CN111286499A - Composite air purification composite biological enzyme and preparation method thereof - Google Patents

Composite air purification composite biological enzyme and preparation method thereof Download PDF

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Publication number
CN111286499A
CN111286499A CN201910540414.6A CN201910540414A CN111286499A CN 111286499 A CN111286499 A CN 111286499A CN 201910540414 A CN201910540414 A CN 201910540414A CN 111286499 A CN111286499 A CN 111286499A
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enzyme
biological enzyme
biological
composite
liquid
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卢海平
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Shanghai Kangquan Environmental Technology Co Ltd
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Shanghai Kangquan Environmental Technology Co Ltd
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    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
    • B01D53/34—Chemical or biological purification of waste gases
    • B01D53/74—General processes for purification of waste gases; Apparatus or devices specially adapted therefor
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    • B01D—SEPARATION
    • B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
    • B01D53/34—Chemical or biological purification of waste gases
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    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
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    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
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    • C12Y101/03—Oxidoreductases acting on the CH-OH group of donors (1.1) with a oxygen as acceptor (1.1.3)
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    • C12Y110/03—Oxidoreductases acting on diphenols and related substances as donors (1.10) with an oxygen as acceptor (1.10.3)
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    • C12Y111/01—Peroxidases (1.11.1)
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    • C12Y302/01017—Lysozyme (3.2.1.17)
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Abstract

The invention relates to a composite air purification composite biological enzyme, which is a mixture consisting of biological enzyme, protease, glucose oxidase, ligninase, lipase and filler; aiming at the public places such as newly decorated offices, newly decorated schools, newly decorated families, furniture, schools, hotels, malls and the like, and different environment combination full spectrum optimal enzyme species such as kitchens, washrooms, shower rooms, factory workshops and the like, can play a role in the presence of various pollution sources, has high biological activity, degrades smells through a biological enzyme catalytic degradation function, inhibits the conversion of the smells, and achieves the aim of removing the smells, and the preparation method of the composite air purification composite biological enzyme is also provided.

Description

Composite air purification composite biological enzyme and preparation method thereof
Technical Field
The invention relates to the field of deodorant devices, in particular to a composite air purification composite biological enzyme and a preparation method thereof.
Background
Research has shown that Volatile Organic Compounds (VOCs) are one of the main pollutants in indoor air, and the kinds of indoor VOCs are increasingly used with various new chemicals. The main sources of indoor volatile organic compounds are indoor decorative materials and furniture, which can volatilize volatile organic compounds such as formaldehyde, benzene and the like; secondly, the pollution caused by the building and the harmful gas released by the building material; and thirdly, pollutants caused by indoor modern office equipment and household appliances. It is in indoor environment for a long time, and is easy to cause malignant diseases such as cancer, leukemia and the like.
Various deodorant agents for indoor use are disclosed in the prior art, for example, the invention disclosed in publication No. CN107261801A, which provides a deodorant agent for decoration pollution, including collagen, vesicle hydrogel, sodium chloride, titanium dioxide, lithocholic acid, surfactant and deionized water. Has the advantages of convenient use, disinfection and sterilization, air purification, moisture retention and dehumidification; for another example, chinese patent CN1552458A discloses a nano deodorant for air purification and a preparation method thereof, belonging to the field of nano technology. The nanometer deodorant powder is prepared by taking nanometer titanium dioxide powder, nanometer tourmaline powder, nanometer muscovite powder, sodium silicate, sodium benzoate, sodium chloride, polyacrylamide, hydroxyethyl cellulose and glycerol as raw materials, ball-milling, stirring, mixing and drying, the powder is taken as a basic raw material, and three forms of products, namely powder atomization, emulsion and water injection, can be prepared into products which are suitable for different occasions in the whole decoration process, can be doped, sprayed and coated, can remove indoor peculiar smell within 24 hours through actual use, has the removal rate of harmful substances such as indoor benzene, ammonia and formaldehyde of 60-80 percent, can be known by combining the prior art, and is mainly divided into two types by the current indoor deodorant technology, namely, firstly, the indoor deodorant is eliminated through the reaction of a synthetic preparation and a volatile organic compound, and has the advantages of low cost, but the reactant of the nanometer deodorant easily causes secondary pollution; firstly, adsorb volatile organic compound through techniques such as nanometer, photocatalyst, its advantage is can not cause secondary pollution, but the cost is higher, and the adsorption capacity undersize, and the action time is long, and the clearance is lower, consequently seeks a low cost, the fast, pollution-free technique of removing flavor that takes effect and becomes the focus of research at present.
Disclosure of Invention
The invention aims to solve the technical problem of providing a composite air purification composite biological enzyme and a preparation method thereof, so as to solve the defects in the prior art.
The technical scheme for solving the technical problems is as follows:
a compound biological enzyme for air purification is characterized in that: it is a mixture consisting of biological enzyme, protease, glucose oxidase, ligninase, lipase and filler;
the biological enzyme is one of lysozyme, amylase and oxidoreductase;
the filler is sodium polyacrylate, polyethylene glycol, xylitol, cane sugar or a composite multi-pond polymer;
the mass percentages of the components of the mixture are as follows: 7-12% of lysozyme, 10-15% of aleurone, 10-15% of oxidoreductase, 8-12% of protease, 11-14% of glucose oxidase, 8-13% of ligninase, 10-13% of lipase and 30-54% of filler, wherein the sum of the mass percentages of the components is 100%
The property of the compound biological enzyme is one of aqueous emulsion, microemulsion, powder, granule and block.
The invention also comprises a preparation method of the compound air purification compound biological enzyme, which is characterized by comprising the following steps:
1) selecting the activity of 500-1000U/mg according to different components of the pollution source of the polluted air cargo
The biological enzymes are enzyme seeds, and the biological enzymes are weighed according to the raw material proportion and are separated;
2) respectively adding the selected enzyme seeds into pure water 11 times the mass of the enzyme seeds at 10-30 ℃ for dissolving and precipitating, separating out supernatant, and respectively passing through a membrane with the pore diameter of 0.1-0.3MPa
0.005-0.010 μm ultrafiltration membrane to obtain enzyme with activity unit of 2000-2500U/mL and pH of 5-6
Liquid; further dissolving enzyme in the tailings by using an organic solvent, and mixing the enzyme solution into the tailings; said is provided with
The organic solvent is one of ethanol, ethyl enzyme, methanol, ethyl acetate and methyl isobutyl ketone;
3) fully stirring the enzyme liquid in the step (2) in a container at 45-55 ℃ for 33-42min to ensure that the enzyme liquid is
Mixing uniformly; standing at 25-30 deg.C for 2-6h, and passing through membrane with pore diameter of 0.1-0.3MPa
0.005-0.010 μm ultrafiltration membrane, concentrating and thickening at 25-30 deg.C to reach activity unit
2800 and 3000U/mL, passing the enzyme solution through the 0.005-0.010 μm ultrafiltration membrane again,
the activity unit of the enzyme is 30000-
Storing at-0 deg.C to obtain liquid compound biological enzyme;
4) putting the enzyme mixed solution obtained in the step (3) for 10-12 days, and carrying out ultrasonic atomization; collecting the atomized enzyme to obtain the final liquid product.
The invention has the beneficial effects that: aiming at the newly decorated offices, newly decorated schools, newly decorated families, furniture, schools, hotels, restaurants, shopping malls and other public places, kitchens, washrooms, shower rooms, factory workshops and other different environments, the air purification and odor removal system can combine a full spectrum of optimal enzymes, can play a role in the presence of various pollution sources, has high biological activity, and can degrade odor through a biological enzyme catalytic degradation function and inhibit odor conversion to realize the purpose of removing odor.
Detailed Description
The principles and features of this invention are described below in conjunction with examples which are set forth to illustrate, but are not to be construed to limit the scope of the invention.
A composite air purification composite biological enzyme is a mixture consisting of biological enzyme, protease, glucose oxidase, ligninase, lipase and filler;
the biological enzyme is one of lysozyme, amylase and oxidoreductase;
the filler is sodium polyacrylate, polyethylene glycol, xylitol, cane sugar or a composite multi-pond polymer;
the mass percentages of the components of the mixture are as follows: 7-12% of lysozyme, 10-15% of aleurone, 10-15% of oxidoreductase, 8-12% of protease, 11-14% of glucose oxidase, 8-13% of ligninase, 10-13% of lipase and 30-54% of filler, wherein the sum of the mass percentages of the components is 100%
The property of the compound biological enzyme is one of aqueous emulsion, microemulsion, powder, granule and block.
The preparation method of the compound air purification compound biological enzyme comprises the following specific steps:
(1) selecting biological enzymes with the enzyme activity of 500-;
(2) respectively adding the selected enzyme seeds into pure water with the mass being 11 times of that of the enzyme seeds for dissolving and precipitating at the temperature of 10-30 ℃, separating out supernatant, and then respectively passing through ultrafiltration membranes with the membrane pore diameter of 0.005-0.010 mu m under the condition of 0.1-0.3MPa to prepare enzyme liquid with the activity unit of 2000-2500U/mL and the pH value of 5-6; further dissolving enzyme in the tailings by using an organic solvent, and mixing the enzyme solution into the tailings; the organic solvent is one of ethanol, ethyl enzyme, methanol, ethyl acetate and methyl isobutyl ketone;
(3) fully stirring the enzyme liquid in the step (2) in a container at 45-55 ℃ for 33-42min to uniformly mix; standing for 2-6h at 25-30 ℃, passing through an ultrafiltration membrane with the membrane aperture of 0.005-0.010 mu m under the condition of 0.1-0.3MPa, concentrating and thickening the enzyme liquid reaching the activity unit of 2800-3000U/mL at 25-30 ℃, passing the enzyme liquid through the ultrafiltration membrane of 0.005-0.010 mu m again, refining to obtain the activity unit of 30000-6000000U/mL, emulsifying and storing the enzyme liquid and the enzyme liquid mixture at-0 ℃ to obtain the liquid composite biological enzyme;
(4) putting the enzyme mixed solution obtained in the step (3) for 10-12 days, and carrying out ultrasonic atomization; collecting the atomized enzyme to obtain the final liquid product.
The specific working principle is as follows:
the main components of indoor air pollution are formaldehyde, benzene, toluene and xylene, which are collectively called TVOC. The biological enzyme takes part in the protein decomposition reaction, the starch decomposition reaction and the oxidation-reduction reaction, the chemical combination reaction between the poly pond and pollutant molecules, the biological enzyme decomposes molecular chains of generated compounds and the like, thereby achieving the aim of removing pollution and odor. The high-efficiency composite biological enzyme preparation catalytic deodorization technology has unique treatment effect on pungent smell, and particularly has good degradation effect on toxic and harmful macromolecular compounds which are difficult to degrade. The method is an application of enzyme and enzyme technology in the field of environmental pollution treatment, can quickly perform catalytic oxidation on organic matters forming pollution in a short time, and can remove pollutants and the like with generated odor by opening a ring and breaking a chain to form micromolecular organic matters after the macromolecule organic matters are subjected to catalytic oxidation.
The biological enzyme of the invention contains a plurality of biological enzymes, one of lysozyme, amylase and oxidoreductase and one or a plurality of protease, glucose oxidase, ligninase and lipase, and the pollutants in the indoor air can be roughly divided into 4 types: first, formaldehyde; second, benzene series such as benzene, toluene, xylene, etc.; and thirdly, the total volatile organic compound TVOC. And fourthly, ammonia gas. Such as isovaleric acid, propyl crotonic acid, etc., can also be decomposed. The biological enzyme in the biological enzyme can generate neutralization and condensation reaction with formaldehyde, benzene, ammonia and the like in indoor pollutants, simultaneously inhibit the reduction of TVOC and eliminate free odor and toxic gas substances in the environment.
Specifically, the invention has the following advantages:
(1) the special selected biological enzyme contained in the product can generate neutralization and condensation reaction with active groups in odor substances, such as formaldehyde, benzene series substances, amino, ammonia and the like, simultaneously inhibit the formaldehyde and benzene series pollutants from being converted into harmless carbohydrate, carbon dioxide and water, eliminate toxic chemical substances free in the environment and cannot cause any secondary pollution.
(2) Realizes the optimized combination of biological enzymes, and the effect of the biological enzymes cannot be achieved by other smell removing agents. The odor removal and pollution removal performance of the composite material is evaluated by selecting a proper formula, and the composite material has good removal effect on various chemical components including ammonia gas, formaldehyde, benzene series, total volatile organic compounds TVOC and the like.
(3) The treatment efficiency is high. Compared with the common chemical method and biological method, the product of the invention has 100 times of degradation effect on the irritant substance compared with the traditional method, can rapidly remove the irritant chemical smell and purify the air.
(4) The adaptability is wider. The product of the invention can adapt to various temperature and pH ranges and can effectively play a role in a low-oxygen environment.
(5) Is more targeted. The product of the invention can adjust various formulas on the premise of main formula, and can be widely applied to different fields and smells with different components.
(6) The treatment cost is low. The product of the invention has the characteristics of treating both principal and secondary aspects of diseases, and has the lowest comprehensive treatment cost and dynamic investment cost and obvious treatment effect.
(7) A pure green environment-friendly product. The product of the invention is compounded by pure natural biological enzyme, does not contain any chemical medicine, does not contain components of a transgenic product, does not cause secondary pollution, is nontoxic and harmless to human bodies, and represents the future direction of development of the biological environment-friendly industry.
(8) No sense of discomfort, no stimulation, no taste and the like.
Therefore, the biological enzyme has good pollution removal effect, is pure green and environment-friendly, has advanced technology, and is suitable for industrial scale production and market application and popularization.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.

Claims (2)

1. A compound biological enzyme for air purification is characterized in that: it is a mixture consisting of biological enzyme, protease, glucose oxidase, ligninase, lipase and filler;
the biological enzyme is one of lysozyme, amylase and oxidoreductase;
the filler is sodium polyacrylate, polyethylene glycol, xylitol, cane sugar or a composite multi-pond polymer;
the mass percentages of the components of the mixture are as follows: 7-12% of lysozyme, 10-15% of aleurone, 10-15% of oxidoreductase, 8-12% of protease, 11-14% of glucose oxidase, 8-13% of ligninase, 10-13% of lipase and 30-54% of filler, wherein the sum of the mass percentages of the components is 100%
The property of the compound biological enzyme is one of aqueous emulsion, microemulsion, powder, granule and block.
2. The preparation method of the compound air purification compound biological enzyme according to claim 1, characterized by comprising the following steps:
1) selecting biological enzymes with the activity of 500-;
2) respectively adding the selected enzyme seeds into pure water with the mass being 11 times of that of the enzyme seeds at the temperature of between 10 and 30 ℃ for dissolving and precipitating, separating out supernatant, and then respectively passing through ultrafiltration membranes with the membrane pore diameter of between 0.005 and 0.010 mu m under the condition of between 0.1 and 0.3MPa to prepare enzyme liquid with the activity unit of 2000-2500U/mL and the pH value of 5-6; further dissolving enzyme in the tailings by using an organic solvent, and mixing the enzyme solution into the tailings; the organic solvent is one of ethanol, ethyl enzyme, methanol, ethyl acetate and methyl isobutyl ketone;
3) fully stirring the enzyme liquid in the step (2) in a container at 45-55 ℃ for 33-42min to uniformly mix; standing for 2-6h at 25-30 ℃, passing through an ultrafiltration membrane with the membrane aperture of 0.005-0.010 mu m under the condition of 0.1-0.3MPa, concentrating and thickening the enzyme liquid reaching the activity unit of 2800-3000U/mL at 25-30 ℃, passing the enzyme liquid through the ultrafiltration membrane of 0.005-0.010 mu m again, refining to obtain the activity unit of 30000-6000000U/mL, emulsifying and storing the enzyme liquid and the enzyme liquid mixture at-0 ℃ to obtain the liquid composite biological enzyme;
4) putting the enzyme mixed solution obtained in the step (3) for 10-12 days, and carrying out ultrasonic atomization; collecting the atomized enzyme to obtain the final liquid product.
CN201910540414.6A 2019-06-21 2019-06-21 Composite air purification composite biological enzyme and preparation method thereof Pending CN111286499A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114958305A (en) * 2022-03-25 2022-08-30 广州市番禺环境科学研究所有限公司 Dedusting agent and preparation method thereof
CN114984746A (en) * 2022-08-05 2022-09-02 山东建筑大学 System method and device for treating VOCs waste gas in disinfectant and aquaculture wastewater pool

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114958305A (en) * 2022-03-25 2022-08-30 广州市番禺环境科学研究所有限公司 Dedusting agent and preparation method thereof
CN114984746A (en) * 2022-08-05 2022-09-02 山东建筑大学 System method and device for treating VOCs waste gas in disinfectant and aquaculture wastewater pool

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