CA2778449C - Amplification primers with non-standard bases for increased reaction specificity - Google Patents
Amplification primers with non-standard bases for increased reaction specificity Download PDFInfo
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- CA2778449C CA2778449C CA2778449A CA2778449A CA2778449C CA 2778449 C CA2778449 C CA 2778449C CA 2778449 A CA2778449 A CA 2778449A CA 2778449 A CA2778449 A CA 2778449A CA 2778449 C CA2778449 C CA 2778449C
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- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
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| DK2574681T3 (en) | 2007-03-28 | 2016-07-04 | Signal Diagnostics | System and method for high-resolution analysis of nucleic acids to detect sequence variations |
| US10669574B2 (en) | 2008-11-18 | 2020-06-02 | XCR Diagnostics, Inc. | DNA amplification technology |
| US9909159B2 (en) | 2013-02-07 | 2018-03-06 | Rutgers, The State University Of New Jersey | Highly selective nucleic acid amplification primers |
| US10174352B2 (en) * | 2013-03-14 | 2019-01-08 | Aegea Biotechnologies, Inc. | Methods for amplification of nucleic acids on solid support |
| CN107435067B (zh) | 2013-08-09 | 2021-07-09 | 卢米耐克斯公司 | 用于在核酸测定中改善熔链分辨和多重性的探针 |
| AU2014331828B2 (en) | 2013-10-09 | 2020-05-14 | Fluoresentric, Inc. | Multiplex probes |
| MX2017000391A (es) * | 2014-07-10 | 2017-07-11 | Fluoresentric Inc | Tecnologia de amplificacion de adn. |
| US10364458B2 (en) | 2014-07-16 | 2019-07-30 | Tangen Biosciences, Inc. | Isothermal methods for amplifying nucleic acid samples |
| EP3967771B1 (en) | 2014-08-11 | 2023-10-04 | Luminex Corporation | Probes for improved melt discrimination and multiplexing in nucleic acid assays |
| CN107208012B (zh) | 2014-11-03 | 2021-06-29 | 唐恩生物科学公司 | 用于细胞、孢子或病毒捕获和破碎的装置和方法 |
| MX375564B (es) * | 2015-05-18 | 2025-03-06 | Saga Diagnostics Ab | Detección de un ácido nucleico diana y variantes. |
| CN109234456B (zh) * | 2018-10-23 | 2023-06-09 | 深圳市亿立方生物技术有限公司 | 一种可同时检测6种呼吸道病原体的试剂盒及其应用 |
| EP4665870A1 (en) | 2023-02-16 | 2025-12-24 | Asecud SA | High nucleic acid sequence discrimination using a multifaceted nucleic acid molecule |
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| US4800159A (en) | 1986-02-07 | 1989-01-24 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences |
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| US5432272A (en) | 1990-10-09 | 1995-07-11 | Benner; Steven A. | Method for incorporating into a DNA or RNA oligonucleotide using nucleotides bearing heterocyclic bases |
| US6037120A (en) | 1995-10-12 | 2000-03-14 | Benner; Steven Albert | Recognition of oligonucleotides containing non-standard base pairs |
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| US20020150990A1 (en) * | 1992-02-28 | 2002-10-17 | Ian Pike | Hepatitis C virus peptides |
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| US6077668A (en) | 1993-04-15 | 2000-06-20 | University Of Rochester | Highly sensitive multimeric nucleic acid probes |
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| US20030175749A1 (en) | 2001-12-08 | 2003-09-18 | Jong-Yoon Chun | Annealing control primer and its uses |
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| DE602004018270D1 (de) * | 2003-01-17 | 2009-01-22 | Eragen Biosciences Inc | Nukleinsäureamplifikation mit nichtstandardbasen |
| WO2005045073A1 (en) | 2003-11-10 | 2005-05-19 | Seegene, Inc. | Method for amplifying unknown dna sequence adjacent to known sequence |
| US20070264694A1 (en) | 2006-04-07 | 2007-11-15 | Eragen Biosciences, Inc. | Use of non-standard bases and proximity effects for gene assembly and conversion of non-standard bases to standard bases during dna synthesis |
| US20080076909A1 (en) * | 2006-06-30 | 2008-03-27 | Applera Corporation | Emulsion pcr and amplicon capture |
| CN102066574A (zh) * | 2008-06-17 | 2011-05-18 | 史蒂文·埃尔伯特·奔纳 | 利用非天然核苷酸扩增dna的过程 |
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- 2010-10-22 WO PCT/US2010/053773 patent/WO2011050278A1/en not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| US8440406B2 (en) | 2013-05-14 |
| CA2778449A1 (en) | 2011-04-28 |
| JP5938348B2 (ja) | 2016-06-22 |
| EP2491146B1 (en) | 2017-09-20 |
| JP2013507971A (ja) | 2013-03-07 |
| EP2491146A4 (en) | 2013-05-01 |
| EP2491146A1 (en) | 2012-08-29 |
| CN102639712A (zh) | 2012-08-15 |
| US20110097764A1 (en) | 2011-04-28 |
| WO2011050278A1 (en) | 2011-04-28 |
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