JP2013005803A5 - - Google Patents

Download PDF

Info

Publication number
JP2013005803A5
JP2013005803A5 JP2012179420A JP2012179420A JP2013005803A5 JP 2013005803 A5 JP2013005803 A5 JP 2013005803A5 JP 2012179420 A JP2012179420 A JP 2012179420A JP 2012179420 A JP2012179420 A JP 2012179420A JP 2013005803 A5 JP2013005803 A5 JP 2013005803A5
Authority
JP
Japan
Prior art keywords
amplification
steps
nucleic acid
target nucleic
performed simultaneously
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP2012179420A
Other languages
English (en)
Japanese (ja)
Other versions
JP5685561B2 (ja
JP2013005803A (ja
Filing date
Publication date
Application filed filed Critical
Publication of JP2013005803A publication Critical patent/JP2013005803A/ja
Publication of JP2013005803A5 publication Critical patent/JP2013005803A5/ja
Application granted granted Critical
Publication of JP5685561B2 publication Critical patent/JP5685561B2/ja
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

JP2012179420A 2000-05-19 2012-08-13 核酸の検出用物質及び検出方法 Expired - Fee Related JP5685561B2 (ja)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US20571200P 2000-05-19 2000-05-19
US60/205,712 2000-05-19
US24039800P 2000-10-14 2000-10-14
US60/240,398 2000-10-14
US28283101P 2001-04-10 2001-04-10
US60/282,831 2001-04-10

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
JP2001586611A Division JP5138141B2 (ja) 2000-05-19 2001-05-18 核酸の検出用物質及び検出方法

Publications (3)

Publication Number Publication Date
JP2013005803A JP2013005803A (ja) 2013-01-10
JP2013005803A5 true JP2013005803A5 (https=) 2014-07-31
JP5685561B2 JP5685561B2 (ja) 2015-03-18

Family

ID=27394838

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2001586611A Expired - Lifetime JP5138141B2 (ja) 2000-05-19 2001-05-18 核酸の検出用物質及び検出方法
JP2012179420A Expired - Fee Related JP5685561B2 (ja) 2000-05-19 2012-08-13 核酸の検出用物質及び検出方法

Family Applications Before (1)

Application Number Title Priority Date Filing Date
JP2001586611A Expired - Lifetime JP5138141B2 (ja) 2000-05-19 2001-05-18 核酸の検出用物質及び検出方法

Country Status (9)

Country Link
US (6) US7422850B2 (https=)
EP (1) EP1349954B1 (https=)
JP (2) JP5138141B2 (https=)
CN (1) CN1592792B (https=)
AT (1) ATE497018T1 (https=)
AU (1) AU7125401A (https=)
CA (1) CA2409309A1 (https=)
DE (1) DE60143960D1 (https=)
WO (1) WO2001090417A2 (https=)

Families Citing this family (72)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE497018T1 (de) * 2000-05-19 2011-02-15 Eragen Biosciences Inc Materialien und methoden zum nachweis von nukleinsäuren
US6977161B2 (en) 2000-10-14 2005-12-20 Eragen Biosciences, Inc. Solid support assay systems and methods utilizing non-standard bases
EP1236804A1 (en) * 2001-03-02 2002-09-04 Boehringer Mannheim Gmbh A method for determination of a nucleic acid using a control
AU2003213652A1 (en) * 2002-03-01 2003-09-16 Integrated Dna Technologies, Inc. Polynomial amplification of nucleic acids
AU2004206246B2 (en) * 2003-01-17 2009-10-01 Luminex Corporation Nucleic acid amplification using non-standard bases
WO2004090153A2 (en) 2003-04-01 2004-10-21 Eragen Biosciences,Inc. Polymerase inhibitor and method of using same
CN1280428C (zh) * 2003-05-19 2006-10-18 清华大学 一种基于微小颗粒的生物芯片系统及其应用
US7282333B2 (en) * 2003-10-20 2007-10-16 Promega Corporation Methods and compositions for nucleic acid analysis
WO2005081776A2 (en) * 2004-01-30 2005-09-09 Eragen Biosciences, Inc. Materials and methods for the detection of sars
AU2005231971A1 (en) * 2004-04-06 2005-10-20 Epigenomics Ag Method for the quantification of methylated DNA
US20060172324A1 (en) * 2005-01-28 2006-08-03 Roche Molecular Systems, Inc. Methods of genotyping using differences in melting temperature
JPWO2006095550A1 (ja) * 2005-03-04 2008-08-14 国立大学法人京都大学 Pcrプライマー、それを利用したpcr法及びpcr増幅産物、並びにpcr増幅産物を利用するデバイス及びdna−タンパク複合体
WO2006101913A2 (en) * 2005-03-18 2006-09-28 Eragen Biosciences, Inc. Methods for detecting multiple species and subspecies of neiserria
US8293472B2 (en) 2005-06-07 2012-10-23 Luminex Corporation Methods for detection and typing of nucleic acids
US20120122095A1 (en) * 2006-01-12 2012-05-17 Eragen Biosciences, Inc. Materials and methods for the detection of anthrax related toxin genes
US20070264694A1 (en) * 2006-04-07 2007-11-15 Eragen Biosciences, Inc. Use of non-standard bases and proximity effects for gene assembly and conversion of non-standard bases to standard bases during dna synthesis
US20080153097A1 (en) * 2006-11-15 2008-06-26 Eragen Biosciences, Inc. Methods and kits for detecting jak2 nucleic acid
EP2147118A4 (en) * 2007-04-27 2010-09-01 Eragen Biosciences Inc MATERIALS AND METHOD FOR DETECTING HEPATITIS C VIRUS
US9163053B2 (en) 2007-05-18 2015-10-20 Fluidigm Corporation Nucleotide analogs
US7678894B2 (en) * 2007-05-18 2010-03-16 Helicos Biosciences Corporation Nucleotide analogs
JP2009178159A (ja) * 2007-11-05 2009-08-13 Hitachi Plant Technologies Ltd 核酸配列の検出方法及び核酸配列検出基板
KR100978215B1 (ko) * 2008-02-28 2010-08-26 주식회사 인트론바이오테크놀로지 건조형태의 pcr 반응용 조성물에 기반한 pcr의 최적증폭 조건 탐색 및 설정에 활용될 수 있는 키트 및 그것의제조방법
US9249455B2 (en) * 2008-04-18 2016-02-02 Luminex Corporation Methods for detection and quantification of small RNA
EP2133434A1 (en) 2008-06-12 2009-12-16 Institut National de la Santé et de la Recherche Médicale (INSERM) Method for the detection of target nucleic acid
ES2384681T3 (es) * 2008-08-12 2012-07-10 F. Hoffmann-La Roche Ag Extensión de cebadores correctora
EP2331703A2 (en) * 2008-09-12 2011-06-15 Promega Corporation Assessing expression of endogenous and exogenous genes
US20100112565A1 (en) * 2008-11-03 2010-05-06 Life Technologies Corporation Methods, kits, and reaction mixtures for high resolution melt genotyping
US20100112557A1 (en) * 2008-11-03 2010-05-06 Applied Biosystems Inc. Method for high resolution melt genotyping
EP2391883B1 (en) 2009-01-30 2018-03-07 Micronics, Inc. Portable high gain fluorescence detection system
WO2011050278A1 (en) 2009-10-23 2011-04-28 Eragen Biosciences, Inc. Amplification primers with non-standard bases for increased reaction specificity
US8524450B2 (en) 2009-10-30 2013-09-03 Illumina, Inc. Microvessels, microparticles, and methods of manufacturing and using the same
US9758817B2 (en) * 2010-01-13 2017-09-12 Agilent Technologies, Inc. Method for identifying a nucleic acid in a sample
EP2528687B1 (en) 2010-01-29 2018-08-22 Micronics, Inc. System comprising a host instrument and a microfluidic cartridge for assays
SG184480A1 (en) * 2010-04-09 2012-11-29 Life Technologies Corp VISUALIZATION TOOL FOR qPCR GENOTYPING DATA
MX340258B (es) 2011-01-11 2016-07-01 Seegene Inc Detección de secuencias de ácido nucleico objetivo mediante ensayo de escisión y extensión del pto.
KR20130101952A (ko) * 2012-02-02 2013-09-16 주식회사 씨젠 Pto 절단과 연장-의존적 혼성화를 이용한 타겟 핵산서열의 검출
JP5976849B2 (ja) 2012-03-05 2016-08-24 シージーン アイエヌシー PTO切断及び伸長アッセイによるターゲット核酸配列でのヌクレオチド変異検出{DetectionofNucleotideVariationonTargetNucleicAcidSequencebyPTOCleavageandExtensionAssay}
AU2013202808B2 (en) 2012-07-31 2014-11-13 Gen-Probe Incorporated System and method for performing multiplex thermal melt analysis
CN104919035B (zh) 2012-12-21 2017-08-11 精密公司 便携式荧光检测系统和微测定盒
EP3549674B1 (en) 2012-12-21 2020-08-12 PerkinElmer Health Sciences, Inc. Low elasticity films for microfluidic use
WO2014100732A1 (en) 2012-12-21 2014-06-26 Micronics, Inc. Fluidic circuits and related manufacturing methods
AU2014200958B2 (en) 2013-02-25 2016-01-14 Seegene, Inc. Detection of nucleotide variation on target nucleic acid sequence
AU2013202788B2 (en) 2013-03-14 2015-10-01 Gen-Probe Incorporated Indexing signal detection module
JP6484222B2 (ja) 2013-05-07 2019-03-13 マイクロニクス, インコーポレイテッド 核酸の調製および分析のためのデバイス
JP6472788B2 (ja) 2013-05-07 2019-02-20 マイクロニクス, インコーポレイテッド 粘土鉱物およびアルカリ性溶液を使用して核酸含有試料を調製するための方法
EP2994750B1 (en) 2013-05-07 2020-08-12 PerkinElmer Health Sciences, Inc. Microfluidic devices and methods for performing serum separation and blood cross-matching
EP3022319B1 (en) 2013-07-15 2023-05-17 Seegene, Inc. Detection of target nucleic acid sequence by pto cleavage and extension-dependent immobilized oligonucleotide hybridization
EP4697022A2 (en) * 2013-08-09 2026-02-18 Luminex Corporation Probes for improved melt discrimination and multiplexing in nucleic acid assays
US11447814B2 (en) 2013-10-18 2022-09-20 Seegene, Inc. Detection of target nucleic acid sequence on solid phase by PTO cleavage and extension using HCTO assay
WO2015147370A1 (en) 2014-03-28 2015-10-01 Seegene, Inc. Detection of target nucleic acid sequences using different detection temperatures
EP4613872A3 (en) * 2014-08-11 2025-11-12 Luminex Corporation Probes for improved melt discrimination and multiplexing in nucleic acid assays
JP6834164B2 (ja) * 2016-03-29 2021-02-24 東洋紡株式会社 Pcr反応溶液の検出感度を維持する方法
EP3538668B8 (en) 2016-11-10 2025-04-23 Talis Biomedical Corporation Polynucleotides for the amplification and detection of chlamydia trachomatis
WO2018119443A1 (en) * 2016-12-23 2018-06-28 The Regents Of The University Of California Method and device for digital high resolution melt
CN106950326B (zh) * 2017-03-10 2018-05-29 武汉大学 一种化学标记结合lc-ms的方法及其在核苷酸分析中的应用
WO2018175296A2 (en) * 2017-03-20 2018-09-27 President And Fellows Of Harvard College Programmable nucleic acid synthesis cascade
KR102380264B1 (ko) 2017-08-31 2022-03-29 주식회사 씨젠 다이머-형성 프라이머 쌍을 이용한 구성요소의 성능 평가
WO2019064483A1 (ja) * 2017-09-29 2019-04-04 積水メディカル株式会社 イオン交換クロマトグラフィーを用いた一塩基置換検出方法
US11401546B2 (en) 2017-09-29 2022-08-02 Seegene, Inc. Detection of target nucleic acid sequences by PTO cleavage and extension-dependent extension assay
US20190284609A1 (en) 2018-03-14 2019-09-19 Luminex Corporation Fast, multiplex amplification of nucleic acids
AU2019255933B2 (en) 2018-04-20 2022-08-25 Seegene, Inc. . Method and apparatus for detecting a plurality of target nucleic acid sequences in sample
EP3820610A1 (en) 2018-07-12 2021-05-19 Luminex Corporation Systems and methods for performing variable sample preparation and analysis processes
CN114467027B (zh) * 2019-05-20 2025-07-15 因斯利克萨公司 用于分子检测的光触发核酸构建体和方法
KR20250047414A (ko) 2019-07-16 2025-04-03 멜리오랩스 인코포레이티드 단일-세포 기반 디지털 고해상도 용융을 위한 방법 및 장치
US11891662B2 (en) * 2019-12-02 2024-02-06 Talis Biomedical Corporation Polynucleotides for amplification and detection of human beta actin
US11047007B1 (en) 2020-03-23 2021-06-29 Talis Biomedical Corporation Polynucleotides for amplification and detection of SARS-CoV-2
CN113913497B (zh) * 2021-02-03 2024-05-28 山东舜丰生物科技有限公司 利用碱基修饰的单链核酸进行靶核酸检测的方法
WO2023043280A1 (ko) 2021-09-17 2023-03-23 주식회사 씨젠 합성 비자연 염기를 포함하는 태그 올리고뉴클레오타이드를 이용한 타겟 핵산 서열의 검출
WO2024080818A1 (ko) 2022-10-14 2024-04-18 주식회사 씨젠 상이한 tm 값을 가지는 2개의 프로브를 이용하여 샘플 내 타겟 핵산을 검출하는 방법
WO2024106890A1 (ko) 2022-11-16 2024-05-23 주식회사 씨젠 퀀칭-pto 절단 및 연장(quenching-pto cleavage and extension; q-ptoce) 어세이에 의한 타겟 핵산의 검출
EP4673561A1 (en) * 2023-02-28 2026-01-07 Seegene, Inc. Detection of target nucleic acid by lpho-assisted pto cleavage and extension assay
WO2025023659A1 (ko) 2023-07-25 2025-01-30 주식회사 씨젠 타겟 핵산 서열에 대한 올리고뉴클레오타이드 서열의 얼라인먼트 정보를 제공하는 컴퓨터-구현 방법

Family Cites Families (54)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4458066A (en) 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4996143A (en) * 1985-12-23 1991-02-26 Syngene, Inc. Fluorescent stokes shift probes for polynucleotide hybridization
US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
GB8827160D0 (en) 1988-11-21 1988-12-29 Apothekernes Lab Detection & quantitative determination of rna & dna
GB8902689D0 (en) 1989-02-07 1989-03-30 Ici Plc Assay method
US5856092A (en) 1989-02-13 1999-01-05 Geneco Pty Ltd Detection of a nucleic acid sequence or a change therein
NZ235032A (en) 1989-08-31 1993-04-28 Dow Chemical Co Constrained geometry complexes of titanium, zirconium or hafnium comprising a substituted cyclopentadiene ligand; use as olefin polymerisation catalyst component
GB8920097D0 (en) * 1989-09-06 1989-10-18 Ici Plc Amplification processes
US6007984A (en) * 1989-11-01 1999-12-28 Zeneca Limited Detection of DNA/RNA by fluorescence polarization
US5200314A (en) 1990-03-23 1993-04-06 Chiron Corporation Polynucleotide capture assay employing in vitro amplification
US5210015A (en) * 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5965364A (en) * 1990-10-09 1999-10-12 Benner; Steven Albert Method for selecting functional deoxyribonucleotide derivatives
US6037120A (en) 1995-10-12 2000-03-14 Benner; Steven Albert Recognition of oligonucleotides containing non-standard base pairs
US6140496A (en) 1990-10-09 2000-10-31 Benner; Steven Albert Precursors for deoxyribonucleotides containing non-standard nucleosides
US5432272A (en) 1990-10-09 1995-07-11 Benner; Steven A. Method for incorporating into a DNA or RNA oligonucleotide using nucleotides bearing heterocyclic bases
US5994056A (en) * 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
DE4129653A1 (de) 1991-09-06 1993-03-11 Boehringer Mannheim Gmbh Verfahren zum nachweis aehnlicher nukleinsaeuren
US5846717A (en) * 1996-01-24 1998-12-08 Third Wave Technologies, Inc. Detection of nucleic acid sequences by invader-directed cleavage
WO1994021820A1 (en) 1993-03-17 1994-09-29 City Of Hope Nucleic acid sequences having single stranded ends
US6077668A (en) * 1993-04-15 2000-06-20 University Of Rochester Highly sensitive multimeric nucleic acid probes
US5654138A (en) 1993-05-14 1997-08-05 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Von hippel-lindau (VHL) disease gene and corresponding cDNA and methods for detecting carriers of the VHL disease gene
US6156501A (en) 1993-10-26 2000-12-05 Affymetrix, Inc. Arrays of modified nucleic acid probes and methods of use
US5589329A (en) 1993-11-15 1996-12-31 Ambion, Inc. Methods and compositions for detecting base pair mismatches
EP0754240B1 (en) 1994-02-07 2003-08-20 Beckman Coulter, Inc. Ligase/polymerase-mediated genetic bit analysis of single nucleotide polymorphisms and its use in genetic analysis
US5681702A (en) * 1994-08-30 1997-10-28 Chiron Corporation Reduction of nonspecific hybridization by using novel base-pairing schemes
US5604097A (en) 1994-10-13 1997-02-18 Spectragen, Inc. Methods for sorting polynucleotides using oligonucleotide tags
US5866337A (en) 1995-03-24 1999-02-02 The Trustees Of Columbia University In The City Of New York Method to detect mutations in a nucleic acid using a hybridization-ligation procedure
GB9507238D0 (en) 1995-04-07 1995-05-31 Isis Innovation Detecting dna sequence variations
US5736330A (en) 1995-10-11 1998-04-07 Luminex Corporation Method and compositions for flow cytometric determination of DNA sequences
US5980861A (en) 1996-03-12 1999-11-09 University Of Massachusetts Chelator compositions and methods of synthesis thereof
WO1997035033A1 (en) 1996-03-19 1997-09-25 Molecular Tool, Inc. Method for determining the nucleotide sequence of a polynucleotide
US5780233A (en) 1996-06-06 1998-07-14 Wisconsin Alumni Research Foundation Artificial mismatch hybridization
US5866336A (en) * 1996-07-16 1999-02-02 Oncor, Inc. Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
US6124092A (en) 1996-10-04 2000-09-26 The Perkin-Elmer Corporation Multiplex polynucleotide capture methods and compositions
US5853993A (en) * 1996-10-21 1998-12-29 Hewlett-Packard Company Signal enhancement method and kit
JP4185185B2 (ja) * 1997-05-08 2008-11-26 征夫 軽部 部分二重鎖dnaを利用したdnaの検出方法
US5928869A (en) * 1997-05-30 1999-07-27 Becton, Dickinson And Company Detection of nucleic acids by fluorescence quenching
WO1999022030A1 (en) * 1997-10-28 1999-05-06 The Regents Of The University Of California Dna polymorphism identity determination using flow cytometry
GB9725197D0 (en) * 1997-11-29 1998-01-28 Secr Defence Detection system
CA2331896C (en) 1998-05-14 2009-09-15 Luminex Corporation Diode laser based measurement apparatus
JP3662850B2 (ja) 1998-06-24 2005-06-22 イルミナ インコーポレイテッド 微小球を有するアレイセンサーのデコード
JP2002538415A (ja) 1998-12-30 2002-11-12 デイナ−ファーバー キャンサー インスティチュート,インコーポレイテッド 変異走査アレイ、およびその使用方法
US6432642B1 (en) * 1999-01-15 2002-08-13 Pe Corporation (Ny) Binary probe and clamp composition and methods for a target hybridization detection
US6528254B1 (en) * 1999-10-29 2003-03-04 Stratagene Methods for detection of a target nucleic acid sequence
JP2001204497A (ja) 2000-01-20 2001-07-31 Japan Science & Technology Corp 蛍光共鳴エネルギー移動を利用した、dnaハイブリダイゼーション法によるdna検出方法
US6783985B1 (en) 2000-02-18 2004-08-31 Elitra Pharmaceuticals Inc. Gene disruption methodologies for drug target discovery
US6977161B2 (en) 2000-10-14 2005-12-20 Eragen Biosciences, Inc. Solid support assay systems and methods utilizing non-standard bases
ATE497018T1 (de) * 2000-05-19 2011-02-15 Eragen Biosciences Inc Materialien und methoden zum nachweis von nukleinsäuren
US6511809B2 (en) 2000-06-13 2003-01-28 E. I. Du Pont De Nemours And Company Method for the detection of an analyte by means of a nucleic acid reporter
US6843657B2 (en) * 2001-01-12 2005-01-18 Litton Systems Inc. High speed, high density interconnect system for differential and single-ended transmission applications
GB0129450D0 (en) * 2001-12-08 2002-01-30 Koninkl Philips Electronics Nv Trenched semiconductor devices and their manufacture
US20030194705A1 (en) 2002-03-01 2003-10-16 Schroth Gary P. Methods of using unnatural nucleobases for decoding

Similar Documents

Publication Publication Date Title
JP2013005803A5 (https=)
JP2017514877A5 (https=)
JP2016525888A5 (https=)
JP2012143233A5 (https=)
JP2013033060A5 (https=)
JP2013541578A5 (https=)
JP2011102971A5 (https=)
JP2015520667A5 (https=)
JP2015503422A5 (https=)
UA118090C2 (uk) Спосіб інтегрування послідовності нуклеїнової кислоти, що представляє інтерес, у ген fad2 у клітині сої та специфічний для локусу fad2 білок, що зв'язується, здатний індукувати спрямований розрив
JP2013521563A5 (https=)
JP2011093937A5 (https=)
JP2012098988A5 (https=)
GB201219137D0 (en) Direct nucleic acid amplification kit, reagent and method
EP2971123A4 (en) Nucleic acid amplification
JP2012098270A5 (https=)
JP2015501646A5 (https=)
IL263870A (en) Improvements in or relating to nucleic acid amplification processes
BR112014006668A2 (pt) sistema liberador de ácaro predador fitoseídeo, utilização de um ácaro anfitrião, método produzir um sistema liberador de ácaros predadores fitoseídeos, e utilização do sistema liberador de ácaro predador fitoseídeo
JP2011233516A5 (https=)
WO2015112948A3 (en) Methods for determining a nucleotide sequence
JP2014507463A5 (https=)
EP3259373A4 (en) Small nucleic acid quantification using split cycle amplification
JP2011082173A5 (https=)
CA2869033C (en) Nucleic acids for nucleic acid amplification