JP2011504732A5 - - Google Patents

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Publication number
JP2011504732A5
JP2011504732A5 JP2010535283A JP2010535283A JP2011504732A5 JP 2011504732 A5 JP2011504732 A5 JP 2011504732A5 JP 2010535283 A JP2010535283 A JP 2010535283A JP 2010535283 A JP2010535283 A JP 2010535283A JP 2011504732 A5 JP2011504732 A5 JP 2011504732A5
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JP
Japan
Prior art keywords
dna polymerase
primer
group
nucleotide
polymerase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Application number
JP2010535283A
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English (en)
Japanese (ja)
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JP5596554B2 (ja
JP2011504732A (ja
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Priority claimed from PCT/EP2008/010036 external-priority patent/WO2009068268A1/en
Publication of JP2011504732A publication Critical patent/JP2011504732A/ja
Publication of JP2011504732A5 publication Critical patent/JP2011504732A5/ja
Application granted granted Critical
Publication of JP5596554B2 publication Critical patent/JP5596554B2/ja
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

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JP2010535283A 2007-11-28 2008-11-26 向上したピロリン酸分解活性化重合（ｐａｐ）能力を有する変異ｄｎａポリメラーゼ Expired - Fee Related JP5596554B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US99084707P 2007-11-28 2007-11-28
US60/990,847 2007-11-28
PCT/EP2008/010036 WO2009068268A1 (en) 2007-11-28 2008-11-26 Mutant dna polymerases with improved pyrophosphorolysis activated polymerization (pap) ability

Publications (3)

Publication Number Publication Date
JP2011504732A JP2011504732A (ja) 2011-02-17
JP2011504732A5 true JP2011504732A5 (cg-RX-API-DMAC7.html) 2011-08-18
JP5596554B2 JP5596554B2 (ja) 2014-09-24

Family

ID=40340463

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2010535283A Expired - Fee Related JP5596554B2 (ja) 2007-11-28 2008-11-26 向上したピロリン酸分解活性化重合（ｐａｐ）能力を有する変異ｄｎａポリメラーゼ

Country Status (6)

Country Link
US (1) US8071536B2 (cg-RX-API-DMAC7.html)
EP (1) EP2215222B1 (cg-RX-API-DMAC7.html)
JP (1) JP5596554B2 (cg-RX-API-DMAC7.html)
CA (1) CA2706999C (cg-RX-API-DMAC7.html)
ES (1) ES2553893T3 (cg-RX-API-DMAC7.html)
WO (1) WO2009068268A1 (cg-RX-API-DMAC7.html)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5876477B2 (ja) 2010-06-18 2016-03-02 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 増大した３’末端ミスマッチ識別能を有するｄｎａポリメラーゼ
EP2652152A4 (en) * 2010-12-13 2014-06-25 Life Technologies Corp POLYMERIZATION OF NUCLEIC ACIDS BY ACTIVATION BY POLYPHOSPHOROLYSIS REACTIONS (APP)
PT3004378T (pt) 2013-05-24 2018-03-06 Illumina Cambridge Ltd Resumo
US11921049B2 (en) * 2018-11-09 2024-03-05 Shaofeng Ding Pyrophosphorolysis activated fluorescence to measure PAP amplification of nucleic acid

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6228628B1 (en) * 1997-07-09 2001-05-08 Roche Molecular Systems Mutant chimeric DNA polymerase
US6329178B1 (en) * 2000-01-14 2001-12-11 University Of Washington DNA polymerase mutant having one or more mutations in the active site
ES2330408T3 (es) * 2000-02-23 2009-12-10 City Of Hope Polimerizacion activada por pirofosforolisis (pap): aplicacion en la amplificacion de un alelo especifico y en la determinacion de secuencias de acidos nucleicos.
US7148049B2 (en) * 2002-04-02 2006-12-12 Roche Molecular Systems, Inc. Thermostable or thermoactive DNA polymerase molecules with attenuated 3′-5′ exonuclease activity
JP4500673B2 (ja) * 2002-05-10 2010-07-14 シティ・オブ・ホープ 加ピロリン酸分解活性化重合（ｐａｐ）
SE0202831D0 (sv) * 2002-09-25 2002-09-25 Siemens Elema Ab Apparat för bestämning av rekryterbar volym i en lunga
US7572581B2 (en) * 2003-06-30 2009-08-11 Roche Molecular Systems, Inc. 2′-terminator nucleotide-related methods and systems
US7745125B2 (en) * 2004-06-28 2010-06-29 Roche Molecular Systems, Inc. 2′-terminator related pyrophosphorolysis activated polymerization
US8962293B2 (en) * 2006-10-18 2015-02-24 Roche Molecular Systems, Inc. DNA polymerases and related methods
US8026091B2 (en) 2007-07-13 2011-09-27 Roche Molecular Systems, Inc. DNA polymerases and related methods

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