JP2012520933A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2012520933A5 JP2012520933A5 JP2012500896A JP2012500896A JP2012520933A5 JP 2012520933 A5 JP2012520933 A5 JP 2012520933A5 JP 2012500896 A JP2012500896 A JP 2012500896A JP 2012500896 A JP2012500896 A JP 2012500896A JP 2012520933 A5 JP2012520933 A5 JP 2012520933A5
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- alkyl
- aryl
- amount
- compound
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 150000007523 nucleic acids Chemical class 0.000 claims 26
- 108020004707 nucleic acids Proteins 0.000 claims 24
- 102000039446 nucleic acids Human genes 0.000 claims 24
- 150000001875 compounds Chemical class 0.000 claims 16
- 238000000034 method Methods 0.000 claims 14
- 230000003321 amplification Effects 0.000 claims 10
- 125000003118 aryl group Chemical group 0.000 claims 10
- 238000003199 nucleic acid amplification method Methods 0.000 claims 10
- 125000000217 alkyl group Chemical group 0.000 claims 9
- 125000004429 atom Chemical group 0.000 claims 7
- 125000000753 cycloalkyl group Chemical group 0.000 claims 6
- 125000005843 halogen group Chemical group 0.000 claims 6
- 125000001072 heteroaryl group Chemical group 0.000 claims 6
- 125000001495 ethyl group Chemical group [H]C([H])([H])C([H])([H])* 0.000 claims 5
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 claims 5
- 125000001436 propyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])[H] 0.000 claims 5
- 239000011541 reaction mixture Substances 0.000 claims 5
- 125000004169 (C1-C6) alkyl group Chemical group 0.000 claims 4
- 125000004209 (C1-C8) alkyl group Chemical group 0.000 claims 4
- 125000002877 alkyl aryl group Chemical group 0.000 claims 4
- -1 amino, hydroxy Chemical group 0.000 claims 4
- 125000005605 benzo group Chemical group 0.000 claims 4
- 239000000203 mixture Substances 0.000 claims 4
- 125000000094 2-phenylethyl group Chemical group [H]C1=C([H])C([H])=C(C([H])=C1[H])C([H])([H])C([H])([H])* 0.000 claims 3
- 125000006201 3-phenylpropyl group Chemical group [H]C1=C([H])C([H])=C(C([H])=C1[H])C([H])([H])C([H])([H])C([H])([H])* 0.000 claims 3
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 claims 3
- 125000001797 benzyl group Chemical group [H]C1=C([H])C([H])=C(C([H])=C1[H])C([H])([H])* 0.000 claims 3
- 125000000623 heterocyclic group Chemical group 0.000 claims 3
- 125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 claims 3
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 2
- 125000000484 butyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 claims 2
- XHXFXVLFKHQFAL-UHFFFAOYSA-N phosphoryl trichloride Chemical compound ClP(Cl)(Cl)=O XHXFXVLFKHQFAL-UHFFFAOYSA-N 0.000 claims 2
- 238000003752 polymerase chain reaction Methods 0.000 claims 2
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 claims 2
- 108020004414 DNA Proteins 0.000 claims 1
- 102000053602 DNA Human genes 0.000 claims 1
- 102000004190 Enzymes Human genes 0.000 claims 1
- 108090000790 Enzymes Proteins 0.000 claims 1
- 238000000338 in vitro Methods 0.000 claims 1
- 238000001727 in vivo Methods 0.000 claims 1
- 230000001678 irradiating effect Effects 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- 238000012544 monitoring process Methods 0.000 claims 1
- 150000003839 salts Chemical class 0.000 claims 1
- 238000005382 thermal cycling Methods 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US16056609P | 2009-03-16 | 2009-03-16 | |
| US61/160,566 | 2009-03-16 | ||
| PCT/US2010/027514 WO2010107816A2 (en) | 2009-03-16 | 2010-03-16 | Nucleic acid binding dyes and uses therefor |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2012520933A JP2012520933A (ja) | 2012-09-10 |
| JP2012520933A5 true JP2012520933A5 (enExample) | 2013-05-09 |
| JP5746135B2 JP5746135B2 (ja) | 2015-07-08 |
Family
ID=42731028
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2012500896A Active JP5746135B2 (ja) | 2009-03-16 | 2010-03-16 | 核酸結合色素およびその使用 |
Country Status (4)
| Country | Link |
|---|---|
| US (3) | US8598198B2 (enExample) |
| EP (1) | EP2408863B1 (enExample) |
| JP (1) | JP5746135B2 (enExample) |
| WO (1) | WO2010107816A2 (enExample) |
Families Citing this family (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP5627574B2 (ja) | 2008-06-03 | 2014-11-19 | インターミューン， インコーポレイテッド | 炎症性および線維性疾患を治療するための化合物および方法 |
| JP5746135B2 (ja) | 2009-03-16 | 2015-07-08 | プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ | 核酸結合色素およびその使用 |
| JP5730311B2 (ja) * | 2009-08-31 | 2015-06-10 | プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ | 反応性シアニン化合物 |
| WO2012083235A1 (en) | 2010-12-16 | 2012-06-21 | Bio-Rad Laboratories, Inc. | Universal reference dye for quantitative amplification |
| WO2014005125A2 (en) | 2012-06-29 | 2014-01-03 | Biotium, Inc. | Fluorescent compounds and uses thereof |
| US9034606B2 (en) | 2012-08-23 | 2015-05-19 | New England Biolabs, Inc. | Detection of an amplification reaction product using pH-sensitive dyes |
| AR092742A1 (es) | 2012-10-02 | 2015-04-29 | Intermune Inc | Piridinonas antifibroticas |
| US10557853B2 (en) * | 2012-12-31 | 2020-02-11 | Yeda Research And Development Co. Ltd. | Molecular sensor and methods of use thereof |
| US10233195B2 (en) | 2014-04-02 | 2019-03-19 | Intermune, Inc. | Anti-fibrotic pyridinones |
| CN109642248B (zh) * | 2016-03-17 | 2022-09-02 | 珀金埃尔默健康科学有限责任公司 | 液体样品中细胞的检测 |
| CN105753781B (zh) * | 2016-04-06 | 2018-01-26 | 陈科 | 一种4‑溴甲基喹啉酮的绿色合成方法 |
| US20180188204A1 (en) * | 2017-01-01 | 2018-07-05 | Sylvester Tumusiime | Use of charged quinine sulfate or other precursors or derivatives of quinine alkaloids in visualization of nucleic acids |
| JP7711058B2 (ja) | 2019-11-25 | 2025-07-22 | エーエムペー・バイオテック・ゲーエムベーハー | 固体表面に結合させた親和性リガンドを使用する核酸の分離及び単離 |
| JP7029841B1 (ja) | 2020-08-21 | 2022-03-04 | 国立大学法人東北大学 | Ｒｎａ検出用蛍光色素及びその使用方法 |
| WO2022051405A1 (en) | 2020-09-02 | 2022-03-10 | Promega Corporation | Nucleic acid modifying reagents and uses thereof |
| JP2022132057A (ja) | 2021-02-26 | 2022-09-07 | 保土谷化学工業株式会社 | キサンテン色素、該色素を含有する着色組成物、カラーフィルター用着色剤およびカラーフィルター |
| US20230366045A1 (en) | 2022-03-01 | 2023-11-16 | Promega Corporation | Nucleic acid crosslinking reagents and uses thereof |
| CN120129825A (zh) | 2022-10-14 | 2025-06-10 | 10X基因组学有限公司 | 用于评估生物样品质量的方法、组合物和系统 |
| CN117164577B (zh) * | 2023-08-10 | 2024-11-15 | 郑州玛特瑞斯生物科技有限公司 | 一种dsDNA定量用荧光化合物、试剂盒、制备方法 |
| WO2025187095A1 (ja) * | 2024-03-04 | 2025-09-12 | 国立大学法人東北大学 | 化合物、蛍光色素、化合物の使用、試料中のｒｎａを検出するための方法、キット、エンベロープを有するウイルスに対する感染を検出するための方法 |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BE440738A (enExample) | 1940-03-05 | |||
| US2269234A (en) | 1940-10-18 | 1942-01-06 | Eastman Kodak Co | Polymethine dye intermediates |
| US2398999A (en) * | 1942-09-01 | 1946-04-23 | Eastman Kodak Co | Polymethine dyes |
| US4665024A (en) | 1984-10-01 | 1987-05-12 | Becton, Dickinson And Company | Fluorescent gram stain |
| US4965188A (en) | 1986-08-22 | 1990-10-23 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US5321130A (en) * | 1992-02-10 | 1994-06-14 | Molecular Probes, Inc. | Unsymmetrical cyanine dyes with a cationic side chain |
| JPH05287266A (ja) * | 1992-04-09 | 1993-11-02 | Hitachi Chem Co Ltd | 蛍光標識用色素、蛍光標識用色素で標識された生物由来物質、及びそれらを含有する試薬 |
| US5658751A (en) | 1993-04-13 | 1997-08-19 | Molecular Probes, Inc. | Substituted unsymmetrical cyanine dyes with selected permeability |
| US5534416A (en) * | 1993-04-13 | 1996-07-09 | Molecular Probes, Inc. | Fluorescent viability assay using cyclic-substituted unsymmetrical cyanine dyes |
| JP3918369B2 (ja) * | 1999-03-05 | 2007-05-23 | コニカミノルタホールディングス株式会社 | ハロゲン化銀乳剤、ハロゲン化銀感光材料、熱現像感光材料及びこれを用いた画像記録方法及び画像形成方法、並びに熱現像感光材料の製造方法 |
| JP5746135B2 (ja) | 2009-03-16 | 2015-07-08 | プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ | 核酸結合色素およびその使用 |
-
2010
- 2010-03-16 JP JP2012500896A patent/JP5746135B2/ja active Active
- 2010-03-16 US US12/725,251 patent/US8598198B2/en active Active
- 2010-03-16 WO PCT/US2010/027514 patent/WO2010107816A2/en not_active Ceased
- 2010-03-16 EP EP10709944.2A patent/EP2408863B1/en active Active
-
2013
- 2013-11-26 US US14/090,782 patent/US9206474B2/en active Active
-
2015
- 2015-12-07 US US14/960,589 patent/US9458499B2/en active Active
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| ES2638853T3 (es) | Amplificación isotérmica de ácido nucleico | |
| WO2013177429A4 (en) | Extreme pcr | |
| Zhang et al. | Ultrasensitive quantification of mature microRNAs by real-time PCR based on ligation of a ribonucleotide-modified DNA probe | |
| DK2134861T3 (da) | Fremgangsmåde for smelteanalyse af nukleinsyrer | |
| WO2008051928A3 (en) | Target-oriented whole genome amplification of nucliec acids | |
| JP2010207220A5 (enExample) | ||
| CN104093856A (zh) | 检测核酸合成和/或扩增的方法 | |
| IL316759A (en) | Preparations and methods for identifying biological contaminants | |
| NZ708935A (en) | Photoactivated chemical bleaching of dyes | |
| RU2016116333A (ru) | Определение нуклеиновых кислот путем амплификации, основанной на встраивании в цепь | |
| WO2008017948A3 (en) | Method for assaying nucleic acids by fluorescence | |
| BR112012030813A2 (pt) | "métodos e kits para detecção in situ de sequências de nucleotídeo" | |
| JP2010506592A5 (enExample) | ||
| WO2014003583A4 (en) | Detection of pathogens | |
| WO2010072822A3 (de) | Präparation und amplifikation von nukleinsäuren mittels magnetischer partikel | |
| Tian et al. | Sensitive analysis of DNA methyltransferase based on a hairpin-shaped DNAzyme | |
| Liu et al. | Label-free and signal-on electrochemiluminescence aptasensor for ATP based on target-induced linkage of split aptamer fragments by using [Ru (phen) 3] 2+ intercalated into double-strand DNA as a probe | |
| ATE525479T1 (de) | Spezifische oligonukleotidsequenzen von neisseria gonorrhoeae | |
| NZ732886A (en) | Amplification and detection of nucleic acids in a biological sample | |
| Li et al. | Integrating optical tweezers with up-converting luminescence: a non-amplification analytical platform for quantitative detection of microRNA-21 sequences | |
| García-Beltrán et al. | A coumarinylaldoxime as a specific sensor for Cu2+ and its biological application | |
| WO2006131892A3 (en) | Amplification of nucleic acids with magnetic detection | |
| WO2011025329A3 (en) | Detecting methods of dna amplification using new intercalating agent | |
| WO2014101276A3 (zh) | 确定核酸样本的两个预定位点是否具有已知突变的方法、试剂盒以及引物 | |
| WO2008066979A3 (en) | Methods for rapid, single-step strand displacement amplification of nucleic acids |