CN107988200A - A kind of biological activated carbon and preparation method thereof - Google Patents

A kind of biological activated carbon and preparation method thereof Download PDF

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Publication number
CN107988200A
CN107988200A CN201711361278.1A CN201711361278A CN107988200A CN 107988200 A CN107988200 A CN 107988200A CN 201711361278 A CN201711361278 A CN 201711361278A CN 107988200 A CN107988200 A CN 107988200A
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activated carbon
preparation
biological activated
biological
sewage
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郑迅荟
郑宇杰
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YUSHAN SANQING ACTIVATED CARBON FACTORY
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YUSHAN SANQING ACTIVATED CARBON FACTORY
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    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00Treatment of water, waste water, or sewage
    • C02F1/28Treatment of water, waste water, or sewage by sorption
    • C02F1/281Treatment of water, waste water, or sewage by sorption using inorganic sorbents
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00Biological treatment of water, waste water, or sewage
    • C02F3/34Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/14Enzymes or microbial cells immobilised on or in an inorganic carrier

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Water Supply & Treatment (AREA)
  • Environmental & Geological Engineering (AREA)
  • Hydrology & Water Resources (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Inorganic Chemistry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention discloses a kind of biological activated carbon and preparation method thereof, the preparation method of the biological activated carbon comprises the following steps:1) bacteria selection, domestication, expansion culture and compounding, obtain composite bacteria;2) pretreatment of activated carbon;3) preparation of biological activated carbon:Pretreated activated carbon and composite bacteria are uniformly mixed, it is fully dry when 24~27 DEG C of constant temperature incubations 35~40 are small.The biological activated carbon specific surface area of the present invention is big, adsorption capacity is strong, it is good to the removal effect of COD in sewage, preparation process is simple, production cost is low, composite flora on biological activated carbon can pointedly degrade main COD components in sewage, the biomembrane formed also has certain adsorption function, so that biological activated carbon possesses COD absorption biomembrane, the dual absorption property of activated carbon adsorption carrier, extend the service life of biological activated carbon, reduce the dosage of activated carbon in sewage disposal process, market application value is high.

Description

A kind of biological activated carbon and preparation method thereof
Technical field
The present invention relates to a kind of biological activated carbon and preparation method thereof.
Background technology
With the deep development of modern industry, industrial sewage, Exhaust gas species are also more and more, COD in sewage Component becomes to become increasingly complex.COD (Chemical Oxygen Demand) is also known as chemical oxygen consumption (COC) for COD, is used for The amount of reducing substances aoxidized is needed in reflection sewage, at river pollution and trade effluent Quality Research and sewage In the operational management for managing factory, it is an organic pollution parameter that is important and can comparatively fast measuring.
At present, technical field of sewage uses activated carbon as absorption carrier mostly, activated carbon adsorption pollutant it is same When can also adsorb microorganism in environment, microorganism builds up within a certain period of time, multiply can be formed in the environment organic Thing has the biomembrane of degradation capability.Since the microorganism of activated carbon adsorption derives from natural environment, the growth and breeding of strain The non-intellectual such as ability, degradation property is very big, causes time for naturally taming different in size, forms the time disunity of biomembrane, The combination of biomembrane and activated carbon is unstable, is easily influenced and is destroyed be subject to temperature, pollutant concentration, pH value in environment, Possibly even also have pathogenic, and the biomembrane that the Institute of Micro-biology naturally adsorbed of activated carbon is formed is finer and close, can influence activity The absorption property of macropore and part mesoporous in carbon structure.
Therefore, it is necessary to develop a kind of new bioactivity Carbon Materials.
The content of the invention
It is an object of the invention to provide a kind of biological activated carbon and preparation method thereof.
The technical solution used in the present invention is:
A kind of preparation method of biological activated carbon, comprises the following steps:
1) bacteria selection, domestication, expansion culture and compounding:Major pollutants species and content selection in sewage Corresponding strain, is first tamed and expands culture respectively, then is compounded, and obtains composite bacteria;
2) pretreatment of activated carbon:Dipping pretreatment is carried out to activated carbon with hydrochloric acid, then Activated carbon separation is come out, fully It is dry;
3) preparation of biological activated carbon:Pretreated activated carbon and composite bacteria are uniformly mixed, 24~27 DEG C of constant temperature It is fully dry when culture 35~40 is small, obtain biological activated carbon.
Culture medium described in step 1) is beef extract fluid nutrient medium or beef extract solid medium, pH=7.2~7.5, The composition of the beef extract fluid nutrient medium is:Beef extract:0.3%;Peptone:1.0%;NaCl:0.5%, the ox The composition of meat extract solid medium is:Beef extract:0.3%;Peptone:1.0%; NaCl:0.5%;Agar:1.5%~ 2.0%.
Also added with least one of (NH4) 2S04, extraction from yeast powder, glucose in culture medium described in step 1).
Strain described in step 1) is at least one of bacillus, pseudomonad.
Strain described in step 1) is bacillus.
Activated carbon described in step 2) is at least one of coconut husk charcoal, carbo lignius, coal quality charcoal, fruit shell carbon.
The shape of activated carbon described in step 2) is at least one of cellular, column, graininess, powdered.
The mass ratio of activated carbon, composite bacteria described in step 3) is 1: (0.8~1.2).
Effective viable bacteria content >=2 × 109 in biological activated carbon described in step 3)/gram.
Biological activated carbon using the present invention carries out sewage disposal and comprises the following steps:
1) take genuine sewage according to 1~5g/L additive amount add biological activated carbon, by adjusting sewage pH values (3~ 10), the temperature (10~40 DEG C) and incubation time (12~72h) of incubator, determines the optimum biological activated carbon of sewage disposal Additive amount, sewage disposal pH value, degradation temperature and degradation time;
2) according to the optimum treatment process parameter of COD degradation in the definite sewage of step 1), sewage plant sewage is carried out Advanced treating.
The beneficial effects of the invention are as follows:The biological activated carbon specific surface area of the present invention is big, and adsorption capacity is strong, in sewage The removal effect of COD is good, and preparation process is simple, and production cost is low, and the composite flora on biological activated carbon can pointedly drop COD components main in sewage are solved, the biomembrane formed also has certain adsorption function so that biological activated carbon possesses COD absorption biomembrane, the dual absorption property of activated carbon adsorption carrier, extend the service life of biological activated carbon, reduce The dosage of activated carbon in sewage disposal process, market application value are high.
Embodiment
A kind of preparation method of biological activated carbon, comprises the following steps:
1) bacteria selection, domestication, expansion culture and compounding:Major pollutants species and content selection in sewage Corresponding strain, is first tamed and expands culture respectively, then is compounded, and obtains composite bacteria;
2) pretreatment of activated carbon:Dipping pretreatment is carried out to activated carbon with hydrochloric acid, then Activated carbon separation is come out, fully It is dry;
3) preparation of biological activated carbon:Pretreated activated carbon and composite bacteria are uniformly mixed, 24~27 DEG C of constant temperature It is fully dry when culture 35~40 is small, obtain biological activated carbon.
Preferably, the culture medium described in step 1) is beef extract fluid nutrient medium or beef extract solid medium, pH= 7.2~7.5, the composition of the beef extract fluid nutrient medium is:Beef extract:0.3%;Peptone:1.0%;NaCl: 0.5%, the composition of the beef extract solid medium is:Beef extract:0.3%;Peptone:1.0%;NaCl:0.5%;Fine jade Fat:1.5%~2.0%.
It is further preferred that also added with (NH4) 2S04, extraction from yeast powder, glucose in culture medium described in step 1) At least one of.
Preferably, the strain described in step 1) is at least one of bacillus, pseudomonad.
It is further preferred that the strain described in step 1) is bacillus.
Still further preferably, the strain described in step 1) is aerobic denitrification bacillus, bacillus subtilis, lichens In bacillus, dichloropyridine degraded bacillus, colloid bacillus cereus, bacillus subtilis, bacillus licheniformis at least It is a kind of.
Preferably, the activated carbon described in step 2) is at least one of coconut husk charcoal, carbo lignius, coal quality charcoal, fruit shell carbon.
Preferably, the shape of the activated carbon described in step 2) for cellular, column, graininess, it is powdered at least one Kind.
Preferably, the aperture of the activated carbon described in step 2) is less than 200nm.
Preferably, the activated carbon described in step 3), the mass ratio of composite bacteria are 1: (0.8~1.2).
Preferably, effective viable bacteria content >=2 × 109 in the biological activated carbon described in step 3)/gram.
The present invention is made further explanation and description with reference to specific embodiment.
Embodiment 1:
Sewage is certain food processing factory's waste water, and COD is nitrate nitrogen, selected strain for aerobic denitrification bacillus, Bacillus subtilis and bacillus licheniformis.
1) bacteria selection, domestication, expansion culture and compounding:Dirt is gathered near the sewage discharge mouth of certain food processing factory Water or pedotheque, inoculate on the beef extract fluid nutrient medium that nitric acid potassium concn is 500mg/L, in 30 DEG C of enrichments on shaking table Culture 4 days, then by 5% inoculum concentration, by tame nutrient solution be inoculated into nitric acid potassium concn be 600mg/L beef extract Liquid Culture On base, in 30 DEG C of enrichment cultures on shaking table 4 days, increase 100mg/L every time according still further to the concentration of substrate potassium nitrate, carry out circulation and tame and docile Change, until the concentration of potassium nitrate be 1000mg/L, then the strain separating in domestication liquid is come out, in 30 DEG C of standing constant temperature incubations 60 it is small when, obtain aerobic denitrification bacillus, then carry out degraded nitrate nitrogen experiment selection there is stronger nitrate nitrogen degradation function Aerobic denitrification bacillus, then it is uniformly mixed with the bacillus subtilis of no antagonism and bacillus licheniformis, Aerobic denitrification bacillus, bacillus subtilis, the mass ratio of bacillus licheniformis are 1: 1: 1, obtain composite bacteria.
2) pretreatment of activated carbon:By coal quality powdered active carbon be added in the hydrochloric acid of 0.1mol/L immersion 24 it is small when, Again with water backwash 60 minutes, it is clear to be washed till water, then Activated carbon separation is come out, when 45 DEG C of dryings 2 are small;
3) preparation of biological activated carbon:Pretreated activated carbon and composite bacteria in mass ratio 1: 1 is uniformly mixed, 27 When DEG C constant temperature incubation 40 is small, when 50 DEG C of dryings 4 are small, biological activated carbon is obtained.
A certain amount of food processing factory production waste water (major pollutants are nitrate nitrogen) is chosen, adds single creature bacterium respectively Agent, single-activity charcoal and biological activated carbon manufactured in the present embodiment, dosage are 2%, carry out COD degradation rate tests.
The biological activated carbon of the present invention reaches the degradation rate of nitrate nitrogen in sewage 98.56%, and treatment effect is better than single Microbial bacterial agent or activated carbon are added, being primarily due to biological activated carbon, there are activated carbon adsorption, biodegradation and biology at the same time The highly effective reaction processes such as absorption, and the single processing for applying activated carbon is better than the processing of single application microbial bacterial agent, mainly Because the adhewsive action of activated carbon makes microorganism growth rate and stability of a relatively high, the absorption and removal of nitrate nitrogen can be promoted.
Embodiment 2:
Sewage is certain herbicide plant produced waste water, and COD is dichloropyridine, and selected strain is to be dropped with dichloropyridine Solve dichloropyridine degraded bacillus, colloid bacillus cereus, bacillus subtilis and the bacillus licheniformis of function.
1) bacteria selection, domestication, expansion culture and compounding:Dirt is gathered near the sewage discharge mouth of certain herbicide factory Water or pedotheque, inoculate on the beef extract fluid nutrient medium that dichloropyridine concentration is 500mg/L, in 35 DEG C of richnesses on shaking table Collection culture 3 days, then by 3% inoculum concentration, by tame nutrient solution be inoculated into dichloropyridine concentration be 600mg/L beef extract liquid On culture medium, in 30 DEG C of enrichment cultures on shaking table 4 days, increase 100mg/L every time according still further to the concentration of substrate dichloropyridine, carry out Circulation domestication, until the concentration of dichloropyridine be 1000mg/L, then the strain separating in domestication liquid is come out, and perseverances are stood in 35 DEG C When temperature culture 48 is small, dichloropyridine degraded bacillus is obtained, then carry out degraded dichloropyridine experiment selection there is stronger dichloro The dichloropyridine degraded bacillus of pyridine degradable function, then by its colloid bacillus cereus with no antagonism, withered grass gemma Bacillus and bacillus licheniformis are uniformly mixed, dichloropyridine degraded bacillus, colloid bacillus cereus, bacillus subtilis, The mass ratio of clothing bacillus is 1: 1: 1: 1, obtains composite bacteria;
2) pretreatment of activated carbon:By coconut shell powder shaped activated carbon be added in the hydrochloric acid of 0.1mol/L immersion 20 it is small when, Again with water backwash 45 minutes, it is clear to be washed till water, then Activated carbon separation is come out, when 45 DEG C of dryings 6 are small;
3) preparation of biological activated carbon:Pretreated activated carbon and composite bacteria in mass ratio 1: 1.2 is uniformly mixed, When 25 DEG C of constant temperature incubations 37 are small, when 45 DEG C of dryings 6 are small, biological activated carbon is obtained.
A certain amount of herbicide plant produced waste water (major pollutants are dichloropyridine) is chosen, adds single creature respectively Microbial inoculum, single-activity charcoal and biological activated carbon manufactured in the present embodiment, dosage are 2%, carry out COD degradation rate test.
The biological activated carbon of the present invention reaches the degradation rate of dichloropyridine in sewage 52.83%, and treatment effect is better than single One addition microbial bacterial agent or activated carbon, being primarily due to biological activated carbon, there are activated carbon adsorption, biodegradation and life at the same time The highly effective reaction processes such as thing absorption, and the single processing for applying activated carbon is better than the processing of single application microbial bacterial agent, mainly It is because the adhewsive action of activated carbon makes microorganism growth rate and stability of a relatively high, the absorption of dichloropyridine can be promoted and gone Remove.
Above-described embodiment is the preferable embodiment of the present invention, but embodiments of the present invention and from above-described embodiment Limitation, other any Spirit Essences without departing from the present invention with made under principle change, modification, replacement, combine, simplification, Equivalent substitute mode is should be, is included within protection scope of the present invention.

Claims (10)

  1. A kind of 1. preparation method of biological activated carbon, it is characterised in that:Comprise the following steps:
    1) bacteria selection, domestication, expansion culture and compounding:Major pollutants species and content selection in sewage corresponds to Strain, first tamed respectively and expand culture, then compounded, obtain composite bacteria;
    2) pretreatment of activated carbon:Dipping pretreatment is carried out to activated carbon with hydrochloric acid, then Activated carbon separation is come out, it is fully dry It is dry;
    3) preparation of biological activated carbon:Pretreated activated carbon and composite bacteria are uniformly mixed, 24~27 DEG C of constant temperature incubations 35~40 it is small when, it is fully dry, obtain biological activated carbon.
  2. 2. preparation method according to claim 1, it is characterised in that:Culture medium described in step 1) is trained for beef extract liquid Support base or beef extract solid medium, pH=7.2~7.5, the composition of the beef extract fluid nutrient medium are:Beef extract: 0.3%;Peptone:1.0%;NaCl:0.5%, the composition of the beef extract solid medium is:Beef extract:0.3%;Egg White peptone:1.0%;NaCl:0.5%;Agar:1.5%~2.0%.
  3. 3. preparation method according to claim 2, it is characterised in that:Also it is added with culture medium described in step 1) (NH4) at least one of 2SO4, extraction from yeast powder, glucose.
  4. 4. preparation method according to claim 1, it is characterised in that:Strain described in step 1) is bacillus, false list At least one of born of the same parents bacterium.
  5. 5. preparation method according to claim 4, it is characterised in that:Strain described in step 1) is bacillus.
  6. 6. preparation method according to claim 1, it is characterised in that:Activated carbon described in step 2) is coconut husk charcoal, wooden At least one of charcoal, coal quality charcoal, fruit shell carbon.
  7. 7. preparation method according to claim 1, it is characterised in that:The shape of activated carbon described in step 2) is honeycomb At least one of shape, column, graininess, powdered.
  8. 8. preparation method according to claim 1, it is characterised in that:The matter of activated carbon, composite bacteria described in step 3) Amount is than being 1: (0.8~1.2).
  9. 9. preparation method according to claim 1, it is characterised in that:Effective viable bacteria in biological activated carbon described in step 3) Content >=2 × 109/gram.
  10. A kind of 10. biological activated carbon for COD of sewage of degrading prepared by the method in claim 1~9 described in any one.
CN201711361278.1A 2017-12-18 2017-12-18 A kind of biological activated carbon and preparation method thereof Withdrawn CN107988200A (en)

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Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109607899A (en) * 2018-12-19 2019-04-12 武汉格林环保设施运营有限责任公司 A kind of green biochemical processing method for reverse osmosis concentrated water
CN109897869A (en) * 2018-08-28 2019-06-18 福建省农业科学院农业工程技术研究所 A kind of high sugared waste water culture using smallfruit fig leaf as the biological activated carbon of carbon source carrier
CN110194533A (en) * 2019-05-31 2019-09-03 江苏竹海活性炭有限公司 Desalination CharcoAid and its preparation process, sewage desalination mode
CN111334382A (en) * 2020-04-17 2020-06-26 江西理工大学 Heavy metal cleaning soap based on spores and preparation method thereof
CN111995063A (en) * 2020-08-18 2020-11-27 普罗生物技术(上海)有限公司 Powdered activated carbon carrier and preparation method and application thereof
CN113185005A (en) * 2021-03-13 2021-07-30 徐韡卿 Method for online regeneration of activated carbon adsorption carrier by biological method
CN115672273A (en) * 2022-10-26 2023-02-03 广东韩研活性炭科技股份有限公司 Biological carrier activated carbon and preparation method thereof

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109897869A (en) * 2018-08-28 2019-06-18 福建省农业科学院农业工程技术研究所 A kind of high sugared waste water culture using smallfruit fig leaf as the biological activated carbon of carbon source carrier
CN109607899A (en) * 2018-12-19 2019-04-12 武汉格林环保设施运营有限责任公司 A kind of green biochemical processing method for reverse osmosis concentrated water
CN109607899B (en) * 2018-12-19 2021-10-15 扬州市博喧环保科技有限公司 Green biochemical treatment method for reverse osmosis concentrated water
CN110194533A (en) * 2019-05-31 2019-09-03 江苏竹海活性炭有限公司 Desalination CharcoAid and its preparation process, sewage desalination mode
CN111334382A (en) * 2020-04-17 2020-06-26 江西理工大学 Heavy metal cleaning soap based on spores and preparation method thereof
CN111995063A (en) * 2020-08-18 2020-11-27 普罗生物技术(上海)有限公司 Powdered activated carbon carrier and preparation method and application thereof
CN113185005A (en) * 2021-03-13 2021-07-30 徐韡卿 Method for online regeneration of activated carbon adsorption carrier by biological method
CN113185005B (en) * 2021-03-13 2023-06-06 徐韡卿 Method for on-line regeneration of activated carbon adsorption carrier by biological method
CN115672273A (en) * 2022-10-26 2023-02-03 广东韩研活性炭科技股份有限公司 Biological carrier activated carbon and preparation method thereof

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Application publication date: 20180504