JP2010534477A5 - - Google Patents
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- Publication number
- JP2010534477A5 JP2010534477A5 JP2010518341A JP2010518341A JP2010534477A5 JP 2010534477 A5 JP2010534477 A5 JP 2010534477A5 JP 2010518341 A JP2010518341 A JP 2010518341A JP 2010518341 A JP2010518341 A JP 2010518341A JP 2010534477 A5 JP2010534477 A5 JP 2010534477A5
- Authority
- JP
- Japan
- Prior art keywords
- peptide
- gln
- inclusion body
- interest
- body tag
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 67
- 230000004927 fusion Effects 0.000 claims description 24
- 210000003000 inclusion body Anatomy 0.000 claims description 22
- 108090000623 proteins and genes Proteins 0.000 claims description 12
- 210000004027 cell Anatomy 0.000 claims description 11
- 238000000034 method Methods 0.000 claims description 7
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 claims description 4
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims description 4
- 125000000151 cysteine group Chemical group N[C@@H](CS)C(=O)* 0.000 claims description 4
- 125000006850 spacer group Chemical group 0.000 claims description 4
- 230000001131 transforming effect Effects 0.000 claims description 4
- 108020004707 nucleic acids Proteins 0.000 claims description 3
- 102000039446 nucleic acids Human genes 0.000 claims description 3
- 150000007523 nucleic acids Chemical class 0.000 claims description 3
- 230000002194 synthesizing effect Effects 0.000 claims description 3
- 239000004475 Arginine Substances 0.000 claims description 2
- 239000004471 Glycine Substances 0.000 claims description 2
- XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 claims description 2
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 claims description 2
- ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 claims description 2
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 claims description 2
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 claims description 2
- 235000001014 amino acid Nutrition 0.000 claims description 2
- 150000001413 amino acids Chemical class 0.000 claims description 2
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 claims description 2
- 210000004899 c-terminal region Anatomy 0.000 claims description 2
- 238000003776 cleavage reaction Methods 0.000 claims description 2
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 claims description 2
- 235000018417 cysteine Nutrition 0.000 claims description 2
- 235000013922 glutamic acid Nutrition 0.000 claims description 2
- 239000004220 glutamic acid Substances 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 claims description 2
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 claims description 2
- 230000007017 scission Effects 0.000 claims description 2
- 241000235648 Pichia Species 0.000 description 4
- 241000589565 Flavobacterium Species 0.000 description 2
- 241000589345 Methylococcus Species 0.000 description 2
- 241000186359 Mycobacterium Species 0.000 description 2
- 229920002678 cellulose Polymers 0.000 description 2
- 239000001913 cellulose Substances 0.000 description 2
- 239000004927 clay Substances 0.000 description 2
- 238000010367 cloning Methods 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 239000000049 pigment Substances 0.000 description 2
- 229920000642 polymer Polymers 0.000 description 2
- 239000003910 polypeptide antibiotic agent Substances 0.000 description 2
- 241000589291 Acinetobacter Species 0.000 description 1
- 241000589158 Agrobacterium Species 0.000 description 1
- 102000044503 Antimicrobial Peptides Human genes 0.000 description 1
- 108700042778 Antimicrobial Peptides Proteins 0.000 description 1
- 241000203069 Archaea Species 0.000 description 1
- 241000228212 Aspergillus Species 0.000 description 1
- 241000186146 Brevibacterium Species 0.000 description 1
- 241000222120 Candida <Saccharomycetales> Species 0.000 description 1
- 241000191366 Chlorobium Species 0.000 description 1
- 241000190831 Chromatium Species 0.000 description 1
- 241000186216 Corynebacterium Species 0.000 description 1
- 241000588698 Erwinia Species 0.000 description 1
- 241000190844 Erythrobacter Species 0.000 description 1
- 241000588748 Klebsiella Species 0.000 description 1
- 241000589350 Methylobacter Species 0.000 description 1
- 241000589966 Methylocystis Species 0.000 description 1
- 241001533203 Methylomicrobium Species 0.000 description 1
- 241000589344 Methylomonas Species 0.000 description 1
- 241000863420 Myxococcus Species 0.000 description 1
- 241000520272 Pantoea Species 0.000 description 1
- 241000589516 Pseudomonas Species 0.000 description 1
- 241000191025 Rhodobacter Species 0.000 description 1
- 241000316848 Rhodococcus <scale insect> Species 0.000 description 1
- 241000235070 Saccharomyces Species 0.000 description 1
- 241000736131 Sphingomonas Species 0.000 description 1
- 241000187747 Streptomyces Species 0.000 description 1
- 241000223259 Trichoderma Species 0.000 description 1
- 241000235013 Yarrowia Species 0.000 description 1
- 241000588901 Zymomonas Species 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US11/782,836 | 2007-07-25 | ||
| US11/782,836 US7678883B2 (en) | 2007-07-25 | 2007-07-25 | Solubility tags for the expression and purification of bioactive peptides |
| PCT/US2008/070802 WO2009015165A1 (en) | 2007-07-25 | 2008-07-23 | Solubility tags for the expression and purification of bioactive peptides |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2010534477A JP2010534477A (ja) | 2010-11-11 |
| JP2010534477A5 true JP2010534477A5 (https=) | 2011-08-18 |
| JP5453261B2 JP5453261B2 (ja) | 2014-03-26 |
Family
ID=39967949
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2010518341A Expired - Fee Related JP5453261B2 (ja) | 2007-07-25 | 2008-07-23 | 生物活性ペプチドの発現および精製のための可溶性タグ |
Country Status (10)
| Country | Link |
|---|---|
| US (2) | US7678883B2 (https=) |
| EP (1) | EP2173877B1 (https=) |
| JP (1) | JP5453261B2 (https=) |
| KR (1) | KR20100057016A (https=) |
| CN (1) | CN101835898B (https=) |
| AU (1) | AU2008279186B2 (https=) |
| CA (1) | CA2689099A1 (https=) |
| ES (1) | ES2439702T3 (https=) |
| IL (1) | IL203002A0 (https=) |
| WO (1) | WO2009015165A1 (https=) |
Families Citing this family (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9101687B2 (en) | 2002-07-15 | 2015-08-11 | University Of Leeds | Beta sheet tapes ribbons in tissue engineering |
| US8586539B2 (en) | 2002-07-15 | 2013-11-19 | University Of Leeds | Beta sheet tapes ribbons in tissue engineering |
| US7951559B2 (en) * | 2007-07-25 | 2011-05-31 | E.I. Du Pont De Nemours And Company | Recombinant peptide production using a cross-linkable solubility tag |
| US7829311B2 (en) * | 2007-07-25 | 2010-11-09 | E.I. Du Pont De Nemours And Company | Ketosteroid isomerase inclusion body tag engineered to be acid-resistant by replacing aspartates with glutamate |
| US7915011B2 (en) * | 2009-03-05 | 2011-03-29 | E.I. Du Pont De Nemours And Company | Host cell modifications that improve peptide production and downstream processing |
| US9241987B2 (en) | 2009-11-20 | 2016-01-26 | The University Of Chicago | Methods and compositions related to immunogenic fibrils |
| US8609621B2 (en) | 2010-11-15 | 2013-12-17 | E I Du Pont De Nemours And Company | Acid-cleavable linkers exhibiting altered rates of acid hydrolysis |
| US9273336B2 (en) | 2011-02-21 | 2016-03-01 | E. I. Du Pont De Nemours And Company | Recombinant host cells having an increase in buoyant density |
| US9115182B2 (en) * | 2011-06-21 | 2015-08-25 | E I Du Pont De Nemours And Company | Cysteine cross-linked structural peptides |
| US9062312B2 (en) * | 2011-06-21 | 2015-06-23 | Danisco Us Inc. | Fusion peptides comprising multi-functional peptidic solubility tags for efficient production, processing and surface applications |
| WO2014056199A1 (zh) * | 2012-10-12 | 2014-04-17 | 清华大学 | 多肽的产生和纯化方法 |
| GB201306338D0 (en) | 2013-04-08 | 2013-05-22 | Univ Leeds | Novel proteoglycans |
| CN104513298B (zh) * | 2014-12-09 | 2018-07-17 | 山东信得科技股份有限公司 | 一种表达鸡传染性法氏囊可溶性vp 2-4-3多肽的方法 |
| JP6640226B2 (ja) * | 2014-12-29 | 2020-02-05 | アグリカルチュラル テクノロジー リサーチ インスティテュート | 発現エレメント、発現カセット、及びそれらを含むベクター |
| GB2540763A (en) * | 2015-07-24 | 2017-02-01 | Evocatal Gmbh | Catalytically active protein aggregates and methods for producing the same |
| JP7623006B2 (ja) * | 2018-09-11 | 2025-01-28 | ナノタグ バイオテクノロジーズ ゲーエムベーハー | 特異的結合剤により認識されるエピトープタグ |
| US20250258180A1 (en) * | 2021-07-27 | 2025-08-14 | Stand Therapeutics Co., Ltd. | Peptide tag and nucleic acid encoding same |
| US20250042946A1 (en) | 2021-12-08 | 2025-02-06 | Oakgrove Bio Llc | Biological Production of Histidine-Rich Peptides |
| EP4567108A1 (en) * | 2023-12-05 | 2025-06-11 | Kutzner, Christoph | Screening of target peptides using fusion polypeptides |
Family Cites Families (54)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US516362A (en) * | 1894-03-13 | Retarding device for bolt-actuating mechanism | ||
| US641273A (en) * | 1899-06-03 | 1900-01-16 | John Dennis Coon | Cooking utensil. |
| US641936A (en) * | 1899-08-09 | 1900-01-23 | Edwin R Childs | Latticed or leaded glass. |
| US935642A (en) * | 1908-01-09 | 1909-10-05 | Gerhard Coenen | Flushing apparatus. |
| US5206154A (en) * | 1985-01-28 | 1993-04-27 | Xoma Corporation | Method of producing cecropins by microbiological techniques |
| WO1988007086A1 (en) * | 1987-03-20 | 1988-09-22 | Creative Biomolecules, Inc. | Leader sequences for the production of recombinant proteins |
| US5215896A (en) * | 1987-03-20 | 1993-06-01 | Creative Biomolecules, Inc. | Leader sequences for the production of recombinant proteins |
| US5223409A (en) * | 1988-09-02 | 1993-06-29 | Protein Engineering Corp. | Directed evolution of novel binding proteins |
| AU638762B2 (en) * | 1989-10-05 | 1993-07-08 | Optein Inc | Cell-free synthesis and isolation of novel genes and polypeptides |
| US5449754A (en) * | 1991-08-07 | 1995-09-12 | H & N Instruments, Inc. | Generation of combinatorial libraries |
| US5639603A (en) * | 1991-09-18 | 1997-06-17 | Affymax Technologies N.V. | Synthesizing and screening molecular diversity |
| ATE233817T1 (de) | 1992-08-21 | 2003-03-15 | Univ British Columbia | Kationische peptide und verfahren zu deren herstellung |
| US5585275A (en) * | 1992-09-02 | 1996-12-17 | Arris Pharmaceutical Corporation | Pilot apparatus for peptide synthesis and screening |
| US5480971A (en) * | 1993-06-17 | 1996-01-02 | Houghten Pharmaceuticals, Inc. | Peralkylated oligopeptide mixtures |
| US5648244A (en) * | 1993-09-27 | 1997-07-15 | President And Fellows Of Harvard College | Production, purification, cleavage and use of fusion peptides |
| US6037145A (en) * | 1994-09-07 | 2000-03-14 | Suntory Limited | Process for production of protein |
| ATE332368T1 (de) * | 1997-01-21 | 2006-07-15 | Gen Hospital Corp | Selektion von proteinen mittels rns-protein fusionen |
| US6261804B1 (en) * | 1997-01-21 | 2001-07-17 | The General Hospital Corporation | Selection of proteins using RNA-protein fusions |
| US6281018B1 (en) * | 1998-02-26 | 2001-08-28 | Coulter International Corp. | Selective purification and enrichment sorting of flow cytometer droplets based upon analysis of droplet precursor regions |
| US6846655B1 (en) * | 1998-06-29 | 2005-01-25 | Phylos, Inc. | Methods for generating highly diverse libraries |
| US6613548B1 (en) * | 1998-07-31 | 2003-09-02 | Pierce Biotechnology, Inc. | Fusion products containing insoluble proteinaceous tag |
| JP2002522091A (ja) * | 1998-08-17 | 2002-07-23 | フィロス インク． | ３’非翻訳領域を欠損する核酸を生成して細胞ｒｎａ蛋白質融合体形成を最適化する方法 |
| JP4278872B2 (ja) * | 1998-08-17 | 2009-06-17 | フィロス インク． | 蛋白質・核酸融合分子ライブラリーを用いた化合物・蛋白質相互作用の同定 |
| US6696089B2 (en) | 1998-09-03 | 2004-02-24 | Board Of Regents Of The University Of Nebraska | Nanogel networks including polyion polymer fragments and biological agent compositions thereof |
| FR2783169B1 (fr) * | 1998-09-15 | 2001-11-02 | Sederma Sa | Utilisation cosmetique ou dermopharmaceutique de peptides pour la cicatrisation et pour l'amelioration de l'aspect cutane lors du vieillissement naturel ou accelere (heliodermie, pollution) |
| NZ511699A (en) * | 1998-12-02 | 2003-02-28 | Phylos Inc | DNA-protein fusions and uses thereof |
| US6242219B1 (en) * | 1999-03-18 | 2001-06-05 | Xoma (Us) Llc | Methods for recombinant peptide production |
| DK1196637T3 (da) * | 1999-07-27 | 2007-07-30 | Adnexus Therapeutics Inc | Peptidacceptor-ligeringsmetoder |
| US6436665B1 (en) * | 1999-08-27 | 2002-08-20 | Phylos, Inc | Methods for encoding and sorting in vitro translated proteins |
| CA2402452A1 (en) * | 2000-03-31 | 2001-10-11 | Cambridge Antibody Technology Limited | Improvements to ribosome display |
| US20020098524A1 (en) | 2000-04-14 | 2002-07-25 | Murray Christopher J. | Methods for selective targeting |
| US7129326B2 (en) | 2000-04-14 | 2006-10-31 | Genencor International, Inc. | Methods for selective targeting |
| GB0117011D0 (en) * | 2001-07-12 | 2001-09-05 | Univ Leeds | Peptide barrels |
| AU2002367566B2 (en) * | 2001-11-20 | 2008-10-16 | Duke University | Interfacial biomaterials |
| CA2485835C (en) * | 2002-05-24 | 2018-03-06 | Restoragen, Inc. | Methods and dna constructs for high yield production of polypeptides |
| WO2003100022A2 (en) * | 2002-05-24 | 2003-12-04 | Restoragen, Inc. | Methods and dna constructs for high yield production of polypeptides |
| DE10227238A1 (de) | 2002-06-19 | 2004-01-15 | Wella Ag | Hochaffine kosmetische Mittel |
| GB0216286D0 (en) * | 2002-07-15 | 2002-08-21 | Univ Leeds | Network |
| EP2581383A1 (en) | 2002-11-25 | 2013-04-17 | Genencor International, Inc. | Skin or hair binding peptides |
| AU2003299869A1 (en) | 2002-12-26 | 2004-07-29 | Gendron, Inc. | Bariatric patient management system |
| US7220405B2 (en) | 2003-09-08 | 2007-05-22 | E. I. Du Pont De Nemours And Company | Peptide-based conditioners and colorants for hair, skin, and nails |
| US20050054752A1 (en) | 2003-09-08 | 2005-03-10 | O'brien John P. | Peptide-based diblock and triblock dispersants and diblock polymers |
| US7309482B2 (en) | 2003-09-08 | 2007-12-18 | E.I. Du Pont De Nemours And Company | Long lasting waterproof sunscreen comprising metal oxide and peptide conditioner |
| US7285264B2 (en) | 2003-09-08 | 2007-10-23 | E.I. Du Pont De Nemours And Company | Peptide-based body surface coloring reagents |
| US20050226839A1 (en) | 2003-09-08 | 2005-10-13 | Xueying Huang | Pepetide-based body surface reagents for personal care |
| US7736633B2 (en) | 2005-09-28 | 2010-06-15 | E.I. Du Pont De Nemours And Company | Method for enhancing effects of colorants and conditioners |
| US7928076B2 (en) | 2005-12-15 | 2011-04-19 | E. I. Du Pont De Nemours And Company | Polypropylene binding peptides and methods of use |
| US7906617B2 (en) | 2005-12-15 | 2011-03-15 | E. I. Du Pont De Nemours And Company | Polyethylene binding peptides and methods of use |
| US7632919B2 (en) | 2005-12-15 | 2009-12-15 | E.I. Du Pont De Nemours And Company | Polystyrene binding peptides and methods of use |
| US7709601B2 (en) | 2005-12-15 | 2010-05-04 | E. I. Du Pont De Nemours And Company | Nylon binding peptides and methods of use |
| US7700716B2 (en) | 2005-12-15 | 2010-04-20 | E. I. Du Pont De Nemours And Company | Polytetrafluoroethylene binding peptides and methods of use |
| US7749957B2 (en) | 2006-04-06 | 2010-07-06 | E.I. Du Pont De Nemours And Company | Clay-binding peptides and methods of use |
| US7732569B2 (en) | 2006-10-19 | 2010-06-08 | E.I. Du Pont De Nemours And Company | Zein-based peptide tags for the expression and purification of bioactive peptides |
| US7662913B2 (en) | 2006-10-19 | 2010-02-16 | E. I. Du Pont De Nemours And Company | Cystatin-based peptide tags for the expression and purification of bioactive peptides |
-
2007
- 2007-07-25 US US11/782,836 patent/US7678883B2/en not_active Expired - Fee Related
-
2008
- 2008-07-23 EP EP08782229.2A patent/EP2173877B1/en not_active Not-in-force
- 2008-07-23 AU AU2008279186A patent/AU2008279186B2/en not_active Ceased
- 2008-07-23 CA CA002689099A patent/CA2689099A1/en not_active Abandoned
- 2008-07-23 JP JP2010518341A patent/JP5453261B2/ja not_active Expired - Fee Related
- 2008-07-23 ES ES08782229.2T patent/ES2439702T3/es active Active
- 2008-07-23 KR KR1020107004040A patent/KR20100057016A/ko not_active Ceased
- 2008-07-23 WO PCT/US2008/070802 patent/WO2009015165A1/en not_active Ceased
- 2008-07-23 CN CN2008800253760A patent/CN101835898B/zh not_active Expired - Fee Related
-
2009
- 2009-12-27 IL IL203002A patent/IL203002A0/en unknown
-
2010
- 2010-01-25 US US12/692,665 patent/US7794979B2/en not_active Expired - Fee Related
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