KR101454895B1 - Media for cultivating microorganism including microporus structure and manufacturing method thereof - Google Patents

Abstract

The present invention relates to a medium of microporous structure and a method of manufacturing the same. More specifically, the present invention is characterized in that a microporous medium capable of promoting the activity of environmental microorganisms is prepared, and the plant-based biomass (raw material) is subjected to a base treatment. The medium according to one embodiment of the present invention can be used for the fermentation / extinction treatment of food waste, activated sludge in sewage / sewage / wastewater or livestock manure, and the fermentation / extinguishment of dirt using the medium of the present invention, And the odor generated in the fermentation / extinction process is small, and the durability of the medium for microorganism culture is excellent, so that it can be used for a long period of time.

Description

[0001] The present invention relates to a medium for microbial culture having a microporous structure and a method for producing the microporous structure,

The present invention relates to a medium for microorganism culture having a microporous structure and a method for producing the same.

The present invention relates to a medium for microorganism culture which can be used for the fermentation / disappearance treatment of dirt and a method for producing the medium.

Generally, there are food waste which accounts for 25 to 35% of household waste, activated sludge in sewage, sewage and wastewater, and animal manure, which contain protein carbohydrate and fat, . Therefore, efforts to reduce or purify dirt are continuing, and in particular, the fields that use environmental microorganisms are developing in various ways.

On the other hand, microorganisms are microscopic organisms with a size of less than 0.1 mm, mainly composed of single cells or mycelium, and are composed of algae, bacteria, protozoa, fungi, yeast, And viruses that can be called enemy creatures. They can grow anywhere in the world with temperature and humidity conditions and are closely related to human life. In particular, utilizing the peculiar properties of microorganisms, it is utilized not only as a biological resource in foods, medicines, etc., but also as a biological reaction function in environmental technologies such as water quality, waste and soil.

In recent years, genetic engineering methods have been introduced in the development of microbial strains, and special microorganisms capable of decomposing organic matter according to pollution targets such as water quality, waste and soil have been promoted, and improvement of efficiency of pollution reduction and removal by individual amplification has been promoted. In this case, the microorganism is referred to as an 'environmental microorganism', and exists in a range of several micro micro to several tens micro micro. The environmental microorganisms can perform the function of decomposing organic matter introduced into the reaction tank for promoting digestion, such as a fermentation / extinction tank for food waste disposal, an activated sludge reaction tank for treating sewage, sewage, wastewater, or livestock manure.

Generally, the environmental microorganisms are kept constant when the conditions of temperature, humidity, and nutrients are satisfied, and the organic matter is decomposed according to the amount of the treated water and the activity of the environmental microorganisms, so that pollutants can be treated. However, the treatment efficiency varies depending on the number of environmental microorganisms and the right ignition, the generated sludge and by-products can be reduced, and the amount of the sludge and byproduct generated can be reduced to reduce the capacity of the reactor, do. Therefore, in order to increase the amount of microorganisms generated, a medium capable of promoting the activity of microorganisms contained in a certain volume of space is required. The size of environmental microorganisms is mostly sub-micrometer units, and it is necessary to amplify the habitat by condensation of micro-pores which can expand the effective surface area of microorganisms per unit volume in medium production.

In general, the 500 Å pore serves to rapidly transport the adsorbed molecule from the outside to the entrance, and it is called as admission, diffusion, or transport pore. As an international classification, macro pores It is named. It is called meso pore because it belongs to the middle of the pores which are dominated by the pores of 200 to 500 Å in the porous solid and below the pores and participate in such microorganism formation and adsorption. Micropores having a diameter of 9 to 20 angstroms are called micropores because they belong to micropores in the pores that participate in microorganism culture and adsorption. Pores less than 8 Å are called submicropores, with pores having roughly the same order of magnitude as the molecular size. There is also a demand for the production of a medium composed of fine pores having a uniform size depending on the intended use of the slurry.

However, it is still difficult to produce a medium having a microporous structure of a uniform size in the production of a medium which is a habitat of microorganisms. Another problem in the process of fermenting and eliminating food waste, activated sludge in livestock wastewater, or livestock manure by using medium is that the medium itself is durably durable and is crumbled. As described above, the effect of reducing by-products after fermentation and extinction and the method of manufacturing a medium with enhanced durability are expected to have great industrial value in the relevant industrial fields.

Korean Patent No. 0737122

An object of the present invention is to provide a microbial culture medium having microporous structure which can promote the generation and activity of microorganisms and increase durability in order to increase treatment efficiency of dirt and reduce sludge and byproducts generated.

Another object of the present invention is to provide a method for producing a medium for microorganism culture, which comprises the step of base treatment and high temperature sterilization so that the microbial production and activity can be promoted and the microporous structure having excellent durability can be formed.

The present invention provides a medium for microbial culture characterized in that a plant-based biomass is treated with a basic solution to form micropores and sterilized.

The present invention also provides a method for producing the medium for microorganism culture.

In addition, the present invention provides a method for fermenting and extinguishing dirt under conditions of a temperature of 30 to 50 ° C. and a humidity of 30 to 70% by mixing the microorganism-forming medium and dirt.

The micropores formed in the method for producing a medium for microorganism culture according to an embodiment of the present invention have a diameter of 8 to 15 탆.

The plant-based biomass that can be used in the present invention preferably includes cellulose, and the plant-based biomass including cellulose is preferably at least one selected from wood chips (sawdust), fallen leaves, waste wood, plant fibers and processed products thereof But is not limited thereto.

The base treatment for forming micropores is characterized in that 100 to 400 ml of a basic solution of 1 to 5% by weight is added to 100 g of the plant-based biomass, followed by stirring and immersion. The base treatment temperature is preferably 50 to 60 ° C, but is not limited thereto.

In addition, the sterilization treatment is preferably performed at a temperature of 150 to 200 DEG C with stirring and roasting.

The medium prepared by the method of the present invention can be packaged in an aseptic pack and stored semi-permanently. It is also possible to use an enzyme, a microorganism, or both capable of promoting the fermentation / extinction reaction of the soil immediately before packaging in an aseptic pack. Can be added. The microorganism may further contain 5 to 10 parts by weight of enzyme, microorganism or both, based on 100 parts by weight of the medium for microorganism culture. The added enzyme is preferably at least one selected from carbohydrase, protease, and lipolytic enzyme, but is not limited thereto. As the microorganism to be added, it is preferable to add at least one selected from Bacillus, Pseudomonas, Actinomycetes and Nitromonas, but not limited thereto. When both the enzyme and the microorganism are contained, the content ratio thereof is preferably 1: 99 to 99: 1 by weight, and the added step is preferably a step of adding the medium for microorganism culture after sterilization treatment to the sterilized pack .

In addition, an adsorbent may be further included to reduce the odor generated during the fermentation / extinction treatment of dirt using the medium according to one embodiment of the present invention. The amount of the adsorbent to be added may be 10 to 30 parts by weight of the adsorbent per 100 parts by weight of the medium for microbial culture, and the adsorbent is preferably activated carbon, zeolite or activated clay. Not limited.

Preferably, the dirt is food waste, sludge of wastewater, sewage or wastewater, or animal manure, and the mixing ratio of the microorganism-forming medium and the dirt is in a weight ratio of 1: 1 to 10: 1. When the content of the medium for microbial culture is less than twice the amount of dirt, the fermentation and extinction are not performed well, and when the content exceeds 5 times, the economical efficiency and the space efficiency are inferior.

The present invention relates to a medium for microorganism culture having a microporous structure and a method for producing the same. The microporous medium for microbial culture provided by the present invention promotes the activity of microorganisms and can efficiently ferment and extinguish the activated sludge of food waste, sewage, sewage, wastewater or animal manure.

In addition, the medium for microorganism culture produced according to the production method of the present invention is characterized by having well-formed microporous structure. Since the microporous structure has a large surface area, the microorganisms can be smoothly formed even in a small space, and the amount of the fermentation / disappearance of the soil is increased. In addition, the medium for microorganism culture according to the present invention has a deodorizing effect by adsorbing odor due to the fermentation of dirt.

Therefore, the fermentation / extinguishing treatment of food waste, sludge of sewage, sewage, wastewater, or animal manure using the medium according to the present invention has very excellent weight reduction effect, odorlessness and economy, It is very easy to handle, so it can be easily used at home.

1 is a scanning electron microscope (SEM) photograph showing the pore structure of a microorganism-forming medium (Example 1) formed by base treatment and heat sterilization on woody matter.
FIG. 2 is a scanning electron microscope (SEM) photograph showing the pore structure of a regular wood grain piece (Comparative Example 1). FIG.
3 is a scanning electron microscope (SEM) photograph showing the pore structure of a microorganism-mixed woody wood grain (Comparative Example 2).
Fig. 4 is an illustration showing the ATP concentration ratio to COD in the medium for microbial culture of Example 1 and Comparative Examples 1 and 2. Fig.
5 is an illustration showing the ATP concentration ratio to TN in the medium for microorganism culture of Example 1 and Comparative Examples 1 and 2. Fig.
FIG. 6 is an exemplary view showing a result of weight loss experiment of a food waste using a microorganism-forming medium (Example 1) formed by base treatment and heat sterilization on woody matter.
FIG. 7 is an exemplary view showing a result of weight loss test of food waste using a general wood-based piece (Comparative Example 1); FIG.
FIG. 8 is an exemplary view showing a result of weight loss experiment of food waste using a microbial wood type wood particle (Comparative Example 2); FIG.

The present invention relates to a medium for microorganism culture and a method for producing the same, characterized in that microbes are formed by treating the plant-based biomass with a basic solution and sterilized.

The plant-based biomass may be any of those capable of supplying organic nutrients, and is preferably a plant-based biomass including cellulose, more preferably a wood-based grain (sawdust), a fallen leaf, a waste wood; And a processed product of vegetable fiber, woody wood (sawdust), fallen leaves, waste wood or vegetable fiber, and most preferably woody wood (sawdust) is used. Sawdust (sawdust) is composed of cellulose structure which can supply organic nutrients, and it is a very desirable material for medium because it is easy to deform pore structure.

A method for producing a medium for microbial culture having a microporous structure capable of promoting the generation and activity of microorganisms

(1) crushing or cutting one or more plant-based biomasses having similar physical properties of an organic compound;

(2) washing the plant-based biomass, which has been crushed or cut in the step (1), to remove impurities;

(3) homogenizing the property of the plant based biomass from which the impurities have been removed in the step (2);

(4) subjecting the plant-based biomass homogenized in the step (3) to a base treatment to form micropores; And

(5) sterilizing the micropores in the step (4).

In step (1), the plant-based biomass should be capable of providing a space in which microorganisms can smoothly live. Therefore, it is preferable that the plant-based biomass uses a material having similar physicochemical properties.

In the step (1), it is preferable that the crushing or cutting of the plant-based biomass is uniformly crushed or cut to a size of 1 to 5 cm. If the size of the plant-based biomass is less than 1 cm, the microbial culture medium is crumbled and crumbled during the fermentation / disappearance process of the soil. Therefore, the amount of by-products or sludge generated in the fermentation / extinction of dirt increases, and when it is more than 5 cm, the fine pores are not formed well, which makes them unsuitable for microorganisms, especially environmental microorganisms.

In step (2), it is preferable that the confined and cut biomass of the plant is immersed in washing water (tap water) and impregnated with stirring for 1 to 2 hours.

For 100 g of the plant-based biomass, 0.5 to 2.0 L of wash water is preferably used. It is preferred that the biomass obtained by solid-liquid separation from the washing water is naturally dried at room temperature for 2 to 3 days.

In the step (3), it is preferable that homogenization of the property is performed in a size of 1 to 5 cm.

In the step (4), the base treatment for microporous formation is preferably carried out by stirring and immersing the plant-based biomass in a basic solution at 50 to 60 ° C for 10 to 15 hours. The basic solution is preferably injected with 100 to 400 ml of a basic solution of 1 to 5% by weight per 100 g of the medium, but is not limited thereto.

When the concentration of the basic aqueous solution is less than 1% by weight, the porosity of the plant-based biomass hardly changes. When the concentration of the basic aqueous solution exceeds 5% by weight, the biomass of the plant itself is abnormally generated.

The sterilization treatment in step (5) is preferably carried out at a temperature of 150 to 200 ° C for 3 to 5 hours with stirring and roasting, but is not limited thereto. Preferably, the sterilized medium for microorganism culture is naturally dried in a clean room. Further, after the step (5), the prepared medium may be further added with enzyme, microorganism or both. The enzyme may further include 5 to 10 parts by weight of an enzyme, microorganism or both, based on 100 parts by weight of the medium for microorganism culture. Examples of the enzyme that can be added include at least one of carbohydrase, protease, But is not limited thereto. In addition, as the microorganism which can be added, it is preferable to add at least one selected from Bacillus, Pseudomonas, Actinomycetes and Nitromonas, but it is not limited thereto.

When the enzyme and the microorganism are both included, the content ratio thereof is preferably 1:99 to 99: 1 by weight, and the added step is preferably added before packaging the microorganism-forming medium into the sterile pack.

The adsorbent may further include 10 to 30 parts by weight of adsorbent per 100 parts by weight of the medium for microbial culture in order to reduce the odor generated during the fermentation and extinction of the dirt. The adsorbent is preferably activated carbon, zeolite, activated clay, but is not limited thereto.

The microorganism-forming medium prepared by the production method of the present invention is packaged in an aseptic pack and is stored at 30 to 40 ° C, and semi-permanent storage is possible, so that there is no limitation on the expiration date.

The microorganisms which can be inhabited in the medium for microorganism culture of the present invention are not limited, but preferable microorganisms include bacillus which decomposes protein, fat or starch, pseudomonas which can decompose petrochemical organic matter or cause carbon assimilation, It is preferably, but not limited to, actinomycetes for decomposing the fibers or nitromonas for decomposing or nitriding substances of ammonia.

In addition, the present invention provides a method for treating soil by fermentation or extinction using a medium for microorganism culture. More particularly, the present invention relates to a method for producing microorganisms, And

And culturing the microorganism at a temperature of 30 to 50 DEG C and a humidity of 30 to 70%.

It is preferable that the dirt is food waste, sludge of sewage, sewage or wastewater, or animal manure, but the mixing ratio of the medium and the dirt for the microorganism is not limited to the ratio of the medium to the dirt Is preferably in a weight ratio of 1: 1 to 10: 1, but is not limited thereto. When the amount of medium for microbial culture is less than one time the amount of dirt, the fermentation / extinction is not performed well. If the amount of medium exceeds 10 times, the amount of medium becomes too large compared with dirt, resulting in poor economical efficiency and space efficiency.

When the amount of the medium to be administered once is 100 parts by weight, the medium of the present invention is first fermented and destroyed with 100-1,000 parts by weight of the medium. Thereafter, when about 10 to 100 parts by weight of dirt is added daily, the dirt can be fermented and destroyed for 6 to 8 months without adding any further medium.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are merely illustrative of the present invention and that the scope of the present invention is not limited by these embodiments.

Example  1. Preparation of medium for microbial treatment with base treatment and high temperature sterilization.

100 kg of plant biomass such as woody wood, wood, sawdust, waste fiber, fallen leaves, etc. were crushed and cut into 2 cm size, washed with 2 × 10 ³ l of tap water, and dried naturally for 2 days. The dried plant-based biomass was kept at a size of 2 cm to homogenize the properties of the plant-based biomass. 100 kg of homogeneous plant-based biomass was added to 200 L of 3% ammonia water at 55 캜 and stirred and immersed for 12 hours. Then, the mixture was sterilized by stirring in a reaction vessel at 180 ° C. for 4 hours, and dried in a clean room at room temperature for 3 days to prepare a medium for microorganism culture.

Example 2. Preparation of medium for microbial treatment with base treatment and high temperature sterilization added with enzyme and activated carbon.

In order to accelerate the fermentation and extinction of dirt in 1 kg of the microorganism-forming medium prepared in Example 1, 50 g of bacillus and 200 g of activated carbon as an adsorbent were added to effectively remove odors.

Example 3. Analysis of Microporous Structure of Medium for Microbial Culture.

(One) Example  The microbial preparation medium prepared in 1

The pore structure of the medium for microorganism culture prepared in Example 1 of the present invention was measured by Scanning Electron Microscopy (SEM) in units of 15.0 kW x 3.00 K and 10 m. It was confirmed that the medium for microbial culture prepared in Example 1 was formed with 10 탆 outer and inner pores while maintaining the cellulosic tissue structure. The formation of such micropores is a structure well suited for microbial culture. In other words, it has been confirmed that the medium for microorganism culture having the structure in which the effective surface area is enlarged due to the condensation of micropores forms a structure very suitable for microbial generation and culture due to the spatial expansion of microorganisms.

(2) Comparative Example  1: medium made of ordinary wood (hereinafter referred to as " Comparative Example  1 or Comparative Example  1 medium ').

In Comparative Example 1, the pore structure of a medium for microorganism culture, which is made of a commercially available general wood, was measured by Scanning Electron Microscopy (SEM) in units of 15.0 kW × 3.00 K, 10 μm.

The medium of Comparative Example 1 was a typical conifer-woody wood with a cellulose-based structure, and some pores were identified. As compared with the medium for microbial culture prepared in Example 1 of the present invention, as compared with the medium of Comparative Example 2, micropores were observed in a relatively small amount, and the microbes could be formed and formed in the fermentation / extinction reaction of food wastes It was judged that there was a possibility of insufficiency.

(3) Comparative Example 2: Microorganism-mixed woody medium for microbial culture (hereinafter referred to as " Comparative Example  2 medium ').

As Comparative Example 2, the structure of the medium was measured in units of 15.0 kW × 3.00 K and 10 μm using Scanning Electron Microscopy (SEM) using the microorganism-containing media for commercial microorganism mixed wood type.

The medium of Comparative Example 2 is a product produced and sold in Japan, and is a medium into which microorganisms are injected into a woody matter. Although it is not similar to the embodiment of the present invention, it has been confirmed that microbes are present in a large amount, and microbes can be formed and formed in the fermentation / extinction reaction of the soil. Materials with a rounded structure between the media were similar to yeast structures (see Figures 1-3)

Example  4. Conduct biological analysis.

The contents of Chemical Oxygen Demand (COD), Total Nitrogen (TN) and Adenosine Triphosphate (ATP) were compared with respect to the chemical properties of the medium.

The microorganism-forming medium of Example 1 and Comparative Examples 1 and 2 was ground in a mortar and sieved with a standard of 0.075 mm (200 mesh), and dried at 105 ° C.

The medium for the microbial culture of Example 1 and Comparative Examples 1 and 2 was precisely weighed and placed in 100 ml of a 1% (v / v) sulfuric acid solution. After thorough mixing, the supernatant was collected and analyzed.

(1) Chemical oxygen demand ( COD )

The media A, B, and C of Example 1 and Comparative Examples 1 and 2 were subjected to sulfuric acidification, and a constant amount of potassium permanganate was added thereto. The resulting mixture was heated in a water bath for 30 minutes. The amount was measured using HACH (DR ~ 4000).

(2) Total nitrogen  ( TN )

The nitrogen compounds in the medium for microbial culture of Example 1 and Comparative Examples 1 and 2 were decomposed with organic matter at 120 ° C in the presence of potassium persulfate and oxidized to nitrate ions and then the ultraviolet absorbance was measured under acidic conditions to obtain nitrogen Was measured using HACH (DR ~ 4000).

(3) Adenosine triphosphate ( ATP )

 The medium for microorganism culture of Example 1 and Comparative Examples 1 and 2 was dissolved in 10 ml of a 1% sulfuric acid solution and 20 占 퐇 of a supernatant of a solution diluted 100 times with distilled water was sampled and placed in a measuring container DISPOTUBE (12 mm x 55 mm) And measured by a biochemiluminescence method using luciferase Luciferin using DKK TOA's AF-50 at a measurement temperature range of 5 to 35 ° C. The measurement results are shown in Table 1 below. (See Figures 3 and 4)

Table 1. Measurement results of COD, TN and ATP of the medium for microbial culture of Example 1 and Comparative Examples 1 and 2.

The concentrations of COD and TN in the medium were in the range of 100 to 1250 mg / g and 13 to 50 mg / g, and the concentration range of ATP was 30 to 53 pg / g. As shown in Table 1, the ratio of ATP / COD and ATP / TN showed that the medium for microorganism culture prepared in Example 1 of the present invention had a higher value than the medium for microorganism culture prepared in Comparative Examples 1 and 2 Able to know.

Example 5. Comparative analysis of fermentation and decay of food waste.

The food wastes fermentation extinction device used in the experiment was operated at a temperature of 30 to 50 ° C and a humidity of 30 to 70% using a reactor having a treating capacity of 2 kg per day. The amount of food waste put into the food waste for household use and restaurant was changed to 120 to 1,150 g per day for 10 days. The change in the weight of the food waste depends on the amount of food to be added and the amount of residue mixture (medium + Garbage) were measured. The mediums for microbial culture of Example 1 and Comparative Examples 1 and 2 used in Example 5 were added at a rate of 1,500 g once at the beginning of the experiment. Thereafter, food waste of the same condition was administered to each of the reaction vessels injected with media for microorganism culture of Example 1 and Comparative Examples 1 and 2 at intervals of one day, and in each of the days of the same reference Example 1, Comparative Examples 1 and 2 The weight of the food waste reduced by the medium for microorganism culturing of the food waste was measured and the weight loss efficiency (%) of the food waste was calculated according to the following formula (1).

<Formula 1>

The weight of residual food waste in Equation 1 is the weight of the residual mixture (weight of medium + weight of pure food garbage), excluding the weight of the initially charged medium (1500g).

(One) Example  1 for the microorganism-forming medium

The total amount of food waste collected during the 10 days was 6,710 g cumulative weight and the final residual amount was 2,070 g. Except for the weight of the microorganism-forming medium of Example 1 which was injected at the beginning of the experiment, the weight of food waste remained 570 g. Therefore, it was confirmed that the weight loss efficiency of food waste by the medium for microorganism culture of Example 1 was 92%.

Table 2 is a table showing the amount of food waste (g), accumulated amount of food waste (g), and amount of residual mixture (g) per day to confirm the effect of reducing the food waste by the medium for microbial culture of Example 1.

(2) For the microorganism-forming medium of Comparative Example 1

The total amount of food waste was 6,710 g cumulative weight, and the final residual amount was 3,980 g. Since the weight of the final residual amount includes the weight (1500g) of the medium for microbial culture of Comparative Example 1 which was initially injected, the pure residual amount after the fermentation disappearance of the input food waste is 2,480g. Therefore, the food waste reduction efficiency by the medium for microorganism culture of Comparative Example 1 was calculated by Equation (1) to be 63% efficiency.

Table 3 is a table showing the amount of food waste (g), accumulated amount of food waste (g), and amount of residual mixture (g) per day in order to confirm the effect of reducing the food waste by the medium for microbial culture of Comparative Example 1.

(3) Comparative Example  2 for the microorganism-forming medium

As a result of the experiment on the reduction of food waste using the medium for microbial culture of Comparative Example 2, the total amount of food waste was 6,710 g in cumulative weight, and the final residual amount (g) was 2500 g. Therefore, since the amount of food waste remaining pure is 1000 g, it can be seen that the efficiency of reducing food waste by the medium for microbial culture of Comparative Example 2 is 85%.

Table 4 is a table showing the amount of food waste (g), accumulated amount of food waste (g), and amount of residual mixture (g) per day to confirm the effect of reducing the food waste by the medium for microbial culture of Comparative Example 2.

Claims (13)

Treating the plant-based biomass with a basic solution to form micropores;
Sterilizing the micropores to prepare a medium for microorganism culture;
After the sterilization treatment, one or more enzymes selected from carbohydrases, proteases, and lipolytic enzymes; One or more microorganisms selected from Bacillus, Pseudomonas, Actinomycetes and Nitromonas; Or a mixture of the enzyme and the microorganism in an amount of 5 to 10 parts by weight based on 100 parts by weight of the microorganism-forming medium;
The method comprising the steps of: The method according to claim 1,
Wherein the formed micropores have a diameter of 8 to 15 占 퐉. The method according to claim 1,
Wherein the plant-based biomass comprises cellulose. The method of claim 3,
Wherein the plant-based biomass including cellulose is at least one selected from the group consisting of wood chips (sawdust), fallen leaves, waste wood, vegetable fibers, and processed products thereof. The method according to claim 1,
Wherein the basic solution is prepared by injecting 100 to 400 ml of a basic solution of 1 to 5% by weight based on 100 g of the plant-based biomass, followed by stirring and dipping. The method according to claim 1,
Wherein the treatment of the basic solution is carried out at 50 to &lt; RTI ID = 0.0 &gt; 60 C. &lt; / RTI &gt; The method according to claim 1,
Wherein the sterilization treatment is performed at a temperature of 150 to 200 DEG C with stirring and roasting. delete The method according to claim 1,
With respect to 100 parts by weight of the microorganism-forming medium,
Characterized in that 10 to 30 parts by weight of at least one adsorbent selected from the group consisting of active carbon, zeolite and activated clay is added after the sterilization treatment. A medium for microbial culture prepared by the method according to any one of claims 1 to 7 and 9. Mixing a medium for microbial culture and dirt according to claim 10; And
And culturing the microorganism at a temperature of 30 to 50 DEG C and a humidity of 30 to 70%. 12. The method of claim 11,
Wherein the dirt is food waste, sludge in sewage, sewage or wastewater, or livestock manure. 12. The method of claim 11,
Wherein the mixture ratio of the microorganism-forming medium and the dirt is 1: 1 to 10: 1 by weight.

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