JP2012240960A - Deodorant - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a deodorant capable of showing superior deodorant effects by decomposing causative substances of body odors (in particular, aging odors).SOLUTION: Alcohol dehydrogenase derived from yeast and Pyrobaculum archaebacteria has effects on decomposition of nonenal, which is a causative substance of the aging odors, for deodorization, and is effective as a deodorant removing body odors.

Description

  The present invention relates to a deodorant, and more particularly, to a deodorant preferably used for removing body odor.

  In recent years, awareness of etiquette has spread to businessmen and middle-aged and elderly people, and much attention has been paid to body odor prevention. In particular, the aging odor has become a serious problem for middle-aged and older people who want to be refreshing and youthful forever.

  Age-related odor is known to be the main causative component, such as nonenal produced by oxidation of lower fatty acids that increase in sebum, and increases with aging, especially in middle-aged and older people after the age of 40 Easy body odor.

  For removal of aging odor, (i) masking the causative substance with a fragrance, (ii) preventing oxidation of lower fatty acids in sebum with an antioxidant, and suppressing the generation of the causative substance, (iii) antibacterial agent It is considered indispensable to use any of the following mechanisms: suppressing the growth of resident skin bacteria, (iv) capturing and eliminating the odor of the causative substance, and (v) decomposing the causative substance. Examples of deodorant components that can remove aging odors so far include ethanolamine (Patent Document 1), sake lees extract containing rice fermentation liquor (Patent Document 2), brown algae extract (Patent Document 2), and the like. Yes.

  However, the conventionally reported deodorant components have a problem that they are not satisfactory in terms of effectiveness, safety and usability. In particular, most of the deodorant components reported so far utilize the mechanisms (i) to (iv) described above, and the aging odor is effectively eliminated by decomposing the causative substances of the aging odor. However, there are few reports on what can be removed.

  Due to the current state of the prior art and the recent increase in etiquette awareness, the development of a new deodorant that has never existed in the past, especially a deodorant that can be deodorized by decomposing the causative substance of an aging odor, is eagerly desired. Yes.

JP 2001-97838 A JP 2008-184395 A

  An object of this invention is to provide the deodorizer which can exhibit the outstanding deodorizing effect | action by decomposing | disassembling the causative substance of body odor (especially age-related odor).

  As a result of diligent studies to solve the above-mentioned problems, the present inventors have found that alcohol dehydrogenase derived from yeast and Pseudobacurum archaea has a deodorizing action by degrading nonenal, which is a causative substance of aging odor. And has been found to be effective as a deodorant for removing body odor. The present invention has been completed by further studies based on such knowledge.

That is, this invention provides the deodorant and cosmetics which are hung up below.
Item 1. A deodorizer characterized by comprising an alcohol dehydrogenase derived from yeast or Pseudobacum archaebacterium.
Item 2. Item 2. The deodorizer according to Item 1, further comprising at least one reduced coenzyme selected from the group consisting of NADH and NADPH.
Item 3. Item 3. The deodorant according to Item 1 or 2, wherein the alcohol dehydrogenase is derived from Saccharomyces cerevisiae.
Item 4. Item 4. The deodorizer according to any one of Items 1 to 3, comprising a yeast extract as the alcohol dehydrogenase.
Item 5. Item 5. The deodorant according to any one of Items 1 to 4, which is for body odor prevention.
Item 6. Item 6. The deodorant according to Item 5, wherein the body odor is an aging odor.
Item 7. Cosmetics containing the deodorizer in any one of claim | item 1 -6.
Item 8. Item 8. A cosmetic according to Item 7, which is a cosmetic for preventing body odor.
Item 9. Item 9. The cosmetic according to Item 8, wherein the body odor is an aging odor.

  The deodorant of the present invention can decompose body odor-causing substances such as an aging odor, has an excellent body odor prevention effect, and can bring the gospel to those who suffer from body odor.

The result of searching for an enzyme capable of degrading nonenal in Test Example 1 is shown. In addition, the unit (unit / mg) of the vertical axis | shaft in FIG. 1 shows nonenal decomposition | disassembly activity (U) per mg of enzyme. In Test example 2, the result of having evaluated the nonenal degradability by a yeast extract is shown. In addition, the unit (unit / ml) of the vertical axis | shaft in FIG. 2 shows nonenal decomposition | disassembly activity (U) per 1 ml of each reaction solution.

1. Deodorant The deodorizer of the present invention is characterized by containing an alcohol dehydrogenase derived from yeast or Pseudobacum archaebacterium. Hereinafter, the present invention will be described in detail.

  As the alcohol dehydrogenase used in the present invention, those derived from yeasts or archaea of the genus Purobacurum are used. By selecting and using a specific alcohol dehydrogenase in this way, it is possible to deodorize body odors, especially deodorizing aging odors. The deodorant mechanism of the deodorant of the present invention is not intended to be limited, but the alcohol dehydrogenase derived from yeast or purobacurum archaea is 2-nonenal, which is a causative substance of body odor, as an odorless substance. It is considered that a deodorizing effect is exhibited by reductive decomposition to certain 2-nonen-1-ol.

  In the present invention, the genus of yeast that is a microorganism derived from alcohol dehydrogenase is not particularly limited, and examples include Saccharomyces, Pichia, Schizosaccharomyces, and the like. Examples include Saccharomyces cerevisiae, Pichia pastoris, and Schizosaccharomyces pombe. Among these, yeasts belonging to the genus Saccharomyces, particularly Saccharomyces cerevisiae, possess alcohol dehydrogenase with particularly excellent body odor removing ability, and are suitable as the yeast derived from the alcohol dehydrogenase used in the present invention.

  In the present invention, the species of Pyrobaculum archaea, which is a microorganism from which alcohol dehydrogenase is derived, is not particularly limited. Pyrobaculum calidihontis, Pyrobaculum arsenaticum, etc. are mentioned. Among these, purobacrum aerophilum possesses alcohol dehydrogenase with particularly excellent body odor removing ability and is suitable as an archaebacteria derived from the alcohol dehydrogenase used in the present invention.

  Preferable examples of the alcohol dehydrogenase used in the present invention include alcohol dehydrogenase whose enzyme number corresponds to EC.1.1.1.1 or EC.1.1.1.2.

  The alcohol dehydrogenase used in the present invention is preferably derived from yeast, and more preferably derived from Saccharomyces cerevisiae, from the viewpoint of achieving a further excellent body odor prevention effect.

  In the present invention, the alcohol dehydrogenase may not be purified. Specifically, a crude product of the alcohol dehydrogenase may be used as the alcohol dehydrogenase, and yeast or purobacurum archaea as long as the alcohol dehydrogenase is contained in a state of retaining activity. An extract of

  In addition, the alcohol dehydrogenase used in the present invention may be one isolated from yeast or archaea of the genus Purobacurum, its mutant, one produced by gene recombination technology, and the like.

  In the present invention, the alcohol dehydrogenase may be selected from one of yeast-derived and Purobacurum archaebacteria and used alone or in combination of two or more.

  The deodorizer of the present invention, together with the alcohol dehydrogenase, contains at least one reduced complement selected from the group consisting of NADH (reduced nicotinamide adenine dinucleotide) and NADPH (reduced nicotinamide adenine dinucleotide phosphate). It is desirable to contain an enzyme. By using these reduced coenzymes, effective body odor (especially aging odor) can be eliminated. In particular, it is desirable to use NADH as a reduced coenzyme when using an alcohol dehydrogenase derived from yeast, and NADPH as a reduced coenzyme when using an alcohol dehydrogenase derived from Pseudobacterium archaebacterium. Is desirable.

  The ratio of the alcohol dehydrogenase and the reduced coenzyme is not particularly limited. For example, the reduced coenzyme is usually 0.1 nmol to 10 μmol, preferably 0.5 nmol to 1 μmol with respect to 1 U of the alcohol dehydrogenase. More preferably, the ratio is 1 nmol to 100 nmol. In the present specification, the unit of alcohol dehydrogenase activity (hereinafter also referred to as nonenal degradation activity) is 1 mM trans-2-nonenal and 0.3 mM in 50 mM sodium acetate buffer (pH 6.0). The amount of enzyme required to oxidize 1 μmol of NADH or NADPH per minute at 37 ° C. in the presence of NADH or NADPH and 20 wt% DMSO (dimethyl sulfoxide) is defined as 1 U.

  As the alcohol dehydrogenase, when using an extract of yeast or purobacurum archaea, the reduced coenzyme is contained in these extracts, so the reduced coenzyme is not added separately. Alternatively, it may be sufficient to supplement the reduced coenzyme with an appropriate amount.

  The deodorant of the present invention is suitable for use for body odor prevention, particularly for aging odor prevention, and is used by being applied percutaneously to a skin site emitting body odor. That is, the deodorant of the present invention is preferably used as an additive blended in cosmetics applied to the skin and scalp. Below, the cosmetics with which the deodorant of this invention was mix | blended are demonstrated.

2. Cosmetics The cosmetics of the present invention are characterized by containing the above deodorant.

  In the cosmetic of the present invention, the content ratio of the deodorant is appropriately set according to the formulation form and the like. For example, the alcohol dehydrogenase is 0.01 to 100 U /% of the total amount of the cosmetic. ml, preferably 0.05 to 50 U / ml, more preferably 0.1 to 10 U / ml.

  The cosmetic of the present invention is prepared in various forms by combining a pharmaceutically or cosmetically acceptable base or carrier in addition to the above deodorant. As the pharmaceutically or cosmetically acceptable base and carrier, known ones used in cosmetics can be used.

  Moreover, various additives mixed with cosmetics can be blended with the cosmetics of the present invention as needed. Examples of such additives include humectants, whitening agents, anti-inflammatory agents, cooling agents, UV absorbers, UV scattering agents, vitamins, plant extracts, skin astringents, cell activators, vasodilators, blood circulation Accelerators, skin function enhancers, bactericides (antibacterial agents), pH adjusters, thickeners, antioxidants, sequestering agents, surfactants, emulsifiers, dyes (dyes, pigments), fragrances, preservatives, etc. Can be mentioned.

  The cosmetic of the present invention preferably contains water in order to effectively exhibit the activity of the alcohol dehydrogenase when applied to the skin or scalp. The content of water contained in the cosmetic of the present invention is not particularly limited. For example, it is usually 1 to 99% by weight, preferably 5 to 95% by weight, more preferably 10 to 10% by weight based on the total amount of the cosmetic. 90% by weight.

  The form of the cosmetic preparation of the present invention is not particularly limited as long as it can be applied transdermally. For example, it is paste, mousse, gel, liquid, emulsion, suspension, cream, Examples include ointments, aerosols, sprays, liniments and the like. Moreover, when the cosmetic preparation of the present invention is in the form of liquid, emulsion, or suspension, it may be in a state of impregnating a sheet substrate such as a nonwoven fabric.

  The cosmetic of the present invention is suitable for preventing body odor, particularly for preventing aging odor, and is applied to the skin site or scalp where the body odor is worrisome.

About the quantity and frequency | count which apply the cosmetics of this invention to skin, although it sets suitably according to the content rate of the said alcohol dehydrogenase, the intensity | strength of body odor, etc., for example once a day or 2-5 times What is necessary is just to apply to skin the quantity which is converted into the quantity of the said alcohol dehydrogenase per cm < ² > of skin, and is 0.001-0.1U, Preferably about 0.01-0.05U.

  EXAMPLES Hereinafter, although a test example is given and this invention is demonstrated, this invention is not limited to these Examples.

Test Example 1 : Search for Nonenal Degrading Enzyme 1) Method 50 mM sodium acetate containing 1 mM trans-2-nonenal, 50 μg / ml alcohol dehydrogenase, 0.3 mM NADH or NADPH, and 20 wt% DMSO (dimethyl sulfoxide) 1 ml of buffer solution (pH 6.0) was prepared, and absorbance at 340 nm was measured while incubating at 37 ° C. for 3 minutes. Nonenal degradation activity was calculated from the rate of decrease in absorbance per minute. The mmol molecular absorption coefficient of NADH or NADPH was 6.22 mM ⁻¹ cm ⁻¹ .
In this test, eight types of microorganism-derived substances shown in Table 1 were used as alcohol dehydrogenase.

2) Results Trans-2-nonenal is a causative substance of an aging odor, and when it is reduced and decomposed by alcohol dehydrogenase, odorless substance trans-2-nonen-1-ol is produced. During this reductive decomposition, NADH or NADPH is converted to NAD ⁺ or NADP ⁺ . In this test, the degree of degradation of trans-2-nonenal was evaluated by measuring the decrease in NADH or NADPH.

  The obtained results are shown in FIG. FIG. 1A shows the result of using NADH as a reduced coenzyme, and FIG. 1B shows the result of using NADPH as a reduced coenzyme.

  As is apparent from FIG. 1, among alcohol dehydrogenases, when Saccharomyces cerevisiae and Purovacrum aerophilumum-derived ones were used, it was confirmed that nonenal was decomposed, and when other alcohol-derived alcohol dehydrogenases were used. In this case, nonenal was not decomposed. That is, it has been clarified that, by selecting and using alcoholic dehydrogenases derived from Saccharomyces cerevisiae and Pyrovacrum aerophilum, the causative agent of aging odor can be decomposed and deodorized for the first time.

  Further, from this result, it is desirable to use NADH as a reduced coenzyme when using an alcohol dehydrogenase derived from Saccharomyces cerevisiae, and as a reduced coenzyme when using an alcohol dehydrogenase derived from Purovacrum aerophilum. It has also been confirmed that it is desirable to use NADPH.

Test Example 2 : Evaluation of Nonenal Degradability by Yeast Extract 1) Method
Preparation of yeast extract A liquid containing Saccharomyces cerevisiae NBRC2018, 0.2 wt% glucose, 0.5 wt% peptone (Nippon Pharmaceutical), 0.2 wt% dry yeast extract (Nacalai Tesque) The culture was allowed to stand for 2 days at 30 ° C. in 10 ml of a medium (pH 7.0). After incubation, the mixture was centrifuged at 6000 rpm and 4 ° C. for 10 minutes to precipitate the yeast. The supernatant was discarded, and 1 ml of 20 mM potassium phosphate buffer (pH 7.0) containing 100 mM sodium chloride was added to the precipitate for suspension, and the mixture was again centrifuged at 6000 rpm at 4 ° C. for 10 minutes to precipitate the yeast. To the precipitate, 0.9 ml of 20 mM potassium phosphate buffer (pH 7.0) containing 100 mM sodium chloride was added and suspended, and 0.1 ml of 0.5 mg / ml Lyticase was added. This solution was incubated at 30 ° C. for 1 hour to lyse the yeast cell walls. Subsequently, the cells were disrupted by ultrasonic disruption, and the resulting disrupted solution was centrifuged at 12,000 rpm, 4 ° C., 10 minutes, and the supernatant was recovered. This crushed supernatant was used as a yeast extract for subsequent operations.

It was 0.1 U / ml when it measured about the alcohol dehydrogenase activity (nonenal decomposition | disassembly activity) of the obtained yeast extract. Alcohol dehydrogenase activity (nonenal decomposition activity) is 50 mM sodium acetate buffer containing 1 mM trans-2-nonenal, 10 wt% yeast extract, 0.3 mM NADH, and 20 wt% DMSO (dimethyl sulfoxide). 1 ml of a solution (pH 6.0) was prepared, the absorbance at 340 nm was measured while incubating at 37 ° C. for 3 minutes, and the amount of enzyme required to oxidize 1 μmol of NADH per minute was calculated as 1 U. The NADH molecular extinction coefficient was 6.22 mM ⁻¹ cm ⁻¹ .

Decomposition reaction of nonenal 0.9 ml of 50 mM sodium acetate buffer (pH 6.0) containing 1 mM trans-2-nonenal, 0.3 mM NADH, and 20 wt% DMSO (dimethyl sulfoxide) was prepared and reacted. It was set as the solution. After the reaction solution was prepared, it was preincubated at 37 ° C. for 1 minute. Next, 0.1 ml of yeast extract was added, and the absorbance at 340 nm was measured while incubating at 37 ° C. for 3 minutes (Sample 9). Nonenal degradation activity was calculated from the rate of decrease in absorbance per minute. The mmol molecular absorption coefficient of NADH or NADPH was 6.22 mM ⁻¹ cm ⁻¹ . Moreover, as a blank, when using ultrapure water instead of trans-2-nonenal (blank 1), a 20 mM potassium phosphate buffer (pH 7.0) containing 100 mM sodium chloride is used instead of the yeast extract. When used (blank 2), the same measurement was performed.

2) Results The results obtained are shown in FIG. From this result, when no trans-2-nonenal or yeast extract was added (blanks 1 and 2), a decrease in NADH (decrease in absorbance at 340 nm) was not observed, but the yeast extract and trans- In the sample to which 2-nonenal was added, NADH was significantly reduced, and trans-2-nonenal was decomposed.

  From the above results, it was shown that nonenal can be decomposed even when yeast extract is used. The yeast extract used in this test contains alcohol dehydrogenase in a state of maintaining activity, so it is considered that nonenal was deodorized.

Test Example 3 : Confirmation test of deodorizing effect 1) Method 1 ml of four kinds of odor samples shown in Table 2 were put in a tube, covered and sealed. In this state, it was incubated at 37 ° C. for 5 minutes. Then, the lid was opened and the odor of the odor sample was evaluated. Odor was evaluated by scoring according to the following criteria by six panelists.

<Criteria for odor>
Score 0: No aging odor (trans-2-nonenal odor) is felt.
1: Almost no aging odor (trans-2-nonenal odor).
2: Slightly feels aging odor (trans-2-nonenal odor).
3: Feels aging odor (trans-2-nonenal odor).
4: Aging odor (trans-2-nonenal odor) is strongly felt.

2) Results Table 3 shows the results obtained. From this result, in the odor samples (odor samples 2 and 4) containing alcohol dehydrogenase derived from Saccharomyces cerevisiae and Purovacrum aerophilum, the aging odor caused by trans-2-nonenal was clearly suppressed. Also from this result, it was confirmed that the aging odor can be deodorized by using alcohol dehydrogenase derived from Saccharomyces cerevisiae and Purovacrum aerophilum.

Production Examples The following are formulation examples of various cosmetic preparations using the deodorant of the present invention.
Production Example 1: Deodorizing skin cleanser yeast-derived alcohol dehydrogenase 5 U / ml
NADH 250 nmol / ml
Benzalkonium chloride 0.05% by weight
L-menthol 0.01% by weight
Polyoxyethylene (20) lauryl ether 1% by weight
pH adjuster appropriate amount perfume appropriate amount preservative appropriate amount
Purified water balance
Total 100% by weight

Production Example 2: Deodorizing lotion yeast-derived alcohol dehydrogenase 3 U / ml
NADH 150 nmol / ml
Glycerin 3% by weight
Ethanol 1.5% by weight
Potassium sorbate 0.2% by weight
Purified water balance
Total 100% by weight

Production Example 3: Deodorizing Sheet A liquid tissue having the following composition is impregnated into a cellulose / rayon blended nonwoven fabric to obtain a wet tissue type deodorizing sheet.
Composition of solution Yeast-derived alcohol dehydrogenase 6U / ml
NADH 300 nmol / ml
Benzalkonium chloride 0.05% by weight
Polyglyceryl monolaurate 0.4% by weight
1% ethanol
Propylene glycol 0.5% by weight
L-menthol 0.01% by weight
pH adjuster appropriate amount perfume appropriate amount preservative appropriate amount
Purified water balance
Total 100% by weight

Claims (9)

  A deodorizer characterized by comprising an alcohol dehydrogenase derived from yeast or Pseudobacum archaebacterium.   The deodorizer according to claim 1, further comprising at least one reduced coenzyme selected from the group consisting of NADH and NADPH.   The deodorizer according to claim 1 or 2, wherein the alcohol dehydrogenase is derived from Saccharomyces cerevisiae.   The deodorant in any one of Claims 1-3 which contains a yeast extract as alcohol dehydrogenase.   The deodorizer according to any one of claims 1 to 4, which is for body odor prevention.   The deodorizer according to claim 5, wherein the body odor is an aging odor.   Cosmetics containing the deodorizer in any one of Claims 1-6.   The cosmetic according to claim 7, which is a cosmetic for preventing body odor. The cosmetic according to claim 8, wherein the body odor is an aging odor.

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