CA2706999C - Mutant dna polymerases with improved pyrophosphorolysis activated polymerization (pap) ability - Google Patents
Mutant dna polymerases with improved pyrophosphorolysis activated polymerization (pap) ability Download PDFInfo
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- CA2706999C CA2706999C CA2706999A CA2706999A CA2706999C CA 2706999 C CA2706999 C CA 2706999C CA 2706999 A CA2706999 A CA 2706999A CA 2706999 A CA2706999 A CA 2706999A CA 2706999 C CA2706999 C CA 2706999C
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- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 1
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- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 description 1
- YOBAEOGBNPPUQV-UHFFFAOYSA-N iron;trihydrate Chemical compound O.O.O.[Fe].[Fe] YOBAEOGBNPPUQV-UHFFFAOYSA-N 0.000 description 1
- 230000002427 irreversible effect Effects 0.000 description 1
- AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 description 1
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- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 229930182817 methionine Natural products 0.000 description 1
- 239000000178 monomer Substances 0.000 description 1
- 230000000869 mutational effect Effects 0.000 description 1
- 210000004897 n-terminal region Anatomy 0.000 description 1
- 239000002853 nucleic acid probe Substances 0.000 description 1
- 125000003835 nucleoside group Chemical group 0.000 description 1
- 229940124276 oligodeoxyribonucleotide Drugs 0.000 description 1
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- 150000007524 organic acids Chemical class 0.000 description 1
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- PSWJVKKJYCAPTI-UHFFFAOYSA-N oxido-oxo-phosphonophosphanylphosphanium Chemical compound OP(O)(=O)PP(=O)=O PSWJVKKJYCAPTI-UHFFFAOYSA-N 0.000 description 1
- LDCYZAJDBXYCGN-UHFFFAOYSA-N oxitriptan Natural products C1=C(O)C=C2C(CC(N)C(O)=O)=CNC2=C1 LDCYZAJDBXYCGN-UHFFFAOYSA-N 0.000 description 1
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- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 1
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- 150000004713 phosphodiesters Chemical class 0.000 description 1
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- 229920002477 rna polymer Polymers 0.000 description 1
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- KYMBYSLLVAOCFI-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SCN1CC1=CN=C(C)N=C1N KYMBYSLLVAOCFI-UHFFFAOYSA-N 0.000 description 1
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- 150000003573 thiols Chemical class 0.000 description 1
- RYYWUUFWQRZTIU-UHFFFAOYSA-K thiophosphate Chemical compound [O-]P([O-])([O-])=S RYYWUUFWQRZTIU-UHFFFAOYSA-K 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US99084707P | 2007-11-28 | 2007-11-28 | |
| US60/990,847 | 2007-11-28 | ||
| PCT/EP2008/010036 WO2009068268A1 (en) | 2007-11-28 | 2008-11-26 | Mutant dna polymerases with improved pyrophosphorolysis activated polymerization (pap) ability |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2706999A1 CA2706999A1 (en) | 2009-06-04 |
| CA2706999C true CA2706999C (en) | 2013-01-22 |
Family
ID=40340463
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2706999A Expired - Fee Related CA2706999C (en) | 2007-11-28 | 2008-11-26 | Mutant dna polymerases with improved pyrophosphorolysis activated polymerization (pap) ability |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US8071536B2 (cg-RX-API-DMAC7.html) |
| EP (1) | EP2215222B1 (cg-RX-API-DMAC7.html) |
| JP (1) | JP5596554B2 (cg-RX-API-DMAC7.html) |
| CA (1) | CA2706999C (cg-RX-API-DMAC7.html) |
| ES (1) | ES2553893T3 (cg-RX-API-DMAC7.html) |
| WO (1) | WO2009068268A1 (cg-RX-API-DMAC7.html) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP5876477B2 (ja) | 2010-06-18 | 2016-03-02 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | 増大した３’末端ミスマッチ識別能を有するｄｎａポリメラーゼ |
| EP2652152A4 (en) * | 2010-12-13 | 2014-06-25 | Life Technologies Corp | POLYMERIZATION OF NUCLEIC ACIDS BY ACTIVATION BY POLYPHOSPHOROLYSIS REACTIONS (APP) |
| PT3004378T (pt) | 2013-05-24 | 2018-03-06 | Illumina Cambridge Ltd | Resumo |
| US11921049B2 (en) * | 2018-11-09 | 2024-03-05 | Shaofeng Ding | Pyrophosphorolysis activated fluorescence to measure PAP amplification of nucleic acid |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6228628B1 (en) * | 1997-07-09 | 2001-05-08 | Roche Molecular Systems | Mutant chimeric DNA polymerase |
| US6329178B1 (en) * | 2000-01-14 | 2001-12-11 | University Of Washington | DNA polymerase mutant having one or more mutations in the active site |
| ES2330408T3 (es) * | 2000-02-23 | 2009-12-10 | City Of Hope | Polimerizacion activada por pirofosforolisis (pap): aplicacion en la amplificacion de un alelo especifico y en la determinacion de secuencias de acidos nucleicos. |
| US7148049B2 (en) * | 2002-04-02 | 2006-12-12 | Roche Molecular Systems, Inc. | Thermostable or thermoactive DNA polymerase molecules with attenuated 3′-5′ exonuclease activity |
| JP4500673B2 (ja) * | 2002-05-10 | 2010-07-14 | シティ・オブ・ホープ | 加ピロリン酸分解活性化重合（ｐａｐ） |
| SE0202831D0 (sv) * | 2002-09-25 | 2002-09-25 | Siemens Elema Ab | Apparat för bestämning av rekryterbar volym i en lunga |
| US7572581B2 (en) * | 2003-06-30 | 2009-08-11 | Roche Molecular Systems, Inc. | 2′-terminator nucleotide-related methods and systems |
| US7745125B2 (en) * | 2004-06-28 | 2010-06-29 | Roche Molecular Systems, Inc. | 2′-terminator related pyrophosphorolysis activated polymerization |
| US8962293B2 (en) * | 2006-10-18 | 2015-02-24 | Roche Molecular Systems, Inc. | DNA polymerases and related methods |
| US8026091B2 (en) | 2007-07-13 | 2011-09-27 | Roche Molecular Systems, Inc. | DNA polymerases and related methods |
-
2008
- 2008-11-26 EP EP08855304.5A patent/EP2215222B1/en not_active Not-in-force
- 2008-11-26 ES ES08855304.5T patent/ES2553893T3/es active Active
- 2008-11-26 WO PCT/EP2008/010036 patent/WO2009068268A1/en not_active Ceased
- 2008-11-26 US US12/324,491 patent/US8071536B2/en not_active Expired - Fee Related
- 2008-11-26 CA CA2706999A patent/CA2706999C/en not_active Expired - Fee Related
- 2008-11-26 JP JP2010535283A patent/JP5596554B2/ja not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| WO2009068268A1 (en) | 2009-06-04 |
| ES2553893T3 (es) | 2015-12-14 |
| JP5596554B2 (ja) | 2014-09-24 |
| CA2706999A1 (en) | 2009-06-04 |
| US20090155802A1 (en) | 2009-06-18 |
| EP2215222B1 (en) | 2015-09-16 |
| EP2215222A1 (en) | 2010-08-11 |
| US8071536B2 (en) | 2011-12-06 |
| JP2011504732A (ja) | 2011-02-17 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| MKLA | Lapsed |
Effective date: 20211126 |