CN110280126A - It is a kind of to purify air combined biologically active agents and preparation method thereof - Google Patents
It is a kind of to purify air combined biologically active agents and preparation method thereof Download PDFInfo
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Abstract
The invention belongs to Techniques for Indoor Air Purification fields, more particularly to a kind of air combined biologically active agents of purification and preparation method thereof, the problem of solved, is to provide air combined biologically active agents of a kind of purification and preparation method thereof, its air purifying preparation obtained can be by biologically active microorganism, organized enzyme, plant extracts and plant polyose are all combined preparation, and purification of the activity completion to air pollutants between each component can be given full play to, a kind of technical solution of use are as follows: air combined biologically active agents of purification, each component is according to following weight: 5-10 parts of microbial inoculum, 8-20 parts of organized enzyme, 0.5-2 parts of plant polyose, 20-45 parts of traditional Chinese medicine extraction mixed liquor, 1-8 parts of chitin, 45-75 parts of deionized water, the present invention is widely used in room air Purification techniques field.
Description
Technical field
The invention belongs to Techniques for Indoor Air Purification fields, and in particular to a kind of air combined biologically active agents of purification and
Preparation method.
Background technique
The major pollutants of room air are volatile organic matters, mainly from building and house decorative material, cook with
The combustion product of smoking and other indoor activities of people.Volatile organic compounds has 350 kinds or more, existing biological dirt
Object is contaminated, also there is contaminants of chemical origin, there are also radioactive pollutants, it has the characteristics of diversity, accumulation property and chronicity.It is indoor
Environment is the space of relative closure, these pollutants can be built up indoors, and concentration is caused to increase, and constitutes the danger to human body
Evil.People's most of the time is in interior,, also can be to human body in long term after human body even if the pollutant that concentration is very low
Health has an adverse effect.According to statistics, the time of 80% or more modern is spent indoors, and infant, the old,weak,sick and disabled person are in room
The interior time is longer.Therefore, for the air overwhelming majority of people's breathing from interior, indoor air quality directly affects the strong of people
Health.It is reported that room air pollution has caused 35.7% respiratory disease, 22% chronic lung disease and 15% tracheae
Inflammation, bronchitis and lung cancer.So room air pollution have become to public health endanger maximum five kinds of environmental factors it
One.
Current purification method most common on the market is using active carbon, photocatalyst technology, plant purification agent and biology drop
Solution etc..When active carbon purifying air is increased to a certain degree with temperature, wind speed, adsorbed pollutant is possible to free
Out, it is again introduced into breathing space；And activated carbon adsorption easily reaches adsorption saturation, needs by being activated ability
Secondary use.Photocatalyst is a kind of using Nano titanium dioxide as the total of the photosemiconductor material with photo-catalysis function of representative
Claim, titanium dioxide is nontoxic, but Nano titanium dioxide directly can enter blood circulation by lung, and photocatalyst is not
Formaldehyde can be removed for a long time, be easy to fall off, it cannot depollution object again after falling off.Plant purification agent is having in general extraction plant
Effect ingredient is configured to plant source thimerosal, sprays into air and plays the role of depollution object, but its raw material generally requires many kinds,
Biggish workshop storage raw material is needed, extraction efficiency is low, if the improper product for also resulting in extraction of extraction conditions cannot be sent out
Wave the effect of depollution object.Biodegrade is to utilize the compound bio group for passing through screening and culture and biological enzyme by formaldehyde, benzene, first
The pernicious gases such as benzene, dimethylbenzene, ammonia, TVOC resolve into carbon dioxide and water, to achieve the purpose that the improvement to air.The party
Method is high-efficient, is a kind of more long-acting, safety removal air pollution without side-effects solution without secondary pollution.
But widely used air purifying preparation currently on the market is mostly to realize purification Interior Space using single purification method
The purpose of gas, there is no be combined above-mentioned biologically active microorganism, organized enzyme, plant extracts and plant polyose
The product of composite bio-active air purifying preparation.
Summary of the invention
The invention overcomes the deficiencies of the prior art, and provides a kind of air combined biologically active agents of purification and its
Preparation method, air purifying preparation obtained can be by biologically active microorganism, organized enzyme, plant extracts and plants
Polysaccharide is all combined preparation, and can give full play to purification of the activity completion to air pollutants between each component, due to
It is pure bioactive, secondary pollution will not be generated and effect can be played for a long time, and is all friendly to human body and environment.
In order to solve the above-mentioned technical problem, a kind of the technical solution adopted by the present invention are as follows: air combined bioactivity of purification
Preparation, each component is according to following weight: 5-10 parts of microbial inoculum, 8-20 parts of organized enzyme, and 0.5-2 parts of plant polyose,
20-45 parts of traditional Chinese medicine extraction mixed liquor, 1-8 parts of chitin, 45-75 parts of deionized water；
The microbial inoculum is lactic acid bacteria, photosynthetic bacteria, saccharomycete, bacillus subtilis, pseudomonad and rhizopus；
The organized enzyme is lysozyme, glucose oxidase, lignin peroxidase, protease, lipase and Lumbrokinase；
The plant polyose is polysaccharides from ginkgo biloba, pheophytin and sea grass polysaccharide；
The traditional Chinese medicine extraction mixed liquor is the extract mixed liquor of Radix Isatidis, honeysuckle, purslane, cordate houttuynia and radix scutellariae.
The mixed volume ratio of each bacterium solution of microbial inoculum is lactic acid bacteria: photosynthetic bacteria: saccharomycete: bacillus subtilis
Bacterium: pseudomonad: rhizopus=1-5:1-2:1-2:1-3:1-2:0.5-1.
The weight of the organized enzyme each component are as follows: 10-15 parts of lysozyme, 5-10 parts of glucose oxidase, wooden
Plain peroxidase 1-5 parts, 10-25 parts of protease, 12-20 parts of lipase, 1-5 parts of Lumbrokinase.
The plant polyose each group distribution ratio is polysaccharides from ginkgo biloba 3-8 parts, 0.1-0.5 parts of pheophytin, 5-10 parts of sea grass polysaccharide.
The traditional Chinese medicine extraction mixed liquor is Radix Isatidis, honeysuckle, purslane, cordate houttuynia and radix scutellariae extracting solution according to parts by weight
1:1:1:1:1 is mixed to prepare.
The chitin molecule amount is 7000-10000.
A kind of air combined biologically active agents preparation method of purification, follows the steps below:
1) microbial inoculum is produced
After the activation of lactic acid bacteria, photosynthetic bacteria, saccharomycete, bacillus subtilis, pseudomonad and rhizopus, it is inoculated in ox respectively
In meat extract peptone culture medium, in 37 DEG C, shaking table culture for 24 hours, lactobacillus solution, photosynthetic bacteria seed liquor, saccharomycete is made
Seed liquor, bacillus subtilis seed liquor, pseudomonad seed liquor and rhizopus seed liquor；
By lactobacillus solution, photosynthetic bacteria seed liquor, saccharomycete seed liquor, bacillus subtilis seed liquor, pseudomonad kind
Sub- liquid and the mixing of rhizopus seed liquor, are inoculated in mixing fermentation culture for mixed bacteria liquid with the inoculum concentration of percent by volume 8-15%
In base, shaking table culture 2-3d under the conditions of 37 DEG C, 180r/min；
Then bacterium solution 3000-5000r/min culture obtained is centrifuged 5-8min, takes supernatant to get microbial inoculum is arrived；
2) traditional Chinese medicine extraction mixed liquor
The Radix Isatidis, honeysuckle, purslane, cordate houttuynia and radix scutellariae are weighed by weight, and each medicinal material extracts extracting solution respectively, will
Pulverizing medicinal materials are mixed with its 3-6 times water, are then successively digested through cellulase, pectase, protease；Hydrolysis temperature is
25-35 DEG C, the time is respectively 1-3h；It is then centrifuged for isolated supernatant, Chinese medicine is obtained after the supernatant of each medicinal material is mixed and mentions
Take mixed liquor；
3) plant polyose and organized enzyme
The lysozyme, glucose oxidase, lignin peroxidase, protease, lipase, Lumbrokinase are weighed, ginkgo is more
Sugar, pheophytin and sea grass polysaccharide pass through ultrafiltration membrance filter with the deionized water mixed dissolution under the conditions of 0.1-0.3MPa,
Impurity is removed, 1-3h is stood, obtains plant polyose and active enzyme solutions；
4) solution obtained by step 1) and 2) is mixed according to the solution that parts by weight are obtained with step 3), adds the first
Shell element, is sufficiently mixed and uniformly purifies air active ingredient to get to finished product.
The mixed fermentive culture medium are as follows: glucose 15g/l, soy peptone 4.8g/l, yeast extract 2.5g/l, lemon
Sour ammonium 1.8g/l, sodium acetate 5g/l, K2HPO42g/l, Tween 80 1ml/l, MgSO4·7H2O 0.6g/l, MnSO4·4H2O:
0.3g/l, water constant volume to 1L.
The plant polyose is polysaccharides from ginkgo biloba, pheophytin and sea grass polysaccharide；
The traditional Chinese medicine extraction mixed liquor is the extract mixed liquor of Radix Isatidis, honeysuckle, purslane, cordate houttuynia and radix scutellariae
Pseudomonad (pseudomonadaceae), optional but be not limited to, Pseudomonas fluorescens, pseudomonas aeruginosa, stench are false
One of monad, Pseudomonas stutzeri or more than one.
Saccharomycete (saccharomycetes), optional but be not limited to, brewer's yeast, Hansenula yeast, torulopsis, false silk
One of yeast, Pichia pastoris, rhodotorula, cotton disease needle spore yeast, saccharomyces uvarum bacterium or more than one.
Rhizopus (Rhizopus oryzae), it is optional but be not limited to, it is Rhizopus oryzae, rhizopus chinensis bacterium, Rhizopus a rrhiz, few
One of spore head mold rhizopus stolonifer bacterium, rhizopus niger or more than one.
Lactic acid bacteria (Lactobacillus), it is optional but be not limited to, Bifidobacterium, enterococcus faecium, galactococcus, spherical hammer
One of bacterium, lactobacillus acidophilus, micrococcus or more than one.
Photosynthetic bacteria (Photosynthetic Bacteria) is optional but is not limited to, cyanobacteria, purple bacteria, green bacterium and
One of salt bacterium or more than one.
Lysozyme (lysozyme) is a kind of alkaline enzyme that can be hydrolyzed and stick polysaccharide in pathogenic bacteria.It is main to pass through destruction cell
The β between -acetylmuramic acid and n-acetylglucosamine-Isosorbide-5-Nitrae glycosidic bond in wall decomposes the insoluble glutinous polysaccharide of cell wall
At soluble glycopeptide, cell wall rupture content is caused to escape and make bacterolysis.Lysozyme can also be with negatively charged virus
Albumen is bound directly, and is formed double salt with DNA, RNA, apoprotein, is made virally inactivated.Therefore, the enzyme have antibacterial, anti-inflammatory,
The effects of antiviral.
Glucose oxidase can be catalyzed β-D- glucogenesis gluconic acid and hydrogen peroxide in specific manner under aerobic conditions
Aerobic dehydrogenase is also referred to as oxidizing ferment, such as amine oxidase, xanthine oxidase by people's habit.Glucose oxidase divides extensively
It is distributed in animals and plants and microbial body, is able to suppress peroxide in environment with stronger antioxidation and human body is invaded
Evil.
Protease is widely present in pluck, plant stem-leaf, fruit and microorganism.The present invention is optional but is not limited to,
One of pepsin, trypsase, cathepsin, papain or more than one.
Polysaccharides from ginkgo biloba has anti-oxidant, antitumor, antibacterial, anti-corrosion and other effects.Ginkgo biloba p.e and its preparation are being cured
Cardiovascular and cerebrovascular disease and senile dementia etc. significant effect.Containing with antiallergy, anti-inflammatory, antioxygen in gingko episperm
The ginkgo acids ingredient for the effects of changing, is antibacterial and polysaccharide component with preferable antitumor action.
Pheophytin (also known as fucoidin/algal polysaccharide/Fu is reachable), is a kind of water-soluble dietary fiber, it is thin to be derived from brown alga
The exclusive glutinous sliding ingredient of born of the same parents.Chemically, it is a kind of polysaccharide body based on " sulfate (FUCOSE) ".Pheophytin sheet
Body is a kind of anti-oxidant, decomposition free radical and the natural materials for enhancing immunocompetence, while having very strong bacteriostasis.
Sea grass polysaccharide is mainly by monosaccharide groups such as rhamnose, xylose, glucose (a small amount of mannose, galactolipin, arabinose)
At, and containing uronic acid and sulfate, polysaccharide main chain is containing there are many connecting key types.Sea grass polysaccharide have immunocompetence with it is anti-oxidant
Activity, while there are a variety of effects such as suppression cancer, reducing blood lipid, blood coagulation, moisturizing.
Radix Isatidis, property is bitter, trembles with fear.With clearing heat and detoxicating, the effect of cool blood relieving sore-throat.It is usually used in poison when warm epidemic disease, pharyngalgia of generating heat, temperature
Malicious macular eruption, mumps, scarlet fever, major part pestilence, erysipelas, carbuncle swells.There are clearing heat and detoxicating, antibacterial and anti-inflammation functions.To a variety of grams
Positive bacteria, negative bacterium and influenza virus have inhibiting effect.
Honeysuckle, it is cold in nature, it is sweet in flavor, enter lung, the heart, stomach meridian, has effects that clearing heat and detoxicating, anti-inflammatory, qi-restoratives treats wind, cure mainly swollen
Completely descend disease, warm disease heating, the diseases such as heat toxin large carbuncle and tumour.Detoxify antibacterial action.Effective to various bacteria, infected by influenza also has
Inhibiting effect.
Purslane, property acid, trembles with fear.It is clearing heat and detoxicating, cooling blood and hemostasis.For hot dysentery purulence blood, heat gonorrhea, vaginal discharge diseases, Carbuncle swells dislikes sore, pellet
Poison.With desinsection removing toxic substances, the scattered blood of the heat that disappears and bacteriostasis.
Cordate houttuynia, nature and flavor are pungent, tepor, have the effect of clearing heat and detoxicating, inducing diuresis to remove edema.Control pneumonia, lung abscess, hot dysentery, malaria
Disease, oedema, stranguria syndrome, leukorrhea, carbuncle swells, hemorrhoid, rectal prolapse, eczema, the favus of the scalp, scabies.Antibacterial action is strong, to Diplococcus pneumopniae, golden Portugal
Grape coccus, hemolytic streptococcus, tubercle bacillus effect are most significant.High concentration still inhibits fungi to grow.Also body can be improved to exempt from
Epidemic disease effect.
Radix scutellariae: bitter；It is cold in nature；Return lung；The heart；Liver；Gallbladder；Large intestine channel.Clearing heat-fire；Eliminating dampness removing toxic substances.Main cough with lung heat；Pyreticosis
Unconsciousness due to high fever；Irascibility headache；Red eye, swell pain；Jaundice with damp-heat pathogen；Dysentery；Heat gonorrhea；Hematemesis blood；Metrorrhagia and metrostaxis；Fetal movement with fever；Carbuncle swells furunculosis.
Traditional Chinese medicine extraction mixed liquor is used as compounding using Radix Isatidis, honeysuckle, purslane, cordate houttuynia and radix scutellariae, using enzyme process
Effective biological active matter is extracted, is maximum extraction active material, except having effects that drug matching, there are also active components
The effect of.
Lactic acid bacteria, photosynthetic bacteria, saccharomycete, bacillus subtilis, pseudomonad and rhizopus combine the lysozyme being added,
Glucose oxidase, lignin peroxidase, protease and lipase, under the double action of microorganism and enzyme, by peculiar smell
The organic matter in source is captured and is thoroughly decomposed, and generates carbon dioxide and water, after pernicious gas is reduced, microbiologic population part is dead
It dies, partially enters dormant state, when pernicious gas discharges, microbiologic population is activated breeding, can be for a long time to harmful gas
Body is degraded.
Lumbrokinase is added to as bleeding agent in the present invention simultaneously, is the histone hydrolase extracted from extraordinary earthworm
(acidic protein) can assist in the active material in the present invention with very strong permeability as a kind of antithrombotic agent
Indoor material surface is penetrated further further to degrade to the harmful organic substance of internal layer.
The chitin that the present invention uses wherein is playing the role of carrier and sustained release, and biological active matter and synthetic are isolated
It works, and gives full play to removal formaldehyde in air, benzene class, the purpose of ammonia and other volatile organic matters and sources of odor.
Compared with the prior art, the invention has the beneficial effects that: the present invention is by combining biological enzyme, microorganism, plant
The effect of the multiple degrading of extract and plant polyose is prepared into air purifying preparation.The bioactivity air purifying preparation is made by decomposing
With, the organic matter of formaldehyde in air, benzene, dimethylbenzene, TVOC and other sources of odor can be removed for a long time, also have sterilization function
Effect, whole process is environment-protecting and non-poisonous, does not generate secondary pollution.Beneficial microbe and its metabolite mainly utilize the new of microorganism
Old metabolic activity is converted the substance with peculiar smell, can be with odorants such as fast decoupled ammonia, sulfide, phenols；Formaldehyde,
Benzene, volatile organic matter etc. are used for the substance of synthesized micro-organism itself as the nutriment of degrading microorganism and maintain it normal
The key of the energy needed for vital sign, benzene homologues degradation is the cracking of phenyl ring, and microorganism can be converted into different benzene homologues
The lower key intermediate of stability is later converted key intermediate to by ring-opening reaction in the intermediate of TCA circulation, gone forward side by side
One step recycles the degradation for completing benzene homologues by TCA.When air pollutant concentration is high, it is resistant to degradation of formaldehyde beneficial to micro-
Biological mass propagation, other harmful microorganisms are then dead or generation spore enters dormant period, with the drop of air pollutant concentration
Low, the quantity of degrading microorganism bacterium can also be reduced.In the process, pollutant is converted into microorganism Self substances and required
Nutrition supplying own growth, to completely remove pollution.Simultaneously in biodegradation process, degradation profitable strain plays a role, greatly
Amount breeding, take up space rapidly advantage, becomes dominant population, so that pernicious bacteria living space resource is greatly reduced, harmful bacteria
Quantity is reduced；This product can efficiently remove rapidly artificial board and free formaldehyde, benzene, volatile organic matter in textile article etc.
Pollutant reduces pernicious bacteria quantity, can quick taste removal, eliminate the injury to human body.This product can nontoxic, nothing
It is color, non-corrosive, there are the characteristics such as efficient, lasting, safe and simple, significantly play the role of purifying air.
Specific embodiment
The present invention is further detailed with the following Examples.
Embodiment 1
A kind of air combined biologically active agents of purification, each component is according to following weight: 10 parts of microbial inoculum, activity
20 parts of enzyme, 2 parts of plant polyose, 45 parts of traditional Chinese medicine extraction mixed liquor, 8 parts of chitin, 75 parts of deionized water；
The microbial inoculum is lactic acid bacteria, photosynthetic bacteria, saccharomycete, bacillus subtilis, pseudomonad and rhizopus；
The organized enzyme is lysozyme, glucose oxidase, lignin peroxidase, protease, lipase and Lumbrokinase；
The plant polyose is polysaccharides from ginkgo biloba, pheophytin and sea grass polysaccharide；
The traditional Chinese medicine extraction mixed liquor is the extract mixed liquor of Radix Isatidis, honeysuckle, purslane, cordate houttuynia and radix scutellariae.
The mixed volume ratio of each bacterium solution of microbial inoculum is lactic acid bacteria: photosynthetic bacteria: saccharomycete: bacillus subtilis
Bacterium: pseudomonad: rhizopus=5:2:2:3:2:1.
The weight of the organized enzyme each component are as follows: 15 parts of lysozyme, 10 parts of glucose oxidase, lignin peroxide
5 parts of compound enzyme, 25 parts of protease, 20 parts of lipase, 5 parts of Lumbrokinase.
The plant polyose each group distribution ratio is 8 parts of polysaccharides from ginkgo biloba, 0.5 part of pheophytin, 10 parts of sea grass polysaccharide.
The traditional Chinese medicine extraction mixed liquor is Radix Isatidis, honeysuckle, purslane, cordate houttuynia and radix scutellariae extracting solution according to parts by weight
1:1:1:1:1 is mixed to prepare.
The chitin molecule amount is 7000-10000.
A kind of air combined biologically active agents preparation method of purification, follows the steps below:
1) microbial inoculum is produced
After the activation of lactic acid bacteria, photosynthetic bacteria, saccharomycete, bacillus subtilis, pseudomonad and rhizopus, it is inoculated in ox respectively
In meat extract peptone culture medium, in 37 DEG C, shaking table culture for 24 hours, lactobacillus solution, photosynthetic bacteria seed liquor, saccharomycete is made
Seed liquor, bacillus subtilis seed liquor, pseudomonad seed liquor and rhizopus seed liquor；
By lactobacillus solution, photosynthetic bacteria seed liquor, saccharomycete seed liquor, bacillus subtilis seed liquor, pseudomonad kind
Sub- liquid and the mixing of rhizopus seed liquor, are inoculated in mixed fermentive culture medium for mixed bacteria liquid with the inoculum concentration of percent by volume 15%
In, shaking table culture 2-3d under the conditions of 37 DEG C, 180r/min；
Then bacterium solution 5000r/min culture obtained is centrifuged 5min, takes supernatant to get microbial inoculum is arrived；
2) traditional Chinese medicine extraction mixed liquor
The Radix Isatidis, honeysuckle, purslane, cordate houttuynia and radix scutellariae are weighed by weight, and each medicinal material extracts extracting solution respectively, will
Pulverizing medicinal materials are mixed with its 6 times water, are then successively digested through cellulase, pectase, protease；Hydrolysis temperature is 35
DEG C, the time is respectively 1h；It is then centrifuged for isolated supernatant, traditional Chinese medicine extraction is obtained after the supernatant of each medicinal material is mixed and is mixed
Liquid；
3) plant polyose and organized enzyme
The lysozyme, glucose oxidase, lignin peroxidase, protease, lipase, Lumbrokinase are weighed, ginkgo is more
Sugar, pheophytin and sea grass polysaccharide pass through ultrafiltration membrance filter, removal with the deionized water mixed dissolution under the conditions of 0.3MPa
Impurity stands 3h, obtains plant polyose and active enzyme solutions；
4) solution obtained by step 1) and 2) is mixed according to the solution that parts by weight are obtained with step 3), adds the first
Shell element, is sufficiently mixed and uniformly purifies air active ingredient to get to finished product.
The mixed fermentive culture medium are as follows: glucose 15g/l, soy peptone 4.8g/l, yeast extract 2.5g/l, lemon
Sour ammonium 1.8g/l, sodium acetate 5g/l, K2HPO42g/l, Tween 80 1ml/l, MgSO4·7H2O 0.6g/l, MnSO4·4H2O:
0.3g/l, water constant volume to 1L.
Embodiment 2
A kind of air combined biologically active agents of purification, each component is according to following weight: 5 parts of microbial inoculum, activity
8 parts of enzyme, 0.5 part of plant polyose, 20 parts of traditional Chinese medicine extraction mixed liquor, 1 part of chitin, 45 parts of deionized water；
The microbial inoculum is lactic acid bacteria, photosynthetic bacteria, saccharomycete, bacillus subtilis, pseudomonad and rhizopus；
The organized enzyme is lysozyme, glucose oxidase, lignin peroxidase, protease, lipase and Lumbrokinase；
The plant polyose is polysaccharides from ginkgo biloba, pheophytin and sea grass polysaccharide；
The traditional Chinese medicine extraction mixed liquor is the extract mixed liquor of Radix Isatidis, honeysuckle, purslane, cordate houttuynia and radix scutellariae.
The mixed volume ratio of each bacterium solution of microbial inoculum is lactic acid bacteria: photosynthetic bacteria: saccharomycete: bacillus subtilis
Bacterium: pseudomonad: rhizopus=1:1:1:1:1:0.5.
The weight of the organized enzyme each component are as follows: 10 parts of lysozyme, 5 parts of glucose oxidase, lignin peroxide
1 part of compound enzyme, 10 parts of protease, 12 parts of lipase, 1 part of Lumbrokinase.
The plant polyose each group distribution ratio is 3 parts of polysaccharides from ginkgo biloba, 0.1 part of pheophytin, 5 parts of sea grass polysaccharide.
The traditional Chinese medicine extraction mixed liquor is Radix Isatidis, honeysuckle, purslane, cordate houttuynia and radix scutellariae extracting solution according to parts by weight
1:1:1:1:1 is mixed to prepare.
The chitin molecule amount is 7000-10000.
A kind of air combined biologically active agents preparation method of purification, follows the steps below:
1) microbial inoculum is produced
After the activation of lactic acid bacteria, photosynthetic bacteria, saccharomycete, bacillus subtilis, pseudomonad and rhizopus, it is inoculated in ox respectively
In meat extract peptone culture medium, in 37 DEG C, shaking table culture for 24 hours, lactobacillus solution, photosynthetic bacteria seed liquor, saccharomycete is made
Seed liquor, bacillus subtilis seed liquor, pseudomonad seed liquor and rhizopus seed liquor；
By lactobacillus solution, photosynthetic bacteria seed liquor, saccharomycete seed liquor, bacillus subtilis seed liquor, pseudomonad kind
Sub- liquid and the mixing of rhizopus seed liquor, are inoculated in mixed fermentive culture medium for mixed bacteria liquid with the inoculum concentration of percent by volume 8%
In, shaking table culture 2-3d under the conditions of 37 DEG C, 180r/min；
Then bacterium solution 3000r/min culture obtained is centrifuged 8min, takes supernatant to get microbial inoculum is arrived；
2) traditional Chinese medicine extraction mixed liquor
The Radix Isatidis, honeysuckle, purslane, cordate houttuynia and radix scutellariae are weighed by weight, and each medicinal material extracts extracting solution respectively, will
Pulverizing medicinal materials are mixed with its 3 times water, are then successively digested through cellulase, pectase, protease；Hydrolysis temperature is 25
DEG C, the time is respectively 3h；It is then centrifuged for isolated supernatant, traditional Chinese medicine extraction is obtained after the supernatant of each medicinal material is mixed and is mixed
Liquid；
3) plant polyose and organized enzyme
The lysozyme, glucose oxidase, lignin peroxidase, protease, lipase, Lumbrokinase are weighed, ginkgo is more
Sugar, pheophytin and sea grass polysaccharide pass through ultrafiltration membrance filter, removal with the deionized water mixed dissolution under the conditions of 0.1MPa
Impurity stands 3h, obtains plant polyose and active enzyme solutions；
4) solution obtained by step 1) and 2) is mixed according to the solution that parts by weight are obtained with step 3), adds the first
Shell element, is sufficiently mixed and uniformly purifies air active ingredient to get to finished product.
The mixed fermentive culture medium are as follows: glucose 15g/l, soy peptone 4.8g/l, yeast extract 2.5g/l, lemon
Sour ammonium 1.8g/l, sodium acetate 5g/l, K2HPO42g/l, Tween 80 1ml/l, MgSO4·7H2O 0.6g/l, MnSO4·4H2O:
0.3g/l, water constant volume to 1L.
Embodiment 3
A kind of air combined biologically active agents of purification, each component is according to following weight: 8 parts of microbial inoculum, activity
15 parts of enzyme, 1.4 parts of plant polyose, 33 parts of traditional Chinese medicine extraction mixed liquor, 5 parts of chitin, 66 parts of deionized water；
The microbial inoculum is lactic acid bacteria, photosynthetic bacteria, saccharomycete, bacillus subtilis, pseudomonad and rhizopus；
The organized enzyme is lysozyme, glucose oxidase, lignin peroxidase, protease, lipase and Lumbrokinase；
The plant polyose is polysaccharides from ginkgo biloba, pheophytin and sea grass polysaccharide；
The traditional Chinese medicine extraction mixed liquor is the extract mixed liquor of Radix Isatidis, honeysuckle, purslane, cordate houttuynia and radix scutellariae.
The mixed volume ratio of each bacterium solution of microbial inoculum is lactic acid bacteria: photosynthetic bacteria: saccharomycete: bacillus subtilis
Bacterium: pseudomonad: rhizopus=3:1:1:2.5:2:0.5.
The weight of the organized enzyme each component are as follows: 12 parts of lysozyme, 8 parts of glucose oxidase, lignin peroxide
3 parts of compound enzyme, 18 parts of protease, 15 parts of lipase, 2 parts of Lumbrokinase.
The plant polyose each group distribution ratio is 4 parts of polysaccharides from ginkgo biloba, 0.4 part of pheophytin, 8 parts of sea grass polysaccharide.
The traditional Chinese medicine extraction mixed liquor is Radix Isatidis, honeysuckle, purslane, cordate houttuynia and radix scutellariae extracting solution according to parts by weight
1:1:1:1:1 is mixed to prepare.
The chitin molecule amount is 7000-10000.
A kind of air combined biologically active agents preparation method of purification, follows the steps below:
1) microbial inoculum is produced
After the activation of lactic acid bacteria, photosynthetic bacteria, saccharomycete, bacillus subtilis, pseudomonad and rhizopus, it is inoculated in ox respectively
In meat extract peptone culture medium, in 37 DEG C, shaking table culture for 24 hours, lactobacillus solution, photosynthetic bacteria seed liquor, saccharomycete is made
Seed liquor, bacillus subtilis seed liquor, pseudomonad seed liquor and rhizopus seed liquor；
By lactobacillus solution, photosynthetic bacteria seed liquor, saccharomycete seed liquor, bacillus subtilis seed liquor, pseudomonad kind
Sub- liquid and the mixing of rhizopus seed liquor, are inoculated in mixing fermentation culture for mixed bacteria liquid with the inoculum concentration of percent by volume 8-15%
In base, shaking table culture 2-3d under the conditions of 37 DEG C, 180r/min；
Then bacterium solution 4000r/min culture obtained is centrifuged 6min, takes supernatant to get microbial inoculum is arrived；
2) traditional Chinese medicine extraction mixed liquor
The Radix Isatidis, honeysuckle, purslane, cordate houttuynia and radix scutellariae are weighed by weight, and each medicinal material extracts extracting solution respectively, will
Pulverizing medicinal materials are mixed with its 5 times water, are then successively digested through cellulase, pectase, protease；Hydrolysis temperature is 30
DEG C, the time is respectively 1.5h；It is then centrifuged for isolated supernatant, traditional Chinese medicine extraction is obtained after the supernatant of each medicinal material is mixed and is mixed
Liquid；
3) plant polyose and organized enzyme
The lysozyme, glucose oxidase, lignin peroxidase, protease, lipase, Lumbrokinase are weighed, ginkgo is more
Sugar, pheophytin and sea grass polysaccharide pass through ultrafiltration membrance filter, removal with the deionized water mixed dissolution under the conditions of 0.25MPa
Impurity stands 2h, obtains plant polyose and active enzyme solutions；
4) solution obtained by step 1) and 2) is mixed according to the solution that parts by weight are obtained with step 3), adds the first
Shell element, is sufficiently mixed and uniformly purifies air active ingredient to get to finished product.
The mixed fermentive culture medium are as follows: glucose 15g/l, soy peptone 4.8g/l, yeast extract 2.5g/l, lemon
Sour ammonium 1.8g/l, sodium acetate 5g/l, K2HPO42g/l, Tween 80 1ml/l, MgSO4·7H2O 0.6g/l, MnSO4·4H2O:
0.3g/l, water constant volume to 1L.
The above-mentioned purification air active ingredient being prepared is tested.
Purify the test of air active ingredient validity duration:
It is new to prepare purification air active ingredient
In 5m3Confined chamber in, input formaldehyde, benzene or ammonia gas thereto, open closed indoor fan, 30min post-sampling is close
The initial concentration of gas in closed chamber.Then in 5m3In the confined chamber of sealing, just purification air active ingredient obtained is sprayed to
Wherein, sample gas concentration immediately after 24 hours calculates removal rate.Removal rate=(concentration after initial concentration -24 hours) ×
100/ initial concentration, control group is used sprays into water as blank control in confined chamber；Testing result is shown in Table 1.
Table 1
Place 1 month purification air active ingredient
Purification air active ingredient is configured, is sprayed into confined chamber, with same test after one month, is inputted into confined chamber
Same amount of formaldehyde, benzene or ammonia gas open closed indoor fan, acquire gas concentration after 24 hours；Wherein, TVOC is always to wave
Hair property organic matter, all interior organic gas substances.Testing result is shown in Table 2
Table 2
Above embodiment is merely exemplary to illustrate the principle of the present invention and its effect, and is not intended to limit the present invention.For being familiar with
The people of this technology all without departing from the spirit and scope of the present invention, modifies above-described embodiment or is improved.Therefore, all
It lifts in the technical field and has what usually intellectual was completed without departing from the spirit and technical ideas disclosed in the present invention
All equivalent modifications or change should be covered by the claims of the present invention.
Claims (8)
1. a kind of air combined biologically active agents of purification, it is characterised in that: each component is according to following weight: microorganism
It 5-10 parts of bacterium solution, 8-20 parts of organized enzyme, 0.5-2 parts of plant polyose, 20-45 parts of traditional Chinese medicine extraction mixed liquor, 1-8 parts of chitin, goes
45-75 parts of ionized water；
The microbial inoculum is lactic acid bacteria, photosynthetic bacteria, saccharomycete, bacillus subtilis, pseudomonad and rhizopus；
The organized enzyme is lysozyme, glucose oxidase, lignin peroxidase, protease, lipase and Lumbrokinase；
The plant polyose is polysaccharides from ginkgo biloba, pheophytin and sea grass polysaccharide；
The traditional Chinese medicine extraction mixed liquor is the extract mixed liquor of Radix Isatidis, honeysuckle, purslane, cordate houttuynia and radix scutellariae.
2. a kind of air combined biologically active agents of purification according to claim 1, it is characterised in that: the microbial bacteria
The mixed volume ratio of each bacterium solution of liquid is lactic acid bacteria: photosynthetic bacteria: saccharomycete: bacillus subtilis: pseudomonad: rhizopus=1-
5:1-2:1-2:1-3:1-2:0.5-1。
3. a kind of air combined biologically active agents of purification according to claim 1, it is characterised in that: the organized enzyme is each
The weight of component are as follows: 10-15 parts of lysozyme, 5-10 parts of glucose oxidase, 1-5 parts of lignin peroxidase, egg
White enzyme 10-25 parts, 12-20 parts of lipase, 1-5 parts of Lumbrokinase.
4. a kind of air combined biologically active agents of purification according to claim 1, it is characterised in that: the plant polyose
Each group distribution ratio is polysaccharides from ginkgo biloba 3-8 parts, 0.1-0.5 parts of pheophytin, 5-10 parts of sea grass polysaccharide.
5. a kind of air combined biologically active agents of purification according to claim 1, it is characterised in that: the traditional Chinese medicine extraction
Mixed liquor is that Radix Isatidis, honeysuckle, purslane, cordate houttuynia and radix scutellariae extracting solution are mixed to prepare according to parts by weight 1:1:1:1:1.
6. a kind of air combined biologically active agents of purification according to claim 1, it is characterised in that: the chitin point
Son amount is 7000-10000.
7. a kind of air combined biologically active agents preparation method of purification described in claim 1, it is characterised in that according to following
Step carries out:
1) microbial inoculum is produced
After the activation of lactic acid bacteria, photosynthetic bacteria, saccharomycete, bacillus subtilis, pseudomonad and rhizopus, it is inoculated in ox respectively
In meat extract peptone culture medium, in 37 DEG C, shaking table culture for 24 hours, lactobacillus solution, photosynthetic bacteria seed liquor, saccharomycete is made
Seed liquor, bacillus subtilis seed liquor, pseudomonad seed liquor and rhizopus seed liquor；
By lactobacillus solution, photosynthetic bacteria seed liquor, saccharomycete seed liquor, bacillus subtilis seed liquor, pseudomonad kind
Sub- liquid and the mixing of rhizopus seed liquor, are inoculated in mixing fermentation culture for mixed bacteria liquid with the inoculum concentration of percent by volume 8-15%
In base, shaking table culture 2-3d under the conditions of 37 DEG C, 180r/min；
Then bacterium solution 3000-5000r/min culture obtained is centrifuged 5-8min, takes supernatant to get microbial inoculum is arrived；
2) traditional Chinese medicine extraction mixed liquor
The Radix Isatidis, honeysuckle, purslane, cordate houttuynia and radix scutellariae are weighed by weight, and each medicinal material extracts extracting solution respectively, will
Pulverizing medicinal materials are mixed with its 3-6 times water, are then successively digested through cellulase, pectase, protease；Hydrolysis temperature is
25-35 DEG C, the time is respectively 1-3h；It is then centrifuged for isolated supernatant, Chinese medicine is obtained after the supernatant of each medicinal material is mixed and mentions
Take mixed liquor；
3) plant polyose and organized enzyme
The lysozyme, glucose oxidase, lignin peroxidase, protease, lipase, Lumbrokinase are weighed, ginkgo is more
Sugar, pheophytin and sea grass polysaccharide pass through ultrafiltration membrance filter with the deionized water mixed dissolution under the conditions of 0.1-0.3MPa,
Impurity is removed, 1-3h is stood, obtains plant polyose and active enzyme solutions；
4) solution obtained by step 1) and 2) is mixed according to the solution that parts by weight are obtained with step 3), adds the first
Shell element, is sufficiently mixed and uniformly purifies air active ingredient to get to finished product.
8. a kind of air combined biologically active agents preparation method of purification according to claim 7, it is characterised in that: described
Mixed fermentive culture medium are as follows: glucose 15g/l, soy peptone 4.8g/l, yeast extract 2.5g/l, ammonium citrate 1.8g/l,
Sodium acetate 5g/l, K2HPO42g/l, Tween 80 1ml/l, MgSO4·7H2O 0.6g/l, MnSO4·4H2O:0.3g/l, water are fixed
Hold 1L.
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