CN1891239B - Method for preparing Chinese herb medicine utilizing enzyme preparation - Google Patents
Method for preparing Chinese herb medicine utilizing enzyme preparation Download PDFInfo
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- CN1891239B CN1891239B CN2005102003753A CN200510200375A CN1891239B CN 1891239 B CN1891239 B CN 1891239B CN 2005102003753 A CN2005102003753 A CN 2005102003753A CN 200510200375 A CN200510200375 A CN 200510200375A CN 1891239 B CN1891239 B CN 1891239B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract
The present invention relates to a method for preparing Chinese herbal medicine by using enzyme preparation. It is extracted by utilizing biochemical action. It is characterized by that it utilizes catalystic reaction of enzyme and fermentation action of microorganism, according to different medicinal material or its composition, said invention selects the multienzyme body containing pectic enzyme, alpha-diastase and proteinase as enzyme preparation and utilizes said enzyme preparation to make the Chinese herbal medicine undergo the sufficient enzymolysis and fermentation so as to obtain the invented Chinese herbal medicine product. The Chinese herbal medicine according to the invention has a good curing effect, and has no pollution. It is convenient to use, rapid to effect, low cost and has no side effect. It has wide application prospect.
Description
Technical field
The present invention relates to the evaluation and exploration technology of enzyme in a kind of biological technical field, particularly a kind of method (promptly utilizing enzymolysis process from Chinese medicinal materials, to extract the method that effective medicinal component is grabbed functional substance) of utilizing the enzyme preparation herbal medicine.
Technical background
As everyone knows, Chinese materia medica is a great treasure-house of the Chinese nation, and the theory of medicine of holonomic system is not only arranged, and also has the prescription of the various illnesss of countless treatments, has comprised the various Chinese medicines of herbal medicine in these prescriptions.Herbal medicine derive from a large number the root, stem, leaf of plant, really, flower, its effective medicinal component mainly is the various natural chemical substances in the plant.But it is Mierocrystalline cellulose that 50-75% is arranged in the plant dry weight, the natural chemical substance of plant is present in the cell of plant cellulose or between the intercellular substance, and the extraction of Chinese medicine effective efficiency material is subjected to the very big restriction that the tradition of Chinese medicine production falls behind technology, at present both at home and abroad the Chinese medicine preparation method adopt usually traditional fry in shallow oil, boil, endure, soak, means such as pulverizing, what have replaces traditional frying in shallow oil, boil, endure with steam; The back soup that will decoct in the post-treatment of Chinese patent medicine that also has concentrates, dries, and pulverizes the Chinese patent medicine of making capsule, tablet, pulvis, pill again.Because the backwardness of Chinese medicine production traditional technology can only be extracted a small amount of effective ingredient in the medicinal material, causes the extraction yield of effective ingredient on the low side; Many useful VITAMIN and trace element, destroyed in high temperature, highly compressed production process, the volatilization of volatile oil cause the efficacy of a drug to weaken, and have influenced the raising of drug effect; In addition, wherein effective ingredient generally rests on the macromole state, can only play a role in the sick body extracellular, absorb slower, the corresponding reduction of curative effect; Simultaneously, also will consume a large amount of energy and manpower expense, produce " three wastes ", these all make the Chinese herbal medicine resource waste serious, and economic benefit and environmental protection are influenced.
Summary of the invention
The object of the present invention is to provide a kind of method (promptly utilizing enzymolysis process from Chinese medicinal materials, to extract the method for effective medicinal component) of utilizing enzyme preparation Chinese medicine, this method is utilized the catalyzed reaction of enzyme and the fermentative action of microorganism, herbal medicine is carried out sufficient enzymolysis, fermentation, farthest impel the release of Chinese medicinal materials effective efficiency material, increase substantially the extraction yield of effective medicinal component in the herbal medicine.
The present invention utilizes the method for enzyme preparation herbal medicine, it is characterized in that this method follows these steps to carry out: Chinese medicinal materials is added pure water, grab agglomerating on hand with medicinal material, decontroling promptly scatters is principle; Add weak acid and regulate pH to 4.5～6.5, soak after 8～12 hours, add pure water again and make Chinese medicinal materials become pasty state, adjust pH 4.0～5.0; Put in the enzymatic vessel by the multienzyme body mixed solution that adds 200～250mL in the dried medicinal material of per kilogram, under 30～50 ℃, carry out enzymolysis, fermentation 48～72h, stir oxygenation once every 5～10h; Enzymatic hydrolysis and fermentation carries out squeeze and filter after finishing, and washs some times and filters, and merging filtrate separates purification, and packaging sterilizing gets product.
Why the present invention adopts above-mentioned technical solution to be based on:
(1) utilizes the catalyzed reaction of enzyme and the fermentative action of microorganism, and according to different medicinal materials or different medicinal material compositions, selection is by based on bacterium cellulose oxalate enzyme, contain the multienzyme body of polygalacturonase, α-Dian Fenmei and proteolytic enzyme and the multienzyme body mixed solution of primary liquid formation simultaneously and herbal medicine is carried out sufficient enzymolysis, fermentation as enzyme preparation fermented liquid, and keep carrying out synchronously of fermentation and enzymolysis, to impel the effective efficiency material in the Chinese medicinal materials fully to be discharged, to improve extraction yield to effective medicinal component;
(2) be Mierocrystalline cellulose because of 50-75% is arranged in the plant dry weight, use, the medicinal component that is present between the interior or intercellular substance of vegetable cell is fully discharged based on the abundant degraded cellulose of the multienzyme body mixed solution of cellulase.In enzymolysis process, be also noted that the processing condition such as ratio of strict control pH value, temperature and multienzyme body and substrate (Chinese medicinal materials composition), create the control environment condition that fermentation and enzymolysis carry out synchronously, form the synergy of fermenting in the reaction process, farthest impel effective active matter fully to discharge in order to abundant extraction with enzymolysis.
(3) in multienzyme body mixed solution, why to adopt polygalacturonase, be because leaf, pericarp (as shaddock skin, orange peel) and some medicinal animal (as Melaphis chinensis (Bell) M. Paitan Tsaiet Tang) of nearly all medicinal plant all contain pectin, therefore great majority will have polygalacturonase to participate in and could prove effective more when the enzymolysis herbal medicine.
(4) according to the mechanism of action of enzyme, in multienzyme body mixed solution, add a certain amount of enzyme promotor and trace element, help the enzymolysis of microbial enzyme substrate; Fermenting mechanism according to microorganism, in multienzyme body mixed solution, add primary liquid, can be microbial growth breeding and fermentation essential carbon source, nitrogenous source, growth factor and optimum pH is provided, simultaneously for cellulase and other enzyme more effectively the coordinated enzymatic hydrolysis herbal medicine create favorable conditions: as utilizing the ganoderan in several enzyme extraction Ganoderma resinaceum Boud.s merely, its extraction yield is endured technology than traditional decoction and is improved 70%; And add enzyme promotor, primary liquid carries out enzymolysis, its extraction yield is 3 times of traditional technology, is 1.76 times of simple several enzyme enzymolysis process.
Than existing technology, the present invention has following remarkable advantage: the one, overcome the defective of traditional technology, effective constituent in the herbal medicine is fully extracted, the comparable conventional art of extraction yield improves 60-500%, and the waste residue in the method for tradition can being fried in shallow oil, boil, decocted Chinese medicine by boiling and simmering is converted into the material that glucose etc. easily is absorbed by the body in a large number, thereby help saving medicine source, the energy, reduce the pharmacy cost; The 2nd, the synergy of the enzymatic hydrolysis and fermentation by multienzyme body mixed solution can make the cellularstructure of Chinese medicinal materials change, and it is external to make intracellular effective medicinal ingredients be discharged into cell, thereby improves the extraction yield of medicinal ingredients; The 3rd, the herbal medicine good effect that makes, pollution-free, application convenience, having the quick-acting advantage of Western medicine concurrently does not but have its toxicity and side effect.
Embodiment
Embodiment one:
Ganoderma resinaceum Boud.: 1 kilogram.
Preparation multienzyme body mixed solution: cellulase 10g, polygalacturonase 5g, proteolytic enzyme 5g, peptone 3g, glucose 3g, lactic acid 10ml, yeast extract paste 3g, red colouring agent for food, also used as a Chinese medicine 5g, yeast 5g, water is made into 200ml.
The enzyme promotor that is added in the multienzyme body mixed solution: NaCl 2g, CaCl
22g, KCl 2g, MgSO
47H
2O 0.5g.
Get 1 kilogram of Ganoderma resinaceum Boud. in 50～60 ℃ of oven dry, be crushed to 20 orders, add 3 kilograms of pure water, regulate pH value 4.5～6.5 with lactic acid and soaked 8 hours, add 2 kilograms of pure water again, transfer PH to 4.0～5.0 to add 200ml multienzyme body mixed solution, stir, put in the enzymatic vessel 37 ℃ of enzymatic hydrolysis and fermentations 48 hours, and stirred oxygenation every day 3 times, carry out squeeze and filter after the end, filter residue filters for two times with 50～60 ℃ of pure water washings, merging filtrate concentrates and is dried into powder, and sterilising packaging is finished product.
Under the situation of identical glossy ganoderma consumption (1 kilogram), with above-mentioned finished product with traditional water boiling method and add the enzyme mixture method extract finished product use the content (by the detection of Fujian Province Institute of Analysis) of ultraviolet spectrophotometry ganoderan respectively, the result is as shown in the table:
| The tradition poach | The mixture enzymolysis of enzyme | Bacterium oxalic acid multienzyme body mixed solution enzymolysis | |
| Crude polysaccharides content (mg/g) | 70.6 | 85.7 | 119.6 |
| Extract gained paste quality (g) | 100 | 140 | 180 |
Embodiment two:
Radix Glycyrrhizae: 1 kilogram.
Preparation multienzyme body mixed solution: cellulase 10g, polygalacturonase 5g, proteolytic enzyme 5g, peptone 3g, glucose 3g, lactic acid 10ml, yeast extract paste 3g, red colouring agent for food, also used as a Chinese medicine 5g, yeast 5g, water is made into 200ml.
The enzyme promotor that is added in the multienzyme body mixed solution: NaCl 2g, CaCl
22g, KCl 2g, MgSO
47H
2O 0.5g.
Get 1 kilogram of Radix Glycyrrhizae in 50～60 ℃ of oven dry, be crushed to 20 orders, add pure water, regulate pH value 4.5～6.5 with lactic acid and soaked 8 hours, add pure water to medicinal material again and grab, decontrol and promptly scatter agglomerating on hand; Transfer PH to 4.0～5.0 to add 200ml multienzyme body mixed solution, stir, put in the enzymatic vessel 37 ℃ of enzymatic hydrolysis and fermentations 48 hours, stir oxygenation every day 3 times, carry out squeeze and filter after the end, filter residue filters merging filtrate two times with 50～60 ℃ of pure water washings, concentrate and be dried into powder, sterilising packaging is finished product.
Under the situation of identical Radix Glycyrrhizae consumption (1 kilogram), with above-mentioned finished product with traditional water boiling method and add the enzyme mixture method extract finished product measure the content (by the detection of Fujian Province Institute of Analysis) of glycyrrhizin respectively with high performance liquid chromatograph, the result is as shown in the table:
| The tradition poach | The mixture enzymolysis of enzyme | Bacterium oxalic acid multienzyme body mixed solution enzymolysis | |
| Glycyrrhizin (mg/g) | 350.5 | 480.6 | 525.0 |
| Extract gained paste quality (g) | 136.2 | 143.7 | ?200 |
Embodiment three:
The Radix Astragali: 1 kilogram.
Preparation multienzyme body mixed solution: cellulase 10g, polygalacturonase 5g, proteolytic enzyme 5g, peptone 3g, glucose 3g, lactic acid 10ml, yeast extract paste 3g, red colouring agent for food, also used as a Chinese medicine 5g, yeast 5g, water is made into 200ml.
The enzyme promotor that is added in the multienzyme body mixed solution: NaCl 2g, CaCl
22g, KCl 2g, MgSO
47H
2O 0.5g.
Get 1 kilogram of Radix Astragali in 50～60 ℃ of oven dry, be crushed to 20 orders, add 3 kilograms of pure water, regulate pH value 4.5～6.5 with lactic acid and soaked 8 hours, add pure water to medicinal material again and grab, decontrol and promptly scatter agglomerating on hand; Transfer PH to 4.0～5.0 to add multienzyme body mixed solutions, stir, 37 ℃ of enzymatic hydrolysis and fermentations are 48 hours to the enzymatic vessel, stir oxygenation every day 3 times, carry out squeeze and filter after the end, filter residue filters for two times with 50～60 ℃ of pure water washings, merging filtrate concentrates and is dried into powder, and sterilising packaging is finished product.
Under the situation of identical Radix Astragali consumption (1 kilogram), with above-mentioned finished product with traditional water boiling method and add the enzyme mixture method extract finished product use the content (by the detection of Fujian Province Institute of Analysis) of ultraviolet spectrophotometry astragalus polysaccharides respectively, the result is as shown in the table:
| The tradition poach | The mixture enzymolysis of enzyme | Bacterium oxalic acid multienzyme body mixed solution enzymolysis | |
| Crude polysaccharides content (mg/g) | 375.8 | 411.7 | 424.3 |
| Extract gained paste quality (g) | 89.5 | 250 | 400 |
The herbal medicine good effect that the present invention makes, pollution-free, application convenience, having the quick-acting advantage of Western medicine concurrently does not but have its toxicity and side effect, and the pharmacy cost is low, has bigger application and popularization value.
Claims (7)
1. method of utilizing the enzyme preparation herbal medicine is characterized in that this method follows these steps to carry out: Chinese medicinal materials is added pure water, grab agglomerating on hand with medicinal material, decontroling promptly scatters is principle; Add weak acid and regulate pH to 4.5～6.5, soak after 8～12 hours, add pure water again and make Chinese medicinal materials become pasty state, adjust pH 4.0～5.0; Put in the enzymatic vessel by the multienzyme body mixed solution that adds 200～250mL in the dried medicinal material of per kilogram, under 30～50 ℃, carry out enzymolysis, fermentation 48～72h, stir oxygenation once every 5～10h; Enzymatic hydrolysis and fermentation carries out squeeze and filter after finishing, and washs some times and filters, and merging filtrate separates purification, and packaging sterilizing gets product.
2. the method for utilizing the enzyme preparation herbal medicine according to claim 1 is characterized in that described multienzyme body mixed solution is made of the multienzyme body and the primary liquid that contain polygalacturonase, α-Dian Fenmei and proteolytic enzyme based on bacterium cellulose oxalate enzyme, simultaneously.
3. the method for utilizing the enzyme preparation herbal medicine according to claim 1 and 2 is characterized in that according to the prescription of the different multienzyme body of different medicinal material preparations being:
(1) for the Chinese medicinal materials of taking from root, stem, its multienzyme body prescription is: cellulase 1～2%, polygalacturonase 0.2～0.8%, α-Dian Fenmei 0.2～1.0%, proteinase-10 .2～0.8%;
(2) for the Chinese medicinal materials of taking from flower, fruit, its multienzyme body prescription is: cellulase 0.5～1.5%, polygalacturonase 0.5～1.5%, proteinase-10 .5～1.0%;
(3) for the Chinese medicinal materials of taking from leaf, herb, its multienzyme body prescription is: cellulase 0.5～1.5%, polygalacturonase are 0.2～0.8%, α-Dian Fenmei 0.2～0.8%, proteinase-10 .2～0.8%.
4. the method for utilizing the enzyme preparation herbal medicine according to claim 1 and 2 is characterized in that described primary liquid formula is: red colouring agent for food, also used as a Chinese medicine 0.5～1%, yeast 0.5～1%, peptone 0.2～0.3%, glucose 0.2～0.3%, lactic acid 1～3%, yeast extract paste 0.2～0.3%.
5. the method for utilizing the enzyme preparation herbal medicine according to claim 1 is characterized in that can adding an amount of inorganic salts pharmaceutical chemicals as enzyme promotor in described multienzyme body mixed solution.
6. the method for utilizing the enzyme preparation herbal medicine according to claim 6 is characterized in that described inorganic salts pharmaceutical chemicals is NaCl 0.2～0.5%, CaCl2 0.2～0.5%, KCl 0.2～0.5%, MgSO40.05～0.1%.
7. the method for utilizing the enzyme preparation herbal medicine according to claim 1 is characterized in that cleaning earlier before described Chinese medicinal materials uses, and removes the impurity and the medicinal material that goes mouldy, and after oven dry under 50～60 ℃, is crushed to 20～40 orders.
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| CN2005102003753A CN1891239B (en) | 2005-07-07 | 2005-07-07 | Method for preparing Chinese herb medicine utilizing enzyme preparation |
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| CN2005102003753A CN1891239B (en) | 2005-07-07 | 2005-07-07 | Method for preparing Chinese herb medicine utilizing enzyme preparation |
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| CN1891239B true CN1891239B (en) | 2011-05-18 |
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| CN101112419B (en) * | 2007-08-24 | 2010-12-08 | 王肇生 | Extracting technics of antineoplastic Chinese patent drug |
| CN103416629B (en) * | 2013-08-22 | 2014-06-11 | 广南(湛江)家丰饲料有限公司 | Pig feed additive capable of effectively improving pig immunity and promoting pig growth |
| CN104784233A (en) * | 2015-04-16 | 2015-07-22 | 珲春绿源参业生物科技有限公司 | Method for preparing panaxoside and panaxynol through enzymatic zymotechnics |
| CN107412275A (en) * | 2017-05-11 | 2017-12-01 | 张新 | The preparation method of a variety of biology enzyme micro mist fermentation preparations |
| CN110051703A (en) * | 2018-01-18 | 2019-07-26 | 任莉慧 | Quantum Chinese medicine and process of preparing |
| JP2019214543A (en) * | 2018-06-14 | 2019-12-19 | 科技研株式会社 | Crude drug active principle natural extraction method by living matter catalytic activity of plants |
| CN109370813B (en) * | 2018-11-16 | 2020-11-10 | 欧新永 | Raw material of washing and caring product and preparation method thereof |
| CN110195018A (en) * | 2019-06-21 | 2019-09-03 | 寿碧霞 | A method of utilizing more kinds of enzyme enzymatic hydrolysis animals and plants of N+1 and microorganism wall |
| CN110656130B (en) * | 2019-11-15 | 2021-05-11 | 滨州医学院 | Astragalus membranaceus-paecilomyces cicadae fermentation mycoplasm, preparation method and application |
| CN114617819B (en) * | 2022-04-25 | 2023-06-09 | 广州善合化工有限公司 | Purslane extract and preparation method and application thereof |
| CN115584565B (en) * | 2022-07-22 | 2023-08-18 | 新乡化纤股份有限公司 | Mushroom spinning slurry and preparation method and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1416843A (en) * | 2001-11-03 | 2003-05-14 | 福州济安堂菌草酸酶技术开发有限公司 | Enzyme prepn method of preparing Chinese medicine |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1416843A (en) * | 2001-11-03 | 2003-05-14 | 福州济安堂菌草酸酶技术开发有限公司 | Enzyme prepn method of preparing Chinese medicine |
Non-Patent Citations (1)
| Title |
|---|
| 韩丽等.酶技术在中药制剂中的应用.《中南药学》.2003,第1卷(第3期),157-159. * |
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