JP2011527570A5 - - Google Patents
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- Publication number
- JP2011527570A5 JP2011527570A5 JP2011517379A JP2011517379A JP2011527570A5 JP 2011527570 A5 JP2011527570 A5 JP 2011527570A5 JP 2011517379 A JP2011517379 A JP 2011517379A JP 2011517379 A JP2011517379 A JP 2011517379A JP 2011527570 A5 JP2011527570 A5 JP 2011527570A5
- Authority
- JP
- Japan
- Prior art keywords
- thio
- nucleic acid
- dna polymerase
- dttp
- group
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108020004414 DNA Proteins 0.000 claims 17
- 102000053602 DNA Human genes 0.000 claims 16
- 238000000034 method Methods 0.000 claims 14
- 102000039446 nucleic acids Human genes 0.000 claims 14
- 108020004707 nucleic acids Proteins 0.000 claims 14
- 150000007523 nucleic acids Chemical class 0.000 claims 14
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims 11
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims 11
- 125000003729 nucleotide group Chemical group 0.000 claims 10
- 108020004682 Single-Stranded DNA Proteins 0.000 claims 8
- 230000028993 immune response Effects 0.000 claims 6
- 244000045947 parasite Species 0.000 claims 6
- NGYHUCPPLJOZIX-XLPZGREQSA-N 5-methyl-dCTP Chemical compound O=C1N=C(N)C(C)=CN1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C1 NGYHUCPPLJOZIX-XLPZGREQSA-N 0.000 claims 5
- AHCYMLUZIRLXAA-SHYZEUOFSA-N Deoxyuridine 5'-triphosphate Chemical compound O1[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C[C@@H]1N1C(=O)NC(=O)C=C1 AHCYMLUZIRLXAA-SHYZEUOFSA-N 0.000 claims 5
- 108091028733 RNTP Proteins 0.000 claims 5
- UFJPAQSLHAGEBL-RRKCRQDMSA-N dITP Chemical compound O1[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C[C@@H]1N1C(N=CNC2=O)=C2N=C1 UFJPAQSLHAGEBL-RRKCRQDMSA-N 0.000 claims 5
- 239000002773 nucleotide Substances 0.000 claims 5
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims 4
- 230000000694 effects Effects 0.000 claims 4
- 229960005486 vaccine Drugs 0.000 claims 4
- 108010017826 DNA Polymerase I Proteins 0.000 claims 3
- 102000004594 DNA Polymerase I Human genes 0.000 claims 3
- 108060002716 Exonuclease Proteins 0.000 claims 3
- 102000013165 exonuclease Human genes 0.000 claims 3
- 239000013615 primer Substances 0.000 claims 3
- 238000001890 transfection Methods 0.000 claims 3
- 241000894006 Bacteria Species 0.000 claims 2
- 241000233866 Fungi Species 0.000 claims 2
- 108091034117 Oligonucleotide Proteins 0.000 claims 2
- 108020004459 Small interfering RNA Proteins 0.000 claims 2
- 241000700605 Viruses Species 0.000 claims 2
- 239000000427 antigen Substances 0.000 claims 2
- 102000036639 antigens Human genes 0.000 claims 2
- 108091007433 antigens Proteins 0.000 claims 2
- 230000001939 inductive effect Effects 0.000 claims 2
- 108020004999 messenger RNA Proteins 0.000 claims 2
- 108091070501 miRNA Proteins 0.000 claims 2
- 239000002679 microRNA Substances 0.000 claims 2
- 239000004055 small Interfering RNA Substances 0.000 claims 2
- 241000322342 Bacillus phage M2 Species 0.000 claims 1
- 108020004638 Circular DNA Proteins 0.000 claims 1
- 239000003155 DNA primer Substances 0.000 claims 1
- 108700007698 Genetic Terminator Regions Proteins 0.000 claims 1
- 108010025076 Holoenzymes Proteins 0.000 claims 1
- 101150054516 PRD1 gene Proteins 0.000 claims 1
- 101100459905 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) NCP1 gene Proteins 0.000 claims 1
- 108010006785 Taq Polymerase Proteins 0.000 claims 1
- 108010020713 Tth polymerase Proteins 0.000 claims 1
- 238000000137 annealing Methods 0.000 claims 1
- 230000001580 bacterial effect Effects 0.000 claims 1
- 230000000295 complement effect Effects 0.000 claims 1
- 239000005547 deoxyribonucleotide Substances 0.000 claims 1
- 125000002637 deoxyribonucleotide group Chemical group 0.000 claims 1
- 238000006073 displacement reaction Methods 0.000 claims 1
- 230000002538 fungal effect Effects 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- 108091027963 non-coding RNA Proteins 0.000 claims 1
- 102000042567 non-coding RNA Human genes 0.000 claims 1
- 102000004169 proteins and genes Human genes 0.000 claims 1
- 108090000623 proteins and genes Proteins 0.000 claims 1
- 238000003151 transfection method Methods 0.000 claims 1
- 235000011178 triphosphate Nutrition 0.000 claims 1
- 239000001226 triphosphate Substances 0.000 claims 1
- UNXRWKVEANCORM-UHFFFAOYSA-N triphosphoric acid Chemical compound OP(O)(=O)OP(O)(=O)OP(O)(O)=O UNXRWKVEANCORM-UHFFFAOYSA-N 0.000 claims 1
- 241001515965 unidentified phage Species 0.000 claims 1
- 230000003612 virological effect Effects 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US12/169,993 US20100008939A1 (en) | 2008-07-09 | 2008-07-09 | Unprocessed rolling circle amplification product |
| US12/169,993 | 2008-07-09 | ||
| PCT/SE2009/050667 WO2010005365A1 (en) | 2008-07-09 | 2009-06-04 | Unprocessed rolling circle amplification product |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2011527570A JP2011527570A (ja) | 2011-11-04 |
| JP2011527570A5 true JP2011527570A5 (https=) | 2012-05-31 |
| JP5600675B2 JP5600675B2 (ja) | 2014-10-01 |
Family
ID=41505352
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2011517379A Active JP5600675B2 (ja) | 2008-07-09 | 2009-06-04 | 未処理ｒｃａ産物 |
Country Status (4)
| Country | Link |
|---|---|
| US (1) | US20100008939A1 (https=) |
| EP (1) | EP2307575B1 (https=) |
| JP (1) | JP5600675B2 (https=) |
| WO (1) | WO2010005365A1 (https=) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110195457A1 (en) * | 2010-02-09 | 2011-08-11 | General Electric Company | Isothermal amplification of nucleic acid using primers comprising a randomized sequence and specific primers and uses thereof |
| US9644232B2 (en) * | 2013-07-26 | 2017-05-09 | General Electric Company | Method and device for collection and amplification of circulating nucleic acids |
| JP6080884B2 (ja) | 2014-03-28 | 2017-02-15 | 富士フイルム株式会社 | 重合性化合物、ポリマー、重合性組成物、フィルム、および投映像表示用ハーフミラー |
| WO2016170179A1 (en) * | 2015-04-24 | 2016-10-27 | Qiagen Gmbh | Method for immobilizing a nucleic acid molecule on solid support |
| US11085073B2 (en) | 2015-04-24 | 2021-08-10 | Qiagen Gmbh | Method for immobilizing a nucleic acid molecule on a solid support |
| EP3297715B1 (en) * | 2015-05-18 | 2020-09-16 | Scott Schaffer | Clip-on nasal air humidifying and epistaxis-prevention device |
| US10077459B2 (en) | 2016-05-04 | 2018-09-18 | General Electric Company | Cell-free protein expression using rolling circle amplification product |
| US10350307B2 (en) | 2017-09-18 | 2019-07-16 | General Electric Company | In vivo RNA or protein expression using double-stranded concatemeric DNA including phosphorothioated nucleotides |
| US12595501B2 (en) | 2019-06-13 | 2026-04-07 | Global Life Sciences Solutions Usa Llc | Expression of products from nucleic acid concatemers |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5714320A (en) * | 1993-04-15 | 1998-02-03 | University Of Rochester | Rolling circle synthesis of oligonucleotides and amplification of select randomized circular oligonucleotides |
| US7030098B2 (en) * | 1999-03-12 | 2006-04-18 | The Board Of Trustees Of The Leland Stanford Junior University | DNA vaccination for treatment of autoimmune disease |
| AU2001238535A1 (en) * | 2000-02-16 | 2001-08-27 | Large Scale Biology Corporation | Rolling circle replicon expression vectors |
| US6323009B1 (en) * | 2000-06-28 | 2001-11-27 | Molecular Staging, Inc. | Multiply-primed amplification of nucleic acid sequences |
| CU23400A1 (es) * | 2003-04-29 | 2009-08-04 | Ct Ingenieria Genetica Biotech | Método para la obtención de minicírculos replicativos de adn para transferencia génica o como inmunomoduladores |
| US20050202453A1 (en) * | 2003-05-28 | 2005-09-15 | Wen-Yuan Song | Nucleic acid amplification in yeast |
| CN101103122A (zh) * | 2004-12-11 | 2008-01-09 | 西托吉尼克斯公司 | 高质量核酸的无细胞生物合成及其用途 |
| US20080305142A1 (en) * | 2004-12-11 | 2008-12-11 | Cytogenix, Inc. | Cell Free Biosynthesis of High-Quality Nucleic Acid and Uses Thereof |
| WO2007018744A2 (en) * | 2005-08-03 | 2007-02-15 | Cytogenix, Inc. | Cell-free biosynthesis of nucleic acid |
-
2008
- 2008-07-09 US US12/169,993 patent/US20100008939A1/en not_active Abandoned
-
2009
- 2009-06-04 WO PCT/SE2009/050667 patent/WO2010005365A1/en not_active Ceased
- 2009-06-04 EP EP09794720.4A patent/EP2307575B1/en active Active
- 2009-06-04 JP JP2011517379A patent/JP5600675B2/ja active Active
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