, has influenced economic benefit of enterprises.
Summary of the invention
To the deficiency that exists in the prior art, the object of the present invention is to provide a kind of process for extracting of rice peptide, be a kind of alkaline purification that need not to use; And utilize the enzyme enzymolysis; And further modify the peptide bond debitterize, with concentrated, extract the method for rice peptide with the membrane separation technique desalination.
In order to realize above-mentioned technical purpose, the step of the process for extracting of a kind of rice peptide of the present invention is following:
1, took by weighing rice protein powder after the 40 mesh sieve removal of impurities as raw material; The 6-7 that adds raw material rice protein powder weight successively doubly, zero(ppm) water, 95wt% ethanol and the sodium sulphite anhydrous 99.3 of 6-9% and 1-2% stir; At 90-95 ℃ of refluxed reaction 3-5h, filtered while hot must be filtrated then;
2, cooling is filtrated to 50-60 ℃, and adjust pH is 7-8, and the 2-4% that presses raw material rice protein powder weight adds Sumizyme MP, stirs enzymolysis 4-6h, and keeping the pH value of reaction system in the enzymolysis process is 7-8; The 1-2% that presses raw material rice protein powder weight again adds flavor protease, stirs enzymolysis 5-8h, and keeping the pH value of reaction system in the enzymolysis process is 7-8; The enzymolysis solution that obtains is warming up to 90-100 ℃, the insulation 10-20min enzyme that goes out;
3, with the centrifugal after-filtration of enzymolysis solution, remove proteolytic enzyme, the 2-2.5% that presses raw material rice protein powder weight in the filtrating adds powdered active carbon, and 55 ℃ of whip attachment 1h decolourings remove by filter gac then, collect filtrating;
4, filtrating uses the membrane sepn means to separate, and desalination also concentrates, and gets liquid concentrator;
5, promptly get the rice peptide dry product after the liquid concentrator drying.
The Sumizyme MP that uses in the inventive method and the enzyme activity of flavor protease are respectively: 25U/mg and 30U/mg.
Compared with prior art, advantage of the inventive method and beneficial effect are following:
1, the rice peptide produced of the inventive method, the peptide content of molecular weight below 1000 dalton reaches more than 98% in its gross protein, not only improved the nutritive value of rice, brought economic benefit for society simultaneously;
2, the rice peptide produced of the inventive method, molecular weight is little, and human body is prone to absorb, and specific absorption reaches more than 90%;
3, the inventive method has adopted activated carbon decolorizing, has guaranteed the natural whiteness of product, and products obtained therefrom is white uniform powder;
4, adopt membrane separation technique of the present invention, the liquid towards material separates, concentrates and purifying, all is to operate, do not have phase-state change, energy-efficient at normal temperatures, and does not produce pollution in the production process;
5, the inventive method is with short production cycle, cost is low, do not produce any hazardous and noxious substances, and safety has no side effect, and can be widely used in fields such as makeup, food and medicine.
Embodiment
Through concrete embodiment the inventive method is done further detailed description below.
Embodiment 1:
A kind of process for extracting of rice peptide, its step is following:
Took by weighing commercially available rice protein powder behind 40 mesh sieves (crude protein＞80wt% wherein; Moisture content＜10wt%, ash＜3wt%, the 1kg of crude fat＜6wt%); Add 7kg zero(ppm) water, 60g 95wt% ethanol and 10g sodium sulphite anhydrous 99.3; After stirring, be warming up to 90 ℃ of backflow 5h, filtered while hot then; Cooling filtrating is transferred pH to 7-8 to 50 ℃ with the food grade hydroxide flake sodium solution of 0.1M again, adds the 20g Sumizyme MP then, and 50 ℃ are stirred enzymolysis 6h, uses the sodium hydroxide solution of above-mentioned 0.1M to keep the pH value of reaction system to be 7-8 in the enzymolysis process; The flavor protease that adds 10g again, 50 ℃ are stirred enzymolysis 8h, and the sodium hydroxide solution with above-mentioned 0.1M in the enzymolysis process keeps pH value of reaction system at 7-8; Be warming up to 90 ℃ then, the insulation 20min enzyme that goes out; With the centrifugal after-filtration of enzymolysis solution, get supernatant then; Add the 20g powdered active carbon in the supernatant, 55 ℃ of whip attachment 1h decolourings, filtering gac; The filtrating via hole diameter is that the nanofiltration membrane separation system of 1nm separates, and desalination also concentrates; Liquid concentrator is spray-dried, obtains the Powdered rice peptide of 420g white (adopt activated carbon decolorizing among the present invention, the color and luster of products obtained therefrom is a white, is superior to the faint yellow of national Specification), and the examining report of products obtained therefrom is seen table 1.
The examining report of table 1 embodiment 1 products obtained therefrom
Embodiment 2:
A kind of process for extracting of rice peptide, its step is following:
Took by weighing commercially available rice protein powder behind 40 mesh sieves (crude protein＞80wt% wherein; Moisture content＜10wt%, ash＜3wt%, the 2kg of crude fat＜6wt%); Add 15kg zero(ppm) water, 180g 95wt% ethanol and 30g sodium sulphite anhydrous 99.3; After stirring, be warming up to 92 ℃ of backflow 4h, filtered while hot then; Cooling filtrating is transferred pH to 7-8 to 55 ℃ with the food grade hydroxide flake sodium solution of 0.1M again, adds the 60g Sumizyme MP then, and 55 ℃ are stirred enzymolysis 5h, uses the sodium hydroxide solution of above-mentioned 0.1M to keep the pH value of reaction system to be 7-8 in the enzymolysis process; Add the 30g flavor protease again, 55 ℃ are stirred enzymolysis 7h, and the sodium hydroxide solution with above-mentioned 0.1M in the enzymolysis process keeps pH value of reaction system at 7-8; Be warming up to 94 ℃ then, the insulation 16min enzyme that goes out; With the centrifugal after-filtration of enzymolysis solution, get supernatant then; Add the 45g powdered active carbon in the supernatant, 55 ℃ of whip attachment 1h decolourings, filtering gac; The filtrating via hole diameter is that the nanofiltration membrane separation system of 1nm separates, and desalination also concentrates; Liquid concentrator is spray-dried, obtains 838g white powder rice peptide, and the examining report of products obtained therefrom is seen table 2.
The examining report of table 2 embodiment 2 products obtained therefroms
Embodiment 3:
A kind of process for extracting of rice peptide, its step is following:
Took by weighing commercially available rice protein powder behind 40 mesh sieves (crude protein＞80wt% wherein; Moisture content＜10wt%, ash＜3wt%, the 10kg of crude fat＜6wt%); The ethanol and the 200g sodium sulphite anhydrous 99.3 that add 60kg zero(ppm) water, 700g 95wt%; After stirring, be warming up to 93 ℃ of backflow 4h, filtered while hot then; Cooling filtrating is transferred pH to 7-8 to 55 ℃ with the food grade hydroxide flake sodium solution of 0.1M again, adds the 400g Sumizyme MP then, and 55 ℃ are stirred enzymolysis 5h, uses the sodium hydroxide solution of above-mentioned 0.1M to keep the pH value of reaction system to be 7-8 in the enzymolysis process; Add the 200g flavor protease again, 55 ℃ are stirred enzymolysis 6h, and the sodium hydroxide solution with above-mentioned 0.1M in the enzymolysis process keeps pH value of reaction system at 7-8; Be warming up to 96 ℃ then, the insulation 14min enzyme that goes out; With the centrifugal after-filtration of enzymolysis solution, get supernatant then; Add the 250g powdered active carbon in the supernatant, 55 ℃ of whip attachment 1h decolourings, filtering gac; The filtrating via hole diameter is that the nanofiltration membrane separation system of 1nm separates, and desalination also concentrates; Liquid concentrator is spray-dried, obtains 4.22kg white powder rice peptide, and the examining report of products obtained therefrom is seen table 3.
The examining report of table 3 embodiment 3 products obtained therefroms
Embodiment 4:
A kind of process for extracting of rice peptide, its step is following:
Took by weighing commercially available rice protein powder behind 40 mesh sieves (crude protein＞80wt% wherein; Moisture content＜10wt%, ash＜3wt%, the 100kg of crude fat＜6wt%); Add 650kg zero(ppm) water, 8kg 95wt% ethanol and 1.6kg sodium sulphite anhydrous 99.3; After stirring, be warming up to 95 ℃ of backflow 3h, filtered while hot then; Cooling filtrating is transferred pH to 7-8 to 60 ℃ with the food grade hydroxide flake sodium solution of 0.1M again, adds the 3kg Sumizyme MP then, and 60 ℃ are stirred enzymolysis 4h, uses the sodium hydroxide solution of above-mentioned 0.1M to keep the pH value of reaction system to be 7-8 in the enzymolysis process; Add the 1.6kg flavor protease again, 60 ℃ are stirred enzymolysis 5h, and the sodium hydroxide solution with above-mentioned 0.1M in the enzymolysis process keeps pH value of reaction system at 7-8; Be warming up to 100 ℃ then, the insulation 10min enzyme that goes out; With the centrifugal after-filtration of enzymolysis solution, get supernatant then; Add the 2.2kg powdered active carbon in the supernatant, 55 ℃ of whip attachment 1h decolourings, filtering gac; The filtrating via hole diameter is that the nanofiltration membrane separation system of 1nm separates, and desalination also concentrates; Liquid concentrator is spray-dried, obtains 42.3kg white powder rice peptide, and the examining report of products obtained therefrom is seen table 4.
The examining report of table 4 embodiment 4 products obtained therefroms
Claims (4)
1. the step of the process for extracting of a rice peptide is following:
1), takes by weighing rice protein powder after the removal of impurities of sieving as raw material; Adding raw material rice protein powder weight 6-7 zero(ppm) water, the 95wt% ethanol of 6-9% and the sodium sulphite anhydrous 99.3 of 1-2% doubly successively stirs; At 90-95 ℃ of refluxed reaction 3-5h, filtered while hot must be filtrated then;
2), cooling filtrates to 50-60 ℃, adjust pH is 7-8, the 2-4% that press raw material rice protein powder weight adds the Sumizyme MP of 25U/mg, stirs enzymolysis 4-6h, the pH value of maintenance reaction system is 7-8 in the enzymolysis process; The 1-2% that presses raw material rice protein powder weight again adds the flavor protease of 30U/mg, stirs enzymolysis 5-8h, and keeping the pH value of reaction system in the enzymolysis process is 7-8; The enzymolysis solution that obtains is warming up to 90-100 ℃, the insulation 10-20min enzyme that goes out;
3), with the centrifugal after-filtration of enzymolysis solution, remove proteolytic enzyme, the 2-2.5% that presses raw material rice protein powder weight in the filtrating adds the powdered active carbon adsorption bleaching, removes by filter gac then, collects filtrating;
4), filtrating uses the membrane sepn means to separate, desalination also concentrates, liquid concentrator;
5), promptly get the rice peptide dry product after the liquid concentrator drying;
Described film is that the aperture is the nf membrane of 1nm.
2. the method for claim 1 is characterized in that: described sieving to crossing 40 mesh sieves.
3. the method for claim 1, it is characterized in that: the temperature and time of described decolouring is respectively 55 ℃ and 1h.
4. the method for claim 1, it is characterized in that: described drying is a spraying drying.
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| CN 201010540486 CN101974590B (en) | 2010-11-11 | 2010-11-11 | Method for extracting rice peptides |
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| CN 201010540486 CN101974590B (en) | 2010-11-11 | 2010-11-11 | Method for extracting rice peptides |
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| CN101974590A CN101974590A (en) | 2011-02-16 |
| CN101974590B true CN101974590B (en) | 2012-07-25 |
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Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102212107B (en) * | 2011-04-12 | 2013-01-02 | 江南大学 | Rice protein polypeptide and preparation method thereof |
| CN102524736B (en) * | 2011-12-27 | 2013-05-29 | 广州合诚实业有限公司 | Method for preparing functional flavor-developing base material from rice protein |
| CN102599504A (en) * | 2012-03-15 | 2012-07-25 | 河南省南街村（集团）有限公司 | Production process of ternary compound peptide powder |
| CN103250871A (en) * | 2013-05-22 | 2013-08-21 | 江苏联海生物科技有限公司 | Hydrolysis process of rice protein |
| CN103416592A (en) * | 2013-08-27 | 2013-12-04 | 江苏康科食品工程技术有限公司 | Industrial production method of rice protein hydrolysate for feed |
| CN103789383A (en) * | 2014-03-11 | 2014-05-14 | 江苏联海生物科技有限公司 | Rice peptide production method |
| CN103918869A (en) * | 2014-04-24 | 2014-07-16 | 盘锦和田食品有限公司 | High-purity rice protein peptide and preparation method thereof |
| CN104026329B (en) * | 2014-06-24 | 2016-03-30 | 江西省科学院应用化学研究所 | A kind of extracting method of natural high-content selenium source rice peptide |
| CN105441520A (en) * | 2015-11-23 | 2016-03-30 | 华中农业大学 | Method adopting rice residues as raw material for enzyme-membrane combined preparation of rice polypeptides |
| CN108504710A (en) * | 2018-03-29 | 2018-09-07 | 广西民族大学 | A kind of rice protein Gly-His-Lys and preparation method thereof |
| CN110029139A (en) * | 2019-01-31 | 2019-07-19 | 山东大树达孚特膳食品有限公司 | A kind of production technology of rice biologically active peptide |
| CN110574863A (en) * | 2019-08-28 | 2019-12-17 | 武汉轻工大学 | Method for processing deproteinized rice and products thereof for combined preparation of alpha-amylase inhibitor and protein peptide |
| CN114807282A (en) * | 2022-06-07 | 2022-07-29 | 湖北瑞邦生物科技有限公司 | Rice peptide with low bitter taste and antioxidant and anti-fatigue effects and preparation method thereof |
-
2010
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Non-Patent Citations (3)
| Title |
|---|
| 吴泽柱.玉米蛋白肽的生产加工研究现状.《中国食品添加剂》.2009, * |
| 陈季旺.大米肽的酶法制备工艺及其特性的研究.《农业工程学报》.2006, * |
| 韩素芳.酶在大米深加工中的应用.《食品研究与开发》.2007, * |
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