CN115672273A - Biological carrier activated carbon and preparation method thereof - Google Patents
Biological carrier activated carbon and preparation method thereof Download PDFInfo
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Abstract
The invention relates to a biological carrier activated carbon and a preparation method thereof, belonging to the technical field of efficient activated carbon preparation. The preparation method comprises the steps of selecting peanut shells, straws and corncob cores as active carbon raw materials, inoculating streptomyces albus and streptomyces rubrus to perform pore forming on the active carbon raw materials, then carbonizing AT high temperature to obtain compound active carbon, selecting nitrobacter winogradskyi Y3, nitrosomonas europeae AT7 and denitrifying bacterium Br9 as load strains, and preparing the biological carrier active carbon by combining a sodium alginate gel technology. The biological carrier activated carbon comprises 70-80% of macropores and 10-20% of micropores, the water content is less than 30%, the ash content is less than 20%, the iodine adsorption value is more than 1100mg/g, and the biological carrier activated carbon has high ammonia nitrogen degradation rate and nitrite degradation rate.
Description
Technical Field
The invention belongs to the technical field of preparation of high-efficiency activated carbon, and relates to a biological carrier activated carbon and a preparation method thereof.
Background
The active carbon is a fine carbon particle with a large surface area and fine holes-capillaries inside, so that the active carbon has strong adsorption capacity and can play a role in purifying air or water. The principle of activated carbon for purifying water is that one or more substances in water are adsorbed in pores on the surface of the activated carbon by utilizing the porosity of the activated carbon, and the removed objects comprise organic substances, synthetic detergents, microorganisms, viruses or heavy metals, so that the water is decolorized and deodorized. In addition, many oxides exist on the surface of the activated carbon, most of which exist in the forms of-COOH, -OH, = C = O, and the like, among the oxides, the oxides existing in the forms of-COOH and-OH are acidic in an electrolyte solution, and the oxides existing in the forms of-C = O are alkaline, and if microorganisms are supported on the activated carbon, cell surface proteins of the microorganisms are amphoteric compounds, can react with acid and alkali to form salts, and can be combined through chemical bonding attraction to further purify water bodies, air, and the like.
The metabolism of the microorganism itself can secrete viscous metabolites such as polysaccharides and the like. These in vitro polysaccharides act as biological "glue". So that the microorganisms can be singly clustered around the pores and on the surfaces of the carbon without influencing the physical adsorption effect of the activated carbon. Meanwhile, the existence of the pituitous substances also plays a role in protecting the activated carbon, particularly in the back washing process, the damage caused by friction among the activated carbon is reduced, and the mechanical strength of the activated carbon is indirectly enhanced. Meanwhile, a large amount of organic matters serving as a microbial nutrient source are concentrated in the activated carbon, bacteria can be gathered on the surface of the activated carbon for reproduction, and the activated carbon is a good living place for the bacteria.
However, in the prior art, the raw material of the activated carbon is single, so that the prepared activated carbon has a single pore size and cannot meet the requirement of adsorption and purification of various substances, the activated carbon for purifying water bodies loaded with microorganisms is poor in adsorption performance, and the degradation rate of ammonia nitrogen and nitrite in the water bodies is low due to low microbial activity.
Disclosure of Invention
The invention aims to provide a biological carrier activated carbon and a preparation method thereof, belonging to the technical field of efficient activated carbon preparation. According to the invention, peanut shells, straws and corncobs are selected as raw materials of activated carbon, streptomyces albus and streptomyces rubrus are inoculated to perform pore-forming on the raw materials of the activated carbon, then high-temperature carbonization is performed to obtain compound activated carbon, nitrobacter winogradskyi Y3, nitrosomonas europaea AT7 and denitrifying bacterium Br9 are selected as load strains, and meanwhile, sodium alginate gel technology is combined to prepare the biological carrier activated carbon. The biological carrier activated carbon comprises 70-80% of macropores and 10-20% of micropores, the water content is less than 30%, the ash content is less than 20%, the iodine adsorption value is greater than 1100mg/g, and the biological carrier activated carbon has high ammonia nitrogen degradation rate and nitrite degradation rate.
The purpose of the invention can be realized by the following technical scheme:
a preparation method of biological carrier activated carbon comprises the following steps:
(1) Taking dried peanut shells, straws and corn cobs as raw materials, respectively crushing to obtain peanut shell powder, straw powder and corn cob core powder, mixing the peanut shell powder, the straw powder and the corn cob core powder, and then sterilizing to obtain a composite raw material;
(2) Mixing the liquid culture medium and the composite raw material to obtain a mixed raw material, respectively inoculating streptomyces albus and streptomyces rubrus into the mixed raw material, culturing for 20-30 days at 25-26 ℃, taking out the mixed raw material, drying, and carbonizing at high temperature to obtain compound activated carbon;
(3) Washing the compound activated carbon with deionized water, drying, putting into a hydrochloric acid solution with the mass concentration of 5-10%, controlling the temperature at 60-80 ℃ and keeping the temperature for 1-2h, filtering, washing and drying to obtain pretreated compound activated carbon, then putting into a sodium alginate solution with the mass concentration of 1-2%, adding a compound bacterial liquid containing compound microorganisms, then dropwise adding a calcium chloride solution with the mass concentration of 3-6%, and standing for 30-50min to obtain a mixed liquid containing solid particles;
(4) And filtering the mixed solution, taking filter residues, washing with deionized water, and then airing to obtain the biological carrier activated carbon.
As a preferable technical scheme of the invention, the particle size of the pulverized peanut shell powder, straw powder and corn cob core powder in the step (1) is 55-85um.
The preferable technical scheme of the invention is characterized in that the mass ratio of the peanut shell powder, the straw powder and the corn cob core powder in the step (1) is 1-2:2-3:5-8.
As a preferred technical scheme of the invention, the liquid culture medium in the step (2) comprises 10g/L of peptone, 5g/L of yeast extract, 10g/L of sodium chloride and the balance of deionized water.
As a preferred technical scheme of the invention, the weight ratio of the liquid culture medium and the composite raw material in the step (2) is 2-3:5-9.
As a preferred technical scheme of the invention, the inoculation amount of the streptomyces albus in the step (2) is 1 x 10 6 -2×10 6 cells/g, streptomyces rubiginis inoculum size 5X 10 6 -8×10 6 cells/g。
As a preferred technical scheme of the invention, the high-temperature carbonization in the step (2) is carbonization for 1-2h at 500-600 ℃.
As a preferable technical scheme of the invention, the compound bacterial liquid in the step (3) contains 1 × 10 9 -2×10 9 cells/ml of nitrifying bacteria Vickers Y3, 4X 10 10 -8×10 10 cells/ml of Nitrosomonas europaea AT7, 3X 10 9 -7×10 9 cells/ml denitrifying bacteria Br9.
As a preferred technical scheme of the invention, the mass ratio of the compound activated carbon pretreated in the step (3), the sodium alginate solution, the compound bacteria solution and the calcium chloride solution is 10-15.
The biological carrier activated carbon prepared by the preparation method.
The invention has the beneficial effects that:
(1) According to the invention, peanut shells, straws and corncobs are selected and mixed according to a specific ratio to serve as active carbon raw materials, microbial pore-forming and high-temperature carbonization are combined, and the obtained compound active carbon has a wide pore size distribution range, contains 70-80% of macropores (with the pore size of 50-100 nm) and 10-20% of micropores (with the pore size of 5-20 nm), and has better practicability;
(2) The invention selects the streptomyces albus and the streptomyces griseus to be inoculated in the composite raw material, the streptomyces albus and the streptomyces rubrus are cooperated to decompose the fiber of the composite raw material to a certain degree in a proper temperature range according to a specific proportion, namely, the pore is formed, the adsorptivity of the carbonized compound activated carbon is high, and the iodine adsorption value is more than 1100mg/g;
(3) The invention selects the nitrobacteria Y3, the nitrosomonas europaea AT7 and the denitrifying bacteria Br9 as the load strains, and simultaneously combines the sodium alginate gel technology to prepare the biological carrier activated carbon, the sodium alginate gel enhances the stability between the load strains and the activated carbon, reduces the loss of the strains, simultaneously the sodium alginate provides nutrition for the load strains, and further enhances the activity of the strains, so that the biological carrier activated carbon has higher ammonia nitrogen degradation rate and nitrite degradation rate.
Detailed Description
To further illustrate the technical means and effects of the present invention adopted to achieve the predetermined objects, the following detailed description of the embodiments, structures, features and effects according to the present invention will be provided in conjunction with the embodiments.
Example 1
A preparation method of biological carrier activated carbon comprises the following steps:
(1) Taking dried peanut shells, straws and corn cobs as raw materials, respectively crushing the raw materials to obtain peanut shell powder, straw powder and corn cob powder with the particle size of 60-75um, mixing the peanut shell powder, the straw powder and the corn cob powder according to the mass ratio of 1;
(2) Mixing a liquid culture medium containing 10g/L of peptone, 5g/L of yeast extract, 10g/L of sodium chloride and the balance of deionized water with the composite raw material according to a weight ratio of 3:8 to obtain a mixed raw material, and then respectively inoculating 1 × 10 inoculation amount of streptomyces albus and 1 × 10 inoculation amount of streptomyces rubrus into the mixed raw material 6 cells/g, 6X 10 Streptomyces rubrus inoculation amount 6 cells/g, culturing for 28 days at 25 ℃, taking out and drying the mixed raw materials, and carbonizing at 550 ℃ for 1.5 hours to obtain the compound activated carbon；
(3) Washing the compound activated carbon with deionized water, drying, putting into a hydrochloric acid solution with the mass concentration of 8%, controlling the temperature at 65 ℃ and keeping the temperature for 2 hours, filtering, washing and drying to obtain the pretreated compound activated carbon, putting 12 parts by weight of the pretreated compound activated carbon into 60 parts by weight of a sodium alginate solution with the mass concentration of 2%, and adding 86 parts by weight of sodium alginate solution containing 1 multiplied by 10 9 cells/ml of nitrifying bacteria Vickers Y3, 5X 10 10 cells/ml of Nitrosomonas europaea AT7, 4X 10 9 Adding 67 parts by weight of calcium chloride solution with the mass concentration of 5% into cell/ml composite bacterial liquid of denitrifying bacteria Br9, and standing for 35min to obtain mixed liquid containing solid particles;
(4) And filtering the mixed solution, taking filter residues, washing with deionized water, and then airing to obtain the biological carrier activated carbon.
Example 2
A preparation method of biological carrier activated carbon comprises the following steps:
(1) Taking dried peanut shells, straws and corn cobs as raw materials, respectively crushing the raw materials to the particle size of 65-80um to obtain peanut shell powder, straw powder and corn cob core powder, mixing the peanut shell powder, the straw powder and the corn cob core powder according to the mass ratio of 2;
(2) Mixing a liquid culture medium containing 10g/L of peptone, 5g/L of yeast extract, 10g/L of sodium chloride and the balance of deionized water with the composite raw material according to a weight ratio of 3:7 to obtain a mixed raw material, and then respectively inoculating 1.2 x 10 of streptomyces albus and 1.2 x 10 of streptomyces rubrus into the mixed raw material 6 cells/g, the inoculation amount of streptomyces erythreus is 5 multiplied by 10 6 cells/g, culturing for 25 days at 25.5 ℃, taking out and drying the mixed raw materials, and carbonizing for 1h at 600 ℃ to obtain compound activated carbon;
(3) Washing the compound activated carbon with deionized water, drying, putting into a hydrochloric acid solution with the mass concentration of 5%, controlling the temperature at 72 ℃ and keeping the temperature for 2 hours, filtering, washing and drying to obtain the pretreated compound activated carbon, and then taking 12 parts by weight of the pretreated compound activated carbonAdding activated carbon into 66 parts by weight of sodium alginate solution with mass concentration of 2%, and adding 80 parts by weight of sodium alginate solution containing 1 × 10 9 cells/ml of Nitrobacter vickers Y3, 7X 10 10 cells/ml of Nitrosomonas europaea AT7, 5X 10 9 Adding 80 parts by weight of 3% calcium chloride solution dropwise into cell/ml composite bacterial liquid of denitrifying bacteria Br9, and standing for 45min to obtain mixed liquid containing solid particles;
(4) And filtering the mixed solution, taking filter residues, washing with deionized water, and then airing to obtain the biological carrier activated carbon.
Example 3
A preparation method of biological carrier activated carbon comprises the following steps:
(1) Taking dried peanut shells, straws and corn cobs as raw materials, respectively crushing the raw materials to obtain peanut shell powder, straw powder and corn cob powder with the particle size of 70-80um, mixing the peanut shell powder, the straw powder and the corn cob powder according to the mass ratio of 1;
(2) Mixing a liquid culture medium containing 10g/L of peptone, 5g/L of yeast extract, 10g/L of sodium chloride and the balance of deionized water with the composite raw material according to a weight ratio of 2:9 to obtain a mixed raw material, and then respectively inoculating 2 x 10 inoculation amount of streptomyces albus and 2 x 10 inoculation amount of streptomyces rubrus into the mixed raw material 6 cells/g, streptomyces rubiginis inoculum size 5X 10 6 cells/g, culturing for 21 days at 26 ℃, taking out and drying the mixed raw materials, and carbonizing for 2 hours at the high temperature of 500 ℃ to obtain compound activated carbon;
(3) Washing the compound activated carbon with deionized water, drying, putting into a hydrochloric acid solution with the mass concentration of 10%, controlling the temperature at 70 ℃ and keeping the temperature for 2 hours, filtering, washing and drying to obtain the pretreated compound activated carbon, putting 15 parts by weight of the pretreated compound activated carbon into 72 parts by weight of a sodium alginate solution with the mass concentration of 1.5%, and adding 90 parts by weight of the sodium alginate solution containing 1 multiplied by 10 9 cells/ml of nitrobacteria Vickers Y3, 8X 10 10 cells/ml of Nitrosomonas europaea AT7, 4X 10 9 complexing of denitrifying bacteria Br9 in cells/mlMixing bacterial liquid, then dropwise adding 80 parts by weight of calcium chloride solution with the mass concentration of 6%, and standing for 35min to obtain mixed liquid containing solid particles;
(4) And filtering the mixed solution, taking filter residues, washing with deionized water, and then airing to obtain the biological carrier activated carbon.
Comparative example 1
A preparation method of biological carrier activated carbon comprises the following steps:
(1) Taking dried straws as raw materials, crushing the straws to obtain straw powder with the particle size of 70-80um, and sterilizing the straw powder to obtain an active carbon raw material;
(2) Mixing a liquid culture medium containing 10g/L of peptone, 5g/L of yeast extract, 10g/L of sodium chloride and the balance of deionized water with an activated carbon raw material according to a weight ratio of 2:9 to obtain a mixed raw material, and then respectively inoculating 2 x 10 inoculation amount of streptomyces albus and 2 x 10 inoculation amount of streptomyces rubrus into the mixed raw material 6 cells/g, the inoculation amount of streptomyces erythreus is 5 multiplied by 10 6 cells/g, culturing for 21 days at 26 ℃, taking out and drying the mixed raw materials, and carbonizing for 2 hours at 500 ℃ to obtain straw activated carbon;
(3) Washing straw activated carbon with deionized water, drying, putting into 10% hydrochloric acid solution, controlling temperature at 70 ℃ and keeping the temperature for 2h, filtering, washing and drying to obtain pretreated straw activated carbon, putting 15 parts by weight of the pretreated straw activated carbon into 72 parts by weight of 1.5% sodium alginate solution, and adding 90 parts by weight of 1 × 10 sodium alginate solution 9 cells/ml of nitrobacteria Vickers Y3, 8X 10 10 cells/ml of Nitrosomonas europaea AT7, 4X 10 9 Adding 80 parts by weight of calcium chloride solution with the mass concentration of 6% into cell/ml of composite bacterial liquid of denitrifying bacteria Br9, and standing for 35min to obtain mixed liquid containing solid particles;
(4) And filtering the mixed solution, taking filter residues, washing with deionized water, and airing to obtain the biological carrier activated carbon.
Comparative example 2
A preparation method of biological carrier activated carbon comprises the following steps:
(1) Taking dried peanut shells as a raw material, crushing the raw material to obtain peanut shell powder with the particle size of 70-80um, and sterilizing the peanut shell powder to obtain an activated carbon raw material;
(2) Mixing a liquid culture medium containing 10g/L of peptone, 5g/L of yeast extract, 10g/L of sodium chloride and the balance of deionized water with an activated carbon raw material according to a weight ratio of 2:9 to obtain a mixed raw material, and then respectively inoculating 2 x 10 inoculation amount of streptomyces albus and 2 x 10 inoculation amount of streptomyces rubrus into the mixed raw material 6 cells/g, the inoculation amount of streptomyces erythreus is 5 multiplied by 10 6 cells/g, culturing for 21 days at 26 ℃, taking out and drying the mixed raw materials, and carbonizing for 2 hours at the high temperature of 500 ℃ to obtain the peanut shell activated carbon;
(3) Washing peanut shell activated carbon with deionized water, drying, putting into a hydrochloric acid solution with the mass concentration of 10%, controlling the temperature at 70 ℃ and keeping the temperature for 2 hours, filtering, washing and drying to obtain pretreated peanut shell activated carbon, putting 15 parts by weight of the pretreated peanut shell activated carbon into 72 parts by weight of sodium alginate solution with the mass concentration of 1.5%, and adding 90 parts by weight of sodium alginate solution containing 1 multiplied by 10 9 cells/ml of nitrobacteria Vickers Y3, 8X 10 10 cells/ml Nitrosomonas europea AT7, 4X 10 9 Adding 80 parts by weight of calcium chloride solution with the mass concentration of 6% into cell/ml of composite bacterial liquid of denitrifying bacteria Br9, and standing for 35min to obtain mixed liquid containing solid particles;
(4) And filtering the mixed solution, taking filter residues, washing with deionized water, and airing to obtain the biological carrier activated carbon.
Comparative example 3
A preparation method of biological carrier activated carbon comprises the following steps:
(1) Taking dried corncobs as raw materials, crushing the corncobs to obtain corncob powder with the particle size of 70-80um, and sterilizing the corncob powder to obtain an activated carbon raw material;
(2) Mixing a liquid culture medium containing 10g/L peptone, 5g/L yeast extract, 10g/L sodium chloride and the balance deionized water withMixing the activated carbon raw materials according to the weight ratio of 2:9 to obtain a mixed raw material, and then respectively inoculating streptomyces albus and streptomyces rubrus into the mixed raw material, wherein the inoculation amount of the streptomyces albus is 2 multiplied by 10 6 cells/g, the inoculation amount of streptomyces erythreus is 5 multiplied by 10 6 cells/g, culturing for 21 days at 26 ℃, taking out and drying the mixed raw materials, and carbonizing for 2 hours at a high temperature of 500 ℃ to obtain the corn cob core activated carbon;
(3) Washing the corncob core activated carbon with deionized water, drying, putting into a hydrochloric acid solution with the mass concentration of 10%, controlling the temperature at 70 ℃ and keeping the temperature for 2 hours, filtering, washing and drying to obtain the pretreated corncob core activated carbon, putting 15 parts by weight of the pretreated corncob core activated carbon into 72 parts by weight of sodium alginate solution with the mass concentration of 1.5%, and adding 90 parts by weight of sodium alginate solution containing 1 multiplied by 10 9 cells/ml of nitrobacteria Vickers Y3, 8X 10 10 cells/ml of Nitrosomonas europaea AT7, 4X 10 9 Adding 80 parts by weight of calcium chloride solution with the mass concentration of 6% into cell/ml composite bacterial liquid of denitrifying bacteria Br9, and standing for 35min to obtain mixed liquid containing solid particles;
(4) Filtering the mixed solution, taking filter residue, washing with deionized water, and air drying to obtain the biological carrier activated carbon
Comparative example 4
A preparation method of biological carrier activated carbon comprises the following steps:
(1) Taking dried peanut shells, straws and corn cobs as raw materials, respectively crushing the raw materials to obtain peanut shell powder, straw powder and corn cob powder with the particle size of 70-80um, mixing the peanut shell powder, the straw powder and the corn cob powder according to the mass ratio of 1;
(2) Carbonizing the composite raw material at the high temperature of 500 ℃ for 2 hours to obtain compound activated carbon;
(3) Washing the compound activated carbon with deionized water, drying, putting into a hydrochloric acid solution with the mass concentration of 10%, controlling the temperature at 70 ℃ and keeping the temperature for 2 hours, filtering, washing and drying to obtain pretreated peanut shell activated carbon, and putting 15 parts by weight of the pretreated compound activated carbon into a container with the weight of 72 partsAdding 90 parts by weight of 1X 10 sodium alginate solution with mass concentration of 1.5 percent 9 cells/ml of nitrobacteria Vickers Y3, 8X 10 10 cells/ml of Nitrosomonas europaea AT7, 4X 10 9 Adding 80 parts by weight of calcium chloride solution with the mass concentration of 6% into cell/ml of composite bacterial liquid of denitrifying bacteria Br9, and standing for 35min to obtain mixed liquid containing solid particles;
(4) And filtering the mixed solution, taking filter residues, washing with deionized water, and then airing to obtain the biological carrier activated carbon.
Comparative example 5
A preparation method of biological carrier activated carbon comprises the following steps:
(1) Taking dried peanut shells, straws and corn cobs as raw materials, respectively crushing the raw materials to obtain peanut shell powder, straw powder and corn cob powder with the particle size of 70-80um, mixing the peanut shell powder, the straw powder and the corn cob powder according to the mass ratio of 1;
(2) Mixing a liquid culture medium containing 10g/L of peptone, 5g/L of yeast extract, 10g/L of sodium chloride and the balance of deionized water with the composite raw material according to a weight ratio of 2:9 to obtain a mixed raw material, and then respectively inoculating 5 x 10 inoculation amount of streptomyces albus and 5 x 10 inoculation amount of streptomyces rubrus into the mixed raw material 6 cells/g, streptomyces rubiginis inoculum size 1X 10 6 cells/g, culturing for 21 days at 26 ℃, taking out and drying the mixed raw materials, and carbonizing for 2 hours at the high temperature of 500 ℃ to obtain compound activated carbon;
(3) Washing the compound activated carbon with deionized water, drying, putting into a hydrochloric acid solution with the mass concentration of 10%, controlling the temperature at 70 ℃ and keeping the temperature for 2 hours, filtering, washing and drying to obtain the pretreated compound activated carbon, putting 15 parts by weight of the pretreated compound activated carbon into 72 parts by weight of a sodium alginate solution with the mass concentration of 1.5%, and adding 90 parts by weight of the sodium alginate solution containing 1 multiplied by 10 9 cells/ml of nitrobacteria Vickers Y3, 8X 10 10 cells/ml Nitrosomonas europea AT7, 4X 10 9 cells/ml of denitrifying bacteria Br9 composite bacterial liquid, and then 8 drops of the bacterial liquid0 part by weight of calcium chloride solution with the mass concentration of 6 percent is kept stand for 35min to obtain mixed solution containing solid particles;
(4) And filtering the mixed solution, taking filter residues, washing with deionized water, and then airing to obtain the biological carrier activated carbon.
Comparative example 6
A preparation method of biological carrier activated carbon comprises the following steps:
(1) Taking dried peanut shells, straws and corn cobs as raw materials, respectively crushing the raw materials to obtain peanut shell powder, straw powder and corn cob powder with the particle size of 70-80um, mixing the peanut shell powder, the straw powder and the corn cob powder according to the mass ratio of 1;
(2) Mixing a liquid culture medium containing 10g/L of peptone, 5g/L of yeast extract, 10g/L of sodium chloride and the balance of deionized water with the composite raw material according to a weight ratio of 2:9 to obtain a mixed raw material, and then respectively inoculating 2 x 10 inoculation amount of streptomyces albus and 2 x 10 inoculation amount of streptomyces rubrus into the mixed raw material 6 cells/g, the inoculation amount of streptomyces erythreus is 5 multiplied by 10 6 cells/g, culturing for 21 days at 26 ℃, taking out and drying the mixed raw materials, and carbonizing for 2 hours at the high temperature of 500 ℃ to obtain compound activated carbon;
(3) Washing the compound activated carbon with deionized water, drying, putting into a hydrochloric acid solution with the mass concentration of 10%, controlling the temperature at 70 ℃ and keeping the temperature for 2 hours, filtering, washing and drying to obtain the pretreated compound activated carbon, and putting 15 parts by weight of the pretreated compound activated carbon into 90 parts by weight of the pretreated compound activated carbon containing 1 multiplied by 10 9 cells/ml of nitrobacteria Vickers Y3, 8X 10 10 cells/ml of Nitrosomonas europaea AT7, 4X 10 9 Standing for 35min in cell/ml composite bacterial liquid of denitrifying bacteria Br9 to obtain mixed liquid containing solid particles;
(4) And filtering the mixed solution, taking filter residues, washing with deionized water, and airing to obtain the biological carrier activated carbon.
Performance test
The bio-carrier activated carbon prepared in examples 1-3 and comparative examples 1-6 was tested for moisture content according to GB/T12496.4-1999, ash content according to GB/T12496.3-1999, and iodine adsorption value according to GB/T12496.8-2015, respectively, and the results are shown in Table 1 below.
The biological carrier activated carbon prepared in the examples 1-3 and the comparative examples 1-6 is respectively used for testing the pore size distribution of the biological carrier activated carbon by adopting a nitrogen adsorption experiment, before the test, a sample is firstly subjected to vacuum pumping pretreatment at the high temperature of 150 ℃ for 3 hours, and then high-purity nitrogen with the purity of more than 99.999 percent is used as adsorbate, and the nitrogen adsorption capacity under different relative pressures is measured at the temperature of-195.8 ℃. And drawing a nitrogen adsorption and desorption isotherm by taking the relative pressure as an abscissa and the adsorption quantity of the unit sample mass as an ordinate. The desorption branch of the nitrogen adsorption isotherm is calculated by adopting a BJH method to obtain the pore size distribution of the sample, and the detection result is shown in the following table 1.
50kg of sewage of the Danshan river in the area of the Panyu wine in Guangzhou city is collected, 80g of the biological carrier activated carbon prepared in the examples 1-3 and the comparative examples 1-6 is suspended in the sewage and is kept stand for 12 days at the temperature of 20 ℃, then the concentrations of ammonia nitrogen and nitrite in the sewage before and after treatment are respectively tested by a Nashi reagent photometry and a colorimetry, and the degradation rate is calculated, and the results are shown in the following table 1.
TABLE 1
As can be seen from the test results in Table 1, in comparative example 1, only straw is used as an activated carbon raw material on the basis of example 3, only peanut shells are used as an activated carbon raw material on the basis of example 3, and only corn cob cores are used as an activated carbon raw material on the basis of example 3, the proportion of macropores with the aperture of 50-100nm in the obtained biological carrier activated carbon is obviously reduced, and the iodine adsorption value, the ammonia nitrogen degradation rate and the nitrite degradation rate are also reduced; comparative example 4 no microbial pore-forming is performed on the basis of example 3, the proportion of macropores with the aperture of 50-100nm in the obtained biological carrier activated carbon is obviously reduced, and the iodine adsorption value, the ammonia nitrogen degradation rate and the nitrite degradation rate are also obviously reduced; comparative example 5 the proportion of streptomyces albus and streptomyces rubrus is changed on the basis of example 3, the proportion of macropores with the aperture of 50-100nm in the obtained biological carrier activated carbon is reduced, and the iodine adsorption value, the ammonia nitrogen degradation rate and the nitrite degradation rate are also reduced; comparative example 6 no sodium alginate gelling step was performed on the basis of example 3, and the ammonia nitrogen degradation rate and nitrite degradation rate of the obtained bio-carrier activated carbon were significantly reduced.
Although the present invention has been described with reference to the preferred embodiments, it will be understood by those skilled in the art that various changes may be made and equivalents may be substituted for elements thereof without departing from the scope of the present invention.
Claims (10)
1. The preparation method of the biological carrier activated carbon is characterized by comprising the following steps:
(1) Taking dried peanut shells, straw and corn cobs as raw materials, respectively crushing to obtain peanut shell powder, straw powder and corn cob core powder, mixing the peanut shell powder, the straw powder and the corn cob core powder, and then sterilizing to obtain a composite raw material;
(2) Mixing a liquid culture medium and a composite raw material to obtain a mixed raw material, respectively inoculating streptomyces albus and streptomyces rubrus into the mixed raw material, culturing for 20-30 days at 25-26 ℃, taking out the mixed raw material, drying, and carbonizing at high temperature to obtain compound activated carbon;
(3) Washing the compound activated carbon with deionized water, drying, putting into a hydrochloric acid solution with the mass concentration of 5-10%, controlling the temperature at 60-80 ℃ and keeping the temperature for 1-2h, filtering, washing and drying to obtain pretreated compound activated carbon, then putting into a sodium alginate solution with the mass concentration of 1-2%, adding a compound bacterial liquid containing compound microorganisms, then dropwise adding a calcium chloride solution with the mass concentration of 3-6%, and standing for 30-50min to obtain a mixed liquid containing solid particles;
(4) And filtering the mixed solution, taking filter residues, washing with deionized water, and then airing to obtain the biological carrier activated carbon.
2. The method for preparing biological carrier activated carbon as claimed in claim 1, wherein the particle size of the pulverized peanut shell powder, straw powder and corn cob core powder in step (1) is 55-85um.
3. The preparation method of the biological carrier activated carbon as claimed in claim 1, wherein the mass ratio of the mixture of the peanut shell powder, the straw powder and the corncob core powder in the step (1) is 1-2:2-3:5-8.
4. The method for preparing a bio-carrier activated carbon as claimed in claim 1, wherein the liquid medium in step (2) comprises 10g/L of peptone, 5g/L of yeast extract, 10g/L of sodium chloride and the balance of deionized water.
5. The method for preparing the biological carrier activated carbon as claimed in claim 1, wherein the weight ratio of the liquid culture medium to the composite raw material in the step (2) is 2-3:5-9.
6. The method for preparing the biological carrier activated carbon according to claim 1, wherein the inoculation amount of the streptomyces albus in the step (2) is 1 x 10 6 -2×10 6 cells/g, streptomyces rubiginis inoculum size 5X 10 6 -8×10 6 cells/g。
7. The method for preparing the biological carrier activated carbon according to claim 1, wherein the high-temperature carbonization in the step (2) is performed at 500-600 ℃ for 1-2h.
8. The method for preparing biological carrier activated carbon according to claim 1, wherein the composite bacterial liquid in the step (3) contains 1 x 10 9 -2×10 9 cells/ml of nitrifying bacteria Vickers Y3, 4X 10 10 -8×10 10 cells/ml of Nitrosomonas europaea AT7, 3X 10 9 -7×10 9 cells/ml denitrifying bacteria Br9.
9. The preparation method of the biological carrier activated carbon according to claim 1, wherein the mass ratio of the pretreated compound activated carbon in the step (3) to the sodium alginate solution to the compound bacteria solution to the calcium chloride solution is 10-15.
10. A bio-carrier activated carbon prepared by the preparation method of any one of claims 1 to 9.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN202211339814.9A CN115672273B (en) | 2022-10-26 | 2022-10-26 | Biological carrier activated carbon and preparation method thereof |
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