CA2862523A1 - Improved methods for determining cell viability using molecular nucleic acid-based techniques - Google Patents

Improved methods for determining cell viability using molecular nucleic acid-based techniques Download PDF

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Publication number
CA2862523A1
CA2862523A1 CA2862523A CA2862523A CA2862523A1 CA 2862523 A1 CA2862523 A1 CA 2862523A1 CA 2862523 A CA2862523 A CA 2862523A CA 2862523 A CA2862523 A CA 2862523A CA 2862523 A1 CA2862523 A1 CA 2862523A1
Authority
CA
Canada
Prior art keywords
cells
pcr
dna
microbe
mixture
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA2862523A
Other languages
English (en)
French (fr)
Inventor
Shawn Mark O'hara
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Momentum Bioscience Ltd
Original Assignee
Zeus Scientific Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Zeus Scientific Inc filed Critical Zeus Scientific Inc
Publication of CA2862523A1 publication Critical patent/CA2862523A1/en
Abandoned legal-status Critical Current

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/106Pharmacogenomics, i.e. genetic variability in individual responses to drugs and drug metabolism

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
CA2862523A 2010-12-31 2011-12-27 Improved methods for determining cell viability using molecular nucleic acid-based techniques Abandoned CA2862523A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201061428892P 2010-12-31 2010-12-31
US61/428,892 2010-12-31
PCT/US2011/067329 WO2012092238A1 (en) 2010-12-31 2011-12-27 Improved methods for determining cell viability using molecular nucleic acid-based techniques

Publications (1)

Publication Number Publication Date
CA2862523A1 true CA2862523A1 (en) 2012-07-05

Family

ID=46383494

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2862523A Abandoned CA2862523A1 (en) 2010-12-31 2011-12-27 Improved methods for determining cell viability using molecular nucleic acid-based techniques

Country Status (8)

Country Link
US (1) US20140186828A1 (enExample)
EP (1) EP2659001A4 (enExample)
JP (2) JP2014502510A (enExample)
CN (1) CN103476945A (enExample)
AU (2) AU2011352333B2 (enExample)
CA (1) CA2862523A1 (enExample)
NZ (1) NZ613671A (enExample)
WO (1) WO2012092238A1 (enExample)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2015513921A (ja) * 2012-04-12 2015-05-18 ゼウス サイエンティフィック、インク. 任意のポリメラーゼ伸長活性の高感度定量的測定と生細胞存在の決定とに有用なポリメラーゼ活性測定方法
US10495555B2 (en) * 2013-10-08 2019-12-03 David Putnam Filter-cartridge based fluid-sample preparation and assay system
US10112194B2 (en) 2014-04-14 2018-10-30 Q-Linea Ab Detection of microscopic objects
GB201617713D0 (en) * 2016-10-19 2016-11-30 Q-Linea Ab Method for recovering microbial cells
ES3030837T3 (en) * 2017-08-24 2025-07-02 Roche Diagnostics Gmbh Electrochemical detection of bacterial and/or fungal infections
US11319582B2 (en) * 2019-07-19 2022-05-03 Pathogendx, Inc. Methods for microbial DNA analysis
DE102020103971B4 (de) * 2020-02-14 2022-02-24 Testo bioAnalytics GmbH Verfahren zum Nachweis von lebenden Mikroorganismen und ein fluidisches Kanalsystem

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6210881B1 (en) * 1996-12-30 2001-04-03 Becton, Dickinson And Company Method for reducing inhibitors of nucleic acid hybridization
WO2002052034A1 (fr) * 2000-12-26 2002-07-04 Joji Oshima Methodes de bioscopie et d'amplification d'acides nucleiques
AU2002354838B2 (en) * 2001-07-19 2007-11-29 Infectio Diagnostic (I.D.I.) Inc. Universal method and composition for the rapid lysis of cells for the release of nucleic acids and their detection
WO2004007547A2 (en) * 2002-07-12 2004-01-22 Axon Neuroscience Forschungs- Und Entwicklungs Gmbh Truncated tau proteins
US20040248148A1 (en) * 2002-11-26 2004-12-09 Horgen Paul A. Ultrasensitive detection of pathogenic microbes
US20050160528A1 (en) * 2003-07-28 2005-07-28 Clark Steven F. Bed frame with integral air purifying device
US7759348B2 (en) * 2003-07-30 2010-07-20 Xenon Pharmaceuticals Inc. Pyridazine derivatives and their use as therapeutic agents
FR2885140B1 (fr) * 2005-04-29 2010-12-31 Millipore Corp Procede de detection et de caracterisation de microorgarnismes sur une membrane.
PL2004843T3 (pl) * 2006-02-28 2015-12-31 Univ Montana State Zastosowanie pochodnych fenantrydyny dla rozróżniania komórek nienaruszonych i komórek z uszkodzoną błoną za pomocą technik molekularnych opartych o kwas nukleinowy
JP2008125457A (ja) * 2006-11-22 2008-06-05 Asahi Breweries Ltd 微生物の検出方法
JP5245379B2 (ja) * 2007-12-04 2013-07-24 株式会社日立プラントテクノロジー 捕集デバイス、及びそれを用いる分析システム
GB0723797D0 (en) * 2007-12-05 2008-01-16 Immunosolv Ltd Method
EP2238258A4 (en) * 2007-12-24 2011-01-12 Zeus Scientific Inc METHOD AND COMPOSITIONS INCLUDING DIAGNOSTIC KITS FOR THE DETECTION OF STAPHYLOCOCCUS AUREUS
JP2009159868A (ja) * 2007-12-28 2009-07-23 Institute Of Physical & Chemical Research 造血幹細胞の維持・増幅方法
DK2262520T3 (en) * 2008-02-26 2017-08-07 Univ Cornell COMPOSITIONS FOR PREVENTION AND TREATMENT OF Kidney Injury
WO2009125804A1 (ja) * 2008-04-09 2009-10-15 武田薬品工業株式会社 スクリーニング方法

Also Published As

Publication number Publication date
NZ613671A (en) 2017-01-27
CN103476945A (zh) 2013-12-25
EP2659001A4 (en) 2014-07-02
AU2017201390A1 (en) 2017-03-23
US20140186828A1 (en) 2014-07-03
AU2011352333B2 (en) 2016-12-08
EP2659001A1 (en) 2013-11-06
AU2011352333A1 (en) 2013-08-15
WO2012092238A1 (en) 2012-07-05
JP2016192967A (ja) 2016-11-17
JP2014502510A (ja) 2014-02-03

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Legal Events

Date Code Title Description
EEER Examination request

Effective date: 20161005

FZDE Discontinued

Effective date: 20181227