CN105851926A - Manufacturing method for sea cucumber essence pure powder - Google Patents

Abstract

The invention relates to a manufacturing method for sea cucumber essence pure powder. The method comprises the following steps: first, grinding East China Sea black sea cucumbers which are subjected to decontaminating and cleaning treatment into pulp by using a stainless steel grinder; adding the pulp into a stainless steel enzymolysis tank through a 40-mesh screen; adding tap water with the weight being 1.8 to 2.0 times weight of the East China Sea black sea cucumbers; performing heat-insulating heating at 95 DEG C for half an hour; closing steam; starting an enzymolysis tank stirring machine, and enabling the enzymolysis tank stirring machine to rotate by 30 rotations per minute; turning on enzymolysis tank jacket cooling water to decrease the temperature of materials to 45 DEG C; adding Alcalase proteolytic enzyme with the weight being 0.1 to 0.5 percent of the weight of the East China Sea black sea cucumbers, Protamex compound protease with the weight being 0.1 to 0.5 percent of the weight of the East China Sea black sea cucumbers, and Flavourzyme with the weight being 0.1 to 0.5 percent of the weight of the East China Sea black sea cucumbers into the materials; starting the enzymolysis tank stirring machine, and performing heat-insulating hydrolysis for four hours at 45 to 47 DEG C; filtering and concentrating the materials after enzyme hydrolysis is ended, wherein the temperature of the materials is controlled to be 55 to 58 DEG C, and concentrating is not ended until the solid content is 25 to 30 percent; performing spray-drying by using a centrifugal spray-drying machine, thus obtaining a finished product, namely the sea cucumber essence pure powder, wherein the temperature at the inlet of the spray-drying machine is controlled to be 210 to 215 DEG C, and the temperature at the outlet of the spray-drying machine is controlled to be 95 to 96 DEG C.

Description

A kind of manufacture method of the pure powder of Stichopus japonicus elite

Technical field

The invention belongs to marine organisms new resources and utilize manufacture field, the manufacture method of a kind of pure powder of Stichopus japonicus elite.

Background technology

It was found that there is Stichopus japonicus about kind more than 140 in China.Wherein more than 20 kind of Stichopus japonicus can be edible, common beche-de-mer have Radix Morinae Bulleyanae, Stichopus variegatus (Sempen), Stichopus chloronotus (Brandt)., hojothuria leucospilota, Thelenota ananas (Jaeger)．, white anus Acaudina molpadioides (Acaudina leucoprocta H.L.Clark), Serpentis Whitish eye Buddhist nun ginseng, figure stricture of vagina Bai Nican, trepang, actinopyga, spine trepang, black tuber of Codonopsis lanccolata Benth. Et Hook, Holothuria scabra, picolour zhuopianshen etc.. Black sea cucumbers from East China Sea mainly based on the white anus Acaudina molpadioides of wild black (having another name called: beche-de-mer in East China Sea or East Sea perfume (or spice) ginseng), body length maximum up to 400mm, generally 100-200mm, be distributed in Area of The East China Sea from Intertidal zone to the depth of water in the globigerina of 80 meters.

Recording and modern scientific research according to history records, Stichopus japonicus is a kind of excellent integration of edible and medicinal herbs, high protein and low fat food, has rich Rich nutrition.Black sea cucumbers from East China Sea is rich in arginine (more than 8%), although arginine is not one of eight kinds of essential amino acids of human body, But, owing to arginine aggregate velocity in human body is very slow, for growing rapid infant, it is still essential amino acids. Can be as the nutrition enhancer of infant food and male's anti-fatigue health-product containing through the refined pure powder of Stichopus japonicus elite purified Nutrition enhancer.Black sea cucumbers from East China Sea is also rich in holotoxin and Stichopus japonicus polysaccharide, all right through the refined pure powder of Stichopus japonicus elite purified Become the adjuvant drug improving leukocyte etc. after cancer patient improves immunity, irradiation.

In the refined pure powder of Stichopus japonicus elite purified, characteristic amino acid hydroxyproline and the glycine of animal collagen contains Amount up to more than 30%, is the food of a kind of high-quality, superior cosmetics, health product raw material.

According in March, 2000 Fujian Province, to Min Dong fishing ground, (sea area, place is positioned at coastal waters, the north, Fujian Province, in the range from 26 ° 00 ～27 ° of 00 N, marine site to the west of 125 ° of 00 E, area is about 16600 squares in the sea) benthon investigation, Fujian Fishing ground, east has benthon 72 kinds, white anus Acaudina molpadioides to account for 41.94%, and ribbon wirm accounts for 19.94%, and difference sun foot then accounts for 7.09%, Hook times spine ophiruid accounts for 6.24%, and only caterpillar accounts for 2.48%.

According to the Institute of Oceanology of the Chinese Academy of Sciences's in November, 2000 and April calendar year 2001 121 °～127 ° of E, between 26 °～32 ° of N " East Sea Distribution of Macrobenthos In Spring And Autumn " investigation that waters, the East Sea is done, detects 392 kinds of macrobenthos altogether. In these 392 kinds of zoobenthos, have 21 kinds distributed more widely in continental shelf district, quantity is relatively big, and occurrence rate is the highest, wherein comprises White anus Acaudina molpadioides.

Zhejiang, Fujian and Guangdong black sea cucumbers from East China Sea resource are the abundantest, and fisherman tends to drag tens of to thousands of when trawling Kilogram.Owing to this Stichopus japonicus contains abundant collagen protein, at high temperature can boil harder and harder, it is difficult to directly eat, this be also east Sea crow ginseng resource fails to be utilized effectively, abandoned one of reason returning sea as refuse always.According to relevant person's rough estimate, Only plant resources in Wenling city the most just has 30,000 tons to be abandoned, and the black sea cucumbers from East China Sea quantity in Qi Gui sea, PORT OF SHIPU, Xiangshan, Ningbo is the most surprising.Newly China society once with " Taizhou, Zhoushan perfume (or spice) ginseng aboundresources, marine expert appeal exploitation " for topic, it was recently reported that national marine fisheries expert Suggestion to exploitation beche-de-mer in East China Sea resource.

Japan the most all takes much count of the utilization of resources of black sea cucumbers from East China Sea, and the method that Japan's fishing boat uses fishing jig to fish fishes for east in a large number Sea crow ginseng, and become food of sea cucumber at seagoing fishing vessel work processing on ship.

China starts from early eighties in last century to the research on utilization of black sea cucumbers from East China Sea, although in Shanghai, the Deng Diyou East Sea, Wenzhou The product (East Sea perfume (or spice) ginseng) of crow ginseng occurs, but fails to realize industrialization the most always.In recent years, along with holothruian cultures heat In the rise in Bohai Sea Gulf, create the Stichopus japonicus industry of an annual value of production more than 100 hundred million, thus caused numerous research institutions to Stichopus japonicus The research of (including black sea cucumbers from East China Sea) nutritional and officinal compositions.Win according to Institute of Oceanology of the Chinese Academy of Sciences Liao Yu unicorn, Tsing-Hua University The expert such as rover station Du Li army and the Shanghai two easy Yang Hua in Large Marine Ecosystem institute of materia medica of army research after scholar, black sea cucumbers from East China Sea has abundant battalion Forming point and medical value, wherein the content of total amino acid content, holotoxin and Stichopus japonicus polysaccharide is all higher than topaz Stichopus japonicus.But, It is also proposed black sea cucumbers from East China Sea body surface schmutzband containing harmful heavy metals such as substantial amounts of lead, cadmium, hydrargyrum.Black sea cucumbers from East China Sea lives in seabed throughout the year In mud, body surface can secrete an a kind of harmful huge sum of money rich in aminoacid and in the mucus of collagen protein, this mucus and bottom silt Belong to firm to combine, define the body surface schmutzband of black sea cucumbers from East China Sea.The removing of this layer of dirt is extremely difficult, normal by commonly use at present Rule clean and add sodium hydroxide, hydrogen peroxide cleaning etc. process black sea cucumbers from East China Sea product be extremely difficult to country GB2733-2005 " fresh, Freeze animality aquatic products sanitary standard " and the requirement of Ministry of Agriculture NY/T 1514-2007 " pollution-free food Stichopus japonicus and goods " standard. In terms of the black sea cucumbers from East China Sea Product checking result that market is collected, harmful heavy metal exceedes national standard tens and arrives hundred times, and this is also Black sea cucumbers from East China Sea affluent resources fail to be utilized effectively and the basic reason place of industrialization.

The present invention is to use inventor granted patent (processing method of a kind of beche-de-mer in East China Sea, ZL 2,009 1 0098389.7) In removing black sea cucumbers from East China Sea body surface harmful heavy metal, a kind of sea cucumber essence proposed on the basis of solving black sea cucumbers from East China Sea food-safety problem The manufacture method of the pure powder of China.

Domestic Stichopus japonicus kind Radix Morinae Bulleyanae currently mainly is expensive, in main product ground Liaoning, the every 500 grams of cultivation in Shandong of Radix Morinae Bulleyanae The price of light dry Radix Morinae Bulleyanae is 6000-7000 yuan, and the price of wild light dry Radix Morinae Bulleyanae is more up to every 500 grams of 18000-30000 Unit, expensive cost of material limits the yield of the pure powder of Stichopus japonicus elite.And black sea cucumbers from East China Sea is the abundantest because of natural resources, application What people company produced meets country GB2733-2005 " fresh, freeze animality aquatic products sanitary standard " and Ministry of Agriculture NY/T Every 500 grams of the unsalted dried sea cucumbers of 1514-2007 " pollution-free food Stichopus japonicus and goods " standard-required is only 1100 yuans, makes The pure powder of Stichopus japonicus elite of high performance-price ratio becomes mass consumption and is achieved；

The amino acid content of the freshest wild black sea cucumbers from East China Sea be 12.4360 grams/100 grams (analyzing sample concentration is: 8.8680 mg/ml), the amino acid content of fresh artificial cultivation Radix Morinae Bulleyanae is 5.6643 grams/100 grams and (analyzes sample dense Degree is: 8.8500 mg/ml), the amino acid content ratio of wild black sea cucumbers from East China Sea in the case of analysis sample concentration is close The amino acid content of artificial cultivation Radix Morinae Bulleyanae doubles above.

Summary of the invention

It is an object of the invention to the deficiency overcoming prior art to exist, and the manufacture method of a kind of pure powder of Stichopus japonicus elite is provided.

It is an object of the invention to complete by following technical solution.The manufacture method of this pure powder of Stichopus japonicus elite, the method walks Rapid as follows:

(1), first, by through decontamination, the black sea cucumbers from East China Sea of cleaning treatment, slurry is worn into rustless steel fiberizer, by 40 mesh sieves Net, is added in rustless steel enzymatic vessel, adds the tap water of black sea cucumbers from East China Sea weight 1.8-2.0 times, opens enzymatic vessel chuck and add vapours, 95 DEG C of Heat preservation half an hour, steam off, open enzymatic vessel blender, 30 turns per minute, open enzymatic vessel folder jacket water Temperature of charge is dropped to 45 DEG C, in material add black sea cucumbers from East China Sea weight 0.1%-0.5% Alcalase hydrolysising protease, The Protamex compound protease of 0.1%-0.5%, the Flavourzyme flavor protease of 0.1%-0.5%, open enzymatic vessel and stir Mix machine, 30 turns per minute, open enzymatic vessel and add vapours, be incubated 45 DEG C-47 DEG C and hydrolyze 4 hours；

(2), to there being the manufacturing enterprise of drying machine with centrifugal spray, enzyme hydrolysis terminates rear material and passes through after filter press filter pressing again Membrane filtering machine fine filtering, the stainless-steel vacuum thickener of the material after fine filtering concentrates, and heats by controlling vacuum decker The temperature of charge that steam valve controls in vacuum decker is 55 DEG C-58 DEG C, is concentrated into solid content 25%-30%, then uses centrifugal spray Mist drying machine is spray-dried, and the inlet temperature controlling spray dryer is 210 DEG C-215 DEG C, and outlet temperature is 95 DEG C-96 DEG C, becomes Product are the pure powder of Stichopus japonicus elite, qualified are Stichopus japonicus elite pure powder product through inspection；

(3), to not having the manufacturing enterprise of drying machine with centrifugal spray, enzyme hydrolysis terminates rear material warp again after filter press filter pressing Crossing Membrane filtering machine fine filtering, the stainless-steel vacuum thickener of the material after fine filtering concentrates, and adds by controlling vacuum decker Temperature of charge in vapours Valve controlling vacuum decker is 55 DEG C-58 DEG C, is concentrated into solid content 45% 50% for paste, then Water content 3%-5% is dried in loading stainless steel disc, it is crushed to 80 mesh with rustless steel pulverizer and is Stichopus japonicus elite pure powder finished product, Qualified it is Stichopus japonicus elite pure powder product through inspection.

The invention have the benefit that the technology path that have employed three enzyme unifications, the enzymic hydrolysates making the pure powder of Stichopus japonicus elite is relative Molecular mass size is controlled, big to holothurian collagen product relative molecular mass with applicable food, cosmetics, health products trade Little requirement, product does not has bitterness and fishy smell.Solve and use the hydrolyzate such as papain, neutral protease to have hardship in the past Taste and fishy smell, the problem that relative molecular weight is bigger than normal, less than normal.Use the people that resourceful wild black sea cucumbers from East China Sea fictitious hosts is expensive Work culturing stichopus japonicus raw material, makes Stichopus japonicus elite this luxury goods of pure powder be become popular consumer goods.

Detailed description of the invention

Below by detailed description of the invention, the present invention is further elaborated, and embodiment will facilitate a better understanding of the present invention, but this Invention is not limited only to following embodiment.

Embodiment 1: Stichopus japonicus elite of the present invention pure powder manufacture method step is as follows:

(1), first by through the black sea cucumbers from East China Sea of decontamination, cleaning treatment, (processing method is shown in inventor's granted patent: Yi Zhongdong The processing method of sea Stichopus japonicus, ZL 2,009 1 0098389.7), wear into slurry with rustless steel fiberizer, by 40 eye mesh screens, add In rustless steel enzymatic vessel, add the tap water of black sea cucumbers from East China Sea weight 1.8-2.0 times, open enzymatic vessel chuck and add vapours, 95 DEG C Heat preservation half an hour, steam off, open enzymatic vessel blender, 30 turns per minute, open enzymatic vessel folder jacket water by thing Material temperature degree drops to 45 DEG C, adds the Alcalase hydrolysising protease of black sea cucumbers from East China Sea weight 0.1%-0.5%, 0.1%-0.5% in material Protamex compound protease, the Flavourzyme flavor protease of 0.1%-0.5%, above-mentioned protease Dou Shi Novi letter (in State) food-grade albumen enzyme that Investment Co., Ltd produces, open enzymatic vessel blender, 30 turns per minute, open enzymatic vessel heating Steam, is incubated 45 DEG C-47 DEG C and hydrolyzes 4 hours；

(2), enzyme hydrolysis terminates rear material after filter press filter pressing again through Membrane filtering machine fine filtering, after fine filtering Material stainless-steel vacuum thickener concentrates, and controls the material in vacuum decker by controlling vacuum decker heating steam valve Temperature is 55 DEG C-58 DEG C, is concentrated into solid content 45% 50% for paste, dries water content 3%-5% in reinstalling stainless steel disc, It is crushed to 80 mesh with rustless steel pulverizer and is Stichopus japonicus elite pure powder finished product, qualified be Stichopus japonicus elite pure powder product through inspection.

The present embodiment is applicable to the manufacturing enterprise not having drying machine with centrifugal spray.

Embodiment 2: the manufacture method step of the pure powder of Stichopus japonicus elite of the present invention is as follows:

(1), first by through the black sea cucumbers from East China Sea of decontamination, cleaning treatment, (processing method is shown in inventor's granted patent: Yi Zhongdong The processing method of sea Stichopus japonicus, ZL 2,009 1 0098389.7), wear into slurry with rustless steel fiberizer, by 40 eye mesh screens, add In rustless steel enzymatic vessel, add the tap water of black sea cucumbers from East China Sea weight 1.8-2.0 times, open enzymatic vessel chuck and add vapours, 95 DEG C Heat preservation half an hour, steam off, open enzymatic vessel blender, 30 turns per minute, open enzymatic vessel folder jacket water by thing Material temperature degree drops to 45 DEG C, adds the Alcalase hydrolysising protease of black sea cucumbers from East China Sea weight 0.1%-0.5%, 0.1%-0.5% in material Protamex compound protease, the Flavourzyme flavor protease of 0.1%-0.5%, above-mentioned protease Dou Shi Novi letter (in State) food-grade albumen enzyme that Investment Co., Ltd produces, open enzymatic vessel blender, 30 turns per minute, open enzymatic vessel heating Steam, is incubated 45 DEG C-47 DEG C and hydrolyzes 4 hours；

(2), enzyme hydrolysis terminates rear material after filter press filter pressing again through Membrane filtering machine fine filtering, after fine filtering Material stainless-steel vacuum thickener concentrates, and controls the material in vacuum decker by controlling vacuum decker heating steam valve Temperature is 55 DEG C-58 DEG C, is concentrated into solid content 25%-30%, then is spray-dried with centrifugal spray dryer, controls to be spray-dried The inlet temperature of machine is 210 DEG C-215 DEG C, and outlet temperature is 95 DEG C-96 DEG C, and finished product is the pure powder of Stichopus japonicus elite, qualified i.e. through inspection For Stichopus japonicus elite pure powder product.

The present embodiment is applicable to the manufacturing enterprise having drying machine with centrifugal spray.

In addition to the implementation, the present invention can also have other embodiments.The skill that all employing equivalents or equivalent transformation are formed Art scheme, all falls within the protection domain of application claims.

Claims (1)

1. the manufacture method of the pure powder of Stichopus japonicus elite, it is characterised in that: the method step is as follows:

(1), first, by through decontamination, the black sea cucumbers from East China Sea of cleaning treatment, slurry is worn into rustless steel fiberizer, by 40 mesh sieves Net, is added in rustless steel enzymatic vessel, adds the tap water of black sea cucumbers from East China Sea weight 1.8-2.0 times, opens enzymatic vessel chuck and add vapours, 95 DEG C of Heat preservation half an hour, steam off, open enzymatic vessel blender, 30 turns per minute, open enzymatic vessel folder jacket water Temperature of charge is dropped to 45 DEG C, in material add black sea cucumbers from East China Sea weight 0.1%-0.5% Alcalase hydrolysising protease, The Protamex compound protease of 0.1%-0.5%, the Flavourzyme flavor protease of 0.1%-0.5%, open enzymatic vessel and stir Mix machine, 30 turns per minute, open enzymatic vessel and add vapours, be incubated 45 DEG C-47 DEG C and hydrolyze 4 hours；

(2), to there being the manufacturing enterprise of drying machine with centrifugal spray, enzyme hydrolysis terminates rear material and passes through after filter press filter pressing again Membrane filtering machine fine filtering, the stainless-steel vacuum thickener of the material after fine filtering concentrates, and heats by controlling vacuum decker The temperature of charge that steam valve controls in vacuum decker is 55 DEG C-58 DEG C, is concentrated into solid content 25%-30%, then uses centrifugal spray Mist drying machine is spray-dried, and the inlet temperature controlling spray dryer is 210 DEG C-215 DEG C, and outlet temperature is 95 DEG C-96 DEG C, becomes Product are the pure powder of Stichopus japonicus elite, qualified are Stichopus japonicus elite pure powder product through inspection；

(3), to not having the manufacturing enterprise of drying machine with centrifugal spray, enzyme hydrolysis terminates rear material warp again after filter press filter pressing Crossing Membrane filtering machine fine filtering, the stainless-steel vacuum thickener of the material after fine filtering concentrates, and adds by controlling vacuum decker Temperature of charge in vapours Valve controlling vacuum decker is 55 DEG C-58 DEG C, is concentrated into solid content 45% 50% for paste, then Water content 3%-5% is dried in loading stainless steel disc, it is crushed to 80 mesh with rustless steel pulverizer and is Stichopus japonicus elite pure powder finished product, Qualified it is Stichopus japonicus elite pure powder product through inspection.