JP2014005231A5 - - Google Patents

Download PDF

Info

Publication number
JP2014005231A5
JP2014005231A5 JP2012141488A JP2012141488A JP2014005231A5 JP 2014005231 A5 JP2014005231 A5 JP 2014005231A5 JP 2012141488 A JP2012141488 A JP 2012141488A JP 2012141488 A JP2012141488 A JP 2012141488A JP 2014005231 A5 JP2014005231 A5 JP 2014005231A5
Authority
JP
Japan
Prior art keywords
biological material
solution
fructose
clarification
clearing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP2012141488A
Other languages
Japanese (ja)
Other versions
JP2014005231A (en
JP5967528B2 (en
Filing date
Publication date
Priority claimed from JP2012141488A external-priority patent/JP5967528B2/en
Priority to JP2012141488A priority Critical patent/JP5967528B2/en
Application filed filed Critical
Priority to US14/408,647 priority patent/US9933342B2/en
Priority to PCT/JP2013/067085 priority patent/WO2013191274A1/en
Priority to EP13806589.1A priority patent/EP2866017B1/en
Publication of JP2014005231A publication Critical patent/JP2014005231A/en
Publication of JP2014005231A5 publication Critical patent/JP2014005231A5/ja
Publication of JP5967528B2 publication Critical patent/JP5967528B2/en
Application granted granted Critical
Priority to US15/901,618 priority patent/US10883903B2/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Claims (15)

生物材料を透明化する方法であって、
フルクトースを含む溶液を生物材料中に浸潤させて、当該生物材料を透明化する工程、を含むことを特徴とする生物材料の透明化方法。 A method for making a biological material transparent,
A method for clarifying a biological material, comprising the step of infiltrating a biological material with a solution containing fructose to clarify the biological material. 上記溶液は、上記フルクトースを５０％（ｗ／ｖ）以上で２００％（ｗ／ｖ）以下の範囲内の濃度で含むことを特徴とする請求項１に記載の生物材料の透明化方法。   The method for clarification of a biological material according to claim 1, wherein the solution contains the fructose at a concentration in the range of 50% (w / v) to 200% (w / v). 上記溶液は、上記フルクトースを飽和濃度で含むことを特徴とする請求項１または２に記載の生物材料の透明化方法。   The method for clarifying biological material according to claim 1 or 2, wherein the solution contains the fructose at a saturated concentration. 上記溶液は、水溶液であることを特徴とする請求項１〜３の何れか１項に記載の生物材料の透明化方法。   The method for clarification of a biological material according to any one of claims 1 to 3, wherein the solution is an aqueous solution. 上記工程の前に、上記溶液より低い濃度でフルクトースを含む１以上の溶液を、濃度の低い順に、上記生物材料中に浸潤させる工程をさらに含むことを特徴とする請求項１〜４の何れか１項に記載の生物材料の透明化方法。   5. The method according to claim 1, further comprising a step of infiltrating the biological material with one or more solutions containing fructose at a concentration lower than that of the solution before the step. 2. A method for clarifying a biological material according to item 1. 上記溶液の何れもが、水溶液であることを特徴とする請求項５に記載の生物材料の透明化方法。   6. The method for clarifying a biological material according to claim 5, wherein any of the solutions is an aqueous solution. 上記生物材料は、多細胞動物由来の組織もしくは器官、またはヒトを除く多細胞動物の個体であることを特徴とする請求項１〜６の何れか１項に記載の生物材料の透明化方法。   The method for clarification of a biological material according to any one of claims 1 to 6, wherein the biological material is a tissue or organ derived from a multicellular animal, or an individual of a multicellular animal other than a human. 上記生物材料は、蛍光タンパク質を発現している細胞を含んでいることを特徴とする請求項７に記載の生物材料の透明化方法。   8. The biological material clarification method according to claim 7, wherein the biological material includes cells expressing a fluorescent protein. 生物材料用の透明化試薬であって、
フルクトースを含む溶液であることを特徴とする透明化試薬。 A clearing reagent for biological materials,
A clearing reagent, which is a solution containing fructose. 上記溶液は、上記フルクトースを５０％（ｗ／ｖ）以上で２００％（ｗ／ｖ）以下の範囲内の濃度で含むことを特徴とする請求項９に記載の透明化試薬。The clearing reagent according to claim 9, wherein the solution contains the fructose at a concentration within a range of 50% (w / v) to 200% (w / v). 上記溶液は、上記フルクトースを飽和濃度で含むことを特徴とする請求項９または１０に記載の透明化試薬。The clarification reagent according to claim 9 or 10, wherein the solution contains the fructose at a saturated concentration. 上記溶液は、水溶液であることを特徴とする請求項９〜１１の何れか１項に記載の透明化試薬。The clearing reagent according to any one of claims 9 to 11, wherein the solution is an aqueous solution. 生物材料用透明化処理キットであって、
フルクトースを含む溶液を備えることを特徴とする生物材料用透明化処理キット。 A biological material clearing treatment kit,
A biological material clarification treatment kit comprising a solution containing fructose. 上記溶液より低い濃度でフルクトースを含む１以上の溶液をさらに備えることを特徴とする請求項１３に記載の生物材料用透明化処理キット。The biological material clearing treatment kit according to claim 13, further comprising one or more solutions containing fructose at a lower concentration than the solution. 上記溶液の何れもが、水溶液であることを特徴とする請求項１４に記載の生物材料用透明化処理キット。All of the said solution is aqueous solution, The clearing treatment kit for biological materials of Claim 14 characterized by the above-mentioned.
JP2012141488A 2012-06-22 2012-06-22 Biological material clarification method and biological material clarification treatment kit Active JP5967528B2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
JP2012141488A JP5967528B2 (en) 2012-06-22 2012-06-22 Biological material clarification method and biological material clarification treatment kit
US14/408,647 US9933342B2 (en) 2012-06-22 2013-06-21 Method for rendering biological material transparent and processing kit for rendering biological material transparent
PCT/JP2013/067085 WO2013191274A1 (en) 2012-06-22 2013-06-21 Method for rendering biological material transparent and processing kit for rendering biological material transparent
EP13806589.1A EP2866017B1 (en) 2012-06-22 2013-06-21 Method for rendering biological material transparent
US15/901,618 US10883903B2 (en) 2012-06-22 2018-02-21 Method for rendering biological material transparent and processing kit for rendering biological material transparent

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2012141488A JP5967528B2 (en) 2012-06-22 2012-06-22 Biological material clarification method and biological material clarification treatment kit

Publications (3)

Publication Number Publication Date
JP2014005231A JP2014005231A (en) 2014-01-16
JP2014005231A5 true JP2014005231A5 (en) 2015-06-25
JP5967528B2 JP5967528B2 (en) 2016-08-10

Family

ID=49768865

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2012141488A Active JP5967528B2 (en) 2012-06-22 2012-06-22 Biological material clarification method and biological material clarification treatment kit

Country Status (4)

Country Link
US (2) US9933342B2 (en)
EP (1) EP2866017B1 (en)
JP (1) JP5967528B2 (en)
WO (1) WO2013191274A1 (en)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2711682B1 (en) 2011-05-20 2019-07-10 Riken Clarifying reagent for biological materials and use thereof
AU2013300171B2 (en) 2012-08-09 2017-09-14 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for preparing biological specimens for microscopic analysis
WO2015127183A2 (en) 2014-02-21 2015-08-27 Massachusetts Institute Of Technology Expansion microscopy
AU2015266894A1 (en) 2014-05-30 2016-08-25 The Board Of Trustees Of The Leland Stanford Junior University Methods and devices for imaging large intact tissue samples
EP3167272B8 (en) * 2014-07-07 2022-03-23 Chih-Yung Lin Aqueous tissue clearing solution and uses thereof
JP6687949B2 (en) * 2015-01-20 2020-04-28 国立研究開発法人理化学研究所 Clearing reagent for biological materials, system and use thereof
EP3273218A4 (en) * 2015-03-18 2018-12-26 Riken Method for observing biological material and clearing method
US10526649B2 (en) 2015-04-14 2020-01-07 Massachusetts Institute Of Technology Augmenting in situ nucleic acid sequencing of expanded biological samples with in vitro sequence information
US11408890B2 (en) 2015-04-14 2022-08-09 Massachusetts Institute Of Technology Iterative expansion microscopy
US10059990B2 (en) 2015-04-14 2018-08-28 Massachusetts Institute Of Technology In situ nucleic acid sequencing of expanded biological samples
CA2994957A1 (en) 2015-08-07 2017-02-16 Massachusetts Institute Of Technology Protein retention expansion microscopy
CN108474029B (en) 2015-08-07 2021-07-23 麻省理工学院 Nanoscale imaging of proteins and nucleic acids by extended microscopy
US20180348104A1 (en) * 2015-12-01 2018-12-06 Universitaet Duisburg-Essen Non-hazardous optical clearing of biological samples
JP6601842B2 (en) * 2015-12-17 2019-11-06 国立大学法人名古屋大学 Plant tissue clarifier
US11254974B2 (en) 2016-02-10 2022-02-22 The Board Of Trustees Of The Leland Stanford Junior University RNA fixation and detection in clarity-based hydrogel tissue
EP3450953A4 (en) * 2016-04-28 2020-01-08 Riken Composition for preparing biological material having excellent light transmissivity and use of composition
EP3498823A4 (en) 2016-08-10 2020-02-05 Shiseido Company Ltd. Transparent skin sample
JP2018044800A (en) * 2016-09-13 2018-03-22 国立大学法人 東京大学 Transparentizing method of biological sample
CN106769298B (en) * 2016-12-12 2019-04-16 中国科学院植物研究所 A kind of microscopy observation method of plant tissue
CN106901865B (en) * 2017-01-06 2019-10-01 华中科技大学 A kind of skull light transparent processing method and its application
WO2018136856A1 (en) 2017-01-23 2018-07-26 Massachusetts Institute Of Technology Multiplexed signal amplified fish via splinted ligation amplification and sequencing
CN107069391B (en) * 2017-02-10 2020-07-17 北京大学 Femtosecond pulse laser modulator and miniature two-photon microscopic imaging device with same
WO2018157048A1 (en) 2017-02-24 2018-08-30 Massachusetts Institute Of Technology Methods for examining podocyte foot processes in human renal samples using conventional optical microscopy
CN107132101B (en) * 2017-03-28 2019-10-11 中国科学院深圳先进技术研究院 A kind of tissue light clarifier and its preparation method and application
US11180804B2 (en) 2017-07-25 2021-11-23 Massachusetts Institute Of Technology In situ ATAC sequencing
WO2019156957A1 (en) 2018-02-06 2019-08-15 Massachusetts Institute Of Technology Swellable and structurally homogenous hydrogels and methods of use thereof
US11802822B2 (en) 2019-12-05 2023-10-31 Massachusetts Institute Of Technology Multiplexed expansion (MultiExM) pathology
US20240167922A1 (en) 2021-03-12 2024-05-23 Kyoto University Transparentizing reagent and transparentizing method

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3883365A (en) 1972-01-04 1975-05-13 Suomen Sokeri Oy PH adjustment in fructose crystallization for increased yield
JPH0774121B2 (en) 1992-09-01 1995-08-09 哲 濱田 Specimen manufacturing method capable of seeing through an organism
US6232092B1 (en) * 1998-10-02 2001-05-15 Rogers Imaging Corporation Method for preparing biological specimens for visual analysis
US6472216B1 (en) 2001-07-24 2002-10-29 Ann-Shyn Chiang Aqueous tissue clearing solution
US6703242B1 (en) 2002-01-02 2004-03-09 Vladimir V. Senatorov Dark-field microscopy visualization of unstained axonal pathways using oil of wintergreen
US8189737B2 (en) * 2006-01-26 2012-05-29 The Board Of Regents, The University Of Texas System, A Texas Institution Of Higher Learning Process and apparatus for microCT imaging of ex vivo specimens
JP5388090B2 (en) 2008-05-29 2014-01-15 国立大学法人東北大学 Preparation method of corneal transplant material by scleral transparency
US20130045503A1 (en) * 2010-03-12 2013-02-21 Riken Clearing reagent for biological material, and use thereof
JP2012141488A (en) 2011-01-05 2012-07-26 Smk Corp Antiferroelectric liquid crystal element

Similar Documents

Publication Publication Date Title
JP2014005231A5 (en)
JP2020031649A5 (en)
WO2016015058A3 (en) Methods of determining tissues and/or cell types giving rise to cell-free dna, and methods of identifying a disease or disorder using same
EP4248740A3 (en) Humanized sirpa-il15 knockin mice and methods of use thereof
AR088514A1 (en) BISPECIFIC IMMUNOLIGANTS DIRECTED AGAINST TNF
WO2011116238A3 (en) Light-sensitive ion-passing molecules
EA201201659A1 (en) WATER COMPOSITION CONTAINING BROMHEXIN
ATE442590T1 (en) METHOD FOR THE RAPID DIAGNOSIS OF TARGETS IN HUMAN BODY FLUID
EA201591131A1 (en) DNA STRUCTURE ANTIBODY AND METHOD OF THEIR APPLICATION
CL2011000090A1 (en) Compounds derived from substituted fatty acids interrupted by a sulfur group; food pharmaceutical composition; method of preparation of these; and its use in the treatment of metabolic, cardiovascular diseases, among others.
UA116618C2 (en) CONSTITUTION-BASED CYSTEINE ANTIBODY AGAINST CD79b
EA201270304A1 (en) WAYS OF OBTAINING SILK PASTE
JP2009178558A5 (en)
JP2016520068A5 (en)
JP2014095726A5 (en)
EA201690503A1 (en) ANTIBODIES
BR112018012070A2 (en) method for the production of tissues / organs using blood cells
MX366369B (en) Method for a cell-based drug screening assay and the use thereof.
EA201390713A1 (en) ANTI-INFLAMMATORY PROTEINS AND METHODS FOR THEIR RECEPTION AND APPLICATION
MX2015015274A (en) Novel organic acid pathway.
FR2963357B1 (en) PHOTOLUMINESCENT MATERIAL CONTAINING SILVER ION
EA201590088A1 (en) Stem cells and pancreatic cells used for the treatment of insulin-dependent diabetes mellitus
EA201401117A1 (en) COMPOSITIONS CONTAINING PROBIOTICS AND COMPLEX OF BEE POULTRY / CLAY, METHOD OF THEIR PREPARATION AND THEIR APPLICATION IN FOOD AND THERAPY
EA201691563A1 (en) DRY ACID CONCENTRATE IN GRANULATED FORM
Al Rwahnih et al. Association of a circular DNA virus in grapevines affected by red blotch disease in California