WO2020013310A1 - Method for inhibiting odor formation - Google Patents

Abstract

A method for inhibiting odor formation that comprises contacting component (a) and water with a microorganism and thus reducing the amount of an odor substance produced by the microorganism, wherein, at the pH value of a composition comprising component (a) and water, the logD value of component (a) calculated in accordance with formula (1) is -1.5 or more and not more than 1.5. Component (a): an organic carboxylic acid or a salt thereof. logD=ClogP-log[1+10^(pH-pKa)] formula (1) [In formula (1): ClogP represents the calculated logP value of the logarithmic value of the water/octanol distribution coefficient of component (a) in the acid state; pH represents the pH value of water containing component (a); and pKa represents the pKa value of component (a)].

Description

How to control odor generation

The present invention relates to a method for suppressing odor generation.
The invention further relates to a method for controlling pH, for example in the fields of biology, biochemistry and physiological sciences.

In recent years, with the growing interest in the living environment of consumers, it has been desired to reduce or eliminate unpleasant odors around them. Textile products that come into direct contact with human skin, such as underwear, towels, and handkerchiefs, or textile products that may absorb or adhere to sweat or keratin containing sebum may produce a unique odor. is there.

Until now, methods for suppressing the generation of odor by controlling the metabolic reaction to the odor-causing substance that occurs in the cells of the causative bacteria (microorganisms) that produce the odor-causing substance have been studied.
For example, Patent Literature 1 discloses a ketone compound having a specific structure as an agent for suppressing the conversion of sebum dirt components to 4-methyl-3-hexenoic acid, which is one of the substances causing a dry odor. . Patent Document 2 discloses a macrocyclic ketone compound as an agent that inhibits the activity of β-gluconidase and suppresses the generation of urine odor. There is also known a technique in which an organic carboxylic acid having a specific structure, such as benzoic acid, is used to sterilize microorganisms to suppress generation of odor.

On the other hand, it is known that the activity of fatty acid metabolism-related enzymes is suppressed by setting the pH environment in the cytoplasm of the bacteria causing the odor to an acidic side (see Non-Patent Document 1), and the intracellular pH is controlled. A way to do that is being considered.
For example, Patent Document 3 discloses that the pH in a vacuole of a plant cell is adjusted using a nucleic acid molecule encoding a polypeptide having an activity of changing the pH in a cell such as a plant cell. . Patent Literature 4 discloses a pH probe capable of measuring intracellular pH, and indicates a need to control intracellular pH. Patent Literature 5 describes administering an effective amount of a drug that increases intracellular pH.

However, it has not been known to control odor generation by controlling the pH environment in the cytoplasm of bacteria causing odor.

JP 2012-127012 A International Publication No. 2009/037861 International Publication No. 2007/137345 International Publication No. WO 2016/138116 International Publication No. WO 2016/142445

The present invention relates to a method for suppressing the generation of odor by contacting the following component (a), water and a microorganism to suppress the amount of odorous substances produced by the microorganism, wherein the pH of the composition comprising the component (a) and water is adjusted. Wherein the logD of the component (a) calculated by the following formula (1) is -1.5 or more and 1.5 or less.

Component (a): organic carboxylic acid or salt thereof
logD = ClogP-log [1 + 10 ^(pH-pKa) ] Formula (1)
[In the formula (1), ClogP is a calculated logP value of a logarithmic value of a water / octanol partition coefficient in the acid form of the component (a), pH is the pH of water containing the component (a), and pKa Is the pKa of the component (a). ]

Further, the present invention provides that, in two phases containing water separated by a surfactant film, the logD of the component (a) present in one of the phases calculated by the formula (1) is -1.5 or more and 1 or more. The present invention relates to a method for controlling pH, wherein the pH of the other phase separated by the surfactant film is lowered by controlling the pH to 0.5 or less.
The above and other features and advantages of the present invention will become more apparent from the following description.

DETAILED DESCRIPTION OF THE INVENTION

臭 Many of the odor generation inhibitors disclosed in Patent Literature 1 or Patent Literature 2 have an odor itself, and thus need to be used in an appropriate usage scene and amount. In addition, from the viewpoint of coexistence with the environment, it may be necessary to examine the suppression of odor generation without particularly sterilizing microorganisms.

Therefore, the present invention relates to an odor generation suppressing method capable of suppressing odor generation from odor-causing bacteria without sterilizing microorganisms that cause odor.
The invention further relates to a method for controlling the pH of one of the two phases containing water separated by a surfactant membrane.

The present inventor has conducted intensive studies from the viewpoint of the odor-causing substance, the causative bacteria, and the mechanism of generation. As a result, the organic acid or its salt and the microorganism can be combined with each other in water when the logD of the organic acid or its salt having a carboxyl group in water is in the range of -1.5 or more and 1.5 or less in water. It has been found that, by contact, the generation of the odor generated by the microorganism can be suppressed while maintaining the number of microorganisms, and the pH of the microorganism in the cell membrane can be reduced.
The present invention has been completed based on these findings.

ADVANTAGE OF THE INVENTION According to the odor generation suppression method of this invention, the odor generation from the odor-causing bacteria can be suppressed, without sterilizing the odor-causing microorganisms.
Further, the present invention can efficiently control the pH of one of the two phases containing water separated by a surfactant membrane.

<(A) component>
The component (a) used in the method of the present invention is an organic carboxylic acid or a salt thereof.
The component (a) is brought into contact with a microorganism in water in a state where the logD of the component (a) at the pH of the water containing the component (a) is −1.5 or more and 1.5 or less, so that the microorganism is contained in the cell membrane of the microorganism. PH decreases. As a result, the component (a) easily penetrates into the microorganism, and the component (a) penetrated into the microorganism suppresses the function of the metabolic system that produces odorous substances in the microorganisms, thereby suppressing the production of odorous substances. Can be. In particular, by infiltrating the component (a) into the microorganism, the intracellular pH is reduced, and as a result, the activity of fatty acid metabolism-related enzymes can be suppressed, and the metabolism of fatty acids can be controlled.
Further, the component (a) is converted into one phase separated by a surfactant film in a state where the logD of the component (a) at the pH of water containing the component (a) is −1.5 or more and 1.5 or less. By causing the component (a) to be present, the component (a) is adsorbed on the surfactant film, and is easily distributed to the other phase where the component (a) is not present, separated by the surfactant film. As a result, it is possible to control the pH of the other phase separated by the surfactant and to which the component (a) is distributed. As used herein, "controlling the pH" includes lowering the pH.

In the present invention, the logD of the component (a) calculated from the following formula (1) at the pH of the composition containing the component (a) and water is from −1.5 to 1.5.

logD = ClogP-log [1 + 10 ^(pH-pKa) ] Formula (1)

[In the formula (1), ClogP is a calculated logP value of a logarithmic value of a water / octanol partition coefficient in the acid form of the component (a), pH is the pH of water containing the component (a), and pKa Is the pKa of the component (a). ]

In the present invention, logD can be calculated based on the ClogP of the acid form of the component (a), the acid dissociation constant (pKa), and the pH of the composition containing the component (a) and water. logD is a calculated value of the water / octanol partition coefficient in consideration of the acid-form ClogP of the component (a), the pH of water containing the component (a), and pKa.
The concept of logD is described in, for example, Li Xing, Robert C. Glen, Novel Methods for the Prediction of logP, pKa, and logD, J. Chem. Inf. Comput. Sci. 2002, vol. 42, p. 796-805. It is a disclosed and well-known concept.
In the present invention, in order to calculate logD, the value of ClogP and the value of pKa are calculated by thermodynamic property estimation software “COSMOtherm” (Morcis).

Specific examples of the component (a) used in the present invention include an aliphatic polycarboxylic acid having 8 to 14 carbon atoms or a salt thereof. The component (a) preferably has 8 or more carbon atoms, more preferably 9 or more carbon atoms, and preferably 14 or less, and more preferably 12 or less, in that the logD can be easily adjusted to a desired range. And more preferably 10 or less.
As the component (a) used in the present invention, the ratio of the molecular weight of the component (a) to the molecular weight per carboxyl group, (molecular weight of the component (a)) / The value represented by (molecular weight of carboxyl group) is preferably 1.9 or more, more preferably 2.0 or more, and even more preferably 2.2 or more. From the same viewpoint, the value represented by (molecular weight of component (a)) / (molecular weight of carboxyl group) is preferably 3.5 or less, more preferably 3.2 or less, more preferably 3.0 or less, and 2 .8 or less is more preferable. By setting the value represented by (molecular weight of component (a)) / (molecular weight of carboxyl group) in the above numerical range, the logD is preferably controlled within a desired range without sterilizing microorganisms. In the present invention, the molecular weight of the carboxyl group is 45, which is the value of the molecular weight of the acid type carboxyl group, that is, COOH. The carboxylic acid used for calculating the molecular weight of the component (a) uses an acid type value.
As the component (a) used in the present invention, one or more compounds selected from the group consisting of aliphatic dicarboxylic acids and salts thereof are preferable, and specific examples thereof include suberic acid (8 carbon atoms) and azelaic acid. (C9), sebacic acid (C10), dodecanedioic acid (C12), tetradecandioic acid (C14), and one or more compounds selected from the group consisting of salts thereof. Is mentioned. Further, examples of the salt include an alkali metal salt such as a sodium salt and an alkaline earth metal salt such as a magnesium salt.

Another specific example of the component (a) used in the present invention is an organic carboxylic acid having one naphthalene group and one carboxyl group from the viewpoint of easily controlling logD calculated by the above formula (1) to a desired range. Acids or salts thereof. Such an organic carboxylic acid may have an arbitrary hydroxyl group. Further, examples of the salt include an alkali metal salt such as a sodium salt and an alkaline earth metal salt such as a magnesium salt.
Specific examples include 1-naphthalenecarboxylic acid, 2-naphthalenecarboxylic acid, 2-hydroxy-1-naphthalenecarboxylic acid, 3-hydroxy-2-naphthalenecarboxylic acid, 6-hydroxy-2-naphthalenecarboxylic acid, and One or more compounds selected from the group consisting of these salts are preferred.

市 販 In the present invention, a commercially available component (a) may be used. Alternatively, the component (a) can be synthesized by an ordinary production method.

The content of the component (a) with respect to the mass of the composition containing the component (a) and water is preferably 0.01% by mass or more, more preferably 0.03% by mass or more, and still more preferably 0% by mass or more. 0.05% by mass or more, more preferably 0.08% by mass or more. The content of the component (a) is preferably 1% by mass or less, more preferably 0.8% by mass or less, and further preferably 0.5% by mass or less.

<Water>
As the water used in the method of the present invention, ion-exchanged water, distilled water, tap water, sweat, rain and the like can be used.

The composition containing component (a) and water may contain optional components such as surfactants and fragrances as long as the effects of the present invention are not impaired.
For example, in a composition containing the component (a) and water, as a component (b), a surfactant, preferably a (b1) nonionic surfactant and a (b2) anion, as long as the effects of the present invention are not impaired. One or more surfactants selected from the group consisting of surfactants (however, excluding the component (a)) can be contained.

As the component (b1), a nonionic surfactant having an HLB of 9 or more and 15 or less is preferable from the viewpoint that the effect of suppressing the generation of odor by the component (a) can be further improved. In the case of a nonionic surfactant having a polyoxyethylene group, the HLB of the component (b1) is determined by a Griffin method represented by the following formula (2).

HLB value = [(molecular weight of polyoxyethylene group portion of component (b1)) / (molecular weight of component (b)]] × 20 (2)

The molecular weight of the polyoxyethylene group portion of the component (b1) is calculated using the value of the average number of moles of polyoxyethylene group added.
In the case of a nonionic surfactant having no polyoxyethylene group, it can be determined by the Davis method. Here, the method of calculating the HLB by the Davis method is described in pages 24 to 25 of “Surfactant Physical Properties / Application / Chemical Ecology” (published by Kodansha Scientific, May 20, 1990, 7th print, Fumio Kitahara et al.). Can be calculated by using the method described in (1).

The HLB of the component (b1) is preferably 9.0 or more, more preferably 9.5 or more, and more preferably 9.5 or more, from the viewpoint of excellent cleaning of dirt attached to textiles and the like (hereinafter, also referred to as cleaning performance). Is at least 10, more preferably at least 10.5, even more preferably at least 11.0. The HLB is preferably 15 or less, more preferably 14.5 or less, more preferably 14 or less, still more preferably 13 or less, and still more preferably 12 or less.

Specific examples of the component (b2) include one or more anionic surfactants selected from the group consisting of the following components (b21), (b22), (b23) and (b24).

(B21) Component: alkyl or alkenyl sulfate ester salt (b22) Component: polyoxyalkylene alkyl ether sulfate salt or polyoxyalkylene alkenyl ether sulfate salt (b23) component: anionic surfactant having a sulfonate group (b24) Ingredients: fatty acids having 10 to 20 carbon atoms or salts thereof (however, excluding component (b23))

The content of the component (b) with respect to the mass of the composition is preferably 0.1% by mass or more, more preferably 0.3% by mass or more, and still more preferably 0.4% by mass, from the viewpoint of further enhancing the odor generation suppressing effect. %, More preferably 0.5% by mass or more. From the same viewpoint, the content of the component (b) is preferably 10% by mass or less, more preferably 9% by mass or less, still more preferably 8% by mass or less, and still more preferably 5% by mass or less.

<Surfactant film>
In the present specification, “surfactant film” means a film formed by a surfactant.
Examples of the surfactant film to which the present invention can be applied include a film formed of a surfactant having an aliphatic hydrocarbon group having 10 to 22 carbon atoms. The surfactant film may be a monomolecular film formed by arranging surfactant molecules in a single layer, or the hydrophobic portion of the surfactant molecules arranged without gaps on the inside and the hydrophilic portion on the outside. It may be a double film formed to face. As a specific example of the surfactant film, a film formed of a cationic surfactant having an aliphatic hydrocarbon group having 10 to 22 carbon atoms, having an aliphatic hydrocarbon group having 10 to 22 carbon atoms A film formed of an anionic surfactant, a film formed of a nonionic surfactant having an aliphatic hydrocarbon group having 10 to 22 carbon atoms, and an aliphatic hydrocarbon group having 10 to 22 carbon atoms. One or more selected from the group consisting of films formed with amphoteric surfactants.
The surfactant to which the present invention can be applied may be a naturally occurring surfactant. For example, it may be a phospholipid exemplified by phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, phosphatidylinositol, phosphatidylglycerol, and rhamnolipid, sophorolipid, and tre may be glycolipids such as roose lipid. Of these, surfactants containing phospholipids, particularly amphiphilic substances, are particularly preferred.

<Microorganism>
Microbial cell membranes can be used as the surfactant membrane.
The microorganism to which the present invention can be applied can be used for general microorganisms existing in the environment. For example, Moraxella bacteria, Acinetobacter bacteria, Pseudomonas bacteria, Bacillus bacteria, Sphingomonas bacteria, Ralstonia bacteria, Cupriavidas Bacteria belonging to the genus (Cupriavidus), bacteria belonging to the genus Psychorobacter, bacteria belonging to the genus Serratia, bacteria belonging to the genus Escherichia, bacteria belonging to the genus Staphylococcus, bacteria belonging to the genus Burkholderia, and Saccaromyces And yeasts belonging to the genus Rhodotorula.
Specifically, Moraxella sp (Moraxella sp.), Moraxella Osuroenshisu (Moraxella osloensis), Acinetobacter Radio Regis Tense (Acinetobacter radioresistens), Acinetobacter Junii (Acinetobacter JuNii), Acinetobacter calcoaceticus (Acinetobacter calcoaceticus ), Serratia marcescens (Serratia marcescens), Escherichia Korai (Escherichia coli), Staphylococcus aureus (Staphylococcus aureus), Pseudomonas Alcaligenes (Pseudomonas alcaligenes), Bacillus cereus (Bacillus cereus), Bacillus subtilis (Bacillus subtilis), cyclo Arthrobacter Pasi Fi forsythensis (Psychrobacter pacificensis), cyclo Arthrobacter Gurashinkora (Psychrobacter glacincola), Sphingomonas Yanoi Yae (Sphingomonas yanoikuyae), Ralstonia sp (Ralstonia sp.), Saccharomyces cerevisiae (Saccaromyces cerevisiae), Rhodotorula Mushiraginosa (Rhodotorula mucilaginosa), Rhodotorula Surufie (Rhodotorula slooffiae), queue pre-Avi Das oxa Ratih dregs (Cupriavidus oxalaticus) And Burkholderia cepacia .

<How to control odor generation>
In the method for suppressing odor generation of the present invention, the component (a), water and microorganisms are brought into contact with each other to suppress the amount of odorous substances produced by microorganisms. Here, at the pH of the composition containing the component (a) and water, the logD of the component (a) in the composition calculated by the formula (1) is -1.5 or more and 1.5 or less.

The pH of the water containing the component (a) is not particularly limited as long as the logD of the component (a) is within a desired range and the viable cell count of the microorganism can be maintained without killing the microorganism. From the viewpoint of maintaining the viable count of microorganisms, the pH of the composition containing the component (a) and water is preferably 4.0 or more, more preferably 4.5 or more, and still more preferably 5.0. That is all. And from the same viewpoint, the pH of the composition containing the component (a) and water is preferably 10.0 or less, more preferably 9.0 or less, further preferably 8.5 or less, and still more preferably. Is 8.0 or less.
The pH of the present invention can be measured according to a conventional method, for example, with a pH measuring device using a glass electrode.

Ｌｏ The logD of the component (a) calculated by the above formula (1) is -1.5 or more from the viewpoint of further enhancing the metabolic control effect of the microorganism. The logD of the component (a) is preferably -1.2 or more, more preferably -1.0 or more, further preferably -0.8 or more, and still more preferably -0.5 or more. Yes, more preferably −0.2 or more, and even more preferably 0 or more. And from the same viewpoint, the logD of the component (a) is 1.5 or less, preferably 1.3 or less, more preferably 1.1 or less, and further preferably 1.0 or less.

接触 The contact time of the component (a), water and microorganisms can be appropriately determined depending on the degree of controlling the metabolism of microorganisms. From the viewpoint of greater control of the metabolism of the microorganism, the contact time is preferably 1 minute or more, more preferably 5 minutes or more, further preferably 10 minutes or more, and still more preferably 30 minutes or more. And still more preferably 1 hour or more. The contact time is preferably 15 hours or less, more preferably 12 hours or less, and further preferably 8 hours or less.

The odor generation suppressing method of the present invention is preferably a method of suppressing the amount of odorous substances generated by microorganisms while maintaining the viable count of microorganisms after contact with the component (a).
As used herein, "while maintaining the viable cell count of the microorganism" means "the logarithmic value (1) of the viable cell count of the microorganism before contact with the component (a)," It means that the logarithmic value (1) -the logarithmic value (2), which is the difference from the logarithmic value (2) of the viable cell count, is -1 or more and less than 2. The difference in the logarithmic value is generally referred to as a bactericidal activity value, a bacteriostatic activity value, and having bactericidal or having antibacterial properties, respectively, in a bactericidal test or an antibacterial test, the bactericidal activity value Alternatively, it is defined as having a bacteriostatic activity value of 2.2 or more. In the present invention, the bactericidal activity value or the bacteriostatic activity value is -1 or more and less than 2, preferably -0.5 or more and less than 1, more preferably 0 or more and 0.8 or less, further preferably 0 or more and 0.7 or less. One case is referred to as "maintaining the viable microbial count".

臭 The odor that can be suppressed by the present invention can be applied to the odor generated by metabolism of fatty acid synthesis system in the metabolic system of microorganisms. The present invention is a technology that suppresses the generation amount of odorous substances, unlike masking deodorization in which odors generated by metabolism of fatty acid synthesis system are masked to make the odors less noticeable, or chemical deodorization in which odorous substances are changed to odorous substances. is there.

<Odorous substances>
The odor control method of the present invention can be applied to odors generated by metabolism of fatty acid synthesis system in the metabolic system of microorganisms. The present invention masks the odor generated by the metabolism of the fatty acid synthesis system, so that masking deodorization that makes the odor less likely to be perceived, or chemical deodorization that makes the odorous substance less odorable by chemical action, differs from the chemical deodorization that makes the odorous substance less odorable. It is a technique to control.
The odorant is not necessarily limited, but typical odorants include, for example, 4-methyl-3-hexenoic acid (hereinafter also referred to as “4M3H”) consisting of a compound represented by the following formula, or 4-methylhexanoic acid (hereinafter, also referred to as "4MH"). 4-methyl-3-hexenoic acid has cis-trans isomers as shown below, and in the present invention, compounds having both cis- and trans-structures are included.

4M3H and 4MH, which are typical odorous substances, are often produced in the fatty acid synthesis system of microorganisms based on odor precursors contained in sebum stains. "Sebum dirt" is the most typical dirt that adheres to textiles such as clothing, and contains a large amount of oils such as free fatty acids and glycerides, which contain carbon and mud in dust and exfoliated keratin. Is what is observed in textiles and the like. The “sebum dirt component” is not particularly limited as long as it is a sebum dirt component usually found in clothes and the like. Examples of the substance which can be a precursor of the odorous substance include a saturated or unsaturated anteiso fatty acid having 9 to 21 carbon atoms (preferably 11 to 19 carbon atoms, more preferably 17 to 19 carbon atoms) (eg, 6-methyloctane). Acids, 8-methyldecanoic acid, 12-methyltetradecanoic acid, 14-methylhexadecanoic acid, 16-methyloctadecanoic acid, 14-methylhexadecenoic acid and 16-methyloctadecenoic acid), and salts and esters thereof. However, these include compounds that are not actually present in sebum stains.

The odor generation suppressing method of the present invention can be applied to a method in which the component (a), water, and microorganisms come into contact with each other, and the method is not particularly limited by the order in which the components come into contact with each other. For example, a method in which the microorganism containing the component (a) and water contacts the composition containing the component (a), the method in which the component (a) contacts the water and the microorganism, and the composition containing the water and the microorganism where the component (a) contacts, A method in which component (a) contacts water with a microorganism, and a method in which component (a) contacts water in the presence of a microorganism and component (a) contacts water with a microorganism.
As an example of a method in which a composition containing (a) component and water comes into contact with a microorganism, and a component (a) coming into contact with water and a microorganism, for example, a composition containing (a) component and water is sprayed on the microorganism. Alternatively, there may be mentioned a method of applying, and a method of immersing microorganisms in a composition containing the component (a) and water. Examples of a method of spraying the composition include a method of spraying using a sprayer such as a sprayer. As a method of applying the composition, a tool used for application, for example, a microorganism in contact with a sheet-shaped tool made of a cotton fiber or a synthetic fiber containing a composition containing the component (a) and water is contacted. A method of applying a composition containing the component (a) and water to a microorganism.

The state where the component (a), water, and microorganisms are present may be a state of a composition containing the component (a), water, and microorganisms. It may be in the state of a composition containing the same. The target object may be a fiber product, a hard surface, a skin surface, or a hair surface. The material of the fiber product is not particularly limited, and natural materials such as wool, silk, and cotton, and chemical fibers such as polyester and polyamide. , And combinations thereof. In the present invention, the material of the textile is preferably cotton. The fiber product may be unused or used once or more. The fiber product with which the component (a) is brought into contact may be one containing moisture or moisture, or one that has been sufficiently dried. The hard surface may be glass, metal, plastic, pottery.

(4) In order to suppress the generation amount of odorous substances, the amount of the component (a) and water can be appropriately adjusted. For example, when the method for suppressing odor generation of the present invention is carried out on a fiber product, the amount of the component (a) per mass of the fiber product in which microorganisms are present is from 0.01% by mass to 1% by mass. The component (a), water and microorganisms may be brought into contact with water at a pH or an amount at which the component (a) has a specific logD, and the component (a) is brought into contact with water on the textile. The pH of the composition containing component (a) and water when the component (a), water and microorganisms come into contact with each other is adjusted so that the component (a) has a specific logD of the present invention. The component (a) having a logD of -1.5 or more and 1.5 or less in advance if the pH of the water is in advance predictable even when the component (a) comes into contact with water. May be left on the textile.

態 様 As one embodiment of the odor generation suppressing method of the present invention, there is an odor generation suppressing method in which a sheet comprising a nonwoven fabric, which contains the component (a) and water, is brought into contact with an object to which microorganisms are attached. In this embodiment, at the pH of the composition containing the component (a) and water, the logD of the component (a) calculated by the formula (1) is −1.5 or more and 1.5 or less.

用 As a tool that can be suitably used in the method for suppressing odor generation of the present invention, a sheet containing a component (a) and water and made of a nonwoven fabric may be mentioned. The logD of the component (a) calculated from the above formula (1) at the pH of the composition containing the component (a) and water is −1.5 or more and 1.5 or less.

事項 Items relating to the optional components such as component (a), water, formulas (1) and (b) used in the sheet of the present invention can be the same as those described in the above-mentioned method for suppressing odor generation.

In the sheet of the present invention, the nonwoven fabric is processed into a sheet shape, and the fibers constituting the nonwoven fabric are formed of one or more fibers selected from the group consisting of hydrophilic fibers and hydrophobic fibers. preferable.
In the present invention, the term “hydrophilic fiber” refers to a fiber having a moisture content (20 ° C., 65% RH) in a standard state of more than 5%. The moisture content in the standard state is measured by a method specified in JIS L 1013 and JIS L 1015. The “hydrophobic fiber” refers to a fiber having a standard moisture content (20 ° C., 65% RH) of 5% by weight or less.

Examples of the chemical fiber which is a hydrophobic fiber that can be used in the sheet of the present invention include polyamide fibers (eg, nylon), polyester fibers (eg, polyester), polyacrylonitrile fibers (eg, acrylic), and polyvinyl alcohol fibers. (Vinylon, etc.), polyvinyl chloride fiber (polyvinyl chloride, etc.), polyvinylidene chloride fiber (vinylidene, etc.), polyolefin fiber (polyethylene, polypropylene, etc.), polyurethane fiber (polyurethane, etc.), polyvinyl chloride / polyvinyl Examples thereof include alcohol copolymer fibers (such as polychloral), and one or more of these can be used.
The hydrophilic fibers that can be used in the sheet of the present invention include seed hair fibers (cotton, noodles, kapok, etc.), bast fibers (hemp, flax, ramie, cannabis, jute, etc.), and vein fibers (manila hemp, sisal hemp). ), Palm fiber, rush, straw, animal hair fiber (wool, mohair, cashmere, camel, alpaca, bikuna, angora, etc.), silk fiber (domestic silk, wild silk), feather, cellulose fiber (rayon, Polynosic, cupra, acetate, etc.), hydrophilized polyethylene terephthalate fibers (polyethylene terephthalate fibers that have been subjected to hydrophilization treatment and fibers having a water content in the standard state within the range of the hydrophilic fibers described above), and the like. Species or two or more species can be used.

The nonwoven fabric used in the present invention preferably contains hydrophilic fibers. The nonwoven fabric used in the present invention contains hydrophilic fibers in an amount of 50% by mass or more, more preferably 60% by mass or more, further preferably 70% by mass or more, and still more preferably 75% by mass or more, from the viewpoint of further enhancing the effect of suppressing odor generation. Is preferable. The content of the hydrophilic fiber contained in the nonwoven fabric is preferably 100% by mass or less, more preferably 90% by mass or less, and further preferably 85% by mass or less.
Further, from the viewpoint of further enhancing the effect of suppressing the generation of odor, the basis weight of the nonwoven fabric used in the present invention is preferably 10 g / m ² or more, more preferably 20 g / m ² or more. The basis weight of the nonwoven fabric used in the present invention is preferably 100 g / m ² or less, more preferably 80 g / m ² or less, and further preferably 60 g / m ² or less.

The mass ratio (impregnation rate (% by mass)) of the composition containing the component (a) and water to the mass of the nonwoven fabric is preferably 100% by mass or more, more preferably 150% by mass, from the viewpoint of further enhancing the effect of suppressing odor generation. % By mass, more preferably at least 200% by mass, more preferably at least 250% by mass, further preferably at least 300% by mass, still more preferably at least 350% by mass. And from the same viewpoint, the mass ratio (impregnation rate (% by mass)) of the composition containing the component (a) and water with respect to the mass of the nonwoven fabric is preferably 500% by mass or less, more preferably 420% by mass or less, It is more preferably at most 380% by mass, further preferably at most 350% by mass.
In the present invention, the impregnation rate is calculated from the following equation.

Impregnation rate (% by mass) =
((Mass of nonwoven fabric impregnated with composition containing component (a) and water) / (mass of dried nonwoven fabric) -1) × 100

The content of the component (a) with respect to the mass of the composition containing the component (a) and water, which is contained in the sheet of the present invention, is preferably 0.01% by mass or more from the viewpoint of further enhancing the effect of suppressing odor generation. Yes, more preferably at least 0.03% by mass, even more preferably at least 0.05% by mass, even more preferably at least 0.08% by mass. And from the same viewpoint, the content of the component (a) is preferably 1% by mass or less, more preferably 0.8% by mass or less, and further preferably 0.5% by mass or less.

By bringing the sheet of the present invention into contact with an object to which microorganisms have adhered, it is possible to suppress generation of odor from the object to which microorganisms have adhered.
The object to which the microorganisms have adhered may be any of a textile, a hard surface, a skin surface, and a hair surface. The material of the fiber product is not particularly limited, and may be any of natural materials such as wool, silk and cotton, chemical fibers such as polyester and polyamide, and combinations thereof. In the present invention, the material of the textile is preferably cotton. The fiber product may be unused or used once or more. The fiber product with which the component (a) is brought into contact may be one containing moisture or moisture, or one that has been sufficiently dried. The hard surface may be glass, metal, plastic, pottery.

<PH control method>
In the pH control of the present invention, in two phases containing water separated by a surfactant membrane, the logD of the component (a) present in one phase, which is calculated by the formula (1), is -1.5. By controlling the pH to 1.5 or less, the pH of the other phase separated by the surfactant film can be reduced to control the pH.

The pH of the water containing the component (a) is not particularly limited as long as the logD of the component (a) is in the range of -1.5 to 1.5 and the pH is such that a surfactant film can be present. Absent. The pH of the composition containing the component (a) and water is preferably 4.0 or more, and more preferably, from the viewpoint that the pH of the internal phase separated by the surfactant membrane, particularly the cell membrane of the microorganism, can be further reduced. It is 4.5 or more, more preferably 5.0 or more. And from the same viewpoint, the pH of the composition containing the component (a) and water is preferably 10.0 or less, more preferably 9.0 or less, further preferably 8.5 or less, and still more preferably. Is 8.0 or less.
The pH of the present invention can be measured according to a conventional method, for example, with a measuring device using a glass electrode.

Ｌｏ The logD of the component (a) calculated by the above formula (1) is -1.5 or more from the viewpoint of further reducing the pH of the internal phase separated by the surfactant membrane, especially the cell membrane of the microorganism. The logD of the component (a) is preferably -1.2 or more, more preferably -1.0 or more, further preferably -0.8 or more, and still more preferably -0.5 or more. Yes, more preferably −0.2 or more, and even more preferably 0 or more. From the same viewpoint, logD of the component (a) is 1.5 or less, preferably 1.3 or less, more preferably 1.1 or less, and further preferably 1.0 or less.

接触 The contact time of the component (a), water and a surfactant membrane, especially a cell membrane of a microorganism, can be appropriately determined depending on the degree to which the pH is controlled. From the viewpoint of increasing the control of pH, the contact time is preferably 1 minute or more, more preferably 5 minutes or more, further preferably 10 minutes or more, and still more preferably 30 minutes or more. More preferably, it is one hour or more. The contact time is preferably 15 hours or less, more preferably 12 hours or less, and further preferably 8 hours or less.

に 関 し Regarding the above-described embodiment, the present invention further discloses the following method and sheet.

<1>
The following (a) component, water and microorganisms are brought into contact with each other to suppress the amount of odorous substances produced by the microorganisms,
An odor generation suppressing method, wherein the component (a) has a logD of -1.5 or more and 1.5 or less, calculated by the following formula (1), at the pH of the composition containing the component (a) and water.

Component (a): organic carboxylic acid or salt thereof
logD = ClogP-log [1 + 10 ^(pH-pKa) ] Formula (1)
[In the formula (1), ClogP is a calculated logP of the logarithmic value of the water / octanol partition coefficient in the acid form of the component (a), pH is the pH of water containing the component (a), and pKa is PKa of the component (a). ]

<2>
The method according to <1>, wherein the odorant is at least one selected from the group consisting of 4-methyl-3-hexenoic acid and 4-methylhexanoic acid.
<3>
The above-mentioned odor generation suppressing method comprises the steps of: (a) a method in which a composition containing component (a) and water is brought into contact with a microorganism; (a) a method in which component, water and a microorganism are brought into contact; The method comprises contacting the components and bringing the component (a) into contact with water and microorganisms, and contacting the component (a) with water in the presence of the microorganisms and contacting the component (a) with water and the microorganisms. The method according to <1> or <2>, which is one or more methods selected from the group.
<4>
(A) The method according to any one of <1> to <3>, wherein the sheet comprising a nonwoven fabric, which contains the component and water, is brought into contact with an object to which microorganisms have adhered. The above (1) to (3), wherein logD of the component (a) calculated by the formula (1) at the pH of the composition containing the component and water is −1.5 or more and 1.5 or less. A method according to any one of the preceding claims.

<5>
In two phases containing water separated by a surfactant film, the logD of the component (a) present in one phase, which is calculated by the formula (1), is controlled to be -1.5 or more and 1.5 or less. And controlling the pH of the other phase separated by the surfactant film.

<6>
The surfactant film is a film formed of a cationic surfactant having an aliphatic hydrocarbon group having 10 to 22 carbon atoms, anionic surfactant having an aliphatic hydrocarbon group having 10 to 22 carbon atoms. Film formed of a nonionic surfactant having an aliphatic hydrocarbon group having 10 to 22 carbon atoms, and an amphoteric interface having an aliphatic hydrocarbon group having 10 to 22 carbon atoms. <5> The method according to <5>, wherein the method is a membrane formed with an activator, preferably a cell membrane of a microorganism.

<7>
The value represented by (molecular weight of component (a)) / (molecular weight of carboxyl group) is 1.9 or more, preferably 2.0 or more, more preferably 2.2 or more, and is 3.5 or less, preferably Is 3.2 or less, more preferably 3.0 or less, and even more preferably 2.8 or less, the method according to any one of the above items <1> to <6>.
<8>
The component (a) is a group consisting of aliphatic polyhydric carboxylic acids having 8 or more, preferably 9 or more, and 14 or less, preferably 12 or less, more preferably 10 or less carbon atoms, and salts thereof. The method according to any one of the above <1> to <7>, wherein the method is one or more compounds selected from the group consisting of:
<9>
The component (a) is one or more compounds selected from the group consisting of aliphatic dicarboxylic acids and salts thereof, preferably suberic acid, azelaic acid, sebacic acid, dodecandioic acid, tetradecandioic acid and salts thereof. The method according to any one of the above <1> to <8>, wherein the method is one or more compounds selected from the group consisting of:

<10>
The method according to any one of the above items <1> to <6>, wherein the component (a) is an organic carboxylic acid having one naphthalene group and one carboxyl group or a salt thereof.
<11>
Wherein the organic carboxylic acid or a salt thereof is 1-naphthalene carboxylic acid, 2-naphthalene carboxylic acid, 2-hydroxy-1-naphthalene carboxylic acid, 3-hydroxy-2-naphthalene carboxylic acid, 6-hydroxy-2-naphthalene carboxylic acid And the at least one compound selected from the group consisting of salts thereof.

<12>
The logD of the component (a) calculated by the above formula (1) at the pH of the composition containing the component (a) and water is -1.2 or more, preferably -1.0 or more, more preferably -0. 0.8 or more, more preferably -0.5 or more, still more preferably -0.2 or more, still more preferably 0 or more, 1.3 or less, preferably 1.1 or less, more preferably 1.0 or less. The method according to any one of the above items <1> to <11>.
<13>
(A) The method according to any one of <1> to <12>, wherein the viable cell count of the microorganism after contact with the component is maintained.
<14>
Difference between the logarithmic value (1) of the viable cell count of the microorganism before contact with the component (a) and the logarithmic value (2) of the viable cell count of the microorganism after contact with the component (a) (logarithmic value (1)) <13> wherein the (logarithmic value (2)) is -1 or more and less than 2, preferably -0.5 or more and less than 1, more preferably 0 or more and 0.8 or less, and still more preferably 0 or more and 0.7 or less. The method described in.
<15>
The pH of the composition containing the component (a) and water is 4.0 or more, preferably 4.5 or more, more preferably 5.0 or more, and 10.0 or less, preferably 9.0 or less. The method according to any one of the above items <1> to <14>, wherein the method is preferably 8.5 or less, more preferably 8.0 or less.
<16>
The method according to any one of the above items <1> to <15>, wherein the pH of the composition containing the component (a) and water is 5.0 or more and 8.5 or less.
<17>
(A) The contact time of the component, water and a surfactant film, especially a microorganism, is 1 minute or more, preferably 5 minutes or more, more preferably 10 minutes or more, still more preferably 30 minutes or more, and even more preferably 1 hour. The method according to any one of <1> to <16> above, wherein the time is 15 hours or less, preferably 12 hours or less, more preferably 8 hours or less.
<18>
The content of the component (a) with respect to the mass of the composition containing the component (a) and water is 0.01% by mass or more, preferably 0.03% by mass or more, more preferably 0.05% by mass or more, and furthermore Any one of the above <1> to <17>, which is preferably 0.08% by mass or more and 1% by mass or less, preferably 0.8% by mass or less, more preferably 0.5% by mass or less. The described method.

<19>
(A) A sheet comprising a nonwoven fabric, containing a component and water,
The sheet wherein the logD of the component (a) calculated by the above formula (1) at the pH of the composition containing the component (a) and water is −1.5 or more and 1.5 or less.

<20>
The value represented by (molecular weight of component (a)) / (molecular weight of carboxyl group) is 1.9 or more, preferably 2.0 or more, more preferably 2.2 or more, and is 3.5 or less, preferably Is not more than 3.2, more preferably not more than 3.0, and still more preferably not more than 2.8.
<21>
The component (a) is a group consisting of aliphatic polyhydric carboxylic acids having 8 or more, preferably 9 or more, and 14 or less, preferably 12 or less, more preferably 10 or less carbon atoms, and salts thereof. The sheet according to <19> or <20>, which is one or more compounds selected from the group consisting of:
<22>
The component (a) is one or more compounds selected from the group consisting of aliphatic dicarboxylic acids and salts thereof, preferably suberic acid, azelaic acid, sebacic acid, dodecandioic acid, tetradecandioic acid and salts thereof. The sheet according to any one of the above items <19> to <21>, wherein the sheet is one or more compounds selected from the group consisting of:

<23>
The sheet according to <19>, wherein the component (a) is an organic carboxylic acid having one naphthalene group and one carboxyl group or a salt thereof.
<24>
Wherein the organic carboxylic acid or a salt thereof is 1-naphthalene carboxylic acid, 2-naphthalene carboxylic acid, 2-hydroxy-1-naphthalene carboxylic acid, 3-hydroxy-2-naphthalene carboxylic acid, 6-hydroxy-2-naphthalene carboxylic acid And the at least one compound selected from the group consisting of salts thereof.

<25>
The logD of the component (a) calculated by the above formula (1) at the pH of the composition containing the component (a) and water is -1.2 or more, preferably -1.0 or more, more preferably -0. 0.8 or more, more preferably -0.5 or more, still more preferably -0.2 or more, still more preferably 0 or more, 1.3 or less, preferably 1.1 or less, more preferably 1.0 or less. The sheet according to any one of the above items <19> to <24>.
<26>
The pH of the composition containing the component (a) and water is 4.0 or more, preferably 4.5 or more, more preferably 5.0 or more, and 10.0 or less, preferably 9.0 or less. The sheet according to any one of the above items <19> to <25>, wherein the sheet is preferably 8.5 or less, more preferably 8.0 or less.
<27>
The sheet according to any one of the items <19> to <26>, wherein the pH of the composition containing the component (a) and water is 5.0 or more and 8.5 or less.

<28>
The sheet according to any one of the items <19> to <27>, wherein the nonwoven fabric is composed of one or more kinds of fibers selected from the group consisting of hydrophilic fibers and hydrophobic fibers.
<29>
The hydrophobic fibers are polyamide fibers, polyester fibers, polyacrylonitrile fibers, polyvinyl alcohol fibers, polyvinyl chloride fibers, polyvinylidene chloride fibers, polyolefin fibers, polyurethane fibers, and polyvinyl chloride / polyvinyl. The sheet according to <28>, wherein the sheet is one or more fibers selected from the group consisting of alcohol copolymer fibers.
<30>
The hydrophilic fiber is selected from the group consisting of seed hair fiber, bast fiber, vein fiber, palm fiber, rush, straw, animal hair fiber, silk fiber, feather, cellulosic fiber, and hydrophilized polyethylene terephthalate fiber. The sheet according to <28>, wherein the sheet is one or two or more fibers.
<31>
The nonwoven fabric contains the hydrophilic fiber in an amount of 50% by mass or more, preferably 60% by mass or more, more preferably 70% by mass or more, still more preferably 75% by mass or more, and 100% by mass or less, more preferably 90% by mass or less. The sheet according to any one of the above <28> to <30>, preferably containing 85% by mass or less.
<32>
The nonwoven fabric has a basis weight of 10 g / m ² or more, preferably 20 g / m ² or more, 100 g / m ² or less, preferably 80 g / m ² or less, more preferably 60 g / m ² or less. > The sheet according to any one of <31>.
<33>
The impregnation ratio (% by mass) of the composition containing the component (a) and water with respect to the mass of the nonwoven fabric is 100% by mass or more, preferably 150% by mass or more, more preferably 200% by mass or more, more preferably 250% by mass or more. % By mass or more, more preferably 300% by mass or more, still more preferably 350% by mass or more, 500% by mass or less, preferably 420% by mass or less, more preferably 380% by mass or less, and still more preferably 350% by mass or less. The sheet according to any one of <19> to <32>.
<34>
The content of the component (a) with respect to the mass of the composition containing the component (a) and water is 0.01% by mass or more, preferably 0.03% by mass or more, more preferably 0.05% by mass or more, and furthermore Any one of the above items <19> to <33>, which is preferably 0.08% by mass or more and 1% by mass or less, preferably 0.8% by mass or less, more preferably 0.5% by mass or less. The described sheet.
<35>
The sheet according to any one of the above items <19> to <34>, which is used for suppressing generation of an odor from an object to which microorganisms have adhered by bringing the object into contact with the object to which microorganisms have adhered.
<36>
The sheet according to <35>, wherein the object to which the microorganism is attached is any one of a fiber product, a hard surface, a skin surface, and a hair surface.

Hereinafter, the present invention will be described in more detail with reference to Examples, but the present invention is not limited thereto.

Example 1
(1) Method of quantifying odor-causing substances A methanol solution containing 14-methylhexadecanoic acid (SIGMA-ALDRICH), a precursor of odor-causing substances, was dropped onto 3 x 3 cm of cotton plain woven cloth sterilized from the day before the test. Then, the precursor of the odor-causing substance was attached. The attached amount of the precursor of the odor-causing substance was set so that the fatty acid was 100 μg (1,000 μg / g cloth) per cotton plain woven cloth. Further, a methanol solution (1 mM) in which various organic acids shown in Table 1 were dissolved was prepared, and 100 μL was dropped on each cloth to which the substrate was adhered, and dried overnight.
From the previous day, Moraxella osloensis ATCC 19976 strain was cultured under agar culture conditions, and the cells were collected in sterile ion-exchanged water. OD ₆₀₀ = 0.1 (10 ⁸ CFU / mL), 1 / 2NB (Nutrient Broth: Difco) Was prepared.
Put the cloth in which the precursor of the odor-causing substance and various organic acids are present in a sterilized No. 3 screw tube (Maruemu), inoculate 100 μL of the bacterial suspension, and incubate at 37 ° C. for 8 hours. Culture was performed. The same operation was performed under the condition that no bacteria were present, and the pH of the cloth (precursor of the odor-causing substance, presence of the organic acid) was measured before culturing.

After the culture, extraction was performed by adding 3 mL of methanol and applying ultrasonic waves for 30 minutes. The extract was mixed with ADAM reagent (0.5 mg / mL MeOH solution) from Funakoshi at a ratio of 1: 1 (volume ratio) and allowed to stand overnight at room temperature in the dark. Thereafter, 4-methyl-3-hexenoic acid and 4-methylhexanoic acid were quantified using HPLC. The quantitative conditions for 4-methyl-3-hexenoic acid and 4-methylhexanoic acid are shown below.

.Quantification conditions for 4-methyl-3-hexenoic acid and 4-methylhexanoic acid
LC column: Zorbax C8 4.6 x 250 mm
Eluent: 61% (v / v) acetonitrile, 39% (v / v) water
Column temperature: 40 ° C
Sample injection volume: 10 μL
Flow rate: 1.0 mL / min
Detection: FID Ex. 365 nm, Em. 412 nm

(2) Test method for change in bacterial count (antibacterial test method)
A cloth prepared in the same manner as the above-mentioned method for quantifying an odor-causing substance is placed in a sterilized No. 3 screw tube (Maruemu), inoculated with 100 μL of the bacterial solution, and cultured at 37 ° C. for 8 hours. Was done. After the culture, 20 mL of LP diluent (manufactured by Nippon Pharmaceutical Co., Ltd.) was added, and the mixture was irradiated with ultrasonic waves for 10 minutes to extract the bacteria.

The extract was serially diluted, pulverized on an SCD-LP agar medium (manufactured by Nippon Pharmaceutical Co., Ltd.), and cultured at 37 ° C. for 1 day. The number of colonies obtained for each cloth was counted, and the common logarithm of the viable cell count of each Example or Comparative Example was subtracted from the common logarithm of the viable cell count of the cloth that had not been washed (logarithmic value). ) Was taken as the bacteriostatic activity value.
The common logarithm of the bacterial count (CFU / cm ² ) before the test was 6.1 (X1). When (X2-X1), which is a difference from a common logarithmic value (referred to as X2) of the number of bacteria (CFU / cm ² ) after the culture test, is not less than −1 and less than 2, a state in which the number of bacteria is maintained. I can say. The pH of the cloth (precursor of odor-causing substance, presence of organic acid) was measured before culturing.
Table 1 shows the above results.

As shown in Table 1, when the logD of the organic carboxylic acid to be brought into contact with the microorganism is -1.5 or more and 1.5 or less, the generation of the odor-causing substance is suppressed without sterilizing the microorganism causing the odor. (See Examples 1-1 to 1-4).
On the other hand, when the logD of the organic carboxylic acid was out of the range specified in the present invention, generation of the odor-causing substance could not be suppressed (see Comparative Examples 1-2 to 1-5).

Example 2 Evaluation method of pH change In Examples, a change in pH was evaluated using a cell membrane of a microorganism as a representative example of a surfactant membrane. That is, the pH of the component (a) and water that come into contact with the microorganism was appropriately selected, the logD of the component (a) was controlled, and the pH was changed in the cytoplasm of the microorganism by contacting the microorganism. The pH change in the cytoplasm was evaluated using Life Technologies' pHrodo (R) Green AM Intracellular pH Indicators kit.

The Moraxella osloensis ATCC 19976 strain cultured from the previous day was suspended in Live Cell Imaging Solution (Life Technologies, hereinafter referred to as “LCIS”) and washed by centrifugation (5600 rpm, 10 minutes, 4 ° C.). . After washing, 5 mL of a bacterial solution having an OD _{600 of} 10 was prepared, and centrifuged again to collect bacterial cells.
5 μL of pH rodo dye was added to 50 μL of powerload, and after stirring, 5 mL of LCIS was added. 5 mL of the obtained mixed solution was added to the collected cell pellets to suspend the cells, and the cells were allowed to stand at 37 ° C for 1 hour. After standing, the mixture was centrifuged, washed once with 5 mL of LCIS, and then 5 mL of LCIS was added again. The solution subjected to the fluorescent staining treatment is referred to as a bacterial solution A.
To the fluorescence measurement cell, 2.4 mL of a phosphate buffer (55 mM, pH 6), 0.3 mL of bacterial solution A, and 0.3 mL of a sodium salt aqueous solution of various organic acids shown in Table 2 were added, and the fluorescence was measured immediately after stirring. (Excitation wavelength 509 nm, fluorescence wavelength 533 nm). The higher the fluorescence intensity, the more acidic the cytoplasmic environment.
Table 2 shows the results.

As shown in Table 2, when the logD of the organic carboxylic acid brought into contact with the microorganism was -1.5 or more and 2 or less, the value of the fluorescence intensity increased, and the pH in the cell membrane of the microorganism decreased (Example 2-1). ２2-3).
On the other hand, when the logD of the organic carboxylic acid was out of the range specified in the present invention, there was no change in the value of the fluorescence intensity, and the pH in the cell membrane of the microorganism could not be lowered (Comparative Example 1-2). To 1-5).

Example 3
The same operation as in Example 1 was performed except that the compounds shown in Table 3 were used, and the quantification of 4-methyl-3-hexenoic acid and the measurement of the number of bacteria were performed. Table 3 shows the results.

Note that the amount of 4M3H on the cloth differs between Comparative Example 3-1 and Comparative Example 1-1. This is presumed that the active state of the fatty acid metabolism system in the cells varies depending on individual differences.
As shown in Table 3, the rate of decrease in the amount of 4M3H in each of Examples 3-1 to 3-4 was higher than that in Comparative Example 3-1. In the case of -1.5 or more and 1.5 or less, generation of the odor-causing substance could be suppressed without sterilizing microorganisms that cause odor.
On the other hand, when the logD of the organic carboxylic acid was out of the range specified in the present invention, generation of the odor-causing substance could not be suppressed (see Comparative Example 3-2).

Example 4
The compositions described in Table 4 below were prepared. The pH was adjusted using a 1% by mass aqueous sodium hydroxide solution.

The composition described in Table 4 was applied to a nonwoven fabric (sheet-like spunlace nonwoven fabric (rayon / polypropylene / polyester ratio = 80/10/10, basis weight 60 g / m ² , 150 mm × 100 mm)) and the mass of the nonwoven fabric The composition was impregnated so as to have an impregnation rate of 350% by mass to prepare a sheet for textile products.

(1) Method of quantifying odor-causing substances A methanol solution containing 14-methylhexadecanoic acid (SIGMA-ALDRICH), a precursor of odor-causing substances, was dropped onto 3 x 3 cm of cotton plain woven cloth sterilized from the day before the test. Then, the precursor of the odor-causing substance was attached. The attached amount of the precursor of the odor-causing substance was set so that the fatty acid was 100 μg (1,000 μg / g cloth) per cotton plain woven cloth. From the previous day, Moraxella osloensis ATCC 19976 strain was cultured under agar culture conditions, and the cells were collected in sterile ion-exchanged water. OD ₆₀₀ = 0.1 (10 ⁸ CFU / mL), 1 / 2NB (Nutrient Broth: Difco) Was prepared.
The cloth to which the precursor of the odor-causing substance adhered was placed in a sterilized No. 3 screw tube (Maruemu), and 100 μL of the above-mentioned bacterial suspension was inoculated. Further, the textile sheet was brought into contact with the cloth, allowed to stand for 5 minutes, separated from the cloth, and dried overnight.
When the pH of the liquid between the cloth and the sheet for textiles was measured with a pH meter of a glass electrode, the pH was 6 in all cases.
After the culture, the amount of 4-methyl-3-hexenoic acid was quantified under the same conditions as in Example 1.

(2) Test method for change in bacterial count (antibacterial test method)
A cloth left overnight by the same method as the above-mentioned method for quantifying an odor-causing substance is placed in a sterilized No. 3 screw tube (Maruem), and 20 mL of an LP diluent (manufactured by Nippon Pharmaceutical Co., Ltd.) is added thereto, and ultrasonic waves are applied. Irradiation was performed for 10 minutes to extract bacteria.
The bacteriostatic activity value of the obtained extract was measured in the same manner as in Example 1. The pH of the cloth (precursor of odor-causing substance, presence of organic acid) was measured before culturing.
Table 5 shows the above results.

As shown in Table 5, when the logD of the organic carboxylic acid to be brought into contact with the microorganism is -1.5 or more and 1.5 or less, the generation of the odor-causing substance is suppressed without sterilizing the microorganism causing the odor. (See Example 4-1).
On the other hand, when the logD of the organic carboxylic acid was out of the range specified in the present invention, generation of the odor-causing substance could not be suppressed (see Comparative Example 4-1).

Although the present invention has been described in conjunction with its embodiments, we do not intend to limit our invention in any detail of the description unless otherwise specified, which is contrary to the spirit and scope of the invention as set forth in the appended claims. I think it should be interpreted broadly without.

This application claims the priority based on Japanese Patent Application No. 2018-132966, filed in Japan on July 13, 2018, which is hereby incorporated by reference. Capture as a part.

Claims (18)

1. The following (a) component, water and microorganisms are brought into contact with each other to suppress the amount of odorous substances produced by the microorganisms,
   An odor generation suppressing method, wherein the component (a) has a logD of -1.5 or more and 1.5 or less, calculated by the following formula (1), at the pH of the composition containing the component (a) and water.
   
   Component (a): organic carboxylic acid or salt thereof
   logD = ClogP-log [1 + 10 ^(pH-pKa) ] Formula (1)
   [In the formula (1), ClogP is a calculated logP value of a logarithmic value of a water / octanol partition coefficient in the acid form of the component (a), pH is the pH of water containing the component (a), and pKa Is the pKa of the component (a). ]
2. The method according to claim 1, wherein the odorant is at least one selected from the group consisting of 4-methyl-3-hexenoic acid and 4-methylhexanoic acid.
3. 3. The method according to claim 1, wherein the sheet comprising the nonwoven fabric, which contains the component (a) and water, is brought into contact with an object to which microorganisms have adhered, and the composition comprises the component (a) and water. 4. The method for suppressing odor generation according to claim 1 or 2, wherein logD of the component (a) calculated by the formula (1) at a pH of from -1.5 to 1.5.
4. In two phases containing water separated by a surfactant film, the logD calculated by the following formula (1) of the following component (a) present in one phase is controlled to be -1.5 or more and 1.5 or less. And controlling the pH of the other phase separated by the surfactant film.
   
   Component (a): organic carboxylic acid or salt thereof
   logD = ClogP-log [1 + 10 ^(pH-pKa) ] Formula (1)
   [In the formula (1), ClogP is a calculated logP value of a logarithmic value of a water / octanol partition coefficient in the acid form of the component (a), pH is the pH of water containing the component (a), and pKa Is the pKa of the component (a). ]
5. The pH control method according to claim 4, wherein the surfactant film is a cell membrane of a microorganism.
6. The method according to any one of claims 1 to 5, wherein the component (a) is at least one compound selected from the group consisting of aliphatic polycarboxylic acids having 8 to 14 carbon atoms and salts thereof. The described method.
7. 7. The method according to claim 1, wherein the component (a) is at least one compound selected from the group consisting of suberic acid, azelaic acid, sebacic acid, dodecandioic acid, tetradecandioic acid, and salts thereof. The method described in the section.
8. 方法 The method according to any one of claims 1 to 5, wherein the component (a) is an organic carboxylic acid having one naphthalene group and one carboxyl group or a salt thereof.
9. Wherein the organic carboxylic acid or a salt thereof is 1-naphthalene carboxylic acid, 2-naphthalene carboxylic acid, 2-hydroxy-1-naphthalene carboxylic acid, 3-hydroxy-2-naphthalene carboxylic acid, 6-hydroxy-2-naphthalene carboxylic acid The method according to claim 8, wherein the compound is at least one compound selected from the group consisting of:
10. The method according to any one of claims 1 to 9, wherein the viable cell count of the microorganism after contact with the component (a) is maintained.
11. (11) The method according to any one of (1) to (10), wherein the composition containing the component (a) and water has a pH of 5.0 or more and 8.5 or less.
12. (A) A sheet comprising a nonwoven fabric, containing a component and water,
    A sheet wherein the logD of the component (a) calculated by the following formula (1) at a pH of the composition containing the component (a) and water is from −1.5 to 1.5.
    
    Component (a): organic carboxylic acid or salt thereof
    logD = ClogP-log [1 + 10 ^(pH-pKa) ] Formula (1)
    [In the formula (1), ClogP is a calculated logP value of a logarithmic value of a water / octanol partition coefficient in the acid form of the component (a), pH is the pH of water containing the component (a), and pKa Is the pKa of the component (a). ]
13. The sheet according to claim 12, wherein the component (a) is at least one compound selected from the group consisting of aliphatic polycarboxylic acids having 8 to 14 carbon atoms and salts thereof.
14. 14. The sheet according to claim 12, wherein the component (a) is at least one compound selected from the group consisting of suberic acid, azelaic acid, sebacic acid, dodecandioic acid, tetradecandioic acid, and salts thereof. 15. .
15. The sheet according to claim 12, wherein the component (a) is an organic carboxylic acid having one naphthalene group and one carboxyl group or a salt thereof.
16. Wherein the organic carboxylic acid or a salt thereof is 1-naphthalene carboxylic acid, 2-naphthalene carboxylic acid, 2-hydroxy-1-naphthalene carboxylic acid, 3-hydroxy-2-naphthalene carboxylic acid, 6-hydroxy-2-naphthalene carboxylic acid The sheet according to claim 15, wherein the sheet is at least one compound selected from the group consisting of: and a salt thereof.
17. 17. The method according to claim 12, wherein an impregnation ratio (% by mass) of the composition containing the component (a) and water with respect to the mass of the nonwoven fabric is 100% by mass or more and 500% by mass or less. Sheet.
18. The sheet according to any one of claims 12 to 17, wherein the content of the component (a) is 0.01% by mass or more and 1% by mass or less based on the mass of the composition containing the component (a) and water. .