CN103476945A - 使用基于分子核酸的技术确定细胞活性的改进方法 - Google Patents

使用基于分子核酸的技术确定细胞活性的改进方法 Download PDF

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Publication number
CN103476945A
CN103476945A CN2011800653159A CN201180065315A CN103476945A CN 103476945 A CN103476945 A CN 103476945A CN 2011800653159 A CN2011800653159 A CN 2011800653159A CN 201180065315 A CN201180065315 A CN 201180065315A CN 103476945 A CN103476945 A CN 103476945A
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cells
pcr
dna
dead
mixture
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Chinese (zh)
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肖恩·马克·奥哈拉
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Zeus Scientific Inc
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Zeus Scientific Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/106—Pharmacogenomics, i.e. genetic variability in individual responses to drugs and drug metabolism

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
CN2011800653159A 2010-12-31 2011-12-27 使用基于分子核酸的技术确定细胞活性的改进方法 Pending CN103476945A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201061428892P 2010-12-31 2010-12-31
US61/428,892 2010-12-31
PCT/US2011/067329 WO2012092238A1 (en) 2010-12-31 2011-12-27 Improved methods for determining cell viability using molecular nucleic acid-based techniques

Publications (1)

Publication Number Publication Date
CN103476945A true CN103476945A (zh) 2013-12-25

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Family Applications (1)

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CN2011800653159A Pending CN103476945A (zh) 2010-12-31 2011-12-27 使用基于分子核酸的技术确定细胞活性的改进方法

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Country Link
US (1) US20140186828A1 (enExample)
EP (1) EP2659001A4 (enExample)
JP (2) JP2014502510A (enExample)
CN (1) CN103476945A (enExample)
AU (2) AU2011352333B2 (enExample)
CA (1) CA2862523A1 (enExample)
NZ (1) NZ613671A (enExample)
WO (1) WO2012092238A1 (enExample)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109890976A (zh) * 2016-10-19 2019-06-14 Q-莱纳公司 回收微生物细胞的方法
CN113265445A (zh) * 2020-02-14 2021-08-17 泰斯托生物分析有限公司 用于检测活微生物的方法和流体管道系统
CN114787377A (zh) * 2019-07-19 2022-07-22 帕瑟根迪艾斯有限公司 微生物的dna分析方法

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* Cited by examiner, † Cited by third party
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KR20150009539A (ko) * 2012-04-12 2015-01-26 제우스 사이언티픽, 아이엔씨. 어떤 중합효소 연장 활성의 민감한, 정량적 측정에 유용한 중합효소 활성을 측정하고 생육성 세포의 존재를 결정하는 방법
US10495555B2 (en) * 2013-10-08 2019-12-03 David Putnam Filter-cartridge based fluid-sample preparation and assay system
US10112194B2 (en) 2014-04-14 2018-10-30 Q-Linea Ab Detection of microscopic objects
EP4549590A3 (en) * 2017-08-24 2025-08-06 Roche Diagnostics GmbH Electrochemical detection of bacterial and/or fungal infections

Citations (2)

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US6210881B1 (en) * 1996-12-30 2001-04-03 Becton, Dickinson And Company Method for reducing inhibitors of nucleic acid hybridization
WO2007100762A2 (en) * 2006-02-28 2007-09-07 Montana State University Use of phenanthridium derivatives for distinguishing between intact and membrane-compromised cells using molecular nucleic acid-based techniques

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JPWO2002052034A1 (ja) * 2000-12-26 2004-04-30 大島　譲二 生死判別方法及び核酸増幅方法
US7494771B2 (en) * 2001-07-19 2009-02-24 Geneohm Sciences Canada, Inc. Universal method and composition for the rapid lysis of cells for the release of nucleic acids and their detection
JP4414332B2 (ja) * 2002-07-12 2010-02-10 アクソン・ニューロサイエンス・フォルシュングス−ウント・エントヴィックルングス・ゲゼルシャフト・ミット・ベシュレンクテル・ハフツング アルツハイマーｔａｕタンパク質を発現するトランスジェニック動物
CA2448098A1 (en) * 2002-11-26 2004-05-26 Paul A. Horgen Ultrasensitive detection of pathogenic microbes
US20050160528A1 (en) * 2003-07-28 2005-07-28 Clark Steven F. Bed frame with integral air purifying device
US7759348B2 (en) * 2003-07-30 2010-07-20 Xenon Pharmaceuticals Inc. Pyridazine derivatives and their use as therapeutic agents
FR2885140B1 (fr) * 2005-04-29 2010-12-31 Millipore Corp Procede de detection et de caracterisation de microorgarnismes sur une membrane.
JP2008125457A (ja) * 2006-11-22 2008-06-05 Asahi Breweries Ltd 微生物の検出方法
JP5245379B2 (ja) * 2007-12-04 2013-07-24 株式会社日立プラントテクノロジー 捕集デバイス、及びそれを用いる分析システム
GB0723797D0 (en) * 2007-12-05 2008-01-16 Immunosolv Ltd Method
US20110111399A1 (en) * 2007-12-24 2011-05-12 O'hara Shawn Mark Methods And Compositions Including Diagnostic Kits For The Detection of Staphylococcus Aureus
JP2009159868A (ja) * 2007-12-28 2009-07-23 Institute Of Physical & Chemical Research 造血幹細胞の維持・増幅方法
JP5677096B2 (ja) * 2008-02-26 2015-02-25 コーネル ユニヴァーシティー 急性腎障害を予防および治療するための方法
WO2009125804A1 (ja) * 2008-04-09 2009-10-15 武田薬品工業株式会社 スクリーニング方法

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6210881B1 (en) * 1996-12-30 2001-04-03 Becton, Dickinson And Company Method for reducing inhibitors of nucleic acid hybridization
WO2007100762A2 (en) * 2006-02-28 2007-09-07 Montana State University Use of phenanthridium derivatives for distinguishing between intact and membrane-compromised cells using molecular nucleic acid-based techniques

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
EPHRAIM L. TSALIK ET AL.: "Multiplex PCR To Diagnose Bloodstream Infections in Patients Admitted from the Emergency Department with Sepsis", 《JOURNAL OF CLINICAL MICROBIOLOGY》 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109890976A (zh) * 2016-10-19 2019-06-14 Q-莱纳公司 回收微生物细胞的方法
CN114787377A (zh) * 2019-07-19 2022-07-22 帕瑟根迪艾斯有限公司 微生物的dna分析方法
EP3999656A4 (en) * 2019-07-19 2023-08-23 Pathogendx, Inc. METHODS FOR MICROBIAL DNA ANALYSIS
CN113265445A (zh) * 2020-02-14 2021-08-17 泰斯托生物分析有限公司 用于检测活微生物的方法和流体管道系统

Also Published As

Publication number Publication date
AU2011352333B2 (en) 2016-12-08
JP2016192967A (ja) 2016-11-17
US20140186828A1 (en) 2014-07-03
NZ613671A (en) 2017-01-27
AU2017201390A1 (en) 2017-03-23
WO2012092238A1 (en) 2012-07-05
CA2862523A1 (en) 2012-07-05
JP2014502510A (ja) 2014-02-03
EP2659001A4 (en) 2014-07-02
EP2659001A1 (en) 2013-11-06
AU2011352333A1 (en) 2013-08-15

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