JP2013507971A5 - - Google Patents
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- JP2013507971A5 JP2013507971A5 JP2012535419A JP2012535419A JP2013507971A5 JP 2013507971 A5 JP2013507971 A5 JP 2013507971A5 JP 2012535419 A JP2012535419 A JP 2012535419A JP 2012535419 A JP2012535419 A JP 2012535419A JP 2013507971 A5 JP2013507971 A5 JP 2013507971A5
- Authority
- JP
- Japan
- Prior art keywords
- primer
- segment
- pcr
- amplification
- annealing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 claims description 37
- 230000003321 amplification Effects 0.000 claims description 36
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 36
- 238000000137 annealing Methods 0.000 claims description 29
- 238000006243 chemical reaction Methods 0.000 claims description 17
- 239000002773 nucleotide Substances 0.000 claims description 16
- 125000003729 nucleotide group Chemical group 0.000 claims description 16
- 230000000295 complement effect Effects 0.000 claims description 12
- 239000002299 complementary DNA Substances 0.000 claims description 10
- 108020004999 messenger RNA Proteins 0.000 claims description 7
- 108091034117 Oligonucleotide Proteins 0.000 claims description 6
- 238000004458 analytical method Methods 0.000 claims description 6
- 238000004925 denaturation Methods 0.000 claims description 6
- 230000036425 denaturation Effects 0.000 claims description 6
- 108020004707 nucleic acids Proteins 0.000 claims description 6
- 150000007523 nucleic acids Chemical class 0.000 claims description 6
- 102000039446 nucleic acids Human genes 0.000 claims description 6
- 238000002844 melting Methods 0.000 claims description 4
- 230000008018 melting Effects 0.000 claims description 4
- 238000003753 real-time PCR Methods 0.000 claims description 4
- 230000002194 synthesizing effect Effects 0.000 claims description 4
- 238000010839 reverse transcription Methods 0.000 claims description 3
- 230000004544 DNA amplification Effects 0.000 claims description 2
- 230000004543 DNA replication Effects 0.000 claims description 2
- 108700005084 Multigene Family Proteins 0.000 claims description 2
- 108091028664 Ribonucleotide Proteins 0.000 claims description 2
- 239000005547 deoxyribonucleotide Substances 0.000 claims description 2
- 125000002637 deoxyribonucleotide group Chemical group 0.000 claims description 2
- 230000029087 digestion Effects 0.000 claims description 2
- 238000007850 in situ PCR Methods 0.000 claims description 2
- 238000007403 mPCR Methods 0.000 claims description 2
- 239000002679 microRNA Substances 0.000 claims description 2
- 238000002703 mutagenesis Methods 0.000 claims description 2
- 231100000350 mutagenesis Toxicity 0.000 claims description 2
- 108090000623 proteins and genes Proteins 0.000 claims description 2
- 238000012340 reverse transcriptase PCR Methods 0.000 claims description 2
- 239000002336 ribonucleotide Substances 0.000 claims description 2
- 125000002652 ribonucleotide group Chemical group 0.000 claims description 2
- 238000010532 solid phase synthesis reaction Methods 0.000 claims description 2
- 238000007862 touchdown PCR Methods 0.000 claims description 2
- 230000035897 transcription Effects 0.000 claims description 2
- 238000013518 transcription Methods 0.000 claims description 2
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 1
- 238000007039 two-step reaction Methods 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US25428109P | 2009-10-23 | 2009-10-23 | |
| US61/254,281 | 2009-10-23 | ||
| PCT/US2010/053773 WO2011050278A1 (en) | 2009-10-23 | 2010-10-22 | Amplification primers with non-standard bases for increased reaction specificity |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2013507971A JP2013507971A (ja) | 2013-03-07 |
| JP2013507971A5 true JP2013507971A5 (https=) | 2014-02-13 |
| JP5938348B2 JP5938348B2 (ja) | 2016-06-22 |
Family
ID=43898754
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2012535419A Expired - Fee Related JP5938348B2 (ja) | 2009-10-23 | 2010-10-22 | 反応特異性の増加のための非標準塩基を含む増幅プライマー |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US8440406B2 (https=) |
| EP (1) | EP2491146B1 (https=) |
| JP (1) | JP5938348B2 (https=) |
| CN (1) | CN102639712A (https=) |
| CA (1) | CA2778449C (https=) |
| WO (1) | WO2011050278A1 (https=) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2582602T3 (es) | 2007-03-28 | 2016-09-14 | Signal Diagnostics | Sistema y método de análisis de alta resolución de ácidos nucleicos para detectar variaciones de secuencia |
| US10669574B2 (en) | 2008-11-18 | 2020-06-02 | XCR Diagnostics, Inc. | DNA amplification technology |
| KR102351434B1 (ko) | 2013-02-07 | 2022-01-17 | 러트거즈,더스테이트유니버시티오브뉴저지 | 고도로 선택적인 핵산 증폭 프라이머 |
| EP2971144B1 (en) * | 2013-03-14 | 2019-10-30 | Aegea Biotechnologies, Inc. | Method for amplification of nucleic acids on solid support |
| CN105555971B (zh) | 2013-08-09 | 2017-08-29 | 卢米耐克斯公司 | 用于在核酸测定中改善熔链分辨和多重性的探针 |
| KR102323375B1 (ko) | 2013-10-09 | 2021-11-08 | 플루어레센트릭 인코포레이티드 | 다중 프로브 |
| CN106687589B (zh) * | 2014-07-10 | 2021-05-07 | 弗洛雷森特里克公司 | Dna扩增技术 |
| US10364458B2 (en) | 2014-07-16 | 2019-07-30 | Tangen Biosciences, Inc. | Isothermal methods for amplifying nucleic acid samples |
| EP3180449B1 (en) | 2014-08-11 | 2019-10-09 | Luminex Corporation | Probes for improved melt discrimination and multiplexing in nucleic acid assays |
| EP3215260B1 (en) | 2014-11-03 | 2020-01-15 | Tangen Biosciences Inc. | Apparatus and method for cell, spore, or virus capture and disruption |
| PL3298162T3 (pl) * | 2015-05-18 | 2020-07-27 | Saga Diagnostics Ab | Wykrywanie docelowego kwasu nukleinowego i wariantów |
| CN109234456B (zh) * | 2018-10-23 | 2023-06-09 | 深圳市亿立方生物技术有限公司 | 一种可同时检测6种呼吸道病原体的试剂盒及其应用 |
| WO2024170770A1 (en) | 2023-02-16 | 2024-08-22 | Asecud Sa | High nucleic acid sequence discrimination using a multifaceted nucleic acid molecule |
| WO2025023659A1 (ko) | 2023-07-25 | 2025-01-30 | 주식회사 씨젠 | 타겟 핵산 서열에 대한 올리고뉴클레오타이드 서열의 얼라인먼트 정보를 제공하는 컴퓨터-구현 방법 |
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| US5928869A (en) | 1997-05-30 | 1999-07-27 | Becton, Dickinson And Company | Detection of nucleic acids by fluorescence quenching |
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| US20070264694A1 (en) * | 2006-04-07 | 2007-11-15 | Eragen Biosciences, Inc. | Use of non-standard bases and proximity effects for gene assembly and conversion of non-standard bases to standard bases during dna synthesis |
| US20080076909A1 (en) * | 2006-06-30 | 2008-03-27 | Applera Corporation | Emulsion pcr and amplicon capture |
| CN102066574A (zh) * | 2008-06-17 | 2011-05-18 | 史蒂文·埃尔伯特·奔纳 | 利用非天然核苷酸扩增dna的过程 |
-
2010
- 2010-10-22 CA CA2778449A patent/CA2778449C/en active Active
- 2010-10-22 EP EP10825752.8A patent/EP2491146B1/en active Active
- 2010-10-22 US US12/910,113 patent/US8440406B2/en active Active
- 2010-10-22 WO PCT/US2010/053773 patent/WO2011050278A1/en not_active Ceased
- 2010-10-22 JP JP2012535419A patent/JP5938348B2/ja not_active Expired - Fee Related
- 2010-10-22 CN CN2010800475424A patent/CN102639712A/zh active Pending
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