TWI829815B - Antibacterial and antiviral compositions, aqueous solutions, soaps, sanitary products, residential detergents, kitchen detergents, laundry detergents, residential antibacterial and antiviral agents, kitchen antibacterial and antiviral agents, clothing antibacterial and antiviral agents, Cosmetics, wet wipes and wet handkerchiefs - Google Patents

Abstract

Provided are an antibacterial and antiviral composition, aqueous solution, soap, sanitary products, residential detergent, kitchen detergent, clothing detergent, residential antibacterial and antiviral agent, kitchen antibacterial and antiviral agent, and clothing antibacterial and disease resistance Poisons, cosmetics, wet wipes and wet handkerchiefs, including at least one of VOSO ₄ , K ₁₁ H [(VO) ₃ (SbW ₉ O ₃₃ ) ₂ ] and Na ₉ (SbW ₉ O ₃₃ ), and polyhexamethylene Biguanide or its salt.

Description

Antibacterial and antiviral compositions, aqueous solutions, soaps, hygiene products, residential detergents, kitchen detergents, laundry detergents, residential antibacterial and antiviral agents, kitchen antibacterial and antiviral agents, clothing antibacterial and antiviral agents, cosmetics, wet wipes and wet handkerchiefs

The present invention relates to an antibacterial and antiviral composition with antibacterial activity and antiviral activity and an aqueous solution containing the antibacterial and antiviral composition.

In recent years, as hygiene awareness has increased, antibacterial substances have been included in daily necessities and the like in order to improve the hygiene around the kitchen (for example, see Patent Document 1). In addition, in order to prevent infectious diseases such as influenza, antiviral substances having antiviral activity are included in daily necessities and the like (for example, see Patent Document 2).

In addition, wet handkerchiefs such as rental wet handkerchiefs that mainly use fiber products such as towels, and disposable wet handkerchiefs that use paper or non-woven fabrics may have bacteria and viruses attaching and multiplying depending on the use environment or storage environment. Therefore, hygiene management and infectious disease prevention measures are also important issues for wet handkerchiefs.

For such bacteria and viruses, parabens or high-concentration alcohol are also considered, but they may also cause skin roughness. Therefore, Patent Document 3 describes a water-soluble solution using an alkali metal salt of ε-polylysine acid and sorbic acid. Disposable wet handkerchief with liquid and anti-mold effect (for example, refer to Patent Document 3).

In addition, in cosmetics, in order to prevent deterioration caused by the contamination of bacteria, mold and other bacteria during production and use, antibacterial agents that are less irritating to the skin than parabens are being studied (for example, see Patent document 4).

[Prior art documents]

[Patent Document]

[Patent Document 1] Japanese Patent Application Publication No. 2014-139164

[Patent Document 2] Japanese Patent Application Publication No. 2009-155262

[Patent Document 3] Japanese Patent Application Laid-Open No. 2009-149575

[Patent Document 4] Japanese Patent Application Publication No. 2008-63271

However, in Patent Document 1, in order to prepare the antibacterial substance into an aqueous solution, there is a step of processing at a high temperature of 110°C to 180°C, so the steps become complicated and there is a problem of increasing manufacturing costs. In addition, Patent Document 2 uses a suspension in which particles containing silica and/or particles containing alumina are dispersed in water. Therefore, stirring is required during use, and it takes effort to use it as an antiviral agent.

In addition, the disposable wet handkerchief of Patent Document 3 may have various bacteria and viruses adhering to and multiplying depending on the use environment and storage environment. Therefore, it is desired to have antibacterial activity against various bacteria and antiviral activity against various viruses.

In addition, rental wet handkerchiefs are used at rental locations such as restaurants, showrooms, offices, hairdressing and beauty salons, etc., and are provided to users at the rental locations. Also, rent wet hands Handkerchiefs are recycled after use and used again after washing. In terms of cleaning, the Ministry of Health, Labor and Welfare has formulated "Guidance Standards on Hygienic Handling of Wet Handkerchiefs, etc." and requires that the number of bacteria after washing be below the standard value.

However, since rental wet handkerchiefs are used in various environments, there is a risk that various bacteria and viruses may attach to the rental wet handkerchiefs and multiply. Therefore, the rental of wet handkerchiefs not only requires specific bacteria and viruses, but also requires antibacterial activity against various bacteria and antiviral activity against various viruses.

In addition, the antibacterial spectrum of the antibacterial agent in Patent Document 4 is insufficient, and it is required to have antibacterial activity against various bacteria.

Here, in order to obtain a composition having antibacterial activity against various bacteria and antiviral activity against various viruses, it is considered to prepare a plurality of compounds having antibacterial activity against specific bacteria or compounds having antiviral activity against specific viruses. However, if these compounds are arbitrarily selected and a plurality of compounds are blended, in many cases the blended compounds will react with each other or the antibacterial activity or antiviral activity of each compound will be hindered, making it difficult to obtain antibacterial activity against various bacteria. and compositions with antiviral activity against various viruses.

The object of the present invention is to provide an antibacterial and antiviral composition that can be produced through simple steps, is easy to handle, and has antibacterial activity against various bacteria and antiviral activity against various viruses, and an aqueous solution containing the composition.

The present inventor et al. conducted in-depth research and found that if document 5 (Journal of Material Chemistry (Journal of Material Chemistry) of the Royal Society of Chemistry) is selected, The specific metal oxide cluster compounds and specific antibacterial agents described in "Chemistry" Vol. 15, 2005, pages 4773-4782) can be produced through simple steps, are easy to handle, and the compounds do not react with each other, and The present invention is accomplished by not hindering the antibacterial activity or antiviral activity of each compound.

That is, according to the present invention, there is provided: (1) an antibacterial and antiviral composition, comprising: VOSO ₄ , K ₁₁ H [(VO) ₃ (SbW ₉ O ₃₃ ) ₂ ] and Na ₉ (SbW ₉ O ₃₃ ) At least one, and polyhexamethylene biguanide or a salt thereof; (2) an aqueous solution containing the antibacterial and antiviral composition as described in (1).

S1~S8, S11~S22, S31~S34: steps

FIG. 1 is a flowchart showing the manufacturing steps of the wet handkerchief according to the first embodiment.

FIG. 2 is a flowchart showing the manufacturing steps of the wet handkerchief according to the second embodiment.

FIG. 3 is a flowchart showing the manufacturing steps of the wet handkerchief according to the third embodiment.

4a and 4b are observation images obtained by a fluorescence microscope in the antiviral activity measurement of Example 3.

Hereinafter, the antibacterial and antiviral composition and aqueous solution of the present invention will be described. The antibacterial and antiviral composition of the present invention includes: at least one of VOSO ₄ , K ₁₁ H [(VO) ₃ (SbW ₉ O ₃₃ ) ₂ ] and Na ₉ (SbW ₉ O ₃₃ ), and polyhexamethylene biguanide (hereinafter sometimes referred to as "PHMB") or a salt thereof, and the aqueous solution of the present invention contains the antibacterial and antiviral composition.

Here, the aqueous solution of the present invention is a mixture of at least one of VOSO ₄ , K ₁₁ H [(VO) ₃ (SbW ₉ O ₃₃ ) ₂ ], and Na ₉ (SbW ₉ O ₃₃ ), and PHMB or its salt, respectively. formulated in proportions.

VOSO ₄ , K ₁₁ H [(VO) ₃ (SbW ₉ O ₃₃ ) ₂ ], and Na ₉ (SbW ₉ O ₃₃ ) are metal oxide groups called polyoxometalates (PM compounds) clusters of compounds. Here, the PM compound is a metal oxide cluster compound having polyacid ions. Each compound belonging to the PM compound is as described in Document 5 (Journal of Material Chemistry), Vol. 15, pp. 4773-4782, As recorded in the Royal Society of Chemistry (2005), they have their own unique biological activities such as antibacterial activity or antiviral activity. Furthermore, polyacid refers to a metal oxide cluster compound containing a transition metal element (W(VI), V(V), etc.), and has a tetrahedron or octahedron in which oxygen atoms are usually 4-coordinated or 6-coordinated. A structure in which a body is a basic unit and the basic unit is bonded to metal atoms or the like via edges or vertices.

In addition, PHMB is a compound represented by formula (1).

Here, in the formula (1), n represents an integer from 2 to 18, and n is preferably 12.

In addition, PHMB is odorless and has low irritation. It is a safe compound used as an industrial antibacterial agent in 30 countries around the world. In addition, since it is non-volatile, it is a compound whose effect is not easily reduced. Furthermore, unlike hypochlorous acid and the like, it does not corrode metal or rubber, so it is a compound that can easily maintain factory equipment in the manufacturing process.

Furthermore, PHMB can be produced by known methods. In addition, PHMB can also be used as a salt with hydrochloric acid, nitric acid, sulfuric acid, acetic acid, etc., but from the viewpoint of easy availability, it is preferably used as a hydrochloride.

In addition, paragraph [0009] of Japanese Patent Application Laid-Open No. 2018-35280 and paragraph [0009] of Japanese Patent Application Laid-Open No. 2016-160245 state that PHMB alone does not show bacterial spores Bactericidal effect. Therefore, it is considered that even if a compound used together with PHMB is arbitrarily selected, it is impossible to predict whether the bactericidal effect on bacterial spores can be obtained. However, in the antibacterial and antiviral composition and aqueous solution of the present invention, by using PHMB and the PM compound simultaneously , also has a bactericidal effect on spores.

In addition, Japanese Patent Laid-Open No. 2018-35280 and Japanese Patent Laid-Open No. 2016-160245 describe that the pH of the composition containing PHMB is 12.5 or more. In addition, Japanese Patent Laid-Open No. 2017-176606 Paragraph [0027] of Japanese Patent Application Publication No. 2018-44077 and paragraph [0014] of Japanese Patent Application Laid-Open No. 2018-44077 describe that it is difficult to obtain the effect when the pH of the composition containing PHMB is not set to the alkaline range. However, Aqueous solution containing the antibacterial and antiviral composition of the present invention By using PHMB and the PM compound at the same time, the liquid exerts its effect independent of pH as long as it is not extremely acidic or extremely alkaline.

The compounding ratio of the compounds used in the present invention is not particularly limited, but VOSO ₄ is preferably 0.1 mole to 20 mole per mole of K ₁₁ H [(VO) ₃ (SbW ₉ O ₃₃ ) ₂ ]. More preferably, it is 4 moles to 8 moles. Na ₉ (SbW ₉ O ₃₃ ) is preferably 0.1 moles to 30 moles. More preferably, it is 10 moles to 20 moles. PHMB is preferably 0.1 moles to 30 moles. Moles, preferably 1 mole to 5 moles.

In addition, it is particularly preferable to use VOSO ₄ , K ₁₁ H [(VO) ₃ (SbW ₉ O ₃₃ ) ₂ ], and Na ₉ (SbW ₉ O ₃₃ ) in a molar ratio of 5.5:1:17.3:2.3. and PHMB.

When the antibacterial and antiviral composition of the present invention is dissolved in water and used in the form of an aqueous solution, the aqueous solution is prepared so that each compound constituting the antibacterial and antiviral composition reaches a minimum effective concentration or higher. Regarding the concentration of each compound in the aqueous solution of the present invention, VOSO ₄ , K ₁₁ H [(VO) ₃ (SbW ₉ O ₃₃ ) ₂ ], Na ₉ (SbW ₉ O ₃₃ ) and PHMB are preferably 1 μg/mL to 50 μg respectively. /mL, 10μg/mL~300μg/mL, 50μg/mL~1500μg/mL, 1μg/mL~100μg/mL, preferably 20μg/mL~40μg/mL, 50μg/mL~150μg/mL, 500μg/mL~ 1300μg/mL, 5μg/mL~15μg/mL. In addition, regarding the concentration of each compound constituting the antibacterial and antiviral composition in the aqueous solution of the present invention, VOSO ₄ , K ₁₁ H [(VO) ₃ (SbW ₉ O ₃₃ ) ₂ ], Na ₉ (SbW ₉ O ₃₃ ) and The optimal PHMB levels are 25 μg/mL, 115 μg/mL, 1000 μg/mL, and 10 μg/mL respectively.

In addition, the pH of the aqueous solution of the present invention is not particularly limited, but is preferably 3 to 10, and more preferably 4 to 9.

Here, in molar ratio, VOSO ₄ , K ₁₁ H [(VO) ₃ (SbW ₉ O ₃₃ ) ₂ ], Na ₉ (SbW ₉ O ₃₃ ), and PHMB are 5.5:1:17.3:2.3. The minimum inhibitory concentration (MIC) of the prepared aqueous solution containing the antibacterial and antiviral composition of the present invention is shown in Table 1.

As shown in Table 1, even when the concentration of the antibacterial and antiviral composition contained in the aqueous solution of the present invention is low, the growth of microorganisms such as bacteria, fungi (molds), and viruses can be prevented. In addition, the antibacterial composition of the present invention Antiviral compositions and aqueous solutions have a broad antibacterial spectrum. That is, the antibacterial and antiviral composition and aqueous solution of the present invention are active against a wide range of bacterial species. Furthermore, the antibacterial and antiviral composition and aqueous solution of the present invention are also active against Methicillin-resistant Staphylococcus aureus (MRSA). In addition, the aqueous solution containing the antibacterial and antiviral composition of the present invention is stable as an aqueous solution and can be stored for a long time.

In addition, the antibacterial and antiviral composition of the present invention is resistant to high temperatures up to 200°C. It can also maintain antibacterial and antiviral activity and is relatively stable even at high temperatures. In addition, among the compounds constituting the antibacterial and antiviral composition of the present invention, the compounds do not react with each other, and the antibacterial activity or antiviral activity of each compound is not hindered. In addition, these are highly safe compounds that do not cause skin roughness or the like in the human body. Furthermore, the antibacterial and antiviral composition of the present invention is a highly safe compound that does not accumulate in the human body.

In addition, the compounds contained in the antibacterial and antiviral composition and aqueous solution of the present invention are not subject to environmental restrictions. In addition, the manufacturing steps are not complicated, and the cost incurred in manufacturing can be suppressed. In addition, even if dirt adheres to a towel or the like that is the base material of a wet handkerchief described later, the effect of the antibacterial and antiviral composition and aqueous solution of the present invention is not easily reduced. Furthermore, since it is non-volatile, the effect is not easily reduced.

The antibacterial and antiviral composition and aqueous solution of the present invention may also contain other pharmaceutical agents, moisturizers, spices and other ingredients. Examples of humectants include fulvic acid, hyaluronic acid, royal jelly, glycerin, soybean extract, and the like. The fragrance is not particularly limited, and examples thereof include citrus, peppermint, lavender, Lindera umbellata, Magnolia salicifolia Maxim, Chamaecyparis obtusa, and Cryptomeria japonica. ), Japanese fir (Abies firma) and other fragrance ingredients with fragrance, and aroma oils with these fragrances can be used. The antibacterial and antiviral activities and antifungal properties of the antibacterial and antiviral composition and aqueous solution of the present invention are not hindered by other medicinal solutions or moisturizers.

In addition, the antibacterial and antiviral composition of the present invention or water containing the composition The solution may be contained in known wet handkerchiefs such as paper wet handkerchiefs, nonwoven wet handkerchiefs, and cloth wet handkerchiefs, or may be contained in cosmetics such as lotions and lotions. In addition, it can be included in soaps such as hand soap, and can also be included in hygiene products such as masks and cotton swabs. Furthermore, it may also be included in household detergents, kitchen detergents, clothing detergents, etc. used in toilets, bathtubs, kitchens, tableware, clothes, etc. In addition, the antibacterial and antiviral composition of the present invention can be included in the exhaust filter of a car, and the antibacterial and antiviral composition of the present invention or an aqueous solution containing the composition can also be included in the filter of an air conditioner.

In addition, the aqueous solution containing the antibacterial and antiviral composition of the present invention can also be filled into a spray container, and used as a floor or wall of a house, or a table installed in a house, etc., which contains ceramic tiles, metal, plastic, glass, wood, etc. Residential antibacterial and antiviral agents used on fixtures and furniture in houses. In addition, the aqueous solution containing the antibacterial and antiviral composition of the present invention can also be filled into a spray container and used as an antibacterial and antiviral agent for kitchens and antibacterial and antiviral agents for clothing used in toilets, bathtubs, kitchens, clothes, etc. In addition, the antibacterial and antiviral composition of the present invention or an aqueous solution containing the composition can also be used as an antifungal agent. Furthermore, as mentioned above, the antibacterial and antiviral compositions and aqueous solutions of the present invention do not hinder the functions of other pharmaceuticals and other ingredients. In addition, the antibacterial and antiviral activities of the antibacterial and antiviral compositions and aqueous solutions of the present invention are not affected by other pharmaceuticals, etc. Therefore, the antibacterial and antiviral composition and aqueous solution of the present invention may also contain other pharmaceutical agents.

Next, the application of the antibacterial and antiviral composition and aqueous solution of the present invention to wet handkerchiefs will be described. By combining the antibacterial and antiviral composition of the present invention and the water-soluble When the liquid is applied to a wet handkerchief, for example, the antibacterial and antiviral composition or aqueous solution of the present invention can be contained in the base material of the wet handkerchief. Here, as the base material of the wet handkerchief, towels, non-woven fabrics, paper, etc. can be used. As a raw material of towels, cotton etc. are used. In addition, by containing a predetermined amount or more of moisture in the base material, a wet handkerchief can be made.

Hereinafter, a method for manufacturing a wet handkerchief according to the first embodiment of the present invention will be described. In the wet handkerchief of this embodiment, a towel is mainly used as the base material. In addition, the wet handkerchief of this embodiment is a so-called rental wet handkerchief that is used at a rental place and used towels are collected. In addition, the washing of the wet handkerchief in this embodiment is performed in a manner that satisfies the "Guidance Standards Regarding Hygienic Handling of Wet Handkerchiefs, etc." of the Ministry of Health, Labor and Welfare.

FIG. 1 is a flowchart showing the manufacturing steps of the wet handkerchief according to the first embodiment. Towels collected after use in restaurants, etc. are put into the washing tank of the washing machine, detergent is added to perform actual washing (step S1), and low-speed dehydration is performed for a predetermined time (step S2). Here, the actual washing is performed at a predetermined temperature of 60° C. or above and at a medium water level for a predetermined time of 10 minutes or more.

Next, clean water is used to set the washing tank to a high water level, the first rinse is performed at a predetermined temperature for a predetermined time (step S3), and low-speed dehydration is performed for a predetermined time (step S4). Furthermore, clean water is used to set the cleaning tank to a high water level, and bleaching powder or sodium hypochlorite is added so that free chlorine reaches 250 ppm. The second rinse is performed at a prescribed temperature for a prescribed time (step S5), and low-speed dehydration is performed for a prescribed time. (Step S6).

Afterwards, use clean water to set the cleaning tank to the middle water level, and add this The antibacterial and antiviral composition or aqueous solution of the invention is rinsed for the third time at a specified temperature for a specified time (step S7).

When the third rinse is completed, high-speed dehydration is performed for a predetermined time (step S8). Thereby, a prescribed amount of moisture can be included in the towel, and further the antibacterial and antiviral composition or aqueous solution of the present invention can be included in the towel. In addition, a wet handkerchief can be made by adding the antibacterial and antiviral composition or aqueous solution of the present invention to the towel and then giving the towel a predetermined shape. Here, the towel can be given a prescribed shape by folding the towel or rolling the towel. In addition, after being formed into a predetermined shape, it may be packaged with a film or the like.

The wastewater containing the antibacterial and antiviral composition or aqueous solution of the present invention discharged from the cleaning tank is collected into a treatment tank that performs wastewater treatment by a microbial treatment method.

According to this first embodiment, a wet handkerchief having antibacterial activity against various bacteria and antiviral activity against various viruses can be produced. In addition, since an aqueous solution is used instead of an organic solvent, and the antibacterial and antiviral composition or aqueous solution of the present invention is used, it is possible to prevent effects such as rough skin on the human body.

In addition, a composition having excellent antibacterial activity and excellent antiviral activity can be included in the wet handkerchief at low cost. Furthermore, the antibacterial and antiviral composition or aqueous solution of the present invention also has an antifungal effect, and the composition with an antifungal effect can be included in a wet handkerchief at low cost. In addition, drainage containing the antibacterial and antiviral composition or aqueous solution of the present invention will not have any adverse effects such as extinction of microorganisms (bacteria) in the treatment tank where the microorganism treatment method is performed.

Furthermore, in the method of manufacturing a wet handkerchief according to the first embodiment, In the third rinse, the antibacterial and antiviral composition or aqueous solution of the present invention is included in the towel. However, as long as the antibacterial and antiviral composition or aqueous solution of the present invention is included in the towel, it can be done in any step. The towel is made to contain the antibacterial and antiviral composition or aqueous solution of the present invention. For example, the antibacterial and antiviral composition or aqueous solution of the present invention can also be included in the towel during the first rinse or the second rinse. After high-speed dehydration, the towel can be processed as shown in step S21 of Figure 2 below. Since the towel is immersed in the towel dipping tank into which the antibacterial and antiviral composition or aqueous solution of the present invention is added, the towel is immersed in the antibacterial and antiviral composition or aqueous solution of the present invention. Alternatively, the towel may be provided with a predetermined shape and then be sprayed with the antibacterial and antiviral composition or aqueous solution of the present invention to contain the antibacterial and antiviral composition of the present invention.

Next, a method for manufacturing a wet handkerchief according to the second embodiment will be described. Furthermore, in the method of manufacturing a wet handkerchief according to the second embodiment, a new towel is used instead of the towel used as a base material and recycled in the first embodiment. Therefore, detailed description of the same configuration as the first embodiment will be omitted, and only different parts will be described in detail.

Put the new towel into the washing tank, and perform pre-washing for several minutes at a prescribed temperature and a prescribed water level without rotating the drum (step S11). Next, a desizing agent and a scouring penetrant are put in, and formal washing is performed for a prescribed time of more than 10 minutes at a prescribed temperature such as 80°C and a prescribed water level (step S12), and then washed at a prescribed temperature such as 60°C. Cooling is performed for a predetermined time (step S13).

Next, perform rinsing and low-speed dehydration shown in steps S14 to S17. These processes are respectively the same as steps S3 to S6 shown in the flow chart of Figure 1. The principle is the same, so the explanation is omitted.

Thereafter, clean water was used to set the cleaning tank to a predetermined water level, and Nikkanon (manufactured by Nikka Chemical Co., Ltd.) was added so that it became 1 g/L, and the third cycle was performed at a predetermined temperature for a predetermined time. Rinse twice (step S18), and perform high-speed dehydration for a prescribed time (step S19).

Next, the towel that has completed high-speed dehydration is taken out from the cleaning tank and dried at 80° C. for a predetermined time using a dryer (step 20). Thereafter, the towel is made to contain the antibacterial and antiviral composition of the present invention (step S21). Here, the method of containing the antibacterial and antiviral composition of the present invention is not particularly limited. Examples include a method of dipping a towel in an aqueous solution in which the antibacterial and antiviral composition of the present invention is dissolved, or a method of immersing a towel in an aqueous solution in which the antibacterial and antiviral composition of the present invention is dissolved. A method of spraying an aqueous solution of an antiviral composition onto a towel.

In the case of immersion, for example, clean water and the antibacterial and antiviral composition of the present invention are added to a towel immersing tank with a stirring function and stirred to obtain an aqueous solution in which the antibacterial and antiviral composition of the present invention is dissolved, and then the self-cleaning The towels taken out of the tank are immersed in the towel dipping tank. At this time, the concentration of each compound constituting the antibacterial and antiviral composition of the present invention in the aqueous solution is preferably several μg/mL or more. Alternatively, a crane equipped with a towel dipping tool can be used for dipping. In addition, buoyancy can also be added to the towel to prevent the hammer from being immersed in the aqueous solution. Secondly, after the aqueous solution is fully penetrated into the towel in the towel dipping tank, the towel is taken out from the towel dipping tank, lifted and placed until the aqueous solution drips from the towel. Thereafter, the towel is pressed using a pressing machine for several minutes.

Secondly, the towel containing the antibacterial and antiviral composition of the present invention is given The wet handkerchief of this embodiment can be manufactured by packaging the towel in a predetermined shape with a film using a packaging machine (step S22). Furthermore, it is preferable that the water vapor transmission rate of the membrane used in this embodiment is a predetermined value or less.

The wastewater containing the antibacterial and antiviral composition of the present invention and the like discharged from the cleaning tank is collected into a treatment tank that performs wastewater treatment by a microbial treatment method.

According to the method of manufacturing a wet handkerchief of this second embodiment, a wet handkerchief having antibacterial activity against various bacteria and antiviral activity against various viruses can be produced. In addition, since an aqueous solution is used instead of an organic solvent, and the antibacterial and antiviral composition or aqueous solution of the present invention is used, it is possible to prevent effects such as rough skin on the human body.

In addition, a composition having excellent antibacterial activity and excellent antiviral activity can be included in the wet handkerchief at low cost. Furthermore, the antibacterial and antiviral composition and aqueous solution of the present invention also have an antifungal effect, and the composition with an antifungal effect can be included in a wet handkerchief at low cost. In addition, drainage containing the antibacterial and antiviral composition or aqueous solution of the present invention will not have any adverse effects such as extinction of microorganisms (bacteria) in the treatment tank where the microorganism treatment method is performed.

In addition, there is no particular limitation on the washing machine used in the first and second embodiments. You can use a washing machine with one washing tank, you can also use a batch washing machine that performs pre-washing, main washing, rinsing, etc. in separate tanks, or you can use multiple washing machines for pre-wash, main washing, rinsing, etc. in parallel. A continuous washing machine that washes items such as towels while moving them between the bathtubs.

In addition, the wet handkerchief in the first and second embodiments is The manufacturing steps are just an example and can be appropriately changed according to the type and color of the base material.

Next, a method for manufacturing a wet handkerchief according to the third embodiment will be described. Furthermore, in the method of manufacturing a wet handkerchief according to the third embodiment, a structure is changed to use paper, nonwoven fabric, or the like instead of the towel used as the base material in the first embodiment.

First, a roll-shaped base material including paper or nonwoven fabric and a packaging film are placed in a wet handkerchief making machine (step S31). Next, the base material is folded into a predetermined size using a wet handkerchief making machine, and an aqueous solution containing the antibacterial and antiviral composition of the present invention is sprayed from the top and bottom of the folded base material (step S32). Then, the base material is cut into a predetermined size with a rotary knife included in the wet handkerchief making machine (step S33), and the cut base material is packaged with a film (step S34), whereby the wet handkerchief of this embodiment can be produced. .

According to this third embodiment, a wet handkerchief having antibacterial activity against various bacteria and antiviral activity against various viruses can be produced. In addition, since an aqueous solution is used instead of an organic solvent, and an aqueous solution containing the antibacterial and antiviral composition of the present invention is used, effects such as rough skin on the human body can be prevented. In addition, a composition having excellent antibacterial activity and excellent antiviral activity can be included in the wet handkerchief at low cost.

In addition, in the third embodiment, paper or nonwoven fabric is used as the base material, so it is possible to produce a disposable wet handkerchief with excellent antibacterial activity and excellent antiviral activity. Furthermore, the antibacterial and antiviral composition of the present invention also has an antifungal effect, and the composition with an antifungal effect can be included in a wet handkerchief at low cost.

In addition, the wet handkerchief manufactured by the manufacturing method of the third embodiment Wet handkerchiefs meet the hygiene standards of paper wet handkerchiefs set by the Japan Clean Paper and Cotton Industries Association.

Furthermore, as the wet handkerchief produced by this third embodiment, the base materials cut into a predetermined size in step S33 may be packaged individually one by one, or the base materials cut into a predetermined size in step S33 may be packaged individually. The substrate is stored in a package such as a plurality of bottles, and is formed into a so-called wet tissue that can be taken out one by one from the package.

In the case of adopting a structure that can be taken out one by one, it is preferable to give the base material a shape by a folding method or a winding method suitable for taking out one by one from an outlet provided in the package.

In addition, in each of the embodiments described above, the wet handkerchief is configured to contain the antibacterial and antiviral composition of the present invention or an aqueous solution containing the composition. However, the wet handkerchief may also contain other pharmaceutical ingredients. In this case, for example, in the third embodiment, other wet handkerchief solution or moisturizing agent is further added to the aqueous solution containing the antibacterial and antiviral composition of the present invention and sprayed. Examples of humectants include fulvic acid, hyaluronic acid, royal jelly, glycerin, soybean extract, and the like. The antibacterial and antiviral composition or aqueous solution of the present invention does not hinder the functions of other wet handkerchief solutions or moisturizers. In addition, the antibacterial and antiviral activities and antifungal properties of the antibacterial and antiviral composition or aqueous solution of the present invention are not affected by other wet handkerchiefs. Obstacles such as handkerchief solution or moisturizer.

In addition, when the antibacterial and antiviral composition and aqueous solution of the present invention are applied to cosmetics, the antibacterial and antiviral composition or aqueous solution can be mixed and stirred with water, alcohols, oils, moisturizers, whitening agents, and ultraviolet protection agents. Agents, anti-wrinkle agents, stripping agents, fragrances, colorants, surfactants, chelating agents, antioxidants, thickeners, Cosmetics are manufactured from arbitrary components selected from cosmetic raw materials such as pH adjusters.

[Example]

Hereinafter, the present invention will be described in detail using examples. However, the present invention is not limited to the following examples, and can be implemented with any modifications within the scope that does not deviate from the spirit and scope of the present invention.

(Example 1: Determination of antibacterial activity against MRSA)

The aqueous solution containing the antibacterial and antiviral composition of the present invention (VOSO ₄ : 25 μg/mL, K ₁₁ H [(VO) ₃ (SbW ₉ O ₃₃ ) ₂ ] is diluted with 1/20 concentration of Nutrient Broth: 115 μg/mL, Na ₉ (SbW ₉ O ₃₃ ): 1000 μg/mL, PHMB: 10 μg/mL), and 0.1 mL of the test bacterial liquid was added to 10 mL of the specimen dilution solution (5000-fold dilution of the specimen) to prepare Test fluid. This test solution was inoculated into a standard cloth (cotton) for antibacterial testing of a predetermined size (Fiber Evaluation Technology Council), and the test was started.

Here, the test bacteria liquid was used as follows: the test bacteria (Staphylococcus aureus, IID 1677 (MRSA)) were cultured in Nutrient Agar (Difco) at 37°C for 16 to 20 hours, and then allowed to float. It is prepared from nutrient gravy (Difco) with a concentration of 1/20 so that the bacterial count becomes 10 ⁶ /mL~10 ⁷ /mL.

The test solution was stored at 37°C, and the test solution at the beginning (immediately after inoculation) and 18 hours later was immediately diluted to 10 times on the culture medium for bacterial count determination, and the viable bacterial count in the test solution was used for measurement. In addition, the culture medium for bacterial count measurement was based on the mixed plate culture method, and SCDLP agar medium (manufactured by Nippon Pharmaceutical Co., Ltd.) was cultured at 35°C ± 1°C for 2 days.

In addition, as a control, 0.1 mL of the test bacterial solution was added to nutrient gravy with a concentration of 1/20, and inoculated into a standard cloth (cotton) for antibacterial testing of the same size as the above (General Incorporated Fiber Evaluation Technology Council) ), conduct the same test. The number of viable bacteria at the beginning (immediately after inoculation) and 18 hours later was measured. The results are shown in Table 2. Furthermore, the aqueous solution containing the antibacterial and antiviral composition of the present invention and the control were measured three times each.

Bacterial liquid preparation solution: 1/20 concentration nutrient medium

Control: Standard cloth (cotton) [Fiber Evaluation Technology Council]

<20: Not detected

As shown in Table 2, compared with the case where the aqueous solution containing the antibacterial and antiviral composition of the present invention is not used, the number of viable bacteria of MRSA can be suppressed when using the aqueous solution containing the antibacterial and antiviral composition of the present invention. The anti-MRSA effect of the antibacterial and antiviral composition and aqueous solution of the present invention is demonstrated.

(Example 2: Determination of antibacterial activity against Bacillus cereus)

The aqueous solution containing the antibacterial and antiviral composition of the present invention (VOSO ₄ : 25 μg/mL, K ₁₁ H [(VO) ₃ (SbW ₉ O ₃₃ ) ₂ ]: 115 μg/mL, Na ₉ (SbW ₉ O ₃₃ ): 1000 μg/mL, PHMB: 10 μg/mL), add Bacillus cactus to 10 mL of sample dilution (5000-fold dilution of the sample), and inoculate it into a standard cloth for antibacterial testing of a specified size. (Cotton) (General incorporated fiber evaluation technology council). As the cactus bacterium, one strain of cactus bacillus IFO13494 (cactus bacilli) was used. Furthermore, this measurement is based on the 10.1 bacterial liquid absorption method of the 10 quantitative test in Japanese Industrial Standards (JIS) L 1902:2008 "Antibacterial Test Methods and Antibacterial Effects of Fiber Products" to perform antibacterial testing of the specimen. force test. In addition, in this antibacterial power test, high-pressure steam sterilization (121° C., 15 minutes) of the specimen was not performed.

In addition, as a control, one strain of the cactus bacterium IFO13494 (cactus bacilli) was added to nutrient gravy with a concentration of 1/20, and was inoculated into a standard cloth (cotton) for antibacterial testing (cotton) of the same size as the above. Fiber Evaluation Technology Council), conducted the same test. The number of viable bacteria at the beginning (immediately after inoculation) and 18 hours later was measured. The results are shown in Table 3.

In addition, a test using Bacillus opuntia spores (Bacillus opuntia IFO 13494 (Bacillus opuntia, spores)) instead of Bacillus opuntia was conducted in the same manner, and a control test was conducted similarly. The number of viable bacteria in the test piece was measured immediately after inoculation of Cactus spores and after 18 hours of culture, and is shown in Table 3.

Bacterial liquid preparation solution: 1/20 concentration nutrient medium

Control: Standard cloth (cotton) [Fiber Evaluation Technology Council]

<20: Not detected

According to the results shown in Table 3, compared with the case where the aqueous solution containing the antibacterial and antiviral composition of the present invention is not used, when the aqueous solution containing the antibacterial and antiviral composition of the present invention is used, it is possible to inhibit the growth of Opuntia cactus and Opuntia cactus. The viable bacterial count of Bacillus spores shows the antibacterial and antiviral composition and aqueous solution of the present invention have the anti-cactus bacterium effect.

(Example 3: Determination of antiviral activity against influenza virus)

The aqueous solution containing the antibacterial and antiviral composition of the present invention (VOSO ₄ : 25 μg/mL, K ₁₁ H [(VO) ₃ (SbW ₉ O ₃₃ ) ₂ ]: 115 μg/mL, Na ₉ (SbW ₉ O ₃₃ ): 1000 μg/mL, PHMB: 10 μg/mL), and a standard cloth (cotton) for antibacterial testing of a specified size is added to the sample dilution (5000-fold dilution of the sample) (General Society Corporate Fiber Evaluation Technology Council) and obtained test specimens. Next, the test specimen was immersed in an influenza virus solution at 37° C. for 5 minutes. Then, the test specimen is taken out from the influenza virus solution and immersed in the antiviral solution. After removing the test specimen from the antiviral solution, it is processed with fluorescently labeled virus antibodies and observed with a fluorescence microscope. The observation results obtained by fluorescence microscopy are shown in Figure 4a. In addition, a control experiment was conducted in the same procedure except that water was used instead of the aqueous solution containing the antibacterial and antiviral composition of the present invention. The observation results obtained by fluorescence microscopy of the control experiment are shown in Figure 4b. In addition, in Figures 4a and 4b, the unblackened portion is an area where fluorescence is observed, indicating that influenza virus is present in the area where fluorescence is observed.

According to the results shown in Figure 4a and Figure 4b, compared with the case where the aqueous solution containing the antibacterial and antiviral composition of the present invention is not used, almost no difference is observed in the case of using the aqueous solution containing the antibacterial and antiviral composition of the present invention. The anti-influenza virus effect of the antibacterial and antiviral composition and aqueous solution of the present invention is demonstrated against influenza virus.

S1~S8: steps

Claims (14)

An antibacterial and antiviral composition, comprising: at least one of VOSO ₄ , K ₁₁ H [(VO) ₃ (SbW ₉ O ₃₃ ) ₂ ], and Na ₉ (SbW ₉ O ₃₃ ); and polyhexamethylene biguanide or Its salt. The antibacterial and antiviral composition described in item 1 of the patent application has a bactericidal effect on bacterial spores. An aqueous solution containing the antibacterial and antiviral composition as described in item 1 or 2 of the patent application. A kind of soap, including the antibacterial and antiviral composition as described in item 1 or 2 of the patent application scope. A sanitary product including an antibacterial and antiviral composition as described in item 1 or 2 of the patent application scope. A detergent for residential use, including an antibacterial and antiviral composition as described in Item 1 or Item 2 of the patent application. A kitchen detergent, including the antibacterial and antiviral composition described in item 1 or 2 of the patent application scope. A detergent for clothing, including the antibacterial and antiviral composition described in item 1 or 2 of the patent application scope. An antibacterial and antiviral agent for residential use, including the antibacterial and antiviral composition described in item 1 or 2 of the patent application scope. An antibacterial and antiviral agent for kitchen use, including No. 1 of the patent application The antibacterial and antiviral composition described in item 2 or item 2. An antibacterial and antiviral agent for clothing materials, including the antibacterial and antiviral composition described in item 1 or 2 of the patent application scope. A cosmetic product including an antibacterial and antiviral composition as described in item 1 or item 2 of the patent application scope. A kind of wet wipes, including the antibacterial and antiviral composition as described in item 1 or 2 of the patent application scope. A wet handkerchief includes the antibacterial and antiviral composition described in item 1 or 2 of the patent application scope.

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