JP2012515161A - 負荷電ポリマーによる生体分子の沈殿 - Google Patents

負荷電ポリマーによる生体分子の沈殿 Download PDF

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Publication number
JP2012515161A
JP2012515161A JP2011545324A JP2011545324A JP2012515161A JP 2012515161 A JP2012515161 A JP 2012515161A JP 2011545324 A JP2011545324 A JP 2011545324A JP 2011545324 A JP2011545324 A JP 2011545324A JP 2012515161 A JP2012515161 A JP 2012515161A
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JP
Japan
Prior art keywords
polymer
biomolecule
antibody
precipitate
aqueous
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2011545324A
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English (en)
Japanese (ja)
Other versions
JP2012515161A5 (enExample
Inventor
アルスティネ，ヤメス ファン
シャナガール，ヤミル
フヨルス，ロルフ
ラクキ，カロル
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cytiva Sweden AB
Original Assignee
Amersham Bioscience AB
GE Healthcare Bio Sciences Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amersham Bioscience AB, GE Healthcare Bio Sciences Corp filed Critical Amersham Bioscience AB
Publication of JP2012515161A publication Critical patent/JP2012515161A/ja
Publication of JP2012515161A5 publication Critical patent/JP2012515161A5/ja
Pending legal-status Critical Current

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/395—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum
    • A61K39/39591—Stabilisation, fragmentation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/30—Extraction; Separation; Purification by precipitation
    • C07K1/32—Extraction; Separation; Purification by precipitation as complexes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/32—Bonded phase chromatography
    • B01D15/325—Reversed phase
    • B01D15/327—Reversed phase with hydrophobic interaction
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361—Ion-exchange
    • B01D15/362—Cation-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3804—Affinity chromatography
    • B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G or L chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3847—Multimodal interactions

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Epidemiology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Public Health (AREA)
  • Engineering & Computer Science (AREA)
  • Peptides Or Proteins (AREA)
  • Medicinal Preparation (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
JP2011545324A 2009-01-13 2010-01-11 負荷電ポリマーによる生体分子の沈殿 Pending JP2012515161A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
SE0900032 2009-01-13
SE0900032-4 2009-01-13
PCT/SE2010/050017 WO2010082894A1 (en) 2009-01-13 2010-01-11 Precipitation of biomolecules with negatively charged polymers

Publications (2)

Publication Number Publication Date
JP2012515161A true JP2012515161A (ja) 2012-07-05
JP2012515161A5 JP2012515161A5 (enExample) 2013-02-21

Family

ID=42339999

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2011545324A Pending JP2012515161A (ja) 2009-01-13 2010-01-11 負荷電ポリマーによる生体分子の沈殿

Country Status (7)

Country Link
US (1) US20120022239A1 (enExample)
EP (1) EP2376523A4 (enExample)
JP (1) JP2012515161A (enExample)
CN (1) CN102272146A (enExample)
AU (1) AU2010205007A1 (enExample)
CA (1) CA2748809A1 (enExample)
WO (1) WO2010082894A1 (enExample)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2902799B1 (fr) 2006-06-27 2012-10-26 Millipore Corp Procede et unite de preparation d'un echantillon pour l'analyse microbiologique d'un liquide
US8362217B2 (en) 2006-12-21 2013-01-29 Emd Millipore Corporation Purification of proteins
WO2008079280A1 (en) 2006-12-21 2008-07-03 Millipore Corporation Purification of proteins
US8569464B2 (en) 2006-12-21 2013-10-29 Emd Millipore Corporation Purification of proteins
US20120177610A1 (en) 2007-09-19 2012-07-12 Kieu Hoang Manufacturing and Purification Processes of Complex Protein found in Fraction IV to make a separated Apo, Transferrin , and Alpha 1 Anti strepsin (A1AT) or A combined Transferrin / Apo/Human Albumin/A1AT and all new found proteins
WO2009151514A1 (en) 2008-06-11 2009-12-17 Millipore Corporation Stirred tank bioreactor
US20100190963A1 (en) 2008-12-16 2010-07-29 Millipore Corporation Stirred Tank Reactor And Method
EP2571903B1 (en) 2010-05-17 2019-09-04 EMD Millipore Corporation Stimulus responsive polymers for the purification of biomolecules
WO2013021143A1 (fr) * 2011-08-10 2013-02-14 Adocia Solution injectable d'au moins une insuline basale
WO2013039449A1 (en) * 2011-09-16 2013-03-21 Ge Healthcare Bio-Sciences Ab Plasma protein fractionation by sequential polyacid precipitation
WO2013075740A1 (en) 2011-11-23 2013-05-30 Sanofi Antibody purification method
AR089231A1 (es) 2011-12-15 2014-08-06 Amgen Inc Metodo de floculacion
TW201335181A (zh) * 2012-01-31 2013-09-01 Kieu Hoang ５５種新發現的蛋白質之序列及其應用
CN103293299B (zh) * 2012-02-24 2016-06-08 珀金埃尔默医学诊断产品（上海）有限公司 拓宽双抗体夹心免疫检测浓度范围的方法及其试剂盒
TWI631132B (zh) 2013-05-06 2018-08-01 賽諾菲公司 用於純化抗體之連續多步驟方法
CN105980846B (zh) 2014-02-21 2018-12-07 宝生物工程美国有限公司 包括聚(酸)膜分离基质的旋转柱及其制造和使用的方法
GB2539420B (en) * 2015-06-16 2021-01-13 Cytiva Sweden Ab Determination of chromatography conditions
EP3546475A1 (en) 2018-03-27 2019-10-02 Sanofi Full flow-through process for purifying recombinant proteins
EP3837035B1 (en) 2018-08-16 2026-05-20 Emd Millipore Corp Closed bioprocessing device
CN114574457B (zh) * 2022-01-25 2024-07-23 华东理工大学 一种快速分离纯化蛋白质的方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080193891A1 (en) * 2005-01-25 2008-08-14 Saint-Gobain Industriekeramik R0Dental Gmbh Supporting Device For Articles to be Fired That Has an Elastic Support Fixing
WO2008156409A1 (en) * 2007-06-19 2008-12-24 Ge Healthcare Bio-Sciences Ab Separation method using polymer multi phase systems

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE348942B (enExample) * 1970-06-02 1972-09-18 Statens Bakteriologiska Labor
US5976382A (en) * 1996-07-10 1999-11-02 Ligochem, Inc. Removal of proteins from aqueuos media by precipitation
US8362217B2 (en) * 2006-12-21 2013-01-29 Emd Millipore Corporation Purification of proteins
JP5361738B2 (ja) * 2007-01-22 2013-12-04 ジェネンテック， インコーポレイテッド 多価電解質沈殿物及びタンパク質の精製
WO2008100578A2 (en) * 2007-02-14 2008-08-21 Amgen Inc. Method of isolating antibodies by precipitation

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080193891A1 (en) * 2005-01-25 2008-08-14 Saint-Gobain Industriekeramik R0Dental Gmbh Supporting Device For Articles to be Fired That Has an Elastic Support Fixing
WO2008156409A1 (en) * 2007-06-19 2008-12-24 Ge Healthcare Bio-Sciences Ab Separation method using polymer multi phase systems

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
JPN6014022513; Ｂｉｏｔｅｃｈｎｏｌｏｇｙ　ａｎｄ　Ｂｉｏｅｎｇｉｎｅｅｒｉｎｇ Ｖｏｌ．１０２，　Ｎｏ．４, 2008, ｐ．１１４１-１１５１ *
JPN6014022516; ＧＥ　Ｈｅａｌｔｈｃａｒｅ　Ｄａｔａ　Ｆｉｌｅ　１１-００３５-４５　ＡＡ，　Ｍｕｌｔｉｍｏｄａｌ　ｍｅｄｉａ，　Ｃａｐｔｏ　ＭＭＣ , 2005, ｐ．１-６ *

Also Published As

Publication number Publication date
AU2010205007A1 (en) 2011-07-07
CA2748809A1 (en) 2010-07-22
US20120022239A1 (en) 2012-01-26
EP2376523A1 (en) 2011-10-19
CN102272146A (zh) 2011-12-07
WO2010082894A1 (en) 2010-07-22
EP2376523A4 (en) 2012-11-28

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