JP2014076042A - 組換え細胞、並びに、β−フェランドレンの生産方法 - Google Patents

組換え細胞、並びに、β−フェランドレンの生産方法 Download PDF

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JP2014076042A
JP2014076042A JP2013119612A JP2013119612A JP2014076042A JP 2014076042 A JP2014076042 A JP 2014076042A JP 2013119612 A JP2013119612 A JP 2013119612A JP 2013119612 A JP2013119612 A JP 2013119612A JP 2014076042 A JP2014076042 A JP 2014076042A
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Prior art keywords
ferrandrene
synthase
nucleic acid
amino acid
acid sequence
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JP2013119612A
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Japanese (ja)
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JP2014076042A5 (enExample
Inventor
Masahiro Furuya
昌弘 古谷
Koichiro Iwasa
航一郎 岩佐
Tomoyuki Kori
悌之 郡
Shota Uenishi
章太 上西
Seiji Takahashi
征司 高橋
Takefumi Shimoyama
武文 下山
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Tohoku University NUC
Sekisui Chemical Co Ltd
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Tohoku University NUC
Sekisui Chemical Co Ltd
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Priority to JP2013119612A priority Critical patent/JP2014076042A/ja
Priority to EP13838825.1A priority patent/EP2899263A4/en
Priority to US14/429,510 priority patent/US9617563B2/en
Priority to KR1020157008333A priority patent/KR20150059649A/ko
Priority to CN201380060882.4A priority patent/CN104797704A/zh
Priority to PCT/JP2013/075282 priority patent/WO2014046174A1/ja
Publication of JP2014076042A publication Critical patent/JP2014076042A/ja
Publication of JP2014076042A5 publication Critical patent/JP2014076042A5/ja
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/007—Preparation of hydrocarbons or halogenated hydrocarbons containing one or more isoprene units, i.e. terpenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/002—Preparation of hydrocarbons or halogenated hydrocarbons cyclic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y205/00—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5)
    • C12Y205/01—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5) transferring alkyl or aryl groups, other than methyl groups (2.5.1)
    • C12Y205/01001—Dimethylallyltranstransferase (2.5.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y205/00—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5)
    • C12Y205/01—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5) transferring alkyl or aryl groups, other than methyl groups (2.5.1)
    • C12Y205/01028—Dimethylallylcistransferase (2.5.1.28)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/03—Carbon-oxygen lyases (4.2) acting on phosphates (4.2.3)
    • C12Y402/03051—Beta-phellandrene synthase (neryl-diphosphate-cyclizing) (4.2.3.51)

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
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  • Wood Science & Technology (AREA)
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  • General Engineering & Computer Science (AREA)
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  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2013119612A 2012-09-21 2013-06-06 組換え細胞、並びに、β−フェランドレンの生産方法 Pending JP2014076042A (ja)

Priority Applications (6)

Application Number Priority Date Filing Date Title
JP2013119612A JP2014076042A (ja) 2012-09-21 2013-06-06 組換え細胞、並びに、β−フェランドレンの生産方法
EP13838825.1A EP2899263A4 (en) 2012-09-21 2013-09-19 RECOMBINANT CELL AND METHOD FOR THE PRODUCTION OF BETA PHELLANDREN
US14/429,510 US9617563B2 (en) 2012-09-21 2013-09-19 Recombinant cell, and method for producing β-phellandrene
KR1020157008333A KR20150059649A (ko) 2012-09-21 2013-09-19 재조합 세포, 및 β-펠란드렌의 생산 방법
CN201380060882.4A CN104797704A (zh) 2012-09-21 2013-09-19 重组细胞以及β-水芹烯的生产方法
PCT/JP2013/075282 WO2014046174A1 (ja) 2012-09-21 2013-09-19 組換え細胞、並びに、β－フェランドレンの生産方法

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2012208292 2012-09-21
JP2012208292 2012-09-21
JP2013119612A JP2014076042A (ja) 2012-09-21 2013-06-06 組換え細胞、並びに、β−フェランドレンの生産方法

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JP2014076042A true JP2014076042A (ja) 2014-05-01
JP2014076042A5 JP2014076042A5 (enExample) 2016-06-30

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US (1) US9617563B2 (enExample)
EP (1) EP2899263A4 (enExample)
JP (1) JP2014076042A (enExample)
KR (1) KR20150059649A (enExample)
CN (1) CN104797704A (enExample)
WO (1) WO2014046174A1 (enExample)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2015081266A (ja) * 2013-10-21 2015-04-27 国立大学法人名古屋大学 βフェランドレン重合体、その製造方法及び成形品
WO2015060310A1 (ja) * 2013-10-21 2015-04-30 国立大学法人名古屋大学 βフェランドレン重合体、その製造方法及び成形品
WO2016129637A1 (ja) * 2015-02-13 2016-08-18 積水化学工業株式会社 核酸、融合タンパク質、組換え細胞、並びに、イソプレン又は環式テルペンの生産方法
JP2017106043A (ja) * 2017-03-21 2017-06-15 国立大学法人名古屋大学 βフェランドレン重合体、その製造方法及び成形品

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IN2014DN07103A (enExample) * 2012-02-06 2015-04-24 Univ California
JPWO2017094053A1 (ja) * 2015-11-30 2018-09-13 積水化学工業株式会社 組換え細胞、組換え細胞の製造方法、並びに、有機化合物の生産方法
CN107815424B (zh) * 2016-09-12 2021-03-05 华东理工大学 一种产柠檬烯的解脂耶氏酵母基因工程菌及其应用
CN109810999B (zh) * 2019-03-11 2023-03-24 湖北工业大学 一种利用微生物发酵生产橙花醇的方法
CN112257225B (zh) * 2020-09-16 2023-07-14 中国科学院地理科学与资源研究所 一种适用于高寒草地生态系统的npp计算方法
CN114350713A (zh) * 2021-12-30 2022-04-15 中国林业科学研究院亚热带林业研究所 山鸡椒水芹烯合成酶在制备萜烯类化合物及包含萜烯类化合物的产品中的应用

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JP2000245482A (ja) * 1999-03-05 2000-09-12 Sozoteki Seibutsu Kogaku Kenkyusho:Kk モノテルペンの生産方法及びそのための微生物
JP2005500805A (ja) * 2000-09-01 2005-01-13 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー 環式テルペノイド類の製造
JP2010539902A (ja) * 2007-09-20 2010-12-24 アミリス バイオテクノロジーズ，インコーポレイティド イソプレノイドの生産

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JP2000145482A (ja) * 1998-11-11 2000-05-26 Asmo Co Ltd スロットル制御装置
US6660507B2 (en) 2000-09-01 2003-12-09 E. I. Du Pont De Nemours And Company Genes involved in isoprenoid compound production
US7172886B2 (en) * 2001-12-06 2007-02-06 The Regents Of The University Of California Biosynthesis of isopentenyl pyrophosphate
NZ598302A (en) * 2007-09-11 2013-08-30 Sapphire Energy Inc Molecule production by photosynthetic organisms
IN2014DN07103A (enExample) 2012-02-06 2015-04-24 Univ California

Patent Citations (3)

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Publication number Priority date Publication date Assignee Title
JP2000245482A (ja) * 1999-03-05 2000-09-12 Sozoteki Seibutsu Kogaku Kenkyusho:Kk モノテルペンの生産方法及びそのための微生物
JP2005500805A (ja) * 2000-09-01 2005-01-13 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー 環式テルペノイド類の製造
JP2010539902A (ja) * 2007-09-20 2010-12-24 アミリス バイオテクノロジーズ，インコーポレイティド イソプレノイドの生産

Non-Patent Citations (6)

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Title
A. L. SCHILMILLER, ET AL: "Monoterpenes in the glandular trichomes of tomato are synthesized from a neryl diphosphate precursor", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, vol. 106, no. 26, JPN6013052232, 2009, pages 10865 - 10870, XP055244652, ISSN: 0003630529, DOI: 10.1073/pnas.0904113106 *
ARABIDOPSIS THALIANA MRNA FOR GERANYL DIPHOSPHATE SYNTHASE, JPN6013052234, ISSN: 0003630530 *
LAVANDULA ANGUSTIFOLIA CULTIVAR LADY BETA-PHELLANDRENE SYNTHASE MRNA, COMPLETE CDS, JPN6013052241, ISSN: 0003630533 *
SOLANUM LYCOPERSICUM CULTIVAR M82 NERYL DIPHOSPHATE SYNTHASE 1 (NDPS1) MRNA, COMPLETE CDS, JPN6013052237, ISSN: 0003630531 *
SOLANUM LYCOPERSICUM CULTIVAR M82 TERPENE SYNTHASE (PHS1) MRNA, COMPLETE CDS, JPN6013052240, ISSN: 0003630532 *
Z. A. DEMISSIE, ET AL: "Cloning and functional characterization of β-phellandrene synthase from Lavandula angustifolia", PLANTA, vol. 233, JPN6013052229, 2011, pages 685 - 696, XP055244651, ISSN: 0003630528, DOI: 10.1007/s00425-010-1332-5 *

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2015081266A (ja) * 2013-10-21 2015-04-27 国立大学法人名古屋大学 βフェランドレン重合体、その製造方法及び成形品
WO2015060310A1 (ja) * 2013-10-21 2015-04-30 国立大学法人名古屋大学 βフェランドレン重合体、その製造方法及び成形品
US10007029B2 (en) 2013-10-21 2018-06-26 National University Corporation Nagoya University β-phellandrene polymer, production method for same, and molded article
WO2016129637A1 (ja) * 2015-02-13 2016-08-18 積水化学工業株式会社 核酸、融合タンパク質、組換え細胞、並びに、イソプレン又は環式テルペンの生産方法
JP2017106043A (ja) * 2017-03-21 2017-06-15 国立大学法人名古屋大学 βフェランドレン重合体、その製造方法及び成形品

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CN104797704A (zh) 2015-07-22
US9617563B2 (en) 2017-04-11
EP2899263A4 (en) 2016-04-13
KR20150059649A (ko) 2015-06-01
EP2899263A1 (en) 2015-07-29
US20150218589A1 (en) 2015-08-06
WO2014046174A1 (ja) 2014-03-27

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