JP2014502510A - 分子核酸ベースの技術を使用している細胞生存度を決定する改良された方法 - Google Patents

分子核酸ベースの技術を使用している細胞生存度を決定する改良された方法 Download PDF

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JP2014502510A
JP2014502510A JP2013547602A JP2013547602A JP2014502510A JP 2014502510 A JP2014502510 A JP 2014502510A JP 2013547602 A JP2013547602 A JP 2013547602A JP 2013547602 A JP2013547602 A JP 2013547602A JP 2014502510 A JP2014502510 A JP 2014502510A
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cells
pcr
dna
present
dead
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JP2014502510A5 (enExample
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マーク オ’ハラ、シャウン
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ゼウス サイエンティフィック、インク.
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/106Pharmacogenomics, i.e. genetic variability in individual responses to drugs and drug metabolism

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
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  • Immunology (AREA)
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  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
JP2013547602A 2010-12-31 2011-12-27 分子核酸ベースの技術を使用している細胞生存度を決定する改良された方法 Pending JP2014502510A (ja)

Applications Claiming Priority (3)

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US201061428892P 2010-12-31 2010-12-31
US61/428,892 2010-12-31
PCT/US2011/067329 WO2012092238A1 (en) 2010-12-31 2011-12-27 Improved methods for determining cell viability using molecular nucleic acid-based techniques

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JP2016110520A Division JP2016192967A (ja) 2010-12-31 2016-06-01 血液試料の微生物特異的フィルタ・インサイチュ分析の方法

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JP2014502510A true JP2014502510A (ja) 2014-02-03
JP2014502510A5 JP2014502510A5 (enExample) 2016-07-28

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JP2013547602A Pending JP2014502510A (ja) 2010-12-31 2011-12-27 分子核酸ベースの技術を使用している細胞生存度を決定する改良された方法
JP2016110520A Pending JP2016192967A (ja) 2010-12-31 2016-06-01 血液試料の微生物特異的フィルタ・インサイチュ分析の方法

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US (1) US20140186828A1 (enExample)
EP (1) EP2659001A4 (enExample)
JP (2) JP2014502510A (enExample)
CN (1) CN103476945A (enExample)
AU (2) AU2011352333B2 (enExample)
CA (1) CA2862523A1 (enExample)
NZ (1) NZ613671A (enExample)
WO (1) WO2012092238A1 (enExample)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2015513921A (ja) * 2012-04-12 2015-05-18 ゼウス サイエンティフィック、インク. 任意のポリメラーゼ伸長活性の高感度定量的測定と生細胞存在の決定とに有用なポリメラーゼ活性測定方法
US10495555B2 (en) * 2013-10-08 2019-12-03 David Putnam Filter-cartridge based fluid-sample preparation and assay system
US10112194B2 (en) 2014-04-14 2018-10-30 Q-Linea Ab Detection of microscopic objects
GB201617713D0 (en) * 2016-10-19 2016-11-30 Q-Linea Ab Method for recovering microbial cells
ES3030837T3 (en) * 2017-08-24 2025-07-02 Roche Diagnostics Gmbh Electrochemical detection of bacterial and/or fungal infections
US11319582B2 (en) * 2019-07-19 2022-05-03 Pathogendx, Inc. Methods for microbial DNA analysis
DE102020103971B4 (de) * 2020-02-14 2022-02-24 Testo bioAnalytics GmbH Verfahren zum Nachweis von lebenden Mikroorganismen und ein fluidisches Kanalsystem

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6210881B1 (en) * 1996-12-30 2001-04-03 Becton, Dickinson And Company Method for reducing inhibitors of nucleic acid hybridization
US20040248148A1 (en) * 2002-11-26 2004-12-09 Horgen Paul A. Ultrasensitive detection of pathogenic microbes
WO2007100762A2 (en) * 2006-02-28 2007-09-07 Montana State University Use of phenanthridium derivatives for distinguishing between intact and membrane-compromised cells using molecular nucleic acid-based techniques
WO2009071892A2 (en) * 2007-12-05 2009-06-11 Immunosolv Limited Method for the detection of dead and dying cells

Family Cites Families (12)

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WO2002052034A1 (fr) * 2000-12-26 2002-07-04 Joji Oshima Methodes de bioscopie et d'amplification d'acides nucleiques
AU2002354838B2 (en) * 2001-07-19 2007-11-29 Infectio Diagnostic (I.D.I.) Inc. Universal method and composition for the rapid lysis of cells for the release of nucleic acids and their detection
WO2004007547A2 (en) * 2002-07-12 2004-01-22 Axon Neuroscience Forschungs- Und Entwicklungs Gmbh Truncated tau proteins
US20050160528A1 (en) * 2003-07-28 2005-07-28 Clark Steven F. Bed frame with integral air purifying device
US7759348B2 (en) * 2003-07-30 2010-07-20 Xenon Pharmaceuticals Inc. Pyridazine derivatives and their use as therapeutic agents
FR2885140B1 (fr) * 2005-04-29 2010-12-31 Millipore Corp Procede de detection et de caracterisation de microorgarnismes sur une membrane.
JP2008125457A (ja) * 2006-11-22 2008-06-05 Asahi Breweries Ltd 微生物の検出方法
JP5245379B2 (ja) * 2007-12-04 2013-07-24 株式会社日立プラントテクノロジー 捕集デバイス、及びそれを用いる分析システム
EP2238258A4 (en) * 2007-12-24 2011-01-12 Zeus Scientific Inc METHOD AND COMPOSITIONS INCLUDING DIAGNOSTIC KITS FOR THE DETECTION OF STAPHYLOCOCCUS AUREUS
JP2009159868A (ja) * 2007-12-28 2009-07-23 Institute Of Physical & Chemical Research 造血幹細胞の維持・増幅方法
DK2262520T3 (en) * 2008-02-26 2017-08-07 Univ Cornell COMPOSITIONS FOR PREVENTION AND TREATMENT OF Kidney Injury
WO2009125804A1 (ja) * 2008-04-09 2009-10-15 武田薬品工業株式会社 スクリーニング方法

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6210881B1 (en) * 1996-12-30 2001-04-03 Becton, Dickinson And Company Method for reducing inhibitors of nucleic acid hybridization
US20040248148A1 (en) * 2002-11-26 2004-12-09 Horgen Paul A. Ultrasensitive detection of pathogenic microbes
WO2007100762A2 (en) * 2006-02-28 2007-09-07 Montana State University Use of phenanthridium derivatives for distinguishing between intact and membrane-compromised cells using molecular nucleic acid-based techniques
WO2009071892A2 (en) * 2007-12-05 2009-06-11 Immunosolv Limited Method for the detection of dead and dying cells

Also Published As

Publication number Publication date
NZ613671A (en) 2017-01-27
CN103476945A (zh) 2013-12-25
EP2659001A4 (en) 2014-07-02
AU2017201390A1 (en) 2017-03-23
US20140186828A1 (en) 2014-07-03
AU2011352333B2 (en) 2016-12-08
EP2659001A1 (en) 2013-11-06
AU2011352333A1 (en) 2013-08-15
WO2012092238A1 (en) 2012-07-05
JP2016192967A (ja) 2016-11-17
CA2862523A1 (en) 2012-07-05

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