JP5967528B2 - 生物材料を透明化する方法および生物材料用透明化処理キット - Google Patents

生物材料を透明化する方法および生物材料用透明化処理キット Download PDF

Info

Publication number
JP5967528B2
JP5967528B2 JP2012141488A JP2012141488A JP5967528B2 JP 5967528 B2 JP5967528 B2 JP 5967528B2 JP 2012141488 A JP2012141488 A JP 2012141488A JP 2012141488 A JP2012141488 A JP 2012141488A JP 5967528 B2 JP5967528 B2 JP 5967528B2
Authority
JP
Japan
Prior art keywords
biological material
fructose
clearing
concentration
solution
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2012141488A
Other languages
English (en)
Japanese (ja)
Other versions
JP2014005231A (ja
JP2014005231A5 (enExample
Inventor
猛 今井
猛 今井
孟岑 柯
孟岑 柯
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
RIKEN
Original Assignee
RIKEN
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by RIKEN filed Critical RIKEN
Priority to JP2012141488A priority Critical patent/JP5967528B2/ja
Priority to EP13806589.1A priority patent/EP2866017B1/en
Priority to PCT/JP2013/067085 priority patent/WO2013191274A1/ja
Priority to US14/408,647 priority patent/US9933342B2/en
Publication of JP2014005231A publication Critical patent/JP2014005231A/ja
Publication of JP2014005231A5 publication Critical patent/JP2014005231A5/ja
Application granted granted Critical
Publication of JP5967528B2 publication Critical patent/JP5967528B2/ja
Priority to US15/901,618 priority patent/US10883903B2/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/34—Microscope slides, e.g. mounting specimens on microscope slides
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/36—Embedding or analogous mounting of samples

Landscapes

  • Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Optics & Photonics (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
JP2012141488A 2012-06-22 2012-06-22 生物材料を透明化する方法および生物材料用透明化処理キット Active JP5967528B2 (ja)

Priority Applications (5)

Application Number Priority Date Filing Date Title
JP2012141488A JP5967528B2 (ja) 2012-06-22 2012-06-22 生物材料を透明化する方法および生物材料用透明化処理キット
EP13806589.1A EP2866017B1 (en) 2012-06-22 2013-06-21 Method for rendering biological material transparent
PCT/JP2013/067085 WO2013191274A1 (ja) 2012-06-22 2013-06-21 生物材料を透明化する方法および生物材料用透明化処理キット
US14/408,647 US9933342B2 (en) 2012-06-22 2013-06-21 Method for rendering biological material transparent and processing kit for rendering biological material transparent
US15/901,618 US10883903B2 (en) 2012-06-22 2018-02-21 Method for rendering biological material transparent and processing kit for rendering biological material transparent

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2012141488A JP5967528B2 (ja) 2012-06-22 2012-06-22 生物材料を透明化する方法および生物材料用透明化処理キット

Publications (3)

Publication Number Publication Date
JP2014005231A JP2014005231A (ja) 2014-01-16
JP2014005231A5 JP2014005231A5 (enExample) 2015-06-25
JP5967528B2 true JP5967528B2 (ja) 2016-08-10

Family

ID=49768865

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2012141488A Active JP5967528B2 (ja) 2012-06-22 2012-06-22 生物材料を透明化する方法および生物材料用透明化処理キット

Country Status (4)

Country Link
US (2) US9933342B2 (enExample)
EP (1) EP2866017B1 (enExample)
JP (1) JP5967528B2 (enExample)
WO (1) WO2013191274A1 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2022191336A1 (ja) 2021-03-12 2022-09-15 国立大学法人京都大学 透明化試薬および透明化方法

Families Citing this family (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2711682B1 (en) 2011-05-20 2019-07-10 Riken Clarifying reagent for biological materials and use thereof
EP4163617A1 (en) 2012-08-09 2023-04-12 The Board of Trustees of the Leland Stanford Junior University Methods and compositions for preparing biological specimens for microscopic analysis
JP6456969B2 (ja) * 2014-02-21 2019-01-23 マサチューセッツ インスティテュート オブ テクノロジー 膨張顕微鏡法
CA2947898A1 (en) 2014-05-30 2015-12-03 The Board Of Trustees Of The Leland Stanford Junior University Methods and devices for imaging large intact tissue samples
WO2016004563A1 (en) * 2014-07-07 2016-01-14 Chih-Yung Lin Aqueous tissue clearing solution and uses thereof
WO2016117614A1 (ja) * 2015-01-20 2016-07-28 国立研究開発法人理化学研究所 生物材料用透明化試薬、システム及びその利用
JP6781421B2 (ja) * 2015-03-18 2020-11-04 国立研究開発法人理化学研究所 生物材料の観察方法および透明化方法
US11408890B2 (en) 2015-04-14 2022-08-09 Massachusetts Institute Of Technology Iterative expansion microscopy
US10526649B2 (en) 2015-04-14 2020-01-07 Massachusetts Institute Of Technology Augmenting in situ nucleic acid sequencing of expanded biological samples with in vitro sequence information
US10059990B2 (en) 2015-04-14 2018-08-28 Massachusetts Institute Of Technology In situ nucleic acid sequencing of expanded biological samples
WO2017027368A1 (en) 2015-08-07 2017-02-16 Massachusetts Institute Of Technology Protein retention expansion microscopy
CN108474029B (zh) 2015-08-07 2021-07-23 麻省理工学院 通过扩展显微法的蛋白质和核酸的纳米级成像
WO2017093323A1 (en) * 2015-12-01 2017-06-08 Universität Duisburg-Essen Non-hazardous optical clearing of biological samples
JP6601842B2 (ja) * 2015-12-17 2019-11-06 国立大学法人名古屋大学 植物組織透明化剤
US11254974B2 (en) 2016-02-10 2022-02-22 The Board Of Trustees Of The Leland Stanford Junior University RNA fixation and detection in clarity-based hydrogel tissue
US11656159B2 (en) * 2016-04-28 2023-05-23 Riken Composition for preparing biological material having excellent light transmissivity and use of composition
CN109196093A (zh) * 2016-08-10 2019-01-11 株式会社资生堂 透明皮肤试样
JP2018044800A (ja) * 2016-09-13 2018-03-22 国立大学法人 東京大学 生物試料の透明化方法
CN106769298B (zh) * 2016-12-12 2019-04-16 中国科学院植物研究所 一种植物组织的显微观察方法
CN106901865B (zh) * 2017-01-06 2019-10-01 华中科技大学 一种颅骨光透明处理方法及其应用
WO2018136856A1 (en) 2017-01-23 2018-07-26 Massachusetts Institute Of Technology Multiplexed signal amplified fish via splinted ligation amplification and sequencing
CN107069391B (zh) * 2017-02-10 2020-07-17 北京大学 飞秒脉冲激光调制器及具有其的微型双光子显微成像装置
WO2018157048A1 (en) 2017-02-24 2018-08-30 Massachusetts Institute Of Technology Methods for examining podocyte foot processes in human renal samples using conventional optical microscopy
WO2018157074A1 (en) 2017-02-24 2018-08-30 Massachusetts Institute Of Technology Methods for diagnosing neoplastic lesions
CN107132101B (zh) * 2017-03-28 2019-10-11 中国科学院深圳先进技术研究院 一种组织光透明剂及其制备方法和应用
US11180804B2 (en) 2017-07-25 2021-11-23 Massachusetts Institute Of Technology In situ ATAC sequencing
US11873374B2 (en) 2018-02-06 2024-01-16 Massachusetts Institute Of Technology Swellable and structurally homogenous hydrogels and methods of use thereof
WO2020013833A1 (en) 2018-07-13 2020-01-16 Massachusetts Institute Of Technology Dimethylacrylamide (dmaa) hydrogel for expansion microscopy (exm)
CA3130889A1 (en) 2019-02-22 2020-08-27 Massachusetts Institute Of Technology Iterative direct expansion microscopy
US12265004B2 (en) 2019-11-05 2025-04-01 Massachusetts Institute Of Technology Membrane probes for expansion microscopy
US11802822B2 (en) 2019-12-05 2023-10-31 Massachusetts Institute Of Technology Multiplexed expansion (MultiExM) pathology
US12275920B2 (en) 2020-06-28 2025-04-15 Sunghee Lee Dimauro Compositions and methods for clarification of biological materials
EP4016045B1 (de) * 2020-12-17 2025-02-05 MobiCron GmbH Verfahren zur optischen klärung einer gewebeprobe mit einem einbettungsmedium

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3883365A (en) * 1972-01-04 1975-05-13 Suomen Sokeri Oy PH adjustment in fructose crystallization for increased yield
JPH0774121B2 (ja) * 1992-09-01 1995-08-09 哲 濱田 生物体の内部透視が可能な標本製造方法
US6232092B1 (en) * 1998-10-02 2001-05-15 Rogers Imaging Corporation Method for preparing biological specimens for visual analysis
US6472216B1 (en) 2001-07-24 2002-10-29 Ann-Shyn Chiang Aqueous tissue clearing solution
US6703242B1 (en) * 2002-01-02 2004-03-09 Vladimir V. Senatorov Dark-field microscopy visualization of unstained axonal pathways using oil of wintergreen
AU2007210068A1 (en) * 2006-01-26 2007-08-09 The Board Of Regents, The University Of Texas System Process and apparatus for imaging
JP5388090B2 (ja) * 2008-05-29 2014-01-15 国立大学法人東北大学 強膜透明化による角膜移植材料調製方法
WO2011111876A1 (en) * 2010-03-12 2011-09-15 Riken Clearing reagent for biological material, and use thereof
JP2012141488A (ja) 2011-01-05 2012-07-26 Smk Corp 反強誘電性液晶素子

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2022191336A1 (ja) 2021-03-12 2022-09-15 国立大学法人京都大学 透明化試薬および透明化方法

Also Published As

Publication number Publication date
US20180180520A1 (en) 2018-06-28
US20160169776A1 (en) 2016-06-16
WO2013191274A1 (ja) 2013-12-27
EP2866017B1 (en) 2021-09-22
JP2014005231A (ja) 2014-01-16
EP2866017A1 (en) 2015-04-29
EP2866017A4 (en) 2016-02-24
US9933342B2 (en) 2018-04-03
US10883903B2 (en) 2021-01-05

Similar Documents

Publication Publication Date Title
JP5967528B2 (ja) 生物材料を透明化する方法および生物材料用透明化処理キット
JP5858435B2 (ja) 生物材料用透明化試薬、及びその利用
JP5924624B2 (ja) 生物材料を透明化する方法、及びその利用
CN107300496B (zh) 生物材料用透明化试剂、及其利用
JP6781421B2 (ja) 生物材料の観察方法および透明化方法
JP6687949B2 (ja) 生物材料用透明化試薬、システム及びその利用
Jalufka et al. Hydrophobic and hydrogel-based methods for passive tissue clearing
Burnett et al. Hydrogel encapsulation of living organisms for long-term microscopy
Gensbigler et al. An optimized, high‐throughput workflow for the collection, processing, and visualization of histology data in comparative vertebrate morphogenesis
KR20250081553A (ko) 생체조직 투명화 가속화 용액을 포함하는 생체조직 투명화용 키트, 생체조직 투명화용 전기영동 장치 및 이를 이용한 생체조직 투명화 방법
KR20240000007A (ko) 생체조직 투명화 조성물 및 이를 이용한 전기영동 투명화 방법
Jillson Ependymin Immunoreactivity in Limulus polyphemus

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20150507

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20150507

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20160301

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20160607

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20160624

R150 Certificate of patent or registration of utility model

Ref document number: 5967528

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250