JP2013507971A5 - - Google Patents
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- JP2013507971A5 JP2013507971A5 JP2012535419A JP2012535419A JP2013507971A5 JP 2013507971 A5 JP2013507971 A5 JP 2013507971A5 JP 2012535419 A JP2012535419 A JP 2012535419A JP 2012535419 A JP2012535419 A JP 2012535419A JP 2013507971 A5 JP2013507971 A5 JP 2013507971A5
- Authority
- JP
- Japan
- Prior art keywords
- primer
- segment
- pcr
- amplification
- annealing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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- 238000000034 method Methods 0.000 claims description 37
- 230000003321 amplification Effects 0.000 claims description 36
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 36
- 238000000137 annealing Methods 0.000 claims description 29
- 238000006243 chemical reaction Methods 0.000 claims description 17
- 239000002773 nucleotide Substances 0.000 claims description 16
- 125000003729 nucleotide group Chemical group 0.000 claims description 16
- 230000000295 complement effect Effects 0.000 claims description 12
- 239000002299 complementary DNA Substances 0.000 claims description 10
- 108020004999 messenger RNA Proteins 0.000 claims description 7
- 108091034117 Oligonucleotide Proteins 0.000 claims description 6
- 238000004458 analytical method Methods 0.000 claims description 6
- 238000004925 denaturation Methods 0.000 claims description 6
- 230000036425 denaturation Effects 0.000 claims description 6
- 108020004707 nucleic acids Proteins 0.000 claims description 6
- 150000007523 nucleic acids Chemical class 0.000 claims description 6
- 102000039446 nucleic acids Human genes 0.000 claims description 6
- 238000002844 melting Methods 0.000 claims description 4
- 230000008018 melting Effects 0.000 claims description 4
- 238000003753 real-time PCR Methods 0.000 claims description 4
- 230000002194 synthesizing effect Effects 0.000 claims description 4
- 238000010839 reverse transcription Methods 0.000 claims description 3
- 230000004544 DNA amplification Effects 0.000 claims description 2
- 230000004543 DNA replication Effects 0.000 claims description 2
- 108700005084 Multigene Family Proteins 0.000 claims description 2
- 108091028664 Ribonucleotide Proteins 0.000 claims description 2
- 239000005547 deoxyribonucleotide Substances 0.000 claims description 2
- 125000002637 deoxyribonucleotide group Chemical group 0.000 claims description 2
- 230000029087 digestion Effects 0.000 claims description 2
- 238000007850 in situ PCR Methods 0.000 claims description 2
- 238000007403 mPCR Methods 0.000 claims description 2
- 239000002679 microRNA Substances 0.000 claims description 2
- 238000002703 mutagenesis Methods 0.000 claims description 2
- 231100000350 mutagenesis Toxicity 0.000 claims description 2
- 108090000623 proteins and genes Proteins 0.000 claims description 2
- 238000012340 reverse transcriptase PCR Methods 0.000 claims description 2
- 239000002336 ribonucleotide Substances 0.000 claims description 2
- 125000002652 ribonucleotide group Chemical group 0.000 claims description 2
- 238000010532 solid phase synthesis reaction Methods 0.000 claims description 2
- 238000007862 touchdown PCR Methods 0.000 claims description 2
- 230000035897 transcription Effects 0.000 claims description 2
- 238000013518 transcription Methods 0.000 claims description 2
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 1
- 238000007039 two-step reaction Methods 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US25428109P | 2009-10-23 | 2009-10-23 | |
| US61/254,281 | 2009-10-23 | ||
| PCT/US2010/053773 WO2011050278A1 (en) | 2009-10-23 | 2010-10-22 | Amplification primers with non-standard bases for increased reaction specificity |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2013507971A JP2013507971A (ja) | 2013-03-07 |
| JP2013507971A5 true JP2013507971A5 (cg-RX-API-DMAC7.html) | 2014-02-13 |
| JP5938348B2 JP5938348B2 (ja) | 2016-06-22 |
Family
ID=43898754
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2012535419A Expired - Fee Related JP5938348B2 (ja) | 2009-10-23 | 2010-10-22 | 反応特異性の増加のための非標準塩基を含む増幅プライマー |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US8440406B2 (cg-RX-API-DMAC7.html) |
| EP (1) | EP2491146B1 (cg-RX-API-DMAC7.html) |
| JP (1) | JP5938348B2 (cg-RX-API-DMAC7.html) |
| CN (1) | CN102639712A (cg-RX-API-DMAC7.html) |
| CA (1) | CA2778449C (cg-RX-API-DMAC7.html) |
| WO (1) | WO2011050278A1 (cg-RX-API-DMAC7.html) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK2574681T3 (en) | 2007-03-28 | 2016-07-04 | Signal Diagnostics | System and method for high-resolution analysis of nucleic acids to detect sequence variations |
| US10669574B2 (en) | 2008-11-18 | 2020-06-02 | XCR Diagnostics, Inc. | DNA amplification technology |
| US9909159B2 (en) | 2013-02-07 | 2018-03-06 | Rutgers, The State University Of New Jersey | Highly selective nucleic acid amplification primers |
| US10174352B2 (en) * | 2013-03-14 | 2019-01-08 | Aegea Biotechnologies, Inc. | Methods for amplification of nucleic acids on solid support |
| CN107435067B (zh) | 2013-08-09 | 2021-07-09 | 卢米耐克斯公司 | 用于在核酸测定中改善熔链分辨和多重性的探针 |
| AU2014331828B2 (en) | 2013-10-09 | 2020-05-14 | Fluoresentric, Inc. | Multiplex probes |
| MX2017000391A (es) * | 2014-07-10 | 2017-07-11 | Fluoresentric Inc | Tecnologia de amplificacion de adn. |
| US10364458B2 (en) | 2014-07-16 | 2019-07-30 | Tangen Biosciences, Inc. | Isothermal methods for amplifying nucleic acid samples |
| EP3967771B1 (en) | 2014-08-11 | 2023-10-04 | Luminex Corporation | Probes for improved melt discrimination and multiplexing in nucleic acid assays |
| CN107208012B (zh) | 2014-11-03 | 2021-06-29 | 唐恩生物科学公司 | 用于细胞、孢子或病毒捕获和破碎的装置和方法 |
| MX375564B (es) * | 2015-05-18 | 2025-03-06 | Saga Diagnostics Ab | Detección de un ácido nucleico diana y variantes. |
| CN109234456B (zh) * | 2018-10-23 | 2023-06-09 | 深圳市亿立方生物技术有限公司 | 一种可同时检测6种呼吸道病原体的试剂盒及其应用 |
| EP4665870A1 (en) | 2023-02-16 | 2025-12-24 | Asecud SA | High nucleic acid sequence discrimination using a multifaceted nucleic acid molecule |
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| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US4996143A (en) | 1985-12-23 | 1991-02-26 | Syngene, Inc. | Fluorescent stokes shift probes for polynucleotide hybridization |
| US4800159A (en) | 1986-02-07 | 1989-01-24 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences |
| GB8827160D0 (en) | 1988-11-21 | 1988-12-29 | Apothekernes Lab | Detection & quantitative determination of rna & dna |
| GB8902689D0 (en) | 1989-02-07 | 1989-03-30 | Ici Plc | Assay method |
| US5856092A (en) | 1989-02-13 | 1999-01-05 | Geneco Pty Ltd | Detection of a nucleic acid sequence or a change therein |
| NZ235032A (en) | 1989-08-31 | 1993-04-28 | Dow Chemical Co | Constrained geometry complexes of titanium, zirconium or hafnium comprising a substituted cyclopentadiene ligand; use as olefin polymerisation catalyst component |
| GB8920097D0 (en) | 1989-09-06 | 1989-10-18 | Ici Plc | Amplification processes |
| US6007984A (en) | 1989-11-01 | 1999-12-28 | Zeneca Limited | Detection of DNA/RNA by fluorescence polarization |
| US5210015A (en) | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
| US5965364A (en) | 1990-10-09 | 1999-10-12 | Benner; Steven Albert | Method for selecting functional deoxyribonucleotide derivatives |
| US6140496A (en) | 1990-10-09 | 2000-10-31 | Benner; Steven Albert | Precursors for deoxyribonucleotides containing non-standard nucleosides |
| US5432272A (en) | 1990-10-09 | 1995-07-11 | Benner; Steven A. | Method for incorporating into a DNA or RNA oligonucleotide using nucleotides bearing heterocyclic bases |
| US6037120A (en) | 1995-10-12 | 2000-03-14 | Benner; Steven Albert | Recognition of oligonucleotides containing non-standard base pairs |
| DE4129653A1 (de) | 1991-09-06 | 1993-03-11 | Boehringer Mannheim Gmbh | Verfahren zum nachweis aehnlicher nukleinsaeuren |
| US5846717A (en) | 1996-01-24 | 1998-12-08 | Third Wave Technologies, Inc. | Detection of nucleic acid sequences by invader-directed cleavage |
| US20020150990A1 (en) * | 1992-02-28 | 2002-10-17 | Ian Pike | Hepatitis C virus peptides |
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| GB9507238D0 (en) | 1995-04-07 | 1995-05-31 | Isis Innovation | Detecting dna sequence variations |
| US5736330A (en) | 1995-10-11 | 1998-04-07 | Luminex Corporation | Method and compositions for flow cytometric determination of DNA sequences |
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| US5780233A (en) | 1996-06-06 | 1998-07-14 | Wisconsin Alumni Research Foundation | Artificial mismatch hybridization |
| US5866336A (en) | 1996-07-16 | 1999-02-02 | Oncor, Inc. | Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon |
| US6124092A (en) | 1996-10-04 | 2000-09-26 | The Perkin-Elmer Corporation | Multiplex polynucleotide capture methods and compositions |
| US5912147A (en) * | 1996-10-22 | 1999-06-15 | Health Research, Inc. | Rapid means of quantitating genomic instability |
| JP4185185B2 (ja) | 1997-05-08 | 2008-11-26 | 征夫 軽部 | 部分二重鎖ｄｎａを利用したｄｎａの検出方法 |
| US5928869A (en) | 1997-05-30 | 1999-07-27 | Becton, Dickinson And Company | Detection of nucleic acids by fluorescence quenching |
| EP2034031A1 (en) | 1997-10-28 | 2009-03-11 | Los Alamos National Security, LLC | DNA polymorphism identity determination using flow cytometry |
| GB9725197D0 (en) | 1997-11-29 | 1998-01-28 | Secr Defence | Detection system |
| AU3996299A (en) | 1998-05-14 | 1999-11-29 | Luminex Corporation | Diode laser based measurement apparatus |
| JP3662850B2 (ja) | 1998-06-24 | 2005-06-22 | イルミナ インコーポレイテッド | 微小球を有するアレイセンサーのデコード |
| US6528254B1 (en) | 1999-10-29 | 2003-03-04 | Stratagene | Methods for detection of a target nucleic acid sequence |
| EP1349954B1 (en) | 2000-05-19 | 2011-01-26 | Eragen Biosciences, Inc. | Materials and methods for detection of nucleic acids |
| US6511809B2 (en) | 2000-06-13 | 2003-01-28 | E. I. Du Pont De Nemours And Company | Method for the detection of an analyte by means of a nucleic acid reporter |
| AU1317502A (en) | 2000-10-14 | 2002-04-29 | Eragen Biosciences Inc | Solid support assay systems and methods utilizing non-standard bases |
| US20030175749A1 (en) | 2001-12-08 | 2003-09-18 | Jong-Yoon Chun | Annealing control primer and its uses |
| US20030194705A1 (en) | 2002-03-01 | 2003-10-16 | Schroth Gary P. | Methods of using unnatural nucleobases for decoding |
| US20040157238A1 (en) | 2002-09-20 | 2004-08-12 | Quinn John J. | Method for detection of multiple nucleic acid sequence variations |
| DE602004018270D1 (de) * | 2003-01-17 | 2009-01-22 | Eragen Biosciences Inc | Nukleinsäureamplifikation mit nichtstandardbasen |
| WO2005045073A1 (en) | 2003-11-10 | 2005-05-19 | Seegene, Inc. | Method for amplifying unknown dna sequence adjacent to known sequence |
| US20070264694A1 (en) | 2006-04-07 | 2007-11-15 | Eragen Biosciences, Inc. | Use of non-standard bases and proximity effects for gene assembly and conversion of non-standard bases to standard bases during dna synthesis |
| US20080076909A1 (en) * | 2006-06-30 | 2008-03-27 | Applera Corporation | Emulsion pcr and amplicon capture |
| CN102066574A (zh) * | 2008-06-17 | 2011-05-18 | 史蒂文·埃尔伯特·奔纳 | 利用非天然核苷酸扩增dna的过程 |
-
2010
- 2010-10-22 EP EP10825752.8A patent/EP2491146B1/en active Active
- 2010-10-22 CA CA2778449A patent/CA2778449C/en active Active
- 2010-10-22 JP JP2012535419A patent/JP5938348B2/ja not_active Expired - Fee Related
- 2010-10-22 CN CN2010800475424A patent/CN102639712A/zh active Pending
- 2010-10-22 US US12/910,113 patent/US8440406B2/en active Active
- 2010-10-22 WO PCT/US2010/053773 patent/WO2011050278A1/en not_active Ceased
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