CN104026329B - A kind of extracting method of natural high-content selenium source rice peptide - Google Patents
A kind of extracting method of natural high-content selenium source rice peptide Download PDFInfo
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Abstract
The present invention relates to selenium source rice peptide extractive technique feed additive field, specifically disclose a kind of extracting method of natural high-content selenium source rice peptide, comprise the steps: to take pulverizing, sieve after selenium-rich rice, add rice weight 9-12 distilled water doubly, add the flavor protease of the 2%-4% of rice weight, put into ultrasonic extractor enzymolysis 4-6h, in enzymolysis process, keep the pH value of reaction system to be 7-8<b>; After </b> enzymolysis terminates, the enzymolysis liquid obtained is warming up to 90-100 DEG C, insulation 5-10min goes out enzyme; Enzymolysis liquid is centrifugal, and filtrate separation uses membrane separation technique purifying, obtains concentrate; Concentrate spraying dry, obtains high-load selenium source rice peptide.The present invention does not add acid-base solution, maintains the activity of selenium, and the content of Organic Selenium in product is improved, simultaneously can not to environment; Use membrane separation technique to enzymolysis liquid separation and purification, simple process, cost is low.
Description
Technical field
The present invention relates to selenium source rice peptide extractive technique field, be specifically related to a kind of extracting method of natural high-content selenium source rice peptide.
Background technology
Selenium has anticancer, cardioprotection, treating liver necrosis, control myopia and cataract, removing toxic substances, raising immunity, delays senility and strengthen the multiple pharmacological effect such as reproductive function, is described as " life kindling material ", " patron saint of heart " and " anticancer king ".The whole world has more than 40 countries and regions to belong to scarce selenium area.China is a Ge Que selenium big country, discloses, tiltedly wear to the Yunnan-Guizhou Plateau from three provinces in the northeast of China according to " People's Republic of China's localized disease and environmental factor atlas ", and the area accounting for China's area 72% exists a low selenium area, and wherein 30% is serious scarce selenium area.The big and medium-sized cities of northeast, North China, selenium north, southern, the south China of selenium, East China, the Delta of the Pearl River, Yangtze River Delta etc. all belong to low selenium area.At present, in population of China food, still do not reach the minimum selenium absorption level of more than 40 micrograms for each person every day, especially lack that selenium area crowd is more difficult reaches the object of mending selenium from food.If trace elements and health association of China director is longer than wood and points out: " China is a Ge Que selenium big country, human body mend selenium be related to hundreds of millions of Chinese people major issue we should do a good job of it as enriching the iodine mend selenium work ".
At present, supplement inorganic selenium hydrochlorate in domestic most employing salt, or adopt selenate to spray plant in the poplar bloassom phase, make rice, tealeaves etc. containing selenium, but these all belong to artificial inorganic selenium hydrochlorate, human body is difficult to absorb, and biological value is low.Meanwhile, the developed country such as Japan and the United States has forbidden in food, add the nutritional supplement as selenium such as sodium selenite.
Fengcheng is described as " Ecology in China selenium paddy ", and have good natural selenium resource base, its Se content is moderate, and its soil is different from high Se content area, can avoid the too high harm caused health of Se content completely.The selenium-rich soil resource area of Fengcheng City distribution is comparatively large, belongs to the selenium-rich soil that river selenium economizes Late Cambrian.Se from Soil content 0.12 ~ 0.99 μ g/g, average content 0.28 μ g/g, most soil belongs to selenium-rich soil.Detect through the sampling of Jiang Xisheng geological survey and research institute and find, Fengcheng City has 37 samples of 18 agricultural product, and without any technical finesse, plantation product just reaches Se-rich farm products standard.Further, show after deliberation, selenium mainly combines with rice peptide by soil mobility to rice, forms organic selenoprotein peptide.
Existingly extract mostly all to have been come by following steps containing selenium rice peptide from selenium-rich rice: heavy → precipitation → enzymolysis → filtration that selenium-rich rice → alkali proposes acid → concentrate → spraying dry.Have employed a large amount of alkali and have acid treatment, both having destroyed the combination of selenium and peptide, reduced the activity of selenium, the content of Organic Selenium in product is reduced; Meanwhile, serious environmental pollution and potential safety hazard; And seldom use membrane separation technique in prior art, technique is loaded down with trivial details and energy consumption is high, have impact on the economic benefit of enterprise.
Summary of the invention
The problem to be solved in the present invention is that existing selenium-rich rice peptide extracting method selenium activity is low, organic selenium content is few, environmental pollution is serious, consume energy high defect, a kind of extracting method of natural high-content selenium source rice peptide is provided, it uses ultrasonic extractor directly to get by water extraction, do not destroy the activity of selenium, product organic selenium content is high, can not cause environmental pollution; Energy consumption is low, and cost is low.
For achieving the above object, the technical solution adopted in the present invention is:
An extracting method for natural high-content selenium source rice peptide, it comprises the steps:
Take pulverizing, sieve after selenium-rich rice, add rice weight 9-12 distilled water doubly, add the flavor protease of the 2%-4% of rice weight, put into ultrasonic extractor enzymolysis 4-6h, in enzymolysis process, keep the pH value of reaction system to be 7-8; After enzymolysis terminates, the enzymolysis liquid obtained is warming up to 90-100 DEG C, insulation 5-10min goes out enzyme; Enzymolysis liquid is centrifugal, and filtrate separation uses membrane separation technique purifying, obtains concentrate; Concentrate spraying dry, obtains high-load selenium source rice peptide.
Preferably, described filtrate separation and purification adopts Ultra filtration membrane purifying.
Preferably, described milipore filter is the milipore filter of molecular weight 5000.
Being improved to further technique scheme, the step selecting enzyme was also had: the flavor protease of the enzyme activities such as utilization, compound protease, alkali protease, papain enzymolysis selenium-rich rice under respective optimum temperature and pH condition before taking selenium-rich rice, measure the Se content in respective enzymolysis liquid, what Se content was the highest is best enzyme.
Preferably, the step of described selection enzyme, specifically: take pulverizing, selenium-rich rice after sieving is raw material, add the distilled water of raw material weight 10 times, the 30U/mg flavor protease of enzyme activity such as to add again respectively, 40U/mg compound protease, 25U/mg alkali protease, 40U/mg papain, put into ultrasonic extractor, enzymolysis 5h under respective optimum temperature and pH condition respectively, the each enzymolysis liquid obtained is warming up to 100 DEG C, insulation 10min goes out enzyme, by centrifugal for enzymolysis liquid rear filtration, measure Se content in respective filtrate, determine that best enzyme type is flavor protease.
Preferably, the enzyme activity of described flavor protease is 5000U.
Preferably, the hydrolysis temperature of described flavor protease is 35 DEG C, keeps the pH value of reaction system to be 7 in enzymolysis process.
Preferably, in described ultrasonic extractor, enzymolysis time is 5h.
Preferably, described enzymolysis liquid is warming up to 95 DEG C.
Preferably, described enzymolysis liquid insulation 10min goes out enzyme.
The extracting method of natural high-content selenium source rice peptide of the present invention, its beneficial effect is:
Natural selenium-rich rice utilizes water to make solvent, extracts in ultrasonic extractor, does not add acid-base solution, can not destroy the combination of selenium and peptide, maintains the activity of selenium, and the content of Organic Selenium in product is improved, simultaneously also can not to environment; Use membrane separation technique to enzymolysis liquid separation and purification, simple process, cost is low, has obvious economic benefit.
Detailed description of the invention
For making the object, technical solutions and advantages of the present invention clearly, below in conjunction with accompanying drawing, the present invention is described in further detail.
Embodiment 1:
The extracting method of natural high-content selenium source rice peptide of the present invention, it comprises the steps:
Take pulverizing, sieve after natural selenium-rich rice be raw material, after adding the distilled water of raw material weight 10 times, add the 30U/mg flavor protease of 5000U enzyme activity, 40U/mg compound protease, 25U/mg alkali protease, 40U/mg papain respectively, put into ultrasonic extractor, enzymolysis 4h under respective optimum temperature and pH condition respectively, the each enzymolysis liquid obtained is warming up to 100 DEG C, and insulation 10min goes out enzyme; By centrifugal for enzymolysis liquid rear filtration, measure Se content in respective filtrate, determine that best enzyme type is flavor protease.
Take pulverizing, sieve after natural selenium-rich rice be raw material, add raw material weight 9-12 distilled water doubly, the flavor protease of 5000U vigor is added by the 2%-4% of raw material weight, put into ultrasonic extractor enzymolysis 4-6h, remain in enzymolysis process that the pH value of reaction system is 7.0, temperature is 35 DEG C; After enzymolysis terminates, the enzymolysis liquid obtained is warming up to 90-100 DEG C, insulation 5-10min goes out enzyme.
Enzymolysis liquid is centrifugal, and filtrate adopts the Ultra filtration membrane purifying of molecular weight 5000, obtains concentrate.
Namely natural high-content selenium source rice peptide dry product is obtained after concentrate is spray-dried.
Se content in table 1 enzymolysis liquid
Embodiment 2:
The extracting method of natural high-content selenium source rice peptide of the present invention, it comprises the steps:
Take pulverizing, sieve after natural selenium-rich rice 10g be raw material, after adding the distilled water of raw material weight 10 times, add the 30U/mg flavor protease of 5000U enzyme activity, 40U/mg compound protease, 25U/mg alkali protease, 40U/mg papain respectively, put into ultrasonic extractor, enzymolysis 4h under respective optimum temperature and pH condition respectively, the each enzymolysis liquid obtained is warming up to 100 DEG C, and insulation 10min goes out enzyme; By centrifugal for enzymolysis liquid rear filtration, measure Se content in respective filtrate, in table 1, because after flavor protease enzymolysis, Se content is the highest, determine that best enzyme type is flavor protease.
Take pulverizing, sieve after natural selenium-rich rice 1kg be raw material, add the distilled water of 9kg, add the flavor protease of 20g5000U vigor, put into ultrasonic extractor and stir enzymolysis 4h, remain in enzymolysis process that the pH value of reaction system is 7.0, temperature is 35 DEG C; After enzymolysis terminates, the enzymolysis liquid obtained is warming up to 95 DEG C, insulation 8min goes out enzyme.
Enzymolysis liquid is centrifugal, and filtrate adopts the Ultra filtration membrane purifying of molecular weight 5000, obtains concentrate.
Obtain 45.6g pale yellow powder after concentrate is spray-dried and be natural high-content selenium source rice peptide dry product.Product is detected, the results are shown in Table 2.
The testing result of table 2 product
Embodiment 2:
The extracting method of natural high-content selenium source rice peptide of the present invention, it comprises the steps:
Take pulverizing, sieve after natural selenium-rich rice be raw material, after adding the distilled water of raw material weight 10 times, add the 30U/mg flavor protease of 5000U enzyme activity, 40U/mg compound protease, 25U/mg alkali protease, 40U/mg papain respectively, put into ultrasonic extractor, enzymolysis 4h is stirred respectively under respective optimum temperature and pH condition, the each enzymolysis liquid obtained is warming up to 100 DEG C, and insulation 10min goes out enzyme; By centrifugal for enzymolysis liquid rear filtration, measure Se content in respective filtrate, in table 1, determine that best enzyme type is flavor protease.
Take pulverizing, sieve after natural selenium-rich rice 1kg be raw material, add the distilled water of 11kg, add the flavor protease of 25g5000U vigor, put into ultrasonic extractor and stir enzymolysis 4.5h, remain in enzymolysis process that the pH value of reaction system is 7.0, temperature is 35 DEG C; After enzymolysis terminates, the enzymolysis liquid obtained is warming up to 90 DEG C, insulation 10min goes out enzyme.
Enzymolysis liquid is centrifugal, and filtrate adopts the Ultra filtration membrane purifying of molecular weight 5000, obtains concentrate.
Namely obtain 46.1g pale yellow powder after concentrate is spray-dried, be natural high-content selenium source rice peptide dry product.Product is detected, the results are shown in Table 3.
The testing result of table 3 product
Embodiment 3:
The extracting method of natural high-content selenium source rice peptide of the present invention, it comprises the steps:
Take pulverizing, sieve after natural selenium-rich rice be raw material, after adding the distilled water of raw material weight 10 times, add the 30U/mg flavor protease of 5000U enzyme activity, 40U/mg compound protease, 25U/mg alkali protease, 40U/mg papain respectively, put into ultrasonic extractor, enzymolysis 4h under respective optimum temperature and pH condition respectively, the each enzymolysis liquid obtained is warming up to 100 DEG C, and insulation 10min goes out enzyme; By centrifugal for enzymolysis liquid rear filtration, measure Se content in respective filtrate, in table 1, determine that best enzyme type is flavor protease.
Take pulverizing, sieve after natural selenium-rich rice 1kg be raw material, add the distilled water of 12kg, add the flavor protease of 35g5000U vigor, put into ultrasonic extractor enzymolysis 6h, remain in enzymolysis process that the pH value of reaction system is 7.0, temperature is 35 DEG C; After enzymolysis terminates, the enzymolysis liquid obtained is warming up to 95 DEG C, insulation 10min goes out enzyme.
Enzymolysis liquid is centrifugal, and filtrate adopts the Ultra filtration membrane purifying of molecular weight 5000, obtains concentrate.
Namely obtain 46.5g pale yellow powder after concentrate is spray-dried, be natural high-content selenium source rice peptide dry product.Product is detected, the results are shown in Table 4.
The testing result of table 4 product
Embodiment 4:
The extracting method of natural high-content selenium source rice peptide of the present invention, it comprises the steps:
Take pulverizing, sieve after natural selenium-rich rice be raw material, after adding the distilled water of raw material weight 10 times, add the 30U/mg flavor protease of 5000U enzyme activity, 40U/mg compound protease, 25U/mg alkali protease, 40U/mg papain respectively, put into ultrasonic extractor, enzymolysis 4h under respective optimum temperature and pH condition respectively, the each enzymolysis liquid obtained is warming up to 100 DEG C, and insulation 10min goes out enzyme; By centrifugal for enzymolysis liquid rear filtration, measure Se content in respective filtrate, in table 1, determine that best enzyme type is flavor protease.
Take pulverizing, sieve after natural selenium-rich rice 1kg be raw material, add the distilled water of 11.5kg, add the flavor protease of 40g5000U vigor, put into ultrasonic extractor and stir enzymolysis 5h, remain in enzymolysis process that the pH value of reaction system is 7.0, temperature is 35 DEG C; After enzymolysis terminates, the enzymolysis liquid obtained is warming up to 100 DEG C, insulation 5min goes out enzyme.
Enzymolysis liquid is centrifugal, and filtrate adopts the Ultra filtration membrane purifying of molecular weight 5000, obtains concentrate.
Namely obtain 45.1g pale yellow powder after concentrate is spray-dried, be natural high-content selenium source rice peptide dry product.Product is detected, the results are shown in Table 5.
The testing result of table 5 product
Usually, the content of the selenium source rice peptide utilizing acid-base solution to extract is at 65.0-82.5mg/kg, and the known selenium source rice peptide Se content utilizing extracting method of the present invention to extract is high compared to prior art, and therefore extraction efficiency is high.
The above is the preferred embodiment of the present invention; it should be pointed out that for those skilled in the art, under the premise without departing from the principles of the invention; can also make some improvements and modifications, these improvements and modifications are also considered as protection scope of the present invention.
Claims (8)
1. an extracting method for natural high-content selenium source rice peptide, is characterized in that: comprise the steps:
Take pulverizing, sieve after selenium-rich rice, add rice weight 9-12 distilled water doubly, add the flavor protease of the 2%-4% of rice weight, put into ultrasonic extractor enzymolysis 4-6h, in enzymolysis process, keep the pH value of reaction system to be 7-8;
After enzymolysis terminates, the enzymolysis liquid obtained is warming up to 90-100 DEG C, insulation 5-10min goes out enzyme;
Enzymolysis liquid is centrifugal, and filtrate separation uses membrane separation technique purifying, obtains concentrate;
Concentrate spraying dry, obtains high-load selenium source rice peptide;
Described filtrate separation and purification adopts Ultra filtration membrane purifying;
Described milipore filter is the milipore filter of molecular weight 5000.
2. the extracting method of natural high-content selenium source rice peptide according to claim 1, it is characterized in that: before taking selenium-rich rice, also have the step selecting enzyme: the flavor protease of the enzyme activities such as utilization, compound protease, alkali protease, papain enzymolysis selenium-rich rice under respective optimum temperature and pH condition, measure the Se content in respective enzymolysis liquid, what Se content was the highest is best enzyme.
3. the extracting method of natural high-content selenium source rice peptide according to claim 2, it is characterized in that: the step selecting enzyme, specifically: take pulverizing, selenium-rich rice after sieving is raw material, add the distilled water of raw material weight 10 times, the 30U/mg flavor protease of enzyme activity such as to add again respectively, 40U/mg compound protease, 25U/mg alkali protease, 40U/mg papain, put into ultrasonic extractor, enzymolysis 5h under respective optimum temperature and pH condition respectively, the each enzymolysis liquid obtained is warming up to 100 DEG C, insulation 10min goes out enzyme, by centrifugal for enzymolysis liquid rear filtration, measure Se content in respective filtrate, determine that best enzyme type is flavor protease.
4. the extracting method of natural high-content selenium source rice peptide according to claim 1, is characterized in that: the enzyme activity of described flavor protease is 5000U.
5. the extracting method of natural high-content selenium source rice peptide according to claim 1, is characterized in that: the hydrolysis temperature of described flavor protease is 35 DEG C, keeps the pH value of reaction system to be 7 in enzymolysis process.
6. the extracting method of natural high-content selenium source rice peptide according to claim 1, is characterized in that: in described ultrasonic extractor, enzymolysis time is 5h.
7. the extracting method of natural high-content selenium source rice peptide according to claim 1, is characterized in that: described enzymolysis liquid is warming up to 95 DEG C.
8. the extracting method of natural high-content selenium source rice peptide according to claim 1, is characterized in that: described enzymolysis liquid insulation 10min goes out enzyme.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107459563A (en) * | 2017-08-11 | 2017-12-12 | 广西壮族自治区农业科学院农业资源与环境研究所 | A kind of new depositing technology during extraction selenoprotein |
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| CN104472851B (en) * | 2014-11-24 | 2017-10-10 | 南京农业大学 | It is a kind of to be enzymatically treated the method that selenium-rich rice produces selenium rich of egg white powder |
| CN110150671A (en) * | 2018-03-02 | 2019-08-23 | 浙江东来天然生物制品有限公司 | A method of extracting organic selenium from selenium-rich crocodile meat |
| CN110089616A (en) * | 2019-05-14 | 2019-08-06 | 江西虔农农业股份有限公司 | A kind of processing method of selenium-rich hydrolysis rice protein cream and powder |
| CN110699412B (en) * | 2019-11-27 | 2022-09-09 | 广西壮族自治区农业科学院 | Method for extracting selenium polypeptide from selenium-rich passion fruit seeds |
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| CN101974590A (en) * | 2010-11-11 | 2011-02-16 | 湖北远成药业有限公司 | Method for extracting rice peptides |
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| CN1911062A (en) * | 2006-08-21 | 2007-02-14 | 吴永尧 | Vegetable source natural selenium-rich peptide, prepn. method and use thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107459563A (en) * | 2017-08-11 | 2017-12-12 | 广西壮族自治区农业科学院农业资源与环境研究所 | A kind of new depositing technology during extraction selenoprotein |
| CN107459563B (en) * | 2017-08-11 | 2020-06-19 | 广西壮族自治区农业科学院农业资源与环境研究所 | Precipitation process in selenoprotein extraction process |
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