JP2014005231A5 - - Google Patents
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- Publication number
- JP2014005231A5 JP2014005231A5 JP2012141488A JP2012141488A JP2014005231A5 JP 2014005231 A5 JP2014005231 A5 JP 2014005231A5 JP 2012141488 A JP2012141488 A JP 2012141488A JP 2012141488 A JP2012141488 A JP 2012141488A JP 2014005231 A5 JP2014005231 A5 JP 2014005231A5
- Authority
- JP
- Japan
- Prior art keywords
- biological material
- solution
- fructose
- clarification
- clearing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000012620 biological material Substances 0.000 claims 19
- 239000000243 solution Substances 0.000 claims 15
- 238000000034 method Methods 0.000 claims 10
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 claims 9
- 229930091371 Fructose Natural products 0.000 claims 9
- 239000005715 Fructose Substances 0.000 claims 9
- 238000005352 clarification Methods 0.000 claims 6
- 239000003153 chemical reaction reagent Substances 0.000 claims 5
- 239000007864 aqueous solution Substances 0.000 claims 4
- 241001465754 Metazoa Species 0.000 claims 2
- 229920006395 saturated elastomer Polymers 0.000 claims 2
- 102000034287 fluorescent proteins Human genes 0.000 claims 1
- 108091006047 fluorescent proteins Proteins 0.000 claims 1
- 210000000056 organ Anatomy 0.000 claims 1
Priority Applications (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2012141488A JP5967528B2 (ja) | 2012-06-22 | 2012-06-22 | 生物材料を透明化する方法および生物材料用透明化処理キット |
| PCT/JP2013/067085 WO2013191274A1 (ja) | 2012-06-22 | 2013-06-21 | 生物材料を透明化する方法および生物材料用透明化処理キット |
| US14/408,647 US9933342B2 (en) | 2012-06-22 | 2013-06-21 | Method for rendering biological material transparent and processing kit for rendering biological material transparent |
| EP13806589.1A EP2866017B1 (en) | 2012-06-22 | 2013-06-21 | Method for rendering biological material transparent |
| US15/901,618 US10883903B2 (en) | 2012-06-22 | 2018-02-21 | Method for rendering biological material transparent and processing kit for rendering biological material transparent |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2012141488A JP5967528B2 (ja) | 2012-06-22 | 2012-06-22 | 生物材料を透明化する方法および生物材料用透明化処理キット |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2014005231A JP2014005231A (ja) | 2014-01-16 |
| JP2014005231A5 true JP2014005231A5 (enExample) | 2015-06-25 |
| JP5967528B2 JP5967528B2 (ja) | 2016-08-10 |
Family
ID=49768865
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2012141488A Active JP5967528B2 (ja) | 2012-06-22 | 2012-06-22 | 生物材料を透明化する方法および生物材料用透明化処理キット |
Country Status (4)
| Country | Link |
|---|---|
| US (2) | US9933342B2 (enExample) |
| EP (1) | EP2866017B1 (enExample) |
| JP (1) | JP5967528B2 (enExample) |
| WO (1) | WO2013191274A1 (enExample) |
Families Citing this family (34)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP6044900B2 (ja) | 2011-05-20 | 2016-12-14 | 国立研究開発法人理化学研究所 | 生物材料用透明化試薬、及びその利用 |
| EP2883030B1 (en) | 2012-08-09 | 2022-08-31 | The Board of Trustees of the Leland Stanford Junior University | Methods and compositions for preparing biological specimens for microscopic analysis |
| EP3108218A4 (en) * | 2014-02-21 | 2017-11-15 | Massachusetts Institute Of Technology | Expansion microscopy |
| AU2015266894A1 (en) | 2014-05-30 | 2016-08-25 | The Board Of Trustees Of The Leland Stanford Junior University | Methods and devices for imaging large intact tissue samples |
| EP3167272B8 (en) * | 2014-07-07 | 2022-03-23 | Chih-Yung Lin | Aqueous tissue clearing solution and uses thereof |
| EP3249382B1 (en) * | 2015-01-20 | 2022-03-02 | Riken | Biological-specimen transparentizing agent, system, and use therefor |
| US20180031452A1 (en) * | 2015-03-18 | 2018-02-01 | Riken | Method for observing biological material and clearing method |
| US11408890B2 (en) | 2015-04-14 | 2022-08-09 | Massachusetts Institute Of Technology | Iterative expansion microscopy |
| US10059990B2 (en) | 2015-04-14 | 2018-08-28 | Massachusetts Institute Of Technology | In situ nucleic acid sequencing of expanded biological samples |
| US10526649B2 (en) | 2015-04-14 | 2020-01-07 | Massachusetts Institute Of Technology | Augmenting in situ nucleic acid sequencing of expanded biological samples with in vitro sequence information |
| EP3332029B1 (en) | 2015-08-07 | 2021-10-06 | Massachusetts Institute of Technology | Nanoscale imaging of proteins and nucleic acids via expansion microscopy |
| US10317321B2 (en) | 2015-08-07 | 2019-06-11 | Massachusetts Institute Of Technology | Protein retention expansion microscopy |
| US20180348104A1 (en) * | 2015-12-01 | 2018-12-06 | Universitaet Duisburg-Essen | Non-hazardous optical clearing of biological samples |
| JP6601842B2 (ja) * | 2015-12-17 | 2019-11-06 | 国立大学法人名古屋大学 | 植物組織透明化剤 |
| US11254974B2 (en) | 2016-02-10 | 2022-02-22 | The Board Of Trustees Of The Leland Stanford Junior University | RNA fixation and detection in clarity-based hydrogel tissue |
| WO2017188264A1 (ja) * | 2016-04-28 | 2017-11-02 | 国立研究開発法人理化学研究所 | 光透過性に優れた生物材料を調製するための組成物およびその利用 |
| CN109196093A (zh) * | 2016-08-10 | 2019-01-11 | 株式会社资生堂 | 透明皮肤试样 |
| JP2018044800A (ja) * | 2016-09-13 | 2018-03-22 | 国立大学法人 東京大学 | 生物試料の透明化方法 |
| CN106769298B (zh) * | 2016-12-12 | 2019-04-16 | 中国科学院植物研究所 | 一种植物组织的显微观察方法 |
| CN106901865B (zh) * | 2017-01-06 | 2019-10-01 | 华中科技大学 | 一种颅骨光透明处理方法及其应用 |
| US10995361B2 (en) | 2017-01-23 | 2021-05-04 | Massachusetts Institute Of Technology | Multiplexed signal amplified FISH via splinted ligation amplification and sequencing |
| CN107069391B (zh) * | 2017-02-10 | 2020-07-17 | 北京大学 | 飞秒脉冲激光调制器及具有其的微型双光子显微成像装置 |
| WO2018157074A1 (en) | 2017-02-24 | 2018-08-30 | Massachusetts Institute Of Technology | Methods for diagnosing neoplastic lesions |
| WO2018157048A1 (en) | 2017-02-24 | 2018-08-30 | Massachusetts Institute Of Technology | Methods for examining podocyte foot processes in human renal samples using conventional optical microscopy |
| CN107132101B (zh) * | 2017-03-28 | 2019-10-11 | 中国科学院深圳先进技术研究院 | 一种组织光透明剂及其制备方法和应用 |
| WO2019023214A1 (en) | 2017-07-25 | 2019-01-31 | Massachusetts Institute Of Technology | ATAC IN SITU SEQUENCING |
| US11873374B2 (en) | 2018-02-06 | 2024-01-16 | Massachusetts Institute Of Technology | Swellable and structurally homogenous hydrogels and methods of use thereof |
| WO2020013833A1 (en) | 2018-07-13 | 2020-01-16 | Massachusetts Institute Of Technology | Dimethylacrylamide (dmaa) hydrogel for expansion microscopy (exm) |
| CA3130889A1 (en) | 2019-02-22 | 2020-08-27 | Massachusetts Institute Of Technology | Iterative direct expansion microscopy |
| US12265004B2 (en) | 2019-11-05 | 2025-04-01 | Massachusetts Institute Of Technology | Membrane probes for expansion microscopy |
| WO2021113505A1 (en) | 2019-12-05 | 2021-06-10 | Massachusetts Institute Of Technology | Method for preparing a specimen for expansion microscopy |
| US12275920B2 (en) | 2020-06-28 | 2025-04-15 | Sunghee Lee Dimauro | Compositions and methods for clarification of biological materials |
| EP4016045B1 (de) * | 2020-12-17 | 2025-02-05 | MobiCron GmbH | Verfahren zur optischen klärung einer gewebeprobe mit einem einbettungsmedium |
| JPWO2022191336A1 (enExample) | 2021-03-12 | 2022-09-15 |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3883365A (en) * | 1972-01-04 | 1975-05-13 | Suomen Sokeri Oy | PH adjustment in fructose crystallization for increased yield |
| JPH0774121B2 (ja) | 1992-09-01 | 1995-08-09 | 哲 濱田 | 生物体の内部透視が可能な標本製造方法 |
| US6232092B1 (en) * | 1998-10-02 | 2001-05-15 | Rogers Imaging Corporation | Method for preparing biological specimens for visual analysis |
| US6472216B1 (en) | 2001-07-24 | 2002-10-29 | Ann-Shyn Chiang | Aqueous tissue clearing solution |
| US6703242B1 (en) * | 2002-01-02 | 2004-03-09 | Vladimir V. Senatorov | Dark-field microscopy visualization of unstained axonal pathways using oil of wintergreen |
| JP2009524826A (ja) | 2006-01-26 | 2009-07-02 | ザ ユニヴァーシティー オブ テキサス | 撮像のための方法及び装置 |
| JP5388090B2 (ja) * | 2008-05-29 | 2014-01-15 | 国立大学法人東北大学 | 強膜透明化による角膜移植材料調製方法 |
| CN108061677B (zh) * | 2010-03-12 | 2021-10-29 | 国立研究开发法人理化学研究所 | 用于生物材料的澄清试剂和其用途 |
| JP2012141488A (ja) | 2011-01-05 | 2012-07-26 | Smk Corp | 反強誘電性液晶素子 |
-
2012
- 2012-06-22 JP JP2012141488A patent/JP5967528B2/ja active Active
-
2013
- 2013-06-21 US US14/408,647 patent/US9933342B2/en active Active
- 2013-06-21 WO PCT/JP2013/067085 patent/WO2013191274A1/ja not_active Ceased
- 2013-06-21 EP EP13806589.1A patent/EP2866017B1/en active Active
-
2018
- 2018-02-21 US US15/901,618 patent/US10883903B2/en active Active
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