JP2013531983A - 多重生物検出のための核酸ならびにその使用および製造方法 - Google Patents

多重生物検出のための核酸ならびにその使用および製造方法 Download PDF

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JP2013531983A
JP2013531983A JP2013514408A JP2013514408A JP2013531983A JP 2013531983 A JP2013531983 A JP 2013531983A JP 2013514408 A JP2013514408 A JP 2013514408A JP 2013514408 A JP2013514408 A JP 2013514408A JP 2013531983 A JP2013531983 A JP 2013531983A
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ダイアモンド,リサ
クム,ヨッヒェン
ロルフ,フィリップ,アレクサンダー
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Pathogenica inc
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701Specific hybridization probes
    • C12Q1/708Specific hybridization probes for papilloma
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    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • C12Q1/701Specific hybridization probes

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JP2013514408A 2010-06-11 2011-06-10 多重生物検出のための核酸ならびにその使用および製造方法 Pending JP2013531983A (ja)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US35401110P 2010-06-11 2010-06-11
US61/354,011 2010-06-11
US37404110P 2010-08-16 2010-08-16
US61/374,041 2010-08-16
US201161439167P 2011-02-03 2011-02-03
US61/439,167 2011-02-03
PCT/US2011/040106 WO2011156795A2 (en) 2010-06-11 2011-06-10 Nucleic acids for multiplex organism detection and methods of use and making the same

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JP2013531983A true JP2013531983A (ja) 2013-08-15
JP2013531983A5 JP2013531983A5 (https=) 2014-09-04

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US (1) US20130261196A1 (https=)
EP (1) EP2580354A4 (https=)
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AU (1) AU2011265205A1 (https=)
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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2016528913A (ja) * 2013-08-26 2016-09-23 ザ・トランスレーショナル・ジェノミクス・リサーチ・インスティチュート 病原体試料におけるマイナー変異体突然変異検出のための単一分子重複読み取り分析
TWI577803B (zh) * 2015-01-15 2017-04-11 昕穎生醫技術股份有限公司 多重抗藥性結核病篩檢方法及套組
JP2017525339A (ja) * 2014-06-24 2017-09-07 アンスティテュ パストゥール 1又は複数の生物由来の染色体の断片をアセンブルする方法、装置、及びコンピュータプログラム

Families Citing this family (56)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9562837B2 (en) 2006-05-11 2017-02-07 Raindance Technologies, Inc. Systems for handling microfludic droplets
EP2315629B1 (en) 2008-07-18 2021-12-15 Bio-Rad Laboratories, Inc. Droplet libraries
US12038438B2 (en) 2008-07-18 2024-07-16 Bio-Rad Laboratories, Inc. Enzyme quantification
US9399797B2 (en) 2010-02-12 2016-07-26 Raindance Technologies, Inc. Digital analyte analysis
US10351905B2 (en) * 2010-02-12 2019-07-16 Bio-Rad Laboratories, Inc. Digital analyte analysis
CA2789425C (en) 2010-02-12 2020-04-28 Raindance Technologies, Inc. Digital analyte analysis with polymerase error correction
EP3736281A1 (en) 2011-02-18 2020-11-11 Bio-Rad Laboratories, Inc. Compositions and methods for molecular labeling
WO2013173774A2 (en) * 2012-05-18 2013-11-21 Pathogenica, Inc. Molecular inversion probes
WO2013173795A1 (en) * 2012-05-18 2013-11-21 Pathogenica, Inc. Realtime sequence based biosurveillance system
CN105190656B (zh) 2013-01-17 2018-01-16 佩索纳里斯公司 用于遗传分析的方法和系统
EP3988671A1 (en) * 2013-02-20 2022-04-27 Emory University Compositions for sequencing nucleic acids in mixtures
AU2013382195B2 (en) 2013-03-13 2019-09-19 Illumina, Inc. Methods and systems for aligning repetitive DNA elements
CA2905410A1 (en) * 2013-03-15 2014-09-25 Abbott Molecular Inc. Systems and methods for detection of genomic copy number changes
US20150141257A1 (en) * 2013-08-02 2015-05-21 Roche Nimblegen, Inc. Sequence capture method using specialized capture probes (heatseq)
WO2015031689A1 (en) 2013-08-30 2015-03-05 Personalis, Inc. Methods and systems for genomic analysis
WO2015071552A1 (en) * 2013-11-18 2015-05-21 Teknologian Tutkimuskeskus Vtt Multi-unit probes with high specificity and a method of designing the same
EP3129503A4 (en) 2014-04-11 2018-02-21 The Trustees Of The University Of Pennsylvania Compositions and methods for metagenome biomarker detection
KR102535489B1 (ko) 2014-06-13 2023-05-22 큐-리네아 에이비 미생물 검출 방법 및 특성 규명 방법
US10125399B2 (en) 2014-10-30 2018-11-13 Personalis, Inc. Methods for using mosaicism in nucleic acids sampled distal to their origin
GB201507026D0 (en) 2015-04-24 2015-06-10 Linea Ab Q Medical sample transportation container
EP3362580B1 (en) * 2015-10-18 2021-02-17 Affymetrix, Inc. Multiallelic genotyping of single nucleotide polymorphisms and indels
CA3185611A1 (en) 2016-03-25 2017-09-28 Karius, Inc. Synthetic nucleic acid spike-ins
GB2554767A (en) 2016-04-21 2018-04-11 Q Linea Ab Detecting and characterising a microorganism
US11299783B2 (en) 2016-05-27 2022-04-12 Personalis, Inc. Methods and systems for genetic analysis
US10337051B2 (en) * 2016-06-16 2019-07-02 The Regents Of The University Of California Methods and compositions for detecting a target RNA
CA3038960A1 (en) 2016-09-30 2018-04-05 The Regents Of The University Of California Rna-guided nucleic acid modifying enzymes and methods of use thereof
JP7306696B2 (ja) 2016-09-30 2023-07-11 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア Rna誘導型核酸修飾酵素及びその使用方法
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
JP7240733B2 (ja) * 2017-05-23 2023-03-16 ラトガース,ザ ステート ユニバーシティ オブ ニュー ジャージー デュアル相互作用ヘアピンプローブを使用した標的媒介in situシグナル増幅
US11430543B2 (en) 2017-08-04 2022-08-30 Billiontoone, Inc. Sequencing output determination and analysis with target-associated molecules in quantification associated with biological targets
US11519024B2 (en) 2017-08-04 2022-12-06 Billiontoone, Inc. Homologous genomic regions for characterization associated with biological targets
EP3662480A4 (en) 2017-08-04 2021-05-19 BillionToOne, Inc. MOLECULES ASSOCIATED WITH A TARGET FOR A CHARACTERIZATION ASSOCIATED WITH BIOLOGICAL TARGETS
WO2019089808A1 (en) 2017-11-01 2019-05-09 The Regents Of The University Of California Class 2 crispr/cas compositions and methods of use
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WO2019089804A1 (en) 2017-11-01 2019-05-09 The Regents Of The University Of California Casy compositions and methods of use
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WO2019157529A1 (en) 2018-02-12 2019-08-15 10X Genomics, Inc. Methods characterizing multiple analytes from individual cells or cell populations
US11639928B2 (en) 2018-02-22 2023-05-02 10X Genomics, Inc. Methods and systems for characterizing analytes from individual cells or cell populations
WO2019169028A1 (en) 2018-02-28 2019-09-06 10X Genomics, Inc. Transcriptome sequencing through random ligation
WO2019226648A1 (en) * 2018-05-21 2019-11-28 Battelle Memorial Institute Methods and control compositions for sequencing and chemical analyses
US10801064B2 (en) * 2018-05-31 2020-10-13 Personalis, Inc. Compositions, methods and systems for processing or analyzing multi-species nucleic acid samples
EP3830301B1 (en) 2018-08-01 2024-05-22 Mammoth Biosciences, Inc. Programmable nuclease compositions and methods of use thereof
US12071651B2 (en) 2018-08-06 2024-08-27 Billiontoone, Inc. Dilution tagging for quantification of biological targets
ES3059710T3 (en) 2018-11-21 2026-03-23 Karius Inc Direct-to-library methods, systems, and compositions
WO2020124003A1 (en) 2018-12-13 2020-06-18 Battelle Memorial Institute Methods and control compositions for a quantitative polymerase chain reaction
EP3931313A2 (en) 2019-01-04 2022-01-05 Mammoth Biosciences, Inc. Programmable nuclease improvements and compositions and methods for nucleic acid amplification and detection
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EP4055610A4 (en) 2019-11-05 2023-11-29 Personalis, Inc. ESTIMATION OF TUMOR PURITY FROM SINGLE SAMPLES
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EP4108783B1 (en) * 2021-06-24 2024-02-21 Miltenyi Biotec B.V. & Co. KG Spatial sequencing with mictag
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JP2025509878A (ja) 2022-03-21 2025-04-11 ビリオントゥーワン,インコーポレイテッド 処置モニタリングのためのメチル化セルフリーdnaの分子計数

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2004528016A (ja) * 2000-10-24 2004-09-16 ザ ボード オブ トラスティーズ オブ ザ リーランド スタンフォード ジュニア ユニバーシティ ゲノムdnaの直接多重処理による性状分析

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030134293A1 (en) * 1999-11-16 2003-07-17 Zhiping Liu Method for rapid and accurate identification of microorganisms
EP1522853A4 (en) * 2002-06-24 2005-10-19 Canon Kk DNA MICROARRAY WITH STANDARD PROBE AND KIT CONTAINING THE MICROARRAY
US7618780B2 (en) * 2004-05-20 2009-11-17 Trillion Genomics Limited Use of mass labelled probes to detect target nucleic acids using mass spectrometry
WO2007106407A2 (en) * 2006-03-10 2007-09-20 Wyeth Microarray for monitoring gene expression in multiple strains of streptococcus pneumoniae
US7897747B2 (en) * 2006-05-25 2011-03-01 The Board Of Trustees Of The Leland Stanford Junior University Method to produce single stranded DNA of defined length and sequence and DNA probes produced thereby
WO2010142017A1 (en) * 2009-06-09 2010-12-16 Defyrus, Inc . Administration of interferon for prophylaxis against or treatment of pathogenic infection

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2004528016A (ja) * 2000-10-24 2004-09-16 ザ ボード オブ トラスティーズ オブ ザ リーランド スタンフォード ジュニア ユニバーシティ ゲノムdnaの直接多重処理による性状分析

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
JPN6015031685; Nucl. Acids Res. vol.18,no.7, 1990, p1757-1761 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2016528913A (ja) * 2013-08-26 2016-09-23 ザ・トランスレーショナル・ジェノミクス・リサーチ・インスティチュート 病原体試料におけるマイナー変異体突然変異検出のための単一分子重複読み取り分析
JP2017525339A (ja) * 2014-06-24 2017-09-07 アンスティテュ パストゥール 1又は複数の生物由来の染色体の断片をアセンブルする方法、装置、及びコンピュータプログラム
TWI577803B (zh) * 2015-01-15 2017-04-11 昕穎生醫技術股份有限公司 多重抗藥性結核病篩檢方法及套組

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WO2011156795A3 (en) 2012-04-05
US20130261196A1 (en) 2013-10-03
AU2011265205A1 (en) 2013-01-31
EP2580354A2 (en) 2013-04-17
EP2580354A4 (en) 2013-10-30
WO2011156795A2 (en) 2011-12-15
SG186987A1 (en) 2013-02-28

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