CA2778449C - Amplification primers with non-standard bases for increased reaction specificity - Google Patents

Amplification primers with non-standard bases for increased reaction specificity Download PDF

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Publication number
CA2778449C
CA2778449C CA2778449A CA2778449A CA2778449C CA 2778449 C CA2778449 C CA 2778449C CA 2778449 A CA2778449 A CA 2778449A CA 2778449 A CA2778449 A CA 2778449A CA 2778449 C CA2778449 C CA 2778449C
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Canada
Prior art keywords
primer
iso
amplification
segment
pcr
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CA2778449A
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English (en)
French (fr)
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CA2778449A1 (en
Inventor
Scott Johnson
T.S. Ramasubramanian
Kathleen Engelbrecht
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Luminex Corp
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Luminex Corp
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA2778449A 2009-10-23 2010-10-22 Amplification primers with non-standard bases for increased reaction specificity Active CA2778449C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US25428109P 2009-10-23 2009-10-23
US61/254,281 2009-10-23
PCT/US2010/053773 WO2011050278A1 (en) 2009-10-23 2010-10-22 Amplification primers with non-standard bases for increased reaction specificity

Publications (2)

Publication Number Publication Date
CA2778449A1 CA2778449A1 (en) 2011-04-28
CA2778449C true CA2778449C (en) 2017-06-20

Family

ID=43898754

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2778449A Active CA2778449C (en) 2009-10-23 2010-10-22 Amplification primers with non-standard bases for increased reaction specificity

Country Status (6)

Country Link
US (1) US8440406B2 (https=)
EP (1) EP2491146B1 (https=)
JP (1) JP5938348B2 (https=)
CN (1) CN102639712A (https=)
CA (1) CA2778449C (https=)
WO (1) WO2011050278A1 (https=)

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KR102351434B1 (ko) 2013-02-07 2022-01-17 러트거즈,더스테이트유니버시티오브뉴저지 고도로 선택적인 핵산 증폭 프라이머
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CN105555971B (zh) 2013-08-09 2017-08-29 卢米耐克斯公司 用于在核酸测定中改善熔链分辨和多重性的探针
KR102323375B1 (ko) 2013-10-09 2021-11-08 플루어레센트릭 인코포레이티드 다중 프로브
CN106687589B (zh) * 2014-07-10 2021-05-07 弗洛雷森特里克公司 Dna扩增技术
US10364458B2 (en) 2014-07-16 2019-07-30 Tangen Biosciences, Inc. Isothermal methods for amplifying nucleic acid samples
EP3180449B1 (en) 2014-08-11 2019-10-09 Luminex Corporation Probes for improved melt discrimination and multiplexing in nucleic acid assays
EP3215260B1 (en) 2014-11-03 2020-01-15 Tangen Biosciences Inc. Apparatus and method for cell, spore, or virus capture and disruption
PL3298162T3 (pl) * 2015-05-18 2020-07-27 Saga Diagnostics Ab Wykrywanie docelowego kwasu nukleinowego i wariantów
CN109234456B (zh) * 2018-10-23 2023-06-09 深圳市亿立方生物技术有限公司 一种可同时检测6种呼吸道病原体的试剂盒及其应用
WO2024170770A1 (en) 2023-02-16 2024-08-22 Asecud Sa High nucleic acid sequence discrimination using a multifaceted nucleic acid molecule
WO2025023659A1 (ko) 2023-07-25 2025-01-30 주식회사 씨젠 타겟 핵산 서열에 대한 올리고뉴클레오타이드 서열의 얼라인먼트 정보를 제공하는 컴퓨터-구현 방법

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Also Published As

Publication number Publication date
EP2491146B1 (en) 2017-09-20
US8440406B2 (en) 2013-05-14
JP5938348B2 (ja) 2016-06-22
US20110097764A1 (en) 2011-04-28
CA2778449A1 (en) 2011-04-28
EP2491146A1 (en) 2012-08-29
CN102639712A (zh) 2012-08-15
JP2013507971A (ja) 2013-03-07
EP2491146A4 (en) 2013-05-01
WO2011050278A1 (en) 2011-04-28

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