CN102639712A - 用于提高反应特异性的具有非标准碱基的扩增引物 - Google Patents

用于提高反应特异性的具有非标准碱基的扩增引物 Download PDF

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Publication number
CN102639712A
CN102639712A CN2010800475424A CN201080047542A CN102639712A CN 102639712 A CN102639712 A CN 102639712A CN 2010800475424 A CN2010800475424 A CN 2010800475424A CN 201080047542 A CN201080047542 A CN 201080047542A CN 102639712 A CN102639712 A CN 102639712A
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China
Prior art keywords
primer
amplification
annealing
pcr
section
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CN2010800475424A
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English (en)
Chinese (zh)
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斯科特·约翰逊
T·S·拉玛瑟伯拉曼尼
凯瑟琳·恩格尔布雷克特
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Luminex Corp
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Luminex Corp
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN2010800475424A 2009-10-23 2010-10-22 用于提高反应特异性的具有非标准碱基的扩增引物 Pending CN102639712A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US25428109P 2009-10-23 2009-10-23
US61/254,281 2009-10-23
PCT/US2010/053773 WO2011050278A1 (en) 2009-10-23 2010-10-22 Amplification primers with non-standard bases for increased reaction specificity

Publications (1)

Publication Number Publication Date
CN102639712A true CN102639712A (zh) 2012-08-15

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CN2010800475424A Pending CN102639712A (zh) 2009-10-23 2010-10-22 用于提高反应特异性的具有非标准碱基的扩增引物

Country Status (6)

Country Link
US (1) US8440406B2 (cg-RX-API-DMAC7.html)
EP (1) EP2491146B1 (cg-RX-API-DMAC7.html)
JP (1) JP5938348B2 (cg-RX-API-DMAC7.html)
CN (1) CN102639712A (cg-RX-API-DMAC7.html)
CA (1) CA2778449C (cg-RX-API-DMAC7.html)
WO (1) WO2011050278A1 (cg-RX-API-DMAC7.html)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105102467A (zh) * 2013-02-07 2015-11-25 新泽西鲁特格斯州立大学 高度选择性核酸扩增引物
CN106661600A (zh) * 2014-07-16 2017-05-10 唐恩生物科技股份有限公司 用于扩增核酸样品的等温方法
CN106687589A (zh) * 2014-07-10 2017-05-17 弗洛雷森特里克公司 Dna扩增技术
CN109234456A (zh) * 2018-10-23 2019-01-18 深圳市亿立方生物技术有限公司 一种可同时检测6种呼吸道病原体的试剂盒及其应用

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US10174352B2 (en) * 2013-03-14 2019-01-08 Aegea Biotechnologies, Inc. Methods for amplification of nucleic acids on solid support
CN107435067B (zh) 2013-08-09 2021-07-09 卢米耐克斯公司 用于在核酸测定中改善熔链分辨和多重性的探针
AU2014331828B2 (en) 2013-10-09 2020-05-14 Fluoresentric, Inc. Multiplex probes
EP3967771B1 (en) 2014-08-11 2023-10-04 Luminex Corporation Probes for improved melt discrimination and multiplexing in nucleic acid assays
CN107208012B (zh) 2014-11-03 2021-06-29 唐恩生物科学公司 用于细胞、孢子或病毒捕获和破碎的装置和方法
MX375564B (es) * 2015-05-18 2025-03-06 Saga Diagnostics Ab Detección de un ácido nucleico diana y variantes.
EP4665870A1 (en) 2023-02-16 2025-12-24 Asecud SA High nucleic acid sequence discrimination using a multifaceted nucleic acid molecule

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Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105102467A (zh) * 2013-02-07 2015-11-25 新泽西鲁特格斯州立大学 高度选择性核酸扩增引物
US9909159B2 (en) 2013-02-07 2018-03-06 Rutgers, The State University Of New Jersey Highly selective nucleic acid amplification primers
CN105102467B (zh) * 2013-02-07 2019-01-22 新泽西鲁特格斯州立大学 高度选择性核酸扩增引物
US10815512B2 (en) 2013-02-07 2020-10-27 Rutgers, The State University Of New Jersey Highly selective nucleic acid amplification primers
US11111515B2 (en) 2013-02-07 2021-09-07 Rutgers, The State University Of New Jersey Highly selective nucleic acid amplification primers
CN106687589A (zh) * 2014-07-10 2017-05-17 弗洛雷森特里克公司 Dna扩增技术
CN106687589B (zh) * 2014-07-10 2021-05-07 弗洛雷森特里克公司 Dna扩增技术
CN106661600A (zh) * 2014-07-16 2017-05-10 唐恩生物科技股份有限公司 用于扩增核酸样品的等温方法
US11111531B2 (en) 2014-07-16 2021-09-07 Tangen Biosciences, Inc. Isothermal methods for amplifying nucleic acid samples
CN109234456A (zh) * 2018-10-23 2019-01-18 深圳市亿立方生物技术有限公司 一种可同时检测6种呼吸道病原体的试剂盒及其应用
CN109234456B (zh) * 2018-10-23 2023-06-09 深圳市亿立方生物技术有限公司 一种可同时检测6种呼吸道病原体的试剂盒及其应用

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US8440406B2 (en) 2013-05-14
CA2778449A1 (en) 2011-04-28
JP5938348B2 (ja) 2016-06-22
EP2491146B1 (en) 2017-09-20
JP2013507971A (ja) 2013-03-07
EP2491146A4 (en) 2013-05-01
EP2491146A1 (en) 2012-08-29
US20110097764A1 (en) 2011-04-28
WO2011050278A1 (en) 2011-04-28
CA2778449C (en) 2017-06-20

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Application publication date: 20120815