CN115025615B - Multi-effect active biological enzyme and preparation method thereof - Google Patents
Multi-effect active biological enzyme and preparation method thereof Download PDFInfo
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- CN115025615B CN115025615B CN202110804908.8A CN202110804908A CN115025615B CN 115025615 B CN115025615 B CN 115025615B CN 202110804908 A CN202110804908 A CN 202110804908A CN 115025615 B CN115025615 B CN 115025615B
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- 102000004190 Enzymes Human genes 0.000 title claims abstract description 104
- 108090000790 Enzymes Proteins 0.000 title claims abstract description 104
- 238000002360 preparation method Methods 0.000 title abstract description 7
- 239000000126 substance Substances 0.000 claims abstract description 10
- 229940088598 enzyme Drugs 0.000 claims description 100
- RWSXRVCMGQZWBV-WDSKDSINSA-N glutathione Chemical group OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 claims description 58
- 239000004365 Protease Substances 0.000 claims description 56
- 108010024636 Glutathione Proteins 0.000 claims description 31
- 229960003180 glutathione Drugs 0.000 claims description 27
- 108090000526 Papain Proteins 0.000 claims description 20
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 20
- 239000003242 anti bacterial agent Substances 0.000 claims description 20
- 239000003085 diluting agent Substances 0.000 claims description 20
- 229940055729 papain Drugs 0.000 claims description 20
- 235000019834 papain Nutrition 0.000 claims description 20
- 108010004032 Bromelains Proteins 0.000 claims description 18
- 235000019835 bromelain Nutrition 0.000 claims description 18
- 239000002781 deodorant agent Substances 0.000 claims description 17
- 102000004142 Trypsin Human genes 0.000 claims description 15
- 108090000631 Trypsin Proteins 0.000 claims description 15
- 239000012588 trypsin Substances 0.000 claims description 15
- 229920002527 Glycogen Polymers 0.000 claims description 13
- 229940096919 glycogen Drugs 0.000 claims description 13
- 229920000642 polymer Polymers 0.000 claims description 12
- 239000002994 raw material Substances 0.000 claims description 12
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 claims description 10
- 150000004676 glycans Chemical class 0.000 claims description 7
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical group C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 claims description 7
- 229920001282 polysaccharide Polymers 0.000 claims description 7
- 239000005017 polysaccharide Substances 0.000 claims description 7
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 7
- 229920001184 polypeptide Polymers 0.000 claims description 6
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 229920000869 Homopolysaccharide Polymers 0.000 claims description 5
- 238000000227 grinding Methods 0.000 claims description 5
- 238000002156 mixing Methods 0.000 claims description 5
- 229920002101 Chitin Polymers 0.000 claims description 2
- 229920002472 Starch Polymers 0.000 claims description 2
- 229920002678 cellulose Polymers 0.000 claims description 2
- 239000001913 cellulose Substances 0.000 claims description 2
- 239000008107 starch Substances 0.000 claims description 2
- 235000019698 starch Nutrition 0.000 claims description 2
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 abstract description 66
- 230000000694 effects Effects 0.000 abstract description 17
- 238000005507 spraying Methods 0.000 abstract description 6
- 239000012466 permeate Substances 0.000 abstract description 3
- 230000002045 lasting effect Effects 0.000 abstract description 2
- 230000001699 photocatalysis Effects 0.000 abstract description 2
- 238000007146 photocatalysis Methods 0.000 abstract description 2
- 238000000746 purification Methods 0.000 abstract 1
- 102000035195 Peptidases Human genes 0.000 description 18
- 108091005804 Peptidases Proteins 0.000 description 18
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 18
- 235000019419 proteases Nutrition 0.000 description 17
- 239000000047 product Substances 0.000 description 12
- 238000000034 method Methods 0.000 description 11
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical class [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 8
- 102000005600 Cathepsins Human genes 0.000 description 6
- 108010084457 Cathepsins Proteins 0.000 description 6
- 102000012479 Serine Proteases Human genes 0.000 description 5
- 108010022999 Serine Proteases Proteins 0.000 description 5
- 239000007789 gas Substances 0.000 description 5
- 229920000587 hyperbranched polymer Polymers 0.000 description 5
- 239000002086 nanomaterial Substances 0.000 description 5
- 241000196324 Embryophyta Species 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 230000007613 environmental effect Effects 0.000 description 4
- 235000013373 food additive Nutrition 0.000 description 4
- 239000002778 food additive Substances 0.000 description 4
- 238000003756 stirring Methods 0.000 description 4
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 3
- 229910021389 graphene Inorganic materials 0.000 description 3
- 229940081969 saccharomyces cerevisiae Drugs 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- 101710097834 Thiol protease Proteins 0.000 description 2
- XLOMVQKBTHCTTD-UHFFFAOYSA-N Zinc monoxide Chemical compound [Zn]=O XLOMVQKBTHCTTD-UHFFFAOYSA-N 0.000 description 2
- 230000003213 activating effect Effects 0.000 description 2
- 230000004913 activation Effects 0.000 description 2
- 239000004480 active ingredient Substances 0.000 description 2
- 239000004599 antimicrobial Substances 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000002184 metal Chemical class 0.000 description 2
- 229910052751 metal Chemical class 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- 239000011941 photocatalyst Substances 0.000 description 2
- 230000002035 prolonged effect Effects 0.000 description 2
- 239000012629 purifying agent Substances 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 102100039702 Alcohol dehydrogenase class-3 Human genes 0.000 description 1
- 241000222120 Candida <Saccharomycetales> Species 0.000 description 1
- 102000030523 Catechol oxidase Human genes 0.000 description 1
- 108010031396 Catechol oxidase Proteins 0.000 description 1
- 108010035532 Collagen Proteins 0.000 description 1
- 102000008186 Collagen Human genes 0.000 description 1
- QPLDLSVMHZLSFG-UHFFFAOYSA-N Copper oxide Chemical compound [Cu]=O QPLDLSVMHZLSFG-UHFFFAOYSA-N 0.000 description 1
- 239000005751 Copper oxide Substances 0.000 description 1
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 1
- MVORZMQFXBLMHM-QWRGUYRKSA-N Gly-His-Lys Chemical compound NCCCC[C@@H](C(O)=O)NC(=O)[C@@H](NC(=O)CN)CC1=CN=CN1 MVORZMQFXBLMHM-QWRGUYRKSA-N 0.000 description 1
- 241000235058 Komagataella pastoris Species 0.000 description 1
- GDBQQVLCIARPGH-UHFFFAOYSA-N Leupeptin Natural products CC(C)CC(NC(C)=O)C(=O)NC(CC(C)C)C(=O)NC(C=O)CCCN=C(N)N GDBQQVLCIARPGH-UHFFFAOYSA-N 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 1
- 102000019197 Superoxide Dismutase Human genes 0.000 description 1
- 108010012715 Superoxide dismutase Proteins 0.000 description 1
- 229930003268 Vitamin C Natural products 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 239000012190 activator Substances 0.000 description 1
- 125000003172 aldehyde group Chemical group 0.000 description 1
- -1 amino acid compounds Chemical class 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 125000003277 amino group Chemical group 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 239000012752 auxiliary agent Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 229920001436 collagen Polymers 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 229910000431 copper oxide Inorganic materials 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 230000007797 corrosion Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000001877 deodorizing effect Effects 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- 108010051015 glutathione-independent formaldehyde dehydrogenase Proteins 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 229940059442 hemicellulase Drugs 0.000 description 1
- 108010002430 hemicellulase Proteins 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 239000010985 leather Substances 0.000 description 1
- 108010052968 leupeptin Proteins 0.000 description 1
- GDBQQVLCIARPGH-ULQDDVLXSA-N leupeptin Chemical compound CC(C)C[C@H](NC(C)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@H](C=O)CCCN=C(N)N GDBQQVLCIARPGH-ULQDDVLXSA-N 0.000 description 1
- 229920005610 lignin Polymers 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 229910044991 metal oxide Inorganic materials 0.000 description 1
- 150000004706 metal oxides Chemical class 0.000 description 1
- WSFSSNUMVMOOMR-NJFSPNSNSA-N methanone Chemical compound O=[14CH2] WSFSSNUMVMOOMR-NJFSPNSNSA-N 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 210000002345 respiratory system Anatomy 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 150000003573 thiols Chemical class 0.000 description 1
- 235000019154 vitamin C Nutrition 0.000 description 1
- 239000011718 vitamin C Substances 0.000 description 1
- 239000011787 zinc oxide Substances 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/74—General processes for purification of waste gases; Apparatus or devices specially adapted therefor
- B01D53/84—Biological processes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/38—Removing components of undefined structure
- B01D53/44—Organic components
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/46—Removing components of defined structure
- B01D53/54—Nitrogen compounds
- B01D53/58—Ammonia
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/46—Removing components of defined structure
- B01D53/72—Organic compounds not provided for in groups B01D53/48 - B01D53/70, e.g. hydrocarbons
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2257/00—Components to be removed
- B01D2257/70—Organic compounds not provided for in groups B01D2257/00 - B01D2257/602
- B01D2257/702—Hydrocarbons
- B01D2257/7027—Aromatic hydrocarbons
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2257/00—Components to be removed
- B01D2257/70—Organic compounds not provided for in groups B01D2257/00 - B01D2257/602
- B01D2257/708—Volatile organic compounds V.O.C.'s
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2258/00—Sources of waste gases
- B01D2258/06—Polluted air
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/20—Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters
Abstract
The first aspect of the invention provides a multi-effect active biological enzyme, the second aspect of the invention provides a preparation method of the multi-effect active biological enzyme, and the third aspect of the invention provides an application of the multi-effect active biological enzyme. The multifunctional active biological enzyme provided by the invention can play a role in purifying air without photocatalysis, and the biological enzyme is used for spraying wet objects, so that the biological enzyme can permeate into the objects, promote the generation and decompose harmful substances such as formaldehyde and peculiar smell molecules in the bodies, eliminate the harmful substances and peculiar smell from the source, and achieve the effect of lasting purification.
Description
Technical Field
The invention belongs to the fields of B01D53/84 and B01D53/72, and particularly relates to a multifunctional active biological enzyme and a preparation method thereof.
Background
Formaldehyde is a colorless and pungent gas, and has stimulating effect on human eyes, nose, respiratory tract, etc. Products for removing formaldehyde in the market are many, and some products rely on chemical reagents to degrade the formaldehyde by a pure chemical means, but the chemical reagents degrade the formaldehyde to cause secondary pollution easily; some formaldehyde is removed by means of a photocatalyst technology, chinese patent CN110743357A is prepared by compounding a plurality of metal oxides and metal salts to obtain a photocatalyst, and then a biological enzyme auxiliary agent containing high molecular organic substances is combined to prepare formaldehyde-removing liquid, but the formaldehyde-removing liquid prepared by the patent needs to be sprayed and used respectively in the using process, so that the using complexity is increased; the formaldehyde is removed by means of biotechnology, but biological products are easily limited by using conditions in the using process, such as China patent 107174947A, which discloses a formaldehyde deodorizing and purifying agent composed of a plurality of amino acid compounds, inorganic salts and high molecular organic substances, but a plurality of amino acids used in the products prepared by the patent are easy to oxidize and degrade, and long-term removal of formaldehyde cannot be realized. Based on the applicant, a formaldehyde removal product with durable effect and convenient use is provided.
Disclosure of Invention
In a first aspect, the present invention provides a multi-efficiency active biological enzyme, the raw materials comprising: biological enzymes, polysaccharides, polypeptides, yeasts, deodorants.
Preferably, the biological enzyme is at least one selected from formaldehyde dehydrogenase, polyphenol oxidase, superoxide dismutase, hemicellulase, lignin enzyme, protease.
Preferably, the protease is selected from at least one of a plant protease and a microbial protease.
Preferably, the plant protease is at least one selected from bromelain, papain, bean trypsin and corn protease.
Preferably, the plant protease is bromelain or papain.
Preferably, the microbial protease is a cathepsin.
Preferably, the mass ratio of the bromelain, the cathepsin and the papain is 2.5-5:1-3:10-15.
Bromelain is purchased from the biological sciences of Henghua-dak, nanning.
Papain is purchased from western amp Murson bioengineering limited.
Preferably, the cathepsin is trypsin, which is purchased from henry rella biotechnology limited.
Preferably, the polysaccharide is a homopolysaccharide.
Preferably, the homopolysaccharide is at least one selected from cellulose, glycogen, starch, and chitin.
Preferably, the homopolysaccharide is glycogen.
Preferably, the glycogen is purchased from Shanghai Drum Biotechnology Co., ltd., CAS number: 9005-79-2.
Preferably, the polypeptide is a tripeptide.
Preferably, the tripeptide substance is at least one selected from glutathione, collagen tripeptide, copper peptide and leupeptin.
Preferably, the tripeptide is glutathione.
Preferably, the addition mass of the glutathione is 0.5-2.5% of the mass of the protease.
Glutathione is reduced glutathione, purchased from zhengzhou department of food additives, ltd.
Applicants have found during the course of the assay that the addition of glutathione to proteases can stimulate and increase the activity of biological enzymes. The possible reasons the applicant speculates are that glutathione is just an activator of the bio-enzyme selected in the present invention, activates the active ingredient in the bio-enzyme selected in the present invention, and that glutathione may also bind to free radicals, protecting the active ingredient in the activated bio-enzyme from being destroyed.
However, the applicant found that the addition amount of glutathione affects the activity of the biological enzyme, and that when the addition amount of glutathione exceeds a certain amount, the activation of the biological enzyme by glutathione becomes an activity inhibition, thereby affecting the activity of the biological enzyme.
Although glutathione has an activating effect on biological enzymes, glutathione has different activating capacities on different kinds of biological enzymes, and the applicant finds that when the biological enzymes are selected from cathepsin, bromelain and papain, the biological enzymes show the best activity, and the prepared product has the best speed and effect on formaldehyde removal. The applicant speculates that the possible reasons are that cathepsin, bromelain and papain all belong to thiol protease, that glutathione has excellent activation effect on thiol protein, that the existence of thiol protease can promote the reaction between aldehyde group, amino group and other functional groups, and that the formaldehyde removal effect is improved.
However, the applicant found that changing the mass ratio of cathepsin, bromelain and papain affects the optimal conditions of use of the final product, and also affects the smell of the product.
Preferably, the yeast is at least one selected from pichia pastoris, candida, baker's yeast and saccharomyces cerevisiae.
Preferably, the yeast is Saccharomyces cerevisiae.
Saccharomyces cerevisiae was purchased from Guangdong Eusman Biotechnology Inc.
Preferably, the deodorant is a polymer deodorant.
The macromolecular deodorant is purchased from Shanghai Yi Fu technology Co., ltd, and has the brand name: kalmor.
Preferably, the multifunctional active biological enzyme comprises the following raw materials in parts by weight: 90-110 parts of biological enzyme, 40-80 parts of polysaccharide, 0.45-2.75 parts of glutathione, 5-20 parts of yeast and 80-100 parts of deodorant.
The second aspect of the present invention provides a method for preparing a multi-functional active biological enzyme, comprising the steps of: and uniformly mixing the biological enzyme, the polysaccharide, the polypeptide, the yeast and the deodorant, and grinding to obtain the active biological enzyme.
The third aspect of the invention provides application of the multifunctional active biological enzyme, which is obtained by dissolving the multifunctional active biological enzyme in a diluent, uniformly stirring and spraying.
Preferably, the mass ratio of the multi-effect active biological enzyme to the diluent is 1: (40-80).
Preferably, the raw materials of the diluent comprise amino polymer, antibacterial agent and water.
Preferably, the raw materials of the diluent comprise 0.1-1% of amino polymer, 0.01-1% of antibacterial agent and the balance of water according to mass percent.
Preferably, the antibacterial agent is at least one selected from inorganic antibacterial agents and natural antibacterial agents.
Preferably, the inorganic antibacterial agent is at least one selected from modified graphene, activated carbon, zinc oxide, copper oxide, nano silver and nano gold.
Preferably, the modified graphene is vitamin C reduced graphene oxide.
Vitamin C reduced graphene oxide is purchased from Jiangsu Xianfeng nanomaterials technology Co.
The applicant finds that the vitamin C reduced graphene oxide is used as an antibacterial agent in the test process, so that a good antibacterial effect can be achieved, and the effective duration of formaldehyde removal of the product can be prolonged. The applicant speculates that the possible reasons are that the specific graphene oxide has rich cavity structures, so that the specific protease in the system can be effectively attached to the graphene oxide, the stability of the specific protease is improved, the effective duration of formaldehyde removal is prolonged, and the vitamin C on the surface of the specific graphene oxide can be combined with free radicals to protect the specific protease from being oxidized, and the effective duration of formaldehyde removal of the product is improved by changing the conformation modes such as folding, curling and the like of the secondary peptide chain of the specific protease.
Preferably, the mass ratio of the antibacterial agent to the protease is 1: (10-20).
Applicants have found during the test that when the mass ratio of antimicrobial agent to protease is 1: (10-20), the prepared biological enzyme product can show excellent formaldehyde removal effect, and when the content of the antibacterial agent exceeds 10% of the content of the protease, the protease activity is inhibited, and the formaldehyde removal effect cannot be shown.
Preferably, the amino polymer is an amino-terminated hyperbranched polymer.
In the invention, amino-terminated hyperbranched polymers are purchased from Chengdu century-inherited environmental protection technology Co., ltd (North Sichuan general agency), and have the following marks: north.
Compared with the prior art, the invention has the beneficial effects that:
the multifunctional active biological enzyme provided by the invention can play a role in purifying air without photocatalysis, and the biological enzyme is used for spraying wet objects, so that the biological enzyme can permeate into the objects, promote the generation and decompose harmful substances such as formaldehyde and peculiar smell molecules in the bodies, eliminate the harmful substances and peculiar smell from the source, and achieve a lasting purifying effect. The product can be sprayed on any furniture, metal, plant, leather and other articles, fully permeates and is treated from the source, is not easy to rebound, has no corrosion and oxidation effect, does not cause mildew and oxidation, is nontoxic and harmless, can be rapidly degraded, has no secondary pollution, can be used for efficiently and rapidly purifying air, has long degradation effect, can be applied to various occasions for rapidly treating various harmful gases such as formaldehyde, benzene, ammonia, TVOC and the like, and has long effect.
Detailed Description
Example 1
The first aspect of the invention provides a multi-effect active biological enzyme, which comprises, by weight, 110 parts of biological enzyme, 75 parts of glycogen, 2.5 parts of glutathione, 18 parts of saccharomyces cerevisiae and 100 parts of high molecular deodorant.
The biological enzymes are bromelain, trypsin and papain, and the mass ratio of bromelain, trypsin and papain is 3.5:1.5:10.
among them, trypsin is purchased from henry rella biotechnology limited.
Bromelain is purchased from the biological sciences of Henghua-dak, nanning.
Papain is purchased from western amp Murson bioengineering limited.
Glutathione is reduced glutathione, purchased from zhengzhou department of food additives, ltd.
Glycogen is purchased from Shanghai Drum ministerial Biotechnology Co., ltd., CAS number: 9005-79-2.
Saccharomyces cerevisiae was purchased from Guangdong Eusman Biotechnology Inc.
The macromolecular deodorant is purchased from Shanghai Yi Fu technology Co., ltd, and has the brand name: kalmor.
The second aspect of the present invention provides a method for preparing a multi-functional active biological enzyme, comprising the steps of: and uniformly mixing the biological enzyme, glycogen, glutathione, saccharomyces cerevisiae and a high molecular deodorant, and grinding by a grinder to obtain the active biological enzyme.
The third aspect of the present invention provides an application of a multifunctional active biological enzyme:
(1) Preparing a diluent: adding 1% of amino polymer and 0.09% of antibacterial agent into the balance of water according to mass percent, and uniformly stirring to obtain the diluent;
(2) And dissolving the multi-effect active biological enzyme by using a diluent to obtain a use solution of the multi-effect active biological enzyme.
Wherein the mass ratio of the multi-effect active biological enzyme to the diluent is 1:40.
the amino polymer is an amino-terminated hyperbranched polymer, and is purchased from Chengdu century-inherited environmental protection technology limited company (North Sichuan general agency), and has the brand name: north.
The antibacterial agent is vitamin C reduced graphene oxide purchased from Jiangsu Xianfeng nano materials science and technology Co.
Example 2
The first aspect of the invention provides a multi-effect active biological enzyme, which comprises, by weight, 100 parts of biological enzyme, 50 parts of glycogen, 2 parts of glutathione, 10 parts of saccharomyces cerevisiae and 80 parts of high molecular deodorant.
The biological enzymes are bromelain, trypsin and papain, and the mass ratio of bromelain, trypsin and papain is 3.5:1.5:10.
among them, trypsin is purchased from henry rella biotechnology limited.
Bromelain is purchased from the biological sciences of Henghua-dak, nanning.
Papain is purchased from western amp Murson bioengineering limited.
Glutathione is reduced glutathione, purchased from zhengzhou department of food additives, ltd.
Glycogen is purchased from Shanghai Drum ministerial Biotechnology Co., ltd., CAS number: 9005-79-2.
Saccharomyces cerevisiae was purchased from Guangdong Eusman Biotechnology Inc.
The macromolecular deodorant is purchased from Shanghai Yi Fu technology Co., ltd, and has the brand name: kalmor.
The second aspect of the present invention provides a method for preparing a multi-functional active biological enzyme, comprising the steps of: and uniformly mixing the biological enzyme, glycogen, glutathione, saccharomyces cerevisiae and a high molecular deodorant, and grinding by a grinder to obtain the active biological enzyme.
The third aspect of the present invention provides an application of a multifunctional active biological enzyme:
(1) Preparing a diluent: adding 1% of amino polymer and 0.1% of antibacterial agent into the balance of water according to mass percent, and uniformly stirring to obtain the diluent;
(2) And dissolving the multi-effect active biological enzyme by using a diluent to obtain a use solution of the multi-effect active biological enzyme.
Wherein the mass ratio of the multi-effect active biological enzyme to the diluent is 1:40.
the amino polymer is an amino-terminated hyperbranched polymer, and is purchased from Chengdu century-inherited environmental protection technology limited company (North Sichuan general agency), and has the brand name: north.
The antibacterial agent is vitamin C reduced graphene oxide purchased from Jiangsu Xianfeng nano materials science and technology Co.
Example 3
The first aspect of the invention provides a multi-effect active biological enzyme, which comprises, by weight, 90 parts of biological enzyme, 40 parts of glycogen, 1 part of glutathione, 5 parts of saccharomyces cerevisiae and 80 parts of polymer deodorant.
The biological enzymes are bromelain, trypsin and papain, and the mass ratio of bromelain, trypsin and papain is 3.5:1.5:10.
among them, trypsin is purchased from henry rella biotechnology limited.
Bromelain is purchased from the biological sciences of Henghua-dak, nanning.
Papain is purchased from western amp Murson bioengineering limited.
Glutathione is reduced glutathione, purchased from zhengzhou department of food additives, ltd.
Glycogen is purchased from Shanghai Drum ministerial Biotechnology Co., ltd., CAS number: 9005-79-2.
Saccharomyces cerevisiae was purchased from Guangdong Eusman Biotechnology Inc.
The macromolecular deodorant is purchased from Shanghai Yi Fu technology Co., ltd, and has the brand name: kalmor.
The second aspect of the present invention provides a method for preparing a multi-functional active biological enzyme, comprising the steps of: and uniformly mixing the biological enzyme, glycogen, glutathione, saccharomyces cerevisiae and a high molecular deodorant, and grinding by a grinder to obtain the active biological enzyme.
The third aspect of the present invention provides an application of a multifunctional active biological enzyme:
(1) Preparing a diluent: adding 1% of amino polymer and 0.1% of antibacterial agent into the balance of water according to mass percent, and uniformly stirring to obtain the diluent;
(2) And dissolving the multi-effect active biological enzyme by using a diluent to obtain a use solution of the multi-effect active biological enzyme.
Wherein the mass ratio of the multi-effect active biological enzyme to the diluent is 1:40.
the amino polymer is an amino-terminated hyperbranched polymer, and is purchased from Chengdu century-inherited environmental protection technology limited company (North Sichuan general agency), and has the brand name: north.
The antibacterial agent is vitamin C reduced graphene oxide purchased from Jiangsu Xianfeng nano materials science and technology Co.
Example 4
The first aspect of the invention provides a multi-functional active biological enzyme, the second aspect of the invention provides a preparation method of the multi-functional active biological enzyme, and the third aspect of the invention provides an application of the multi-functional active biological enzyme, and the specific raw materials and embodiments are the same as those of example 1, except that the addition amount of glutathione is 5 parts.
Example 5
The first aspect of the invention provides a multi-functional active biological enzyme, the second aspect of the invention provides a preparation method of the multi-functional active biological enzyme, and the third aspect of the invention provides an application of the multi-functional active biological enzyme, and the specific raw materials and embodiments are the same as those of example 1, except that glutathione is not added.
Example 6
The first aspect of the present invention provides a multi-functional active biological enzyme, the second aspect of the present invention provides a method for preparing the multi-functional active biological enzyme, and the third aspect of the present invention provides an application of the multi-functional active biological enzyme, wherein the raw materials and embodiments are the same as those of example 1, and the difference is that the biological enzyme is serine protease, and the serine protease is purchased from the company of biological engineering of Seiran Hao.
Example 7
The first aspect of the present invention provides a multi-functional active biological enzyme, the second aspect of the present invention provides a method for preparing the multi-functional active biological enzyme, and the third aspect of the present invention provides an application of the multi-functional active biological enzyme, and the specific raw materials and embodiments used are the same as those in example 1, wherein the biological enzyme is serine protease, trypsin, papain, and the mass ratio of serine protease, trypsin, papain is 3.5:1.5:10, said serine protease is purchased from Seaman bioengineering Inc.
Example 8
The first aspect of the present invention provides a multifunctional active biological enzyme, the second aspect of the present invention provides a method for preparing the multifunctional active biological enzyme, and the third aspect of the present invention provides an application of the multifunctional active biological enzyme, wherein the specific raw materials and embodiments used are the same as those of example 1, and the antimicrobial agent is unmodified graphene oxide, purchased from Jiangsu first Feng nano materials science and technology Co., ltd, with the product number of 102740.
Example 9
The first aspect of the invention provides a multi-functional active biological enzyme, the second aspect of the invention provides a preparation method of the multi-functional active biological enzyme, and the third aspect of the invention provides an application of the multi-functional active biological enzyme, and the specific raw materials and embodiments are the same as those of example 1, except that no antibacterial agent is added.
Example 10
The first aspect of the present invention provides a multi-functional active biological enzyme, the second aspect of the present invention provides a method for preparing the multi-functional active biological enzyme, and the third aspect of the present invention provides an application of the multi-functional active biological enzyme, wherein the raw materials and embodiments are the same as those of example 1, and the addition amount of the antibacterial agent is 5%.
Performance testing
And dissolving the active biological enzyme prepared in the embodiment in the diluent prepared in the corresponding embodiment to obtain an active biological enzyme use solution. Spraying the active biological enzyme using solution into a glass box containing formaldehyde gas with a certain concentration, wherein the initial concentration of formaldehyde is marked as C0, the spraying amount of the active biological enzyme using solution is 5ml, collecting the gas in the glass box after the spraying of the using solution for 6 hours, 12 hours, 18 hours, 24 hours and 48 hours respectively, and measuring the concentration of formaldehyde in the gas and marking as C. The removal efficiency of the biological enzyme-containing air purifying agent to formaldehyde is obtained through calculation. The data are recorded in table 1.
TABLE 1
Claims (3)
1. The application of the multifunctional active biological enzyme is characterized in that the multifunctional active biological enzyme is dissolved in a diluent, and is sprayed for use after being uniformly stirred;
the raw materials of the multifunctional active biological enzyme comprise: biological enzymes, polysaccharides, polypeptides, yeast, deodorants; uniformly mixing biological enzyme, polysaccharide, polypeptide, yeast and deodorant, and grinding to obtain the active biological enzyme;
the biological enzyme is bromelain, trypsin and papain; the mass ratio of the bromelain to the trypsin to the papain is 2.5-5:1-3:10-15 parts;
the polypeptide is tripeptide substance; the tripeptide substance is glutathione, and the added mass of the glutathione is 0.5-2.5% of the mass of the biological enzyme;
the diluent comprises amino polymer, antibacterial agent and water;
the mass ratio of the antibacterial agent to the biological enzyme is 1: (10-20);
the mass ratio of the multifunctional active biological enzyme to the diluent is 1: (40-80);
the antibacterial agent is vitamin C reduced graphene oxide.
2. The use of a multi-potent active biological enzyme according to claim 1, wherein said polysaccharide is a homopolysaccharide.
3. The use of a multi-functional active biological enzyme according to claim 2, wherein the homopolysaccharide is selected from at least one of cellulose, glycogen, starch, chitin.
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