JP2014118402A5 - - Google Patents
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- Publication number
- JP2014118402A5 JP2014118402A5 JP2012276858A JP2012276858A JP2014118402A5 JP 2014118402 A5 JP2014118402 A5 JP 2014118402A5 JP 2012276858 A JP2012276858 A JP 2012276858A JP 2012276858 A JP2012276858 A JP 2012276858A JP 2014118402 A5 JP2014118402 A5 JP 2014118402A5
- Authority
- JP
- Japan
- Prior art keywords
- purification
- protein
- chromatography
- purification method
- amino acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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- 238000000746 purification Methods 0.000 claims 10
- 150000001413 amino acids Chemical class 0.000 claims 6
- 238000000034 method Methods 0.000 claims 6
- 102000004169 proteins and genes Human genes 0.000 claims 5
- 108090000623 proteins and genes Proteins 0.000 claims 5
- 238000004587 chromatography analysis Methods 0.000 claims 3
- 102000014914 Carrier Proteins Human genes 0.000 claims 2
- 241000588724 Escherichia coli Species 0.000 claims 2
- KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 claims 2
- KZSNJWFQEVHDMF-UHFFFAOYSA-N Valine Natural products CC(C)C(N)C(O)=O KZSNJWFQEVHDMF-UHFFFAOYSA-N 0.000 claims 2
- 108091008324 binding proteins Proteins 0.000 claims 2
- 238000005277 cation exchange chromatography Methods 0.000 claims 2
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 claims 2
- 150000003242 quaternary ammonium salts Chemical class 0.000 claims 2
- 239000004474 valine Substances 0.000 claims 2
- LZZYPRNAOMGNLH-UHFFFAOYSA-M Cetrimonium bromide Chemical group [Br-].CCCCCCCCCCCCCCCC[N+](C)(C)C LZZYPRNAOMGNLH-UHFFFAOYSA-M 0.000 claims 1
- 125000002057 carboxymethyl group Chemical group [H]OC(=O)C([H])([H])[*] 0.000 claims 1
- 239000013604 expression vector Substances 0.000 claims 1
- 102000040430 polynucleotide Human genes 0.000 claims 1
- 108091033319 polynucleotide Proteins 0.000 claims 1
- 239000002157 polynucleotide Substances 0.000 claims 1
- 230000001131 transforming effect Effects 0.000 claims 1
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2012276858A JP6136236B2 (ja) | 2012-12-19 | 2012-12-19 | 組換えタンパク質の精製方法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2012276858A JP6136236B2 (ja) | 2012-12-19 | 2012-12-19 | 組換えタンパク質の精製方法 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2014118402A JP2014118402A (ja) | 2014-06-30 |
| JP2014118402A5 true JP2014118402A5 (enExample) | 2015-12-10 |
| JP6136236B2 JP6136236B2 (ja) | 2017-05-31 |
Family
ID=51173556
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2012276858A Active JP6136236B2 (ja) | 2012-12-19 | 2012-12-19 | 組換えタンパク質の精製方法 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP6136236B2 (enExample) |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3488513B2 (ja) * | 1994-07-07 | 2004-01-19 | サンスター株式会社 | エンドトキシンの吸着除去剤 |
| JP2000072659A (ja) * | 1998-08-25 | 2000-03-07 | Nippi:Kk | エンドトキシンの不活化法 |
| GB9927801D0 (en) * | 1999-11-24 | 2000-01-26 | Danisco | Method |
| US7125967B2 (en) * | 2003-10-08 | 2006-10-24 | Adjuvant Pharmaceuticals, Llc | Water-soluble chitosan having low endotoxin concentration and methods for making and using the same |
| US7767399B2 (en) * | 2005-01-31 | 2010-08-03 | Merck & Co., Inc. | Purification process for plasmid DNA |
| EA201071086A1 (ru) * | 2008-03-18 | 2011-04-29 | Новартис Аг | Усовершенствованный способ получения вакцинных антигенов вируса гриппа |
| JP5812627B2 (ja) * | 2010-03-10 | 2015-11-17 | 公益財団法人相模中央化学研究所 | 改良Ｆｃ受容体およびその製造方法 |
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2012
- 2012-12-19 JP JP2012276858A patent/JP6136236B2/ja active Active