JP2011500757A5 - - Google Patents

Info

Publication number

JP2011500757A5

JP2011500757A5 JP2010530429A JP2010530429A JP2011500757A5 JP 2011500757 A5 JP2011500757 A5 JP 2011500757A5 JP 2010530429 A JP2010530429 A JP 2010530429A JP 2010530429 A JP2010530429 A JP 2010530429A JP 2011500757 A5 JP2011500757 A5 JP 2011500757A5

Authority

JP

Japan

Prior art keywords

resin

protein

containing protein

affinity chromatography

polypeptide

Prior art date

2008-10-21

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Withdrawn

Links

• Espacenet
• Global Dossier
• Discuss

• 238000000034 method Methods 0.000 claims 27
• 102000004169 proteins and genes Human genes 0.000 claims 13
• 108090000623 proteins and genes Proteins 0.000 claims 13
• 239000011347 resin Substances 0.000 claims 8
• 229920005989 resin Polymers 0.000 claims 8
• 238000001042 affinity chromatography Methods 0.000 claims 7
• 229920001184 polypeptide Polymers 0.000 claims 7
• 102000004196 processed proteins & peptides Human genes 0.000 claims 7
• 108090000765 processed proteins & peptides Proteins 0.000 claims 7
• WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims 6
• 108091006020 Fc-tagged proteins Proteins 0.000 claims 5
• 239000001045 blue dye Substances 0.000 claims 5
• 239000012539 chromatography resin Substances 0.000 claims 4
• 229920002684 Sepharose Polymers 0.000 claims 3
• 239000003446 ligand Substances 0.000 claims 3
• 239000007788 liquid Substances 0.000 claims 3
• 235000011164 potassium chloride Nutrition 0.000 claims 3
• 239000001103 potassium chloride Substances 0.000 claims 3
• 150000003839 salts Chemical class 0.000 claims 3
• 108060003951 Immunoglobulin Proteins 0.000 claims 2
• 102000008300 Mutant Proteins Human genes 0.000 claims 2
• 108010021466 Mutant Proteins Proteins 0.000 claims 2
• FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims 2
• 108060008683 Tumor Necrosis Factor Receptor Proteins 0.000 claims 2
• 238000004587 chromatography analysis Methods 0.000 claims 2
• 230000000295 complement effect Effects 0.000 claims 2
• 239000012634 fragment Substances 0.000 claims 2
• 102000018358 immunoglobulin Human genes 0.000 claims 2
• 239000000203 mixture Substances 0.000 claims 2
• 102000040430 polynucleotide Human genes 0.000 claims 2
• 108091033319 polynucleotide Proteins 0.000 claims 2
• 239000002157 polynucleotide Substances 0.000 claims 2
• 102000003298 tumor necrosis factor receptor Human genes 0.000 claims 2
• BGFTWECWAICPDG-UHFFFAOYSA-N 2-[bis(4-chlorophenyl)methyl]-4-n-[3-[bis(4-chlorophenyl)methyl]-4-(dimethylamino)phenyl]-1-n,1-n-dimethylbenzene-1,4-diamine Chemical compound C1=C(C(C=2C=CC(Cl)=CC=2)C=2C=CC(Cl)=CC=2)C(N(C)C)=CC=C1NC(C=1)=CC=C(N(C)C)C=1C(C=1C=CC(Cl)=CC=1)C1=CC=C(Cl)C=C1 BGFTWECWAICPDG-UHFFFAOYSA-N 0.000 claims 1
• FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims 1
• 208000002109 Argyria Diseases 0.000 claims 1
• 108010008014 B-Cell Maturation Antigen Proteins 0.000 claims 1
• 102000006942 B-Cell Maturation Antigen Human genes 0.000 claims 1
• 101000795167 Homo sapiens Tumor necrosis factor receptor superfamily member 13B Proteins 0.000 claims 1
• 102000017727 Immunoglobulin Variable Region Human genes 0.000 claims 1
• 108010067060 Immunoglobulin Variable Region Proteins 0.000 claims 1
• 102100026720 Interferon beta Human genes 0.000 claims 1
• 108090000467 Interferon-beta Proteins 0.000 claims 1
• VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims 1
• 102100040247 Tumor necrosis factor Human genes 0.000 claims 1
• 102100029675 Tumor necrosis factor receptor superfamily member 13B Human genes 0.000 claims 1
• 102100029690 Tumor necrosis factor receptor superfamily member 13C Human genes 0.000 claims 1
• 101710178300 Tumor necrosis factor receptor superfamily member 13C Proteins 0.000 claims 1
• 102100033732 Tumor necrosis factor receptor superfamily member 1A Human genes 0.000 claims 1
• 101710187743 Tumor necrosis factor receptor superfamily member 1A Proteins 0.000 claims 1
• 101710187830 Tumor necrosis factor receptor superfamily member 1B Proteins 0.000 claims 1
• 102100033733 Tumor necrosis factor receptor superfamily member 1B Human genes 0.000 claims 1
• 150000001413 amino acids Chemical class 0.000 claims 1
• YKCWQPZFAFZLBI-UHFFFAOYSA-N cibacron blue Chemical compound C1=2C(=O)C3=CC=CC=C3C(=O)C=2C(N)=C(S(O)(=O)=O)C=C1NC(C=C1S(O)(=O)=O)=CC=C1NC(N=1)=NC(Cl)=NC=1NC1=CC=CC=C1S(O)(=O)=O YKCWQPZFAFZLBI-UHFFFAOYSA-N 0.000 claims 1
• 239000000975 dye Substances 0.000 claims 1
• 239000012530 fluid Substances 0.000 claims 1
• 238000004191 hydrophobic interaction chromatography Methods 0.000 claims 1
• 229910052588 hydroxylapatite Inorganic materials 0.000 claims 1
• 238000004255 ion exchange chromatography Methods 0.000 claims 1
• XYJRXVWERLGGKC-UHFFFAOYSA-D pentacalcium;hydroxide;triphosphate Chemical compound [OH-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O XYJRXVWERLGGKC-UHFFFAOYSA-D 0.000 claims 1
• 239000008194 pharmaceutical composition Substances 0.000 claims 1
• 235000017281 sodium acetate Nutrition 0.000 claims 1
• 239000001632 sodium acetate Substances 0.000 claims 1
• 239000011780 sodium chloride Substances 0.000 claims 1
• 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 claims 1
• 238000000108 ultra-filtration Methods 0.000 claims 1
• 238000005406 washing Methods 0.000 claims 1