JP2014519832A5 - - Google Patents
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- JP2014519832A5 JP2014519832A5 JP2014516354A JP2014516354A JP2014519832A5 JP 2014519832 A5 JP2014519832 A5 JP 2014519832A5 JP 2014516354 A JP2014516354 A JP 2014516354A JP 2014516354 A JP2014516354 A JP 2014516354A JP 2014519832 A5 JP2014519832 A5 JP 2014519832A5
- Authority
- JP
- Japan
- Prior art keywords
- range
- chir
- cells
- concentration
- activin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 claims 12
- 241000766026 Coregonus nasus Species 0.000 claims 9
- 210000004039 endoderm cell Anatomy 0.000 claims 6
- 108010023082 activin A Proteins 0.000 claims 4
- 210000000130 stem cell Anatomy 0.000 claims 4
- 210000001671 embryonic stem cell Anatomy 0.000 claims 3
- 238000011534 incubation Methods 0.000 claims 3
- 239000002609 medium Substances 0.000 claims 3
- 239000003814 drug Substances 0.000 claims 2
- 229940079593 drug Drugs 0.000 claims 2
- 239000001963 growth medium Substances 0.000 claims 2
- 230000000638 stimulation Effects 0.000 claims 2
- 239000012980 RPMI-1640 medium Substances 0.000 claims 1
- 210000004027 cell Anatomy 0.000 claims 1
- 210000001900 endoderm Anatomy 0.000 claims 1
- 210000004263 induced pluripotent stem cell Anatomy 0.000 claims 1
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP11170713.9 | 2011-06-21 | ||
| EP11170713 | 2011-06-21 | ||
| US201161501351P | 2011-06-27 | 2011-06-27 | |
| US61/501,351 | 2011-06-27 | ||
| PCT/EP2012/062013 WO2012175633A1 (en) | 2011-06-21 | 2012-06-21 | Efficient induction of definitive endoderm from pluripotent stem cells |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2014519832A JP2014519832A (ja) | 2014-08-21 |
| JP2014519832A5 true JP2014519832A5 (enExample) | 2015-08-06 |
| JP6312591B2 JP6312591B2 (ja) | 2018-04-18 |
Family
ID=47422055
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2014516354A Active JP6312591B2 (ja) | 2011-06-21 | 2012-06-21 | 多能性幹細胞からの胚体内胚葉の効率的な誘導 |
Country Status (6)
| Country | Link |
|---|---|
| US (3) | US20140234963A1 (enExample) |
| EP (1) | EP2723852B1 (enExample) |
| JP (1) | JP6312591B2 (enExample) |
| CN (2) | CN108220224A (enExample) |
| ES (1) | ES2902650T3 (enExample) |
| WO (1) | WO2012175633A1 (enExample) |
Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP6312591B2 (ja) | 2011-06-21 | 2018-04-18 | ノヴォ ノルディスク アー/エス | 多能性幹細胞からの胚体内胚葉の効率的な誘導 |
| US20150247123A1 (en) | 2012-09-03 | 2015-09-03 | Novo Nordisk A/S | Generation of pancreatic endoderm from Pluripotent Stem cells using small molecules |
| GB201317869D0 (en) * | 2013-10-09 | 2013-11-20 | Cambridge Entpr Ltd | In vitro production of foregut stem cells |
| JP7123802B2 (ja) | 2016-02-24 | 2022-08-23 | ノヴォ ノルディスク アー/エス | ヒト多能性幹細胞由来内分泌前駆細胞からの機能的ベータ細胞の産生 |
| US20190071645A1 (en) * | 2017-09-07 | 2019-03-07 | Sergio Mora | Efficient differentiation of human stem cells to definitive endoderm |
| JP7389020B2 (ja) | 2017-09-11 | 2023-11-29 | ノヴォ ノルディスク アー/エス | 幹細胞からインビトロで誘導されたnkx6.1およびc-ペプチド共発現細胞の濃縮 |
| EP3790956A4 (en) | 2018-05-11 | 2022-03-16 | Cornell University | METHOD FOR INDUCING DIFFERENTIATION OF HUMAN PLURIPOTENT STEM CELLS INTO MONOHORMONAL CELLS |
| WO2020043292A1 (en) | 2018-08-30 | 2020-03-05 | Novo Nordisk A/S | Generation of functional beta cells from human pluripotent stem cell-derived endocrine progenitors |
| WO2020207998A1 (en) | 2019-04-08 | 2020-10-15 | Novo Nordisk A/S | Generation of pancreatic endoderm from stem cell derived definitive endoderm |
| US20220389378A1 (en) | 2019-11-22 | 2022-12-08 | Novo Nordisk A/S | Spin-aggregated neural microspheres and the application thereof |
| WO2022043518A1 (en) | 2020-08-28 | 2022-03-03 | Novo Nordisk A/S | Method for screening in vitro population of stem cell derived beta like cells and novel markers thereof |
| JP2025520898A (ja) | 2022-07-06 | 2025-07-03 | ノヴォ ノルディスク アー/エス | 幹細胞の膵臓内分泌細胞への分化 |
| WO2024151541A1 (en) | 2023-01-09 | 2024-07-18 | Sana Biotechnology, Inc. | Type-1 diabetes autoimmune mouse |
| WO2024243236A2 (en) | 2023-05-22 | 2024-11-28 | Sana Biotechnology, Inc. | Methods of delivery of islet cells and related methods |
| WO2025093467A1 (en) | 2023-10-31 | 2025-05-08 | Novo Nordisk A/S | Methods for preventing unwanted cells arising during differentiation of human pluripotent stem cells |
| EP4491712A1 (en) | 2024-03-20 | 2025-01-15 | Novo Nordisk A/S | Method for detecting contaminant cells in a stem cell derived cell population |
Family Cites Families (55)
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| US5523226A (en) * | 1993-05-14 | 1996-06-04 | Biotechnology Research And Development Corp. | Transgenic swine compositions and methods |
| WO1999065897A1 (en) | 1998-06-19 | 1999-12-23 | Chiron Corporation | Inhibitors of glycogen synthase kinase 3 |
| WO2002092756A2 (en) | 2001-05-15 | 2002-11-21 | Rappaport Family Institute For Research In The Medical Sciences | Insulin producing cells derived from human embryonic stem cells |
| CN101240262A (zh) | 2001-12-07 | 2008-08-13 | 杰龙公司 | 源自人胚胎干细胞的胰岛细胞 |
| IL162663A0 (en) | 2001-12-28 | 2005-11-20 | Cellartis Ab | A method for the establishment of apluripotent human blastocyst-derived stem cell line |
| US7625753B2 (en) | 2003-12-23 | 2009-12-01 | Cythera, Inc. | Expansion of definitive endoderm cells |
| MX2009009225A (es) * | 2003-12-23 | 2009-09-28 | Cythera Inc | Endodermo definitivo. |
| EP1740612B1 (en) | 2004-04-27 | 2019-08-07 | Viacyte, Inc. | Pdx1 expressing endoderm |
| AU2006301549B2 (en) | 2005-10-07 | 2013-02-14 | Cellartis Ab | A method for obtaining a xeno-free hBS cell line |
| SG170068A1 (en) | 2006-03-02 | 2011-04-29 | Cythera Inc | Endocrine precursor cells, pancreatic hormone-expressing cells and methods of production |
| GB0615327D0 (en) | 2006-03-30 | 2006-09-13 | Univ Edinburgh | Culture medium containing kinase inhibitors and uses thereof |
| ES2725601T3 (es) | 2006-04-28 | 2019-09-25 | Lifescan Inc | Diferenciación de células madre embriónicas humanas |
| US8741643B2 (en) * | 2006-04-28 | 2014-06-03 | Lifescan, Inc. | Differentiation of pluripotent stem cells to definitive endoderm lineage |
| AU2007254766A1 (en) | 2006-06-02 | 2007-12-13 | University Of Georgia Research Foundation, Inc. | Pancreatic and liver endoderm cells and tissue by differentiation of definitive endoderm cells obtained from human embryonic stems |
| CA2676044C (en) * | 2007-01-30 | 2019-10-22 | University Of Georgia Research Foundation, Inc. | Early mesoderm cells, a stable population of mesendoderm cells that has utility for generation of endoderm and mesoderm lineages and multipotent migratory cells (mmc) |
| EP2148569B1 (en) | 2007-04-23 | 2014-07-16 | Stowers Institute for Medical Research | Methods and compositions for hematopoeitic stem cell self-renewal |
| US9080145B2 (en) * | 2007-07-01 | 2015-07-14 | Lifescan Corporation | Single pluripotent stem cell culture |
| KR101555824B1 (ko) | 2007-07-18 | 2015-09-25 | 라이프스캔, 인코포레이티드 | 인간 배아 줄기 세포의 분화 |
| WO2009013254A1 (en) | 2007-07-20 | 2009-01-29 | Cellartis Ab | A novel population of hepatocytes derived via definitive endoderm (de-hep) from human blastocysts stem cells |
| MX2010001335A (es) * | 2007-07-31 | 2010-06-02 | Lifescan Inc | Diferenciacion de celulas madre embrionarias humanas. |
| EP2185691B1 (en) | 2007-07-31 | 2018-03-14 | Lifescan, Inc. | Pluripotent stem cell differentiation by using human feeder cells |
| EP2195417A1 (en) * | 2007-08-24 | 2010-06-16 | University Court of the University of Edinburgh | Regionalised endoderm cells and uses thereof |
| US8623648B2 (en) | 2008-04-24 | 2014-01-07 | Janssen Biotech, Inc. | Treatment of pluripotent cells |
| US7939322B2 (en) | 2008-04-24 | 2011-05-10 | Centocor Ortho Biotech Inc. | Cells expressing pluripotency markers and expressing markers characteristic of the definitive endoderm |
| CA2723820A1 (en) | 2008-05-09 | 2009-11-12 | Vistagen Therapeutics, Inc. | Pancreatic endocrine progenitor cells derived from pluripotent stem cells |
| DK2993226T3 (da) * | 2008-06-03 | 2021-02-22 | Viacyte Inc | Vækstfaktorer til fremstilling af en definitiv endoderm |
| US20090298178A1 (en) * | 2008-06-03 | 2009-12-03 | D Amour Kevin Allen | Growth factors for production of definitive endoderm |
| US20110091971A1 (en) | 2008-06-30 | 2011-04-21 | Janet Davis | Differentiation of Pluripotent Stem Cells |
| MX2011000125A (es) | 2008-06-30 | 2011-02-25 | Centocor Ortho Biotech Inc | Diferenciacion de celulas madre pluripotentes. |
| US20100028307A1 (en) | 2008-07-31 | 2010-02-04 | O'neil John J | Pluripotent stem cell differentiation |
| US8278105B2 (en) * | 2008-09-09 | 2012-10-02 | University Of Southern California | Induction, propagation and isolation of liver progenitor cells |
| CN101684454B (zh) * | 2008-09-25 | 2012-08-15 | 中国科学院上海生命科学研究院 | 一种定型内胚层细胞的制备和分离方法 |
| ES2727950T3 (es) | 2008-10-31 | 2019-10-21 | Janssen Biotech Inc | Diferenciación de células madre embrionarias humanas en linaje endocrino pancreático |
| PL2346988T3 (pl) | 2008-10-31 | 2017-10-31 | Janssen Biotech Inc | Różnicowanie ludzkich zarodkowych komórek macierzystych do linii endokrynnej trzustki |
| JP2012508584A (ja) | 2008-11-14 | 2012-04-12 | ヴィアサイト,インコーポレイテッド | ヒト多能性幹細胞由来膵臓細胞のカプセル化 |
| WO2010091241A2 (en) | 2009-02-06 | 2010-08-12 | President And Fellows Of Harvard College | Compositions and methods for promoting the generation of definitive endoderm |
| EP3904505A1 (en) | 2009-04-22 | 2021-11-03 | Viacyte, Inc. | Cell compositions derived from dedifferentiated reprogrammed cells |
| CN102812122A (zh) | 2009-05-27 | 2012-12-05 | 索尔克生物学研究院 | 基因矫正的无疾病的诱导的多能干细胞的产生 |
| SG177481A1 (en) | 2009-07-20 | 2012-02-28 | Janssen Biotech Inc | Differentiation of human embryonic stem cells |
| WO2011011302A2 (en) | 2009-07-20 | 2011-01-27 | Centocor Ortho Biotech Inc. | Differentiation of human embryonic stem cells |
| CA2784415C (en) | 2009-12-23 | 2019-06-18 | Jean Xu | Differentiation of human embryonic stem cells |
| KR101764404B1 (ko) | 2009-12-23 | 2017-08-03 | 얀센 바이오테크 인코포레이티드 | 인간 배아 줄기 세포의 분화 |
| JP5762979B2 (ja) | 2009-12-29 | 2015-08-12 | 武田薬品工業株式会社 | 膵ホルモン産生細胞の製造法 |
| PH12012501686A1 (en) | 2010-03-01 | 2020-10-19 | Janssen Biotech Inc | Methods for purifying cells derived from pluripotent stem cells |
| US9719068B2 (en) | 2010-05-06 | 2017-08-01 | Children's Hospital Medical Center | Methods and systems for converting precursor cells into intestinal tissues through directed differentiation |
| JP6050225B2 (ja) | 2010-05-12 | 2016-12-21 | ヤンセン バイオテツク,インコーポレーテツド | ヒト胚性幹細胞の分化 |
| US9085757B2 (en) | 2010-06-17 | 2015-07-21 | Regents Of The University Of Minnesota | Production of insulin producing cells |
| AU2011289379A1 (en) | 2010-08-12 | 2013-02-07 | Janssen Biotech, Inc. | Treatment of diabetes with pancreatic endocrine precursor cells |
| EP3372672A1 (en) | 2010-08-31 | 2018-09-12 | Janssen Biotech, Inc. | Differentiation of human embryonic stem cells |
| MX355077B (es) | 2010-08-31 | 2018-04-03 | Janssen Biotech Inc | Diferenciacion de celulas madre embrionarias humanas. |
| US9062292B2 (en) | 2010-09-13 | 2015-06-23 | Enzo Life Sciences Inc. | Mutant T7 polymerases |
| WO2012070014A2 (en) | 2010-11-26 | 2012-05-31 | Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. | Identification of novel cell surface markers for pancreatic progenitor cells and definite endodermal cells |
| AU2010365057B2 (en) | 2010-12-08 | 2016-12-22 | Viacyte, Inc. | Agents and methods for inhibiting human pluripotent stem cell growth |
| JP5922147B2 (ja) | 2010-12-15 | 2016-05-24 | カディマステム リミテッド | 多能性幹細胞に由来するインスリン産生細胞 |
| JP6312591B2 (ja) | 2011-06-21 | 2018-04-18 | ノヴォ ノルディスク アー/エス | 多能性幹細胞からの胚体内胚葉の効率的な誘導 |
-
2012
- 2012-06-21 JP JP2014516354A patent/JP6312591B2/ja active Active
- 2012-06-21 CN CN201810066827.0A patent/CN108220224A/zh active Pending
- 2012-06-21 US US14/127,296 patent/US20140234963A1/en not_active Abandoned
- 2012-06-21 CN CN201280040881.9A patent/CN103890167A/zh active Pending
- 2012-06-21 EP EP12735227.6A patent/EP2723852B1/en active Active
- 2012-06-21 WO PCT/EP2012/062013 patent/WO2012175633A1/en not_active Ceased
- 2012-06-21 ES ES12735227T patent/ES2902650T3/es active Active
-
2017
- 2017-09-15 US US15/705,705 patent/US10487313B2/en active Active
-
2019
- 2019-11-25 US US16/694,040 patent/US20200190477A1/en not_active Abandoned
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