JP2012509084A - ポリヌクレオチドアセンブリに関する組成物及び方法 - Google Patents

ポリヌクレオチドアセンブリに関する組成物及び方法 Download PDF

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JP2012509084A
JP2012509084A JP2011537596A JP2011537596A JP2012509084A JP 2012509084 A JP2012509084 A JP 2012509084A JP 2011537596 A JP2011537596 A JP 2011537596A JP 2011537596 A JP2011537596 A JP 2011537596A JP 2012509084 A JP2012509084 A JP 2012509084A
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nucleic acid
annealable linker
restriction site
restriction
linker sequence
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セルベル ザクフ
ロウエ ライモンド
エー. ウベルサク ジェエフフレイ
エス. チャンドラン スニル
ジェエデドイアフ デアン エリク
エム. プルアトト ダルレン
トシキ タケオカ ケンネトフ
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アムイリス, インコーポレイテッド
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1093General methods of preparing gene libraries, not provided for in other subgroups
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
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    • C40COMBINATORIAL TECHNOLOGY
    • C40BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00Libraries per se, e.g. arrays, mixtures
    • C40B40/04Libraries containing only organic compounds
    • C40B40/06Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • CCHEMISTRY; METALLURGY
    • C40COMBINATORIAL TECHNOLOGY
    • C40BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06Biochemical methods, e.g. using enzymes or whole viable microorganisms

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  • General Chemical & Material Sciences (AREA)
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  • Crystallography & Structural Chemistry (AREA)
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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
JP2011537596A 2008-11-19 2009-11-19 ポリヌクレオチドアセンブリに関する組成物及び方法 Pending JP2012509084A (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US11610908P 2008-11-19 2008-11-19
US61/116,109 2008-11-19
US16223009P 2009-03-20 2009-03-20
US61/162,230 2009-03-20
PCT/US2009/065048 WO2010059763A1 (en) 2008-11-19 2009-11-19 Compositions and methods for the assembly of polynucleotides

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JP2012509084A true JP2012509084A (ja) 2012-04-19
JP2012509084A5 JP2012509084A5 (https=) 2012-10-11

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US (3) US8221982B2 (https=)
EP (1) EP2358875B1 (https=)
JP (1) JP2012509084A (https=)
KR (1) KR101794298B1 (https=)
CN (1) CN102282255B (https=)
AU (1) AU2009316660B2 (https=)
BR (1) BRPI0922187B1 (https=)
CA (1) CA2744153C (https=)
MX (1) MX2011005195A (https=)
SG (1) SG171760A1 (https=)
WO (1) WO2010059763A1 (https=)
ZA (1) ZA201103637B (https=)

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JP2015521850A (ja) * 2012-06-29 2015-08-03 マサチューセッツ インスティテュート オブ テクノロジー 超並列コンビナトリアル遺伝学
JP2021151200A (ja) * 2020-03-24 2021-09-30 国立大学法人 東京大学 連結dnaの製造方法及びそれに用いるためのベクターの組み合わせ

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US8332160B1 (en) 2011-11-17 2012-12-11 Amyris Biotechnologies, Inc. Systems and methods for engineering nucleic acid constructs using scoring techniques
AU2013203380A1 (en) * 2012-01-13 2013-08-01 Chromatin, Inc. Engineering plants with rate limiting farnesene metabolic genes
CN104204205B (zh) * 2012-03-27 2017-06-27 帝斯曼知识产权资产管理有限公司 克隆方法
US9976162B2 (en) 2012-12-10 2018-05-22 Agilent Technologies, Inc. Pairing code directed assembly
MX364748B (es) 2013-03-15 2019-05-06 Amyris Inc Uso de fosfoquetolasa y fosfotransacetilasa para la produccion de compuestos derivados de acetil-coenzima a.
CA2909733C (en) 2013-04-18 2023-10-17 Fondazione Telethon Effective delivery of large genes by dual aav vectors
MX2016007797A (es) 2013-12-19 2016-09-07 Amyris Inc Metodos para integracion genomica.
WO2015120403A1 (en) 2014-02-08 2015-08-13 The Regents Of The University Of Colorado, A Body Corporate Multiplexed linking pcr
US11898141B2 (en) * 2014-05-27 2024-02-13 The Broad Institute, Inc. High-throughput assembly of genetic elements
JP2018501814A (ja) 2014-11-11 2018-01-25 クララ フーズ カンパニー 卵白タンパク質産生のための方法および組成物
HK1245346A1 (zh) 2014-12-05 2018-08-24 Amyris, Inc. 多核苷酸的高通量测序
RS63416B1 (sr) * 2015-03-03 2022-08-31 Fond Telethon Sistem višestrukih vektora i njegove primene
JP6698708B2 (ja) 2015-06-09 2020-05-27 ライフ テクノロジーズ コーポレーション 分子タグ付けのための方法、システム、組成物、キット、装置、及びコンピュータ可読媒体
WO2017024173A1 (en) 2015-08-04 2017-02-09 The Regents Of The University Of Colorado Multiplexed binary assembly and quantitative tracking of bacterial populations
WO2017075538A1 (en) 2015-10-29 2017-05-04 Amyris, Inc. Compositions and methods for production of myrcene
CN106811459B (zh) * 2015-11-27 2019-09-27 清华大学 一种利用短链引物阻遏提高dna克隆及组装效率的方法
JP6821598B2 (ja) 2015-12-07 2021-01-27 ザイマージェン インコーポレイテッド Corynebacterium glutamicum由来のプロモーター
US11208649B2 (en) 2015-12-07 2021-12-28 Zymergen Inc. HTP genomic engineering platform
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JP2019519241A (ja) 2016-06-30 2019-07-11 ザイマージェン インコーポレイテッド グルコース透過酵素ライブラリーを生成するための方法およびその使用
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Cited By (4)

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Publication number Priority date Publication date Assignee Title
JP2015521850A (ja) * 2012-06-29 2015-08-03 マサチューセッツ インスティテュート オブ テクノロジー 超並列コンビナトリアル遺伝学
JP2021151200A (ja) * 2020-03-24 2021-09-30 国立大学法人 東京大学 連結dnaの製造方法及びそれに用いるためのベクターの組み合わせ
WO2021192596A1 (ja) * 2020-03-24 2021-09-30 国立大学法人東京大学 連結dnaの製造方法及びそれに用いるためのベクターの組み合わせ
JP7473953B2 (ja) 2020-03-24 2024-04-24 国立大学法人 東京大学 連結dnaの製造方法及びそれに用いるためのベクターの組み合わせ

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US8110360B2 (en) 2012-02-07
US20100136633A1 (en) 2010-06-03
SG171760A1 (en) 2011-07-28
CA2744153A1 (en) 2010-05-27
ZA201103637B (en) 2012-08-29
US8221982B2 (en) 2012-07-17
CA2744153C (en) 2020-07-07
KR20110102351A (ko) 2011-09-16
US8546136B2 (en) 2013-10-01
AU2009316660A1 (en) 2011-07-07
BRPI0922187A2 (pt) 2018-10-23
BRPI0922187B1 (pt) 2021-05-04
EP2358875B1 (en) 2015-08-12
US20120245056A1 (en) 2012-09-27
AU2009316660B2 (en) 2015-01-29
MX2011005195A (es) 2011-09-01
US20100124768A1 (en) 2010-05-20
WO2010059763A1 (en) 2010-05-27
CN102282255B (zh) 2014-05-07
KR101794298B1 (ko) 2017-11-20
EP2358875A1 (en) 2011-08-24
CN102282255A (zh) 2011-12-14

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