CN102526781B - Compound biological enzyme deodorant and preparation method thereof - Google Patents
Compound biological enzyme deodorant and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a compound biological enzyme deodorant and a preparation method thereof. The compound biological enzyme deodorant contains the ingredients consisting of one of laccase, hemicellulase and oxidoreductase and the mixture of proteinase, lipase, cellulose, pectinase and a filler and is prepared by carrying out the following working procedures of: enzyme selecting and compounding, standing and nitrification, low-temperature concentrating, molecular sieving, hierarchal filtering, fluidized bed low-temperature drying, molecular modification and immobilization, product forming,inspecting and packaging. The product prepared by the preparation method has the advantages of stable quality, remarkable deodorizing effect, longer keeping time, environment-friendliness, convenience in use and suitability for the air purification and deodorization of fields, such as the municipal facilities including public toilets, garbage dump stations, garbage transfer stations and the like,the public places including stations, guest houses, hotels and the like, the household facilities including kitchens, wash basins, shower compartments, siphon tubes or any drainage pipeline and the like, and the factory workshops and the like.
Description
Technical field
The invention belongs to the Environmental Biotechnology field, be specifically related to a kind of compound biological enzyme reodorant and preparation method thereof.
Background technology
At present, Chang Yong reodorant mainly contains following several types: physical deodorization agent, chemical deodorizing agent, microbiocidal reodorant, plant type reodorant and compound reodorant etc.
The physical deodorization agent is to carry out deodorizing by physical method, utilizes the physical properties of reodorant or foul smell, does not change the result of odor components, only changes the partial concn of foul smell, or perhaps relative concentration.Common the absorbing and deodorizing agent arranged, cover reodorant etc.Shortcoming is to be difficult to change fully the foul smell gaseous constituent, and people and animals, equipment and environment etc. are still had to a certain degree infringement.
Thereby the chemical deodorizing agent is to utilize oxidation, reduction decomposition, neutralization reaction, addition reaction, condensation reaction, ion exchange reaction etc. that the repugnant substance that produces is become the odorless material to destroy foul odors.Shortcoming is that investment is big, and operation cost is higher relatively, and particularly the product behind the chemical reaction has the possibility that causes new uncontrollable environmental pollution.The case that the pollution that causes owing to chemosynthesis reodorant in recent years causes happens occasionally, the somebody treats the preceding paragraph time spraying perfume or contained in the environment of reodorant of fragrance, symptoms such as skin pruritus, dizziness and cough can appear, the serious even asthma that can happen suddenly.The somebody itch dermatitis unbearably can occur once some fragrant reodorant of contact, even feels sick, vomits.These are all relevant with the pollution of chemical deodorizing agent.
Natural plant deodorant is adopt to extract that the natural disinfection deodorization factor is refining in the plant forms.Have antibacterial, sterilization and deodorizing effect, stenches such as ammonia, hydrogen sulfide are had good decomposition removal effect, can be used for purifying air and the peculiar smell processing of industries such as food medicine, solid useless processings, sewage disposal.Its effect changes the steric configuration of peculiar smell molecule, weakens the chemical combination key in the peculiar smell molecule, easily and the acidic buffer generation chemical reaction in other molecules and the vegetation water, generates tasteless, nontoxic material at last.Its deficiency is, plant deodorant extraction process complexity, and production cost is higher, and the deodorizing object is single, also may produce the chemical reaction that some are difficult to expect.
Biological deodorant is the metabolite that takes full advantage of beneficial microbe colony, adopts microbial fermentation, abstraction technique production, and it has the stench of imitating smelly sources such as obstruct ight soil and distributes.Its ultimate principle is to utilize microorganism that the repugnant substance in the dissolving water is absorbed in microorganism in health, makes a kind of process of its degraded by the Metabolic activity of microorganism.Microbial deodorization can be divided into three phases: the 1. dissolution process of foul gas, namely transfer to liquid phase by gas phase; 2. odor pollutant is adsorbed, absorbs by microorganism in the aqueous solution; 3. the odor pollutant that enters microorganism cells is that Institute of Micro-biology decomposes utilization as nutritive substance, and pollutent is removed.Yet, the pollutent that contains in the stink is a very complicated mixed system, single culture can't reach satisfied effect, be difficult to establish standard, the pollution factor that has by product after microbial metabolism is indeterminate, there is the environmental safety problem, and is not suitable for shortcomings such as extra heavy concentration foul smell.
Therefore, have exploitation and the use of the reodorant of efficient deodorizing effect and biological safety, become the domestic and international research focus, and have great market and ecological significance.Exploitation prozyme biological deodorant, research and development by the biological fixnig system, screening, make up the high-performance biotechnological formulation of efficient, cheapness, strong stress resistance, use safety, make various biological enzyme performance synergies, widen the kind that can handle pollutent, improving processing efficiency is the developing direction that the environment friendly biological formulation development is produced.
It is the broad-spectrum biological enzyme preparation that compound biological enzyme reodorant manufacture method of the present invention obtains this product performance, has special advantages.By containing multiple meticulously preferred synergy biological enzyme, the multiple foul smell composition of degraded can act synergistically; Accessible stench mineral compound mainly comprises sulfurous gas, hydrogen sulfide etc.; Accessible volatility stench organism mainly comprises sulfurous organic compound (mercaptan, thioether), phosphorus-containing matter, chlorinated organics etc.This product can be used for sewage, rubbish, efficient deodorizing product in the sludge treatment; But the common stink factor in also degrade in the effective environment voltaile fatty acid and the pollutent; Particularly bio-oxidation enzyme and lytic enzyme strengthen its deodorizing capability; Safe water based formulas and solid-state prescription, nontoxic to the person poultry safety, nonirritant; Not flammable, biodegradable, non-secondary pollution; Be applicable to normal temperature, optimal temperature is 10-50 ℃; Usually with water dilution 5-10 doubly, easy to use.
Summary of the invention
The object of the present invention is to provide a kind of compound biological enzyme reodorant and preparation method thereof, the product processes advanced person of this method preparation, material is easy to get, and cost is low, and enzyme is lived stable.
For achieving the above object, the present invention adopts following technical scheme:
A kind of component of compound biological enzyme reodorant is a kind of and proteolytic enzyme, lipase, cellulase, the polygalacturonase in laccase, hemicellulase, the oxydo-reductase, the mixture of filler;
Described filler is cyclodextrine, polyoxyethylene glycol, dextran, heparin or sucrose polymer;
The mass percent of each component is: proteinase 8-13%, lipase 9-15%, cellulase 10-15%, polygalacturonase 8-13%, laccase 12-15%, hemicellulase 9-12%, oxido-reductase 12-15%, filler 30-54%, each constituent mass percentage ratio sum is 100%.
Described compound biological enzyme reodorant is a kind of in aqua, aqueous emulsion, microemulsion, Powdered, particulate state, the bulk.
A kind of preparation method of compound biological enzyme reodorant comprises following operation: select enzyme composite, leave standstill nitrated, cryoconcentration, molecule screening, level is filtered, ebullated bed cryodrying, molecular modification and immobilization, moulding, check, packing.
The concrete steps of its preparation method are as follows:
(1) according to removal foul smell composition difference, selecting enzyme activity for use is enzyme at the biological enzyme of 500-1000U/mg, and each biological enzyme is pressed proportioning raw materials Weighing and material sorting;
(2) under 10-30 ℃, the pure water that the enzyme of selecting for use is added its 10 times of quality respectively dissolves, precipitates, separated liquid supernatant, under the 0.1-0.5MPa condition, be the ultra-filtration membrane of 0.005-0.015 μ m by membrane pore size respectively then, make the enzyme liquid of unit of activity 2000-2500U/mL, pH5-6; Enzyme during tailings is slagged tap with the further dissolved of organic solvent is mixed in the enzyme liquid;
Described organic solvent is a kind of in ethanol, methyl alcohol, ethyl acetate, the hexone;
(3) under 40-50 ℃, the enzyme liquid of step (2) is fully stirred 30-40min in container, make it to mix; Leave standstill 2-6h under 20-35 ℃, under the 0.1-0.5MPa condition, be the ultra-filtration membrane of 0.005-0.015 μ m by membrane pore size, concentrated thickening reaches the enzyme liquid of unit of activity 2500-3000U/mL under 25-35 ℃, this enzyme liquid is passed through again the ultra-filtration membrane of 0.005-0.015 μ m, making with extra care out unit of activity is enzyme mixed solution preservation under-5 ℃ of 20000-5000000U/mL, is liquid compound biological enzyme reodorant;
(4) the enzyme mixed solution drying of step (3) is handled, made solid state compound biological enzyme reodorant;
Described drying treatment is vacuum-drying, ultra red ray drying, fluidized drying, lyophilize or adds the stopping composition natural air drying;
The processing parameter of described fluidized drying is that solid concentration is 3-5%, and the sample introduction flow is 360mL/h, and inlet temperature is 40 ℃-50 ℃, and temperature out is 30 ℃～35 ℃.
Biological enzyme technology deodorizing mechanism: the stink major ingredients is hydrogen sulfide, thiomethyl alcohol, dimethyl sulfide, dimethyl disulfide etc., is referred to as total reduced sulfur, wherein also contains pollution substances such as indoles organic compound and hydrocarbon polymer.The sulfide composition complexity comprises inorganic sulfur and organosulfur etc.Biological enzyme reacts by participating in carrying out proteolysis reaction, amylolysis reaction and oxidized fibre, and polyose and the intermolecular combination reaction of foul smell, and the biological enzyme decomposition produces the molecular chain of malodorous compounds etc., thereby reaches the purpose of removing stink.Nitrogenous organic substance such as amine, indoles are emitted NH through elite biological enzyme ammonification
3, NH
3Can be oxidized to NO by biological enzyme
2 -, further be oxidized to NO by biological enzyme again
3 -The repugnant substance of sulfur-bearing is oxidized into sulfuric acid by biological enzyme after the biological enzyme decomposition discharges H2S, organic substance etc. are decomposed into CO by elite biological enzyme
2, H
2O.Efficient compound bio zymin catalyzing deodorizing technology has unique treatment effect to foul smell, especially poisonous and harmful, the macromolecular cpd that is difficult to degrade is had good degradation effect.This method is that enzyme and zymotechnic are in the application in environmental pollution improvement field, can carry out catalyzed oxidation to the organism that forms stink rapidly at short notice, macromolecule organic open loop chain rupture behind catalyzed oxidation forms small organic molecule, removes the pollution substance of the stink that produces etc.
Contain multiple biological enzyme in the biological enzyme reodorant of the present invention, be mainly a kind of in oxydo-reductase, transferring enzyme, lytic enzyme, lyase, isomerase, the ligase enzyme or some kinds, repugnant substance in the environment is broadly divided into three kinds: the one, and nitrogenous compound is as ammonia, Trimethylamine 99, pyridine etc.; The 2nd, sulfocompound is as hydrogen sulfide, thiomethyl alcohol, thioether etc.; The 3rd, organic acid and aldehyde compound are as formaldehyde, isovaleric acid, propyl group Ba Dousuan etc.Biological enzyme in the biological enzyme reodorant of the present invention can with repugnant substance in active group such as sulfydryl, amido, ammonia etc. neutralize, condensation reaction, suppress vitriol simultaneously and transform and generate hydrogen sulfide, eliminate the stink substance that is free in the environment.
At municipal administrations such as public lavatory, rubbish station, garbage transfer stations, public place such as station, hotel, families such as kitchen, hand washing sink, shower cabinet, siphon pipe or any drainage pipeline, and the optimum enzyme of the full spectrum of varying environment such as factory floor purifying air deodorizing combination, can when existing, multiple source of pollution play a role, the biological activity height, it is by biological enzyme degradation function degraded osmophore and suppress the foul smell conversion, realizes removing the purpose of stink.
(1) the contained elite biological enzyme of product of the present invention can with repugnant substance in active group such as sulfydryl, amido, ammonia etc. neutralize, condensation reaction, suppress vitriol simultaneously and transform generation hydrogen sulfide, pollution substance in the foul smell is converted into harmless carbohydrate, elimination is free in the stink substance in the environment, can not cause any secondary pollution.
(2) realized the optimum combination of biological enzyme, its effect is that other reodorant are inaccessiable.Select suitable prescription, its deodorization is assessed, the various odor pollutants that comprise ammonia, hydrogen sulfide, thiomethyl alcohol, thioether etc. are shown good removal effect.
(3) processing efficiency height.Product of the present invention is compared with general chemical process and biological method, is 100 times of traditional method to the degradation effect of repugnant substance, can remove stink rapidly, purifies air.
(4) adaptability is wider.Product of the present invention can adapt to various temperature and pH scope, also can effectively play a role in low-oxygen environment.
(5) more targeted.Product of the present invention can be adjusted multiple formulations under master side's prerequisite, can be widely used in different field, heterogeneity foul smell.
(6) treatment cost is low.Product of the present invention has the advantages that to treat both principal and secondary aspect of disease, and comprehensive regulation cost and dynamic investment cost are minimum, and regulation effect is remarkable.
(7) pure Green Product.Product of the present invention all adopts the pure-natural biological enzyme to be composited, and does not contain any pharmaceutical chemicals, does not also contain the transgenic product composition, can not cause secondary pollution, and is nontoxic to human body, representing the following direction of biological environmental production industry development.
Therefore, not only deodorizing effect is good for biological enzyme reodorant of the present invention, advanced technology, and suitable industrial-scale production and market application.
Each processing step and advantage are in the manufacture method of the present invention:
1) selects the enzyme complex process
The optimum enzyme of the full spectrum of varying environment combination can play a role when multiple source of pollution exist, the biological activity height, and it passes through biological enzyme degradation function degraded osmophore and suppresses foul smell to transform, and realizes the purpose of removal stink.A kind of pollutent can dispose corresponding enzyme and effectively handle.Be mainly a kind of in oxydo-reductase, transferring enzyme, lytic enzyme, lyase, isomerase, the ligase enzyme or some kinds.
Soak: 10～30 ℃ of operating ambient temperatures; The storage tank jar; Stir: treatment temp: 40～50 ℃; Treatment time: 30～40min; Extracting: can is stored: 25kg, 120kg bucket packing, be stored in 0 ℃-20 ℃ the warehouse, and store 1 year stationary phase.The enzyme compound formulation can be preserved under 37 ℃ 90 days, and this enzyme and compound formulation thereof do not contain the chemical disinfection composition, can not cause secondary pollution to environment.Said preparation is nontoxic, non-stimulated.
In compound process, the monomer type and the synthesis technique that use when selecting different living enzyme polymerization, make final synthetic enzyme agent in molecular weight, molecular weight distribution and chain structure in the aqueous solution, by the synthetic polymer prozyme agent with comb-type structure of free-radical polymerized principle.2) leave standstill digestion process
This technology is under aerobic or anaerobic conditions material to be carried out in the standing and reacting tank body, and the time is 2-6 hour, and temperature is controlled at 20-35 ℃, reaches and stablizes the effect of complex enzyme power, and each is component balanced to make the product intermediate.And the quality that reduces the residual slag charge of preparation process again and have nothing to do with reactant.The oneself of coenocorrelation system keeps balance.Can make the product stabilization, make it be difficult for to change, safe and harmlessization, and improved the effect of product.
3) low-temperature concentration process
Adopt membrane sepn to concentrate and combine with the production environment air-conditioning, form a kind of energy-saving low-temperature concentration technology, material keeps producing the clean environment of knot, per hour composite extracting solution is concentrated to 10 times of ratios of volume from initial mass concentration.Concentrate operating ambient temperature 25-35 ℃, concentrate temperature of charge control below 50 ℃, the energy consumption of this low-temperature concentration process is energy-conservation 30%, and service life of equipment prolongs; Tail washings is back in the extractor under the atmospheric pressure state automatically repeatedly, concentrates while extracting, the difficult problem of temperature drift when solving enzyme liquid and concentrating.4) molecular sieve division technique
Utilize molecular sieve enzyme liquid can be separated with non-utilization organic matter.The enzyme molecule itself has the protein tridimensional network, and big molecule resistance by the hole on this reticulated structure time is bigger, and resistance was less when small molecules passed through.When selecting the sifting bed that molecular weight 20,000-5,000,000 U varies in size for use, according to molecular weight size ordered distribution.Molecular sieve can improve activity and the uncommon selectivity of enzyme, effectively guarantees the enzyme quality.
5) level filtration process
A centrifugation: remove the large granular impurity and the mineral-type solids precipitation thing that in composite enzyme liquid, contain.B vibrates sub-sieve: the cotton-shaped impurity of colloid of further removing suspended substance and reaction process generation through the material of centrifugation.C adsorption precipitation: make the enzyme molecule fully obtain activating release and being retained in the aqueous based carrier through adding activator (Calcium of intensifiens etc.) material after above-mentioned supply step is finished.The smart filter of D ultrafiltration: the smart filter of employing ultra-filtration technique is further removed colloidalmaterial and the denatured protein in the material, makes enzyme liquid obtain clarification, keeps molecular weight 20,000 U-5,000,000 U.Can adopt series connection or super rate unit in parallel, in airtight environment, carry out ultrafiltration and can significantly reduce oxygen to the influence of enzyme liquid quality, the enzyme work of product intermediate is kept more completely with effect.This cell process is not pursued single enzyme preparation purity, but (concentration) of raising prozyme compares vigor.
This technology makes zymin material enzyme quality in the course of processing improve greatly, effectively removes the many unfavorable factors that influence quality, and the product vigor that obtains is higher, normal temperature state enzyme is lived in losing and is lacked, and can guarantee the production high-level efficiency, smooth operation is beneficial to the prolongation in maintenance of the equipment and work-ing life.
6) ebullated bed low temperature drying technology
Material after above-mentioned art breading adopts boiling drier, claims fluidized-bed again, generally is made up of well heater, ebullated bed main frame, cyclonic separator, sack cleaner, induced draft fan, operator's console.According to the different in kind of material, can match cyclonic separator or sack cleaner on demand.
Boiling drier is to utilize air after heat exchanger heats, form hot blast and enter main frame through the valve plate distribution, wet stock enters drying machine from feeder, because the effect of blast, material forms boiling state in drying machine, and extensively contact with warm air, determine that the optimum operation processing parameter is that solid concentration is 3-5%, the sample introduction flow is 360mLh, inlet temperature is 40 ℃-50 ℃, temperature out is 30 ℃～35 ℃, and enzyme rate of loss about 2% alive is finished drying materials at the short period cold condition.This technology guarantees effectively that enzyme is lived and does not suffer a loss.
7) molecular modification and immobilization technology
Increase prozyme biotechnological formulation stay in grade.Introducing by chemical group or remove changes the protein covalent structure.Under relatively mild condition, make a kind of protein play specific reaction with some chemical reagent in the mode that can control, thereby cause the chemically changed of single amino acids residue or its functional group generation covalency.There are a plurality of reactive groups in the modifier molecule, can to form multiple spot crosslinked with enzyme.Make the native conformation of enzyme produce " rigidity " structure.Make enzyme molecular structure be in a kind of equilibrium state at last, closely orderly conformation is maintained.Enhanced stability, the inactivation of minimizing enzyme.It is efficiently quick by this technology the directed target function of prozyme to be improved greatly to polluting dirty decomposition.Removing influences enzyme quality unfavorable factor, the biological enzyme deodorising product that obtains efficiently expressing.
Stable condition and optimum reaction condition optimal pH are 4.5, temperature is 45 ℃.Modifier is: a kind of in cyclodextrine, polyoxyethylene glycol (PEG), dextran (dextran), heparin (heparin), the sucrose polymer (Ficoll).The activator that adopts: a kind of in CNBr method, glutaraldehyde method, the azide method.
Particulate state compound enzymic preparation immobilization technology: adopt the covalent attachment method to connect forming chemical covalent linkage on the zymoprotein molecule between the lip-deep reactive group of functional group and solid support, be connected firmly between enzyme and carrier, be difficult for taking place enzyme and come off, satisfactory stability and reusability are arranged.It adopts carrier to comprise natural polymer (Mierocrystalline cellulose, agarose, starch, glucose gel, collagen and derivative etc.) and inorganic support (sintered glass, metal oxide etc.).Well keep or prolonged the enzyme activity unit time through the prozyme material of this workshop section art breading.
8) preparations shaping technology
This prozyme biological deodorant, above-mentioned technological process are the essential characteristics of present technique, but can be according to different needs, and all or part of method in the reservation technology is to adapt to formulation.Can be prepared into aqua, solid formulation (comprising block tablet), agent gas.
Multi-form can be suitable for the problem that exists in the process of consumption, by forming technique enzymic activity is further protected, and prolongs the preservation period of preparation within the specific limits.Studies show that of storage stability in the matrix, reduced the susceptibility to the preservation temperature, improved oxidation resistance, and the enzyme activity transformation period is prolonged.
In sum, this invention is compared with present prior art, and production technique design of the present invention is tight, with high content of technology, the excellent product quality of producing is the new application in the product of environmental protection field of a kind of novel modern enzyme and zymotechnic, has integrate innovations characteristics.At municipal administrations such as public lavatory, rubbish station, garbage transfer stations, public place such as station, hotel, families such as kitchen, hand washing sink, shower cabinet, siphon pipe or any drainage pipeline, and the optimum enzyme of the full spectrum of varying environment such as factory floor purifying air deodorizing combination, can when existing, multiple source of pollution play a role, the biological activity height, it is by biological enzyme degradation function degraded osmophore and suppress the foul smell conversion, realize removing the purpose of stink, and environmentally friendly, do not produce secondary pollution.
Embodiment
Embodiment is described in conjunction with the embodiments:
Further describe advantage of the present invention and characteristics below in conjunction with embodiment.Following examples only are exemplary, and those skilled in the art can make apparent modification on the basis, and these modifications also are included within the scope of the invention.
According to foul smell composition difference, selecting enzyme activity a kind of or some kinds of enzymes more than 500U/mg for use is enzyme, with each biological enzyme component by weight ratio Weighing, material sorting, dissolution precipitation, leave standstill that digestion, separation and Extraction, mixing are stirred, refining purifying, level filter, concentrate thickening, screening, drying, molecular modification and immobilization, get product.Wherein, the kind of zymin: oxydo-reductase, transferring enzyme, lytic enzyme, lyase, isomerase, ligase enzyme.Selecting for use of zymin: according to the characteristic of handling foul smell, selected zymin is purified and is composite, and sorbent material is gac, silica gel, diatomite, aluminum oxide and various ion exchange resin etc.; Extraction agent is alcohols, acetate esters and first isobutyl ketone etc.Biological enzyme formulation is aqueous, aqua, aqueous emulsion, microemulsion or Powdered, particulate state or bulk.
Embodiment 1
According to the disclosed concrete grammar of claim, prepare a kind of biological enzyme reodorant, this kind reodorant belongs to rubbish station series of biologic enzyme reodorant.Undertaken by above-mentioned embodiment, it comprises each component of following weight ratio:
| Composition | Proteolytic enzyme | Lipase | Cellulase | Polygalacturonase | Laccase | Filler |
| Content | 10-13% | 12-15% | 12-15% | 10-12% | 12-15% | 30-44% |
The vigor of various enzymes is all more than 500U/mg in the above component.
When above-mentioned biological enzyme reodorant was used for the foul smell processing of rubbish station, the biological enzyme that contains in the biological enzyme reodorant is catalyzed degradation osmophore and the conversion of inhibition foul smell effectively, thereby removes stink.
Refuse landfill rubbish can produce a large amount of foul smell in the landfill process, influence surrounding enviroment.It is that domestic waste is concentrated the place of filling out the reason processing that rubbish is filled out Li Chang, owing to here topple over the process of processing from the rubbish that each rubbish point ships, especially in summer, autumn, organism in the domestic refuse decomposes harmful odorous gas such as producing a large amount of ammonias, hydrogen sulfide, amine, aromatic hydrocarbons, alcohols, organic acid, indoles, be full of rubbish and filled out the whole space of managing the field, foul smell causes ambient air contamination.About 100 0m2 of landfill district scope of work.Adopt this compound biological enzyme scavenging agent deodorization liquid, by the rubbish surface of atomizing spray to landfill, and in the rubbish toppling process, spray fast, thereby reach the effect of deodorizing.A eliminates the stink that organism decomposes and animal excrement produce in the domestic refuse; B, the dissipation insect: mosquito, fly blow and larva are had certain killing action, and minimizing mosquito, fly gather; C can increase compactness, thereby increases the reason ability etc. of filling out that rubbish is filled out the reason field.D, biological enzyme formulation are pure biological product, can 100% biological degradation, use safety, and can not cause secondary pollution to environment; E, because biological enzyme formulation is the prozyme that contains multiple difference in functionality enzyme, when being used for the domestic refuse of complicated component, more show it act on the deodorizing of many target spots than powerful.Under conditions such as suitable temperature, humidity, in rubbish or percolate, can keep the efficient catalytic effect; F has certain storage effect spraying continuously when using, thereby can reduce consumption but do not reduce result of use; G, biological enzyme formulation with water be solvent can be according to actual needs by the arbitrary proportion dilution, as long as be equipped with suitable spray appliance, can use very easily in various different places such as the pour point in refuse tip, diafiltration liquid pool, roads.
Embodiment 2
According to the disclosed concrete grammar of claim, be prepared a kind of biological enzyme reodorant, this kind reodorant belongs to public place series of biologic enzyme reodorant such as station, hotel.Undertaken by above-mentioned embodiment, it comprises each component of following weight ratio:
| Composition | Proteolytic enzyme | Lipase | Cellulase | Polygalacturonase | Hemicellulase | Filler |
| Content | 9-12% | 10-13% | 10-13% | 8-10% | 9-12% | 40-54% |
The vigor of various enzymes is all more than 500U/mg in the above component.
When above-mentioned biological enzyme reodorant was used for family and the processing of public place foul smell, the biological enzyme that contains in the biological enzyme reodorant is catalyzed degradation osmophore and the conversion of inhibition foul smell effectively, thereby removes stink.
Embodiment 3
According to the disclosed concrete grammar of claim, be prepared a kind of biological enzyme reodorant, this kind reodorant belongs to family's series of biologic enzyme reodorant such as kitchen, hand washing sink, shower cabinet, siphon pipe or any drainage pipeline.Undertaken by above-mentioned embodiment, it comprises each component of following weight ratio:
| Composition | Proteolytic enzyme | Lipase | Cellulase | Polygalacturonase | Oxydo-reductase | Filler |
| Content | 8-10% | 9-12% | 10-14% | 10-13% | 12-15% | 36-51% |
The vigor of various enzymes is all more than 500U/mg in the above component.
When above-mentioned biological enzyme reodorant was handled for foul smell such as kitchen, hand washing sink, shower cabinet, siphon pipe or any drainage pipelines, the biological enzyme that contains in the biological enzyme reodorant is catalyzed degradation osmophore and the conversion of inhibition foul smell effectively, thereby removes stink.
Embodiment 4
According to the disclosed concrete grammar of claim, be prepared a kind of biological enzyme reodorant, this kind reodorant belongs to factory floor series of biologic enzyme reodorant.Undertaken by above-mentioned embodiment, it comprises each component of following weight ratio:
| Composition | Proteolytic enzyme | Lipase | Cellulase | Polygalacturonase | Oxydo-reductase | Filler |
| Content | 9-11% | 12-15% | 11-14% | 9-12% | 12-15% | 33-47% |
The vigor of various enzymes is all more than 500U/mg in the above component.
When above-mentioned biological enzyme reodorant was used for the processing of factory floor foul smell, the biological enzyme that contains in the biological enzyme reodorant is catalyzed degradation osmophore and the conversion of inhibition foul smell effectively, thereby removes stink.
Above-described embodiment as can be seen, biological enzyme reodorant of the present invention shows good removal effect for the various odor pollutants that comprise ammonia, hydrogen sulfide, thiomethyl alcohol, thioether etc., simultaneously for comprise in refuse container, lavatory, the automobile, the common common peculiar smell environment of smoke, also show tangible deodorizing effect.By synergy and the optimum combination of biological enzyme, all show excellent deodorization effect for common foul gas and peculiar smell environment.This product is the compound bio zymin, when being used for the production plant organic exhaust gas of complicated component, more show it act on the deodorizing of many target spots than powerful.In the wide temperature of envrionment temperature 10-50, possess under the humidity condition, can keep katalysis efficiently, accelerate organic decomposition; Can 100% biological degradation.Environmentally safe, efficient, nontoxic; Even enter in the eye or injured skin, get final product with flushing with clean water, operator safety is harmless.
Embodiment 5
Compound biological enzyme is directly added by a certain percentage in smelly water body, and compound biological enzyme can be present in water body and the mud for a long time by from immobilization and compound immobilization role, is difficult for running off.Improvement phase dosage (3 days): strengthen to add according to the water yield and with reference to the ratio of sludge quantity; Maintenance phase dosage (1 week): proportionally add.Can no longer add after up to standard.The follow-up maintenance phase (180-360 days): ratio adds as required, and purpose is to activate the metabolic process of the efficient probiotics of microorganisms in water, thereby keeps the long-term decontamination effect improving of water body.Rapidly and efficiently catalyzed degradation produces the pollutent of stink, removes stink; Shorten the processing cycle, thereby reduce running cost; Improve the surrounding enviroment quality, eliminate peculiar smell, prevent the breeding of flies and mosquitoes; Can efficiently remove simultaneously the pollutent in the mud, reduce sludge quantity, reduce Facilities Construction, and can make treatment system under wide temperature, bring into play the effect of biological degradation pollutent; A kind of reinforcement to nature restorability and self-purification capacity.Compare with other treatment technologies, really realize unpowered or microkinetic consumption, meet the developing direction of energy-saving and emission-reduction and low-carbon economy.
Embodiment 6
(1) soaks haydite after 1-1.5 hours with 8% hydrochloric acid soln, put dried solution and add clear water (preferably pure water) washing to not residual.(2) recording the PH that haydite is immersed in the water is below the neutrality.Best between 5-6.(3) the haydite drying after will washing (is preferably in 100-140OC).Make the central no water molecules of hole and other non-reacted residue of haydite.(4) dried haydite is soaked in the enzyme liquid at least 6-7 hours, Best Times 12 hours wherein, slowly stirs 0.5-1 hour (12-24 rev/mins of stirring velocitys).(5) will handle 0.5 hour by the way transformation that vacuum is taken out through the pottery material in the immersion of enzyme liquid.(6) haydite is taken out from enzyme liquid or enzyme liquid is entered other storage tank after, carry out the haydite drying, temperature must not surpass 45oC.(7) enzyme liquid carries out secondary and applies mechanically the immersion haydite.Enzyme activity is detected with the sampling of dried finished products enzyme filler in (operation is pressed said process) (8).(9) with dried enzyme filler dress post (or pack), standby.
The present invention is novel, the biological enzyme reodorant that makes with this invention, be applied to municipal administrations such as public lavatory, rubbish station, garbage transfer station, public place such as station, hotel, families such as kitchen, hand washing sink, shower cabinet, siphon pipe or any drainage pipeline, and in the field such as factory floor purifying air deodorizing, the deodorizing good purification, efficient height and cost are low, and stability is strong, and is easy and simple to handle.
Above-mentioned only is several specific embodiment of the present invention; should be understood that; design concept of the present invention is not limited thereto; for those skilled in the art; under the prerequisite that does not break away from the principle of the invention; can also make some improvements and modifications, allly utilize this design that the present invention is carried out the improvements and modifications of unsubstantiality, all should belong to the behavior of invading protection domain of the present invention.
Claims (1)
1. the preparation method of a compound biological enzyme reodorant is characterized in that: the component of described compound biological enzyme reodorant is a kind of and proteolytic enzyme, lipase, cellulase, the polygalacturonase in laccase, hemicellulase, the oxydo-reductase, the mixture of filler;
Described filler is cyclodextrine, polyoxyethylene glycol, dextran, heparin or sucrose polymer;
The mass percent of each component is: proteinase 8-13%, lipase 9-15%, cellulase 10-15%, polygalacturonase 8-13%, laccase 12-15%, hemicellulase 9-12%, oxido-reductase 12-15%, filler 30-54%, and each constituent mass percentage ratio sum is 100%;
Described compound biological enzyme reodorant is a kind of in aqua, aqueous emulsion, microemulsion, Powdered, particulate state, the bulk;
The preparation method comprises following operation: select enzyme composite, leave standstill nitrated, cryoconcentration, molecule screening, level is filtered, ebullated bed cryodrying, molecular modification and immobilization, moulding, check, packing; Concrete steps are as follows:
(1) according to removal foul smell composition difference, selecting enzyme activity for use is enzyme at the biological enzyme of 500-1000U/mg, and each biological enzyme is pressed proportioning raw materials Weighing and material sorting;
(2) under 10-30 ℃, the pure water that the enzyme of selecting for use is added its 10 times of quality respectively dissolves, precipitates, separated liquid supernatant, under the 0.1-0.5MPa condition, be the ultra-filtration membrane of 0.005-0.015 μ m by membrane pore size respectively then, make the enzyme liquid of unit of activity 2000-2500U/mL, pH5-6; Enzyme during tailings is slagged tap with the further dissolved of organic solvent is mixed in the enzyme liquid;
Described organic solvent is a kind of in ethanol, methyl alcohol, ethyl acetate, the hexone;
(3) under 40-50 ℃, the enzyme liquid of step (2) is fully stirred 30-40min in container, make it to mix; Leave standstill 2-6h under 20-35 ℃, under the 0.1-0.5MPa condition, be the ultra-filtration membrane of 0.005-0.015 μ m by membrane pore size, concentrated thickening reaches the enzyme liquid of unit of activity 2500-3000U/mL under 25-35 ℃, this enzyme liquid is passed through again the ultra-filtration membrane of 0.005-0.015 μ m, making with extra care out unit of activity is enzyme mixed solution preservation under-5 ℃ of 20000-5000000U/mL, is liquid compound biological enzyme reodorant;
(4) the enzyme mixed solution drying of step (3) is handled, made solid state compound biological enzyme reodorant;
Described drying treatment is vacuum-drying, ultra red ray drying, fluidized drying, lyophilize or adds the stopping composition natural air drying;
The processing parameter of described fluidized drying is that solid concentration is 3-5%, and the sample introduction flow is 360mL/h, and inlet temperature is 40 ℃-50 ℃, and temperature out is 30 ℃～35 ℃.
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| CN109045999A (en) * | 2018-08-21 | 2018-12-21 | 南京创灵环保科技有限公司 | Compound biological enzyme deodorant and preparation method thereof |
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| CN110642488A (en) * | 2019-10-14 | 2020-01-03 | 哈尔滨鸿盛房屋节能体系研发中心 | Portable biodegradable deodorizing and dirt absorbing bag for feces |
| CN111567576A (en) * | 2020-05-07 | 2020-08-25 | 重庆杏磊湾环境治理有限公司 | Production process of biological enzyme fly-killing adjuvant |
| CN112156645B (en) * | 2020-09-23 | 2021-04-27 | 柳州市宝净科技有限公司 | Composite biological enzyme deodorant and preparation method thereof |
| CN114853526A (en) * | 2021-01-20 | 2022-08-05 | 北京云图生态工程有限公司 | Multi-enzyme active peptide combined fertilizer and preparation method thereof |
| CN113716989A (en) * | 2021-07-28 | 2021-11-30 | 安徽国祯环卫科技有限公司 | Complex enzyme preparation for kitchen waste compost and preparation and application method thereof |
| CN114231505A (en) * | 2021-12-16 | 2022-03-25 | 中晟普能环保科技(北京)有限公司 | Biological enzyme preparation beneficial to removing peculiar smell and preparation method thereof |
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