CN102282255B - 多核苷酸装配的组合物和方法 - Google Patents

多核苷酸装配的组合物和方法 Download PDF

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CN102282255B
CN102282255B CN200980154897.0A CN200980154897A CN102282255B CN 102282255 B CN102282255 B CN 102282255B CN 200980154897 A CN200980154897 A CN 200980154897A CN 102282255 B CN102282255 B CN 102282255B
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nucleic acid
sequence
restriction enzyme
dna
acid molecule
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CN102282255A (zh
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扎克·塞贝
雷蒙德·洛
杰弗里·A·尤伯萨克斯
苏尼尔·S·钱德兰
埃里克·杰迪戴亚·迪安
达伦·M·普拉特
肯尼思·俊树·竹冈
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Amyris Inc
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
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    • C40—COMBINATORIAL TECHNOLOGY
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    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
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    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms

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CN200980154897.0A 2008-11-19 2009-11-19 多核苷酸装配的组合物和方法 Expired - Fee Related CN102282255B (zh)

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US11610908P 2008-11-19 2008-11-19
US61/116,109 2008-11-19
US16223009P 2009-03-20 2009-03-20
US61/162,230 2009-03-20
PCT/US2009/065048 WO2010059763A1 (en) 2008-11-19 2009-11-19 Compositions and methods for the assembly of polynucleotides

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CN102282255A CN102282255A (zh) 2011-12-14
CN102282255B true CN102282255B (zh) 2014-05-07

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US (3) US8221982B2 (https=)
EP (1) EP2358875B1 (https=)
JP (1) JP2012509084A (https=)
KR (1) KR101794298B1 (https=)
CN (1) CN102282255B (https=)
AU (1) AU2009316660B2 (https=)
BR (1) BRPI0922187B1 (https=)
CA (1) CA2744153C (https=)
MX (1) MX2011005195A (https=)
SG (1) SG171760A1 (https=)
WO (1) WO2010059763A1 (https=)
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Families Citing this family (62)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010059763A1 (en) * 2008-11-19 2010-05-27 Amyris Biotechnologies, Inc. Compositions and methods for the assembly of polynucleotides
US7919605B1 (en) 2010-08-30 2011-04-05 Amyris, Inc. Nucleic acids, compositions and methods for the excision of target nucleic acids
KR102068107B1 (ko) 2011-04-27 2020-01-20 아미리스 인코퍼레이티드 게놈 변형 방법
CN104039974B (zh) 2011-11-09 2016-10-12 阿迈瑞斯公司 乙酰辅酶a衍生的类异戊二烯的生产
US8332160B1 (en) 2011-11-17 2012-12-11 Amyris Biotechnologies, Inc. Systems and methods for engineering nucleic acid constructs using scoring techniques
AU2013203380A1 (en) * 2012-01-13 2013-08-01 Chromatin, Inc. Engineering plants with rate limiting farnesene metabolic genes
CN104204205B (zh) * 2012-03-27 2017-06-27 帝斯曼知识产权资产管理有限公司 克隆方法
SG11201408736SA (en) * 2012-06-29 2015-03-30 Massachusetts Inst Technology Massively parallel combinatorial genetics
US9976162B2 (en) 2012-12-10 2018-05-22 Agilent Technologies, Inc. Pairing code directed assembly
MX364748B (es) 2013-03-15 2019-05-06 Amyris Inc Uso de fosfoquetolasa y fosfotransacetilasa para la produccion de compuestos derivados de acetil-coenzima a.
CA2909733C (en) 2013-04-18 2023-10-17 Fondazione Telethon Effective delivery of large genes by dual aav vectors
MX2016007797A (es) 2013-12-19 2016-09-07 Amyris Inc Metodos para integracion genomica.
WO2015120403A1 (en) 2014-02-08 2015-08-13 The Regents Of The University Of Colorado, A Body Corporate Multiplexed linking pcr
US11898141B2 (en) * 2014-05-27 2024-02-13 The Broad Institute, Inc. High-throughput assembly of genetic elements
JP2018501814A (ja) 2014-11-11 2018-01-25 クララ フーズ カンパニー 卵白タンパク質産生のための方法および組成物
HK1245346A1 (zh) 2014-12-05 2018-08-24 Amyris, Inc. 多核苷酸的高通量测序
RS63416B1 (sr) * 2015-03-03 2022-08-31 Fond Telethon Sistem višestrukih vektora i njegove primene
JP6698708B2 (ja) 2015-06-09 2020-05-27 ライフ テクノロジーズ コーポレーション 分子タグ付けのための方法、システム、組成物、キット、装置、及びコンピュータ可読媒体
WO2017024173A1 (en) 2015-08-04 2017-02-09 The Regents Of The University Of Colorado Multiplexed binary assembly and quantitative tracking of bacterial populations
WO2017075538A1 (en) 2015-10-29 2017-05-04 Amyris, Inc. Compositions and methods for production of myrcene
CN106811459B (zh) * 2015-11-27 2019-09-27 清华大学 一种利用短链引物阻遏提高dna克隆及组装效率的方法
JP6821598B2 (ja) 2015-12-07 2021-01-27 ザイマージェン インコーポレイテッド Ｃｏｒｙｎｅｂａｃｔｅｒｉｕｍ ｇｌｕｔａｍｉｃｕｍ由来のプロモーター
US11208649B2 (en) 2015-12-07 2021-12-28 Zymergen Inc. HTP genomic engineering platform
US9988624B2 (en) 2015-12-07 2018-06-05 Zymergen Inc. Microbial strain improvement by a HTP genomic engineering platform
EP4219731A3 (en) 2016-05-18 2023-08-09 Amyris, Inc. Compositions and methods for genomic integration of nucleic acids into exogenous landing pads
EP3478833A4 (en) 2016-06-30 2019-10-02 Zymergen, Inc. METHOD FOR PRODUCING A BACTERIAL HEMOGLOBIN LIBRARY AND ITS USE
JP2019519241A (ja) 2016-06-30 2019-07-11 ザイマージェン インコーポレイテッド グルコース透過酵素ライブラリーを生成するための方法およびその使用
GB201621589D0 (en) * 2016-12-19 2017-02-01 Univ Gent And Katholieke Univ Leuven K U Leuven R&D Polynucleotide shuffling method
EP3574105B1 (en) 2017-01-25 2021-04-21 Amyris Bio Products Portugal, Unipessoal, LDA Co-production of a sesquiterpene and a carotenoid
MX2019015505A (es) 2017-06-30 2020-07-28 Inscripta Inc Metodos, modulos, instrumentos y sistemas de procesamiento de celulas automatizados.
US10738327B2 (en) 2017-08-28 2020-08-11 Inscripta, Inc. Electroporation cuvettes for automation
CN111356766A (zh) 2017-09-18 2020-06-30 阿迈瑞斯公司 用于遗传工程改造克鲁维酵母宿主细胞的方法
US11685934B2 (en) 2017-09-18 2023-06-27 Amyris, Inc. Methods for genomic integration for Kluyveromyces host cells
WO2019055535A1 (en) 2017-09-18 2019-03-21 Amyris, Inc. METHODS OF GENOMIC INTEGRATION FOR HOST CELLS COMPRISING HANSENULA AND ARXULA
WO2019055481A1 (en) 2017-09-18 2019-03-21 Amyris, Inc. METHODS OF GENOMIC INTEGRATION IN PICHIA AND OTHER HOST CELLS
AU2018343309B2 (en) * 2017-09-29 2025-04-10 Victoria Link Limited Modular dna assembly system
EP3687621A4 (en) 2017-09-30 2021-08-11 Inscripta, Inc. Flow through electroporation instrumentation
WO2019190874A1 (en) 2018-03-29 2019-10-03 Inscripta, Inc. Automated control of cell growth rates for induction and transformation
US10376889B1 (en) 2018-04-13 2019-08-13 Inscripta, Inc. Automated cell processing instruments comprising reagent cartridges
WO2019201276A1 (en) * 2018-04-17 2019-10-24 Nanjingjinsirui Science & Technology Biology Corp. Dna construct design system
US10508273B2 (en) 2018-04-24 2019-12-17 Inscripta, Inc. Methods for identifying selective binding pairs
US10526598B2 (en) 2018-04-24 2020-01-07 Inscripta, Inc. Methods for identifying T-cell receptor antigens
US10858761B2 (en) 2018-04-24 2020-12-08 Inscripta, Inc. Nucleic acid-guided editing of exogenous polynucleotides in heterologous cells
AU2019292919A1 (en) 2018-06-30 2021-03-11 Inscripta, Inc. Instruments, modules, and methods for improved detection of edited sequences in live cells
US10532324B1 (en) 2018-08-14 2020-01-14 Inscripta, Inc. Instruments, modules, and methods for improved detection of edited sequences in live cells
US11142740B2 (en) 2018-08-14 2021-10-12 Inscripta, Inc. Detection of nuclease edited sequences in automated modules and instruments
US11965154B2 (en) 2018-08-30 2024-04-23 Inscripta, Inc. Detection of nuclease edited sequences in automated modules and instruments
EP3874037A4 (en) * 2018-10-31 2021-12-15 Zymergen, Inc. DETERMINIST MULTIPLEXED SET OF DNA LIBRARIES
WO2020203496A1 (ja) * 2019-04-01 2020-10-08 国立大学法人神戸大学 キメラプラスミドライブラリーの構築方法
US10920189B2 (en) 2019-06-21 2021-02-16 Inscripta, Inc. Genome-wide rationally-designed mutations leading to enhanced lysine production in E. coli
US10927385B2 (en) 2019-06-25 2021-02-23 Inscripta, Inc. Increased nucleic-acid guided cell editing in yeast
US12096784B2 (en) 2019-07-11 2024-09-24 Clara Foods Co. Protein compositions and consumable products thereof
BR112022000545A2 (pt) 2019-07-11 2022-04-05 Clara Foods Co Composições de proteína e produtos de consumo destas
US10927360B1 (en) 2019-08-07 2021-02-23 Clara Foods Co. Compositions comprising digestive enzymes
US10689669B1 (en) 2020-01-11 2020-06-23 Inscripta, Inc. Automated multi-module cell processing methods, instruments, and systems
JP7473953B2 (ja) * 2020-03-24 2024-04-24 国立大学法人 東京大学 連結ｄｎａの製造方法及びそれに用いるためのベクターの組み合わせ
WO2021211972A1 (en) * 2020-04-16 2021-10-21 Zymergen Inc. Circular-permuted nucleic acids for homology-directed editing
US20210332388A1 (en) 2020-04-24 2021-10-28 Inscripta, Inc. Compositions, methods, modules and instruments for automated nucleic acid-guided nuclease editing in mammalian cells
US11787841B2 (en) 2020-05-19 2023-10-17 Inscripta, Inc. Rationally-designed mutations to the thrA gene for enhanced lysine production in E. coli
CN112813103A (zh) * 2021-01-28 2021-05-18 云舟生物科技（广州）有限公司 一种标准品载体及其在测定重组腺相关病毒滴度中的应用
CN113943666B (zh) * 2021-11-11 2023-08-22 广西壮族自治区农业科学院 一种酿酒酵母工程菌及其应用
CN115261377B (zh) * 2021-12-27 2024-11-19 天津大学 一种dna串联重复单元的拆分组装方法

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030022179A1 (en) * 2000-08-21 2003-01-30 Chesnut Jonathan D. Methods and reagents for molecular cloning
CN1427079A (zh) * 2002-09-30 2003-07-02 华南农业大学 多基因载体的构建方法与应用
WO2007038276A2 (en) * 2005-09-22 2007-04-05 Intrexon Corporation Dna modular cloning vector plasmids and methods for their use
WO2008045380A2 (en) * 2006-10-04 2008-04-17 Codon Devices, Inc. Nucleic acid libraries and their design and assembly

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5057419A (en) * 1988-09-22 1991-10-15 Rutgers University Genetically engineered plasmid and organisms for the production of specialized oils
US5976846A (en) * 1996-01-13 1999-11-02 Passmore; Steven E. Method for multifragment in vivo cloning and mutation mapping
WO1999016904A1 (en) * 1997-09-29 1999-04-08 City Of Hope Efficient linking of nucleic acid segments
ATE423845T1 (de) * 1999-03-18 2009-03-15 Complete Genomics As Methoden zur klonierung und herstelung von kettenfragmenten mit lesbarem inhalt an information
CA2317865A1 (en) 2000-03-07 2001-09-07 University Of Guelph Dna joining method
US20030087254A1 (en) * 2001-04-05 2003-05-08 Simon Delagrave Methods for the preparation of polynucleotide libraries and identification of library members having desired characteristics
US20020165175A1 (en) * 2001-04-17 2002-11-07 Xiaowu Liang Fast and enzymeless cloning of nucleic acid fragments
CA2618699C (en) * 2005-08-11 2012-10-02 J. Craig Venter Institute, Inc. In vitro recombination method
CA2618665C (en) * 2005-08-11 2012-11-13 J. Craig Venter Institute Method for in vitro recombination
US20100291633A1 (en) 2007-09-03 2010-11-18 Thorsten Selmer Method of cloning at least one nucleic acid molecule of interest using type iis restriction endonucleases, and corresponding cloning vectors, kits and system using type iis restriction endonucleases
CN101932725A (zh) * 2007-10-08 2010-12-29 合成基因组股份有限公司 大核酸的装配
WO2010059763A1 (en) * 2008-11-19 2010-05-27 Amyris Biotechnologies, Inc. Compositions and methods for the assembly of polynucleotides

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030022179A1 (en) * 2000-08-21 2003-01-30 Chesnut Jonathan D. Methods and reagents for molecular cloning
CN1427079A (zh) * 2002-09-30 2003-07-02 华南农业大学 多基因载体的构建方法与应用
WO2007038276A2 (en) * 2005-09-22 2007-04-05 Intrexon Corporation Dna modular cloning vector plasmids and methods for their use
WO2008045380A2 (en) * 2006-10-04 2008-04-17 Codon Devices, Inc. Nucleic acid libraries and their design and assembly

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US8110360B2 (en) 2012-02-07
US20100136633A1 (en) 2010-06-03
SG171760A1 (en) 2011-07-28
CA2744153A1 (en) 2010-05-27
ZA201103637B (en) 2012-08-29
JP2012509084A (ja) 2012-04-19
US8221982B2 (en) 2012-07-17
CA2744153C (en) 2020-07-07
KR20110102351A (ko) 2011-09-16
US8546136B2 (en) 2013-10-01
AU2009316660A1 (en) 2011-07-07
BRPI0922187A2 (pt) 2018-10-23
BRPI0922187B1 (pt) 2021-05-04
EP2358875B1 (en) 2015-08-12
US20120245056A1 (en) 2012-09-27
AU2009316660B2 (en) 2015-01-29
MX2011005195A (es) 2011-09-01
US20100124768A1 (en) 2010-05-20
WO2010059763A1 (en) 2010-05-27
KR101794298B1 (ko) 2017-11-20
EP2358875A1 (en) 2011-08-24
CN102282255A (zh) 2011-12-14

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