JP2010518857A - 分化した細胞の再プログラムおよび再プログラムされた細胞からの動物および胚幹細胞の生成のための高能率的な方法 - Google Patents

分化した細胞の再プログラムおよび再プログラムされた細胞からの動物および胚幹細胞の生成のための高能率的な方法 Download PDF

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JP2010518857A
JP2010518857A JP2009550931A JP2009550931A JP2010518857A JP 2010518857 A JP2010518857 A JP 2010518857A JP 2009550931 A JP2009550931 A JP 2009550931A JP 2009550931 A JP2009550931 A JP 2009550931A JP 2010518857 A JP2010518857 A JP 2010518857A
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ロバート ランサ，
ヤン チャン，
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アドバンスド セル テクノロジー， インコーポレイテッド
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JP2009550931A 2007-02-23 2008-02-22 分化した細胞の再プログラムおよび再プログラムされた細胞からの動物および胚幹細胞の生成のための高能率的な方法 Pending JP2010518857A (ja)

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Application Number Priority Date Filing Date Title
US90297007P 2007-02-23 2007-02-23
US91854307P 2007-03-16 2007-03-16
US99377207P 2007-09-14 2007-09-14
US943207P 2007-12-28 2007-12-28
PCT/US2008/002380 WO2008103462A2 (en) 2007-02-23 2008-02-22 Highly efficient methods for reprogramming differentiated cells and for generating animals and embryonic stem cells from reprogrammed cells

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JP2013097811A Division JP5955809B2 (ja) 2007-02-23 2013-05-07 分化した細胞の再プログラムおよび再プログラムされた細胞からの動物および胚幹細胞の生成のための高能率的な方法

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JP2010518857A5 JP2010518857A5 (enExample) 2010-10-14

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JP2009550931A Pending JP2010518857A (ja) 2007-02-23 2008-02-22 分化した細胞の再プログラムおよび再プログラムされた細胞からの動物および胚幹細胞の生成のための高能率的な方法
JP2013097811A Active JP5955809B2 (ja) 2007-02-23 2013-05-07 分化した細胞の再プログラムおよび再プログラムされた細胞からの動物および胚幹細胞の生成のための高能率的な方法
JP2016119245A Active JP6423386B2 (ja) 2007-02-23 2016-06-15 分化した細胞の再プログラムおよび再プログラムされた細胞からの動物および胚幹細胞の生成のための高能率的な方法
JP2018196337A Pending JP2019004912A (ja) 2007-02-23 2018-10-18 分化した細胞の再プログラムおよび再プログラムされた細胞からの動物および胚幹細胞の生成のための高能率的な方法

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JP2013097811A Active JP5955809B2 (ja) 2007-02-23 2013-05-07 分化した細胞の再プログラムおよび再プログラムされた細胞からの動物および胚幹細胞の生成のための高能率的な方法
JP2016119245A Active JP6423386B2 (ja) 2007-02-23 2016-06-15 分化した細胞の再プログラムおよび再プログラムされた細胞からの動物および胚幹細胞の生成のための高能率的な方法
JP2018196337A Pending JP2019004912A (ja) 2007-02-23 2018-10-18 分化した細胞の再プログラムおよび再プログラムされた細胞からの動物および胚幹細胞の生成のための高能率的な方法

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8796021B2 (en) 2007-02-23 2014-08-05 Advanced Cell Technology, Inc. Blastomere culture to produce mammalian embryonic stem cells
JP2021514199A (ja) * 2018-01-17 2021-06-10 センター フォー エクセレンス イン ブレイン サイエンス アンド インテリジェンス テクノロジー，チャイニーズ アカデミー オブ サイエンシーズＣｅｎｔｅｒ Ｆｏｒ Ｅｘｃｅｌｌｅｎｃｅ Ｉｎ Ｂｒａｉｎ Ｓｃｉｅｎｃｅ Ａｎｄ Ｉｎｔｅｌｌｉｇｅｎｃｅ Ｔｅｃｈｎｏｌｏｇｙ，Ｃｈｉｎｅｓｅ Ａｃａｄｅｍｙ Ｏｆ Ｓｃｉｅｎｃｅｓ 非ヒト霊長類の体細胞クローン動物の製造方法
WO2022272042A3 (en) * 2021-06-25 2023-02-16 The J. David Gladstone Institutes, A Testamentary Trust Established Under The Will Of J. David Gladstone Primordial germ cells

Families Citing this family (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA3015835A1 (en) 2004-11-04 2006-05-18 Astellas Institute For Regenerative Medicine Derivation of embryonic stem cells
US7893315B2 (en) 2004-11-04 2011-02-22 Advanced Cell Technology, Inc. Derivation of embryonic stem cells and embryo-derived cells
CA2659945C (en) 2005-08-03 2014-12-16 Advanced Cell Technology, Inc. Improved methods of reprogramming animal somatic cells
EP2283118A4 (en) 2008-05-06 2012-11-21 Agency Science Tech & Res METHOD FOR LEARNING THE DEDIFFERENCING OF A CELL
CN101798569B (zh) * 2009-02-09 2012-11-21 北京瑞途之星科技有限公司 以卵裂后的发育胚胎替代卵母细胞的治疗性克隆新方法
AU2011235212B2 (en) * 2010-03-31 2014-07-31 The Scripps Research Institute Reprogramming cells
CN101921808B (zh) * 2010-08-18 2012-05-30 东北农业大学 一种提高猪体细胞核移植效率的方法
US20120184035A1 (en) * 2011-01-13 2012-07-19 Sadhana Agarwal Methods and Compositions For Reprogramming Cells
WO2012174086A1 (en) * 2011-06-14 2012-12-20 J. R. Simplot Company A method for identifying a mammal receptive to embryo transfer
WO2013010045A1 (en) 2011-07-12 2013-01-17 Biotime Inc. Novel methods and formulations for orthopedic cell therapy
CN102321571A (zh) * 2011-09-21 2012-01-18 王跃嗣 从分化细胞制备自体造血干细胞的方法
KR102108245B1 (ko) 2011-11-30 2020-05-08 아스텔라스 인스티튜트 포 리제너러티브 메디슨 중간엽 간질 세포 및 이에 관련된 용도
US8961956B2 (en) 2011-11-30 2015-02-24 Ocata Therapeutics, Inc. Mesenchymal stromal cells and uses related thereto
US9428732B2 (en) * 2011-12-05 2016-08-30 Primorigen Biosciences, Inc. Compositions and methods for differentiating pluripotent stem cells into primitive blood cells and uses thereof
US9179935B2 (en) * 2012-01-13 2015-11-10 The Board Of Trustees Of The Leland Stanford Junior University Mechanical biomarkers for oocyte and embryo viability
CN102608323A (zh) * 2012-03-26 2012-07-25 西北农林科技大学 一种基于哺乳动物囊胚的分子免疫检测的胚胎质量分析方法
CN104471059B (zh) 2012-07-12 2018-04-17 珠海横琴爱姆斯坦生物科技有限公司 人胚胎干细胞衍生的间充质样干细胞、方法及其应用
WO2014144754A1 (en) 2013-03-15 2014-09-18 The New York Stem Cell Foundation Somatic cell nuclear transfer methods
JP6689498B2 (ja) * 2013-04-16 2020-04-28 イーエムべーアー−インスティツート フューア モレクラー ビオテクノロギー ゲーエムベーハー ブラストイド、細胞株に基づく人工胚盤胞
JP2016073207A (ja) * 2014-10-02 2016-05-12 株式会社リプロセル 細胞凍結保存方法、細胞解凍方法、及び細胞
EP4343331A3 (en) 2015-03-23 2024-06-19 Astellas Institute for Regenerative Medicine Improved assays for potency of human retinal pigment epithelium (rpe) cells and photoreceptor progenitors
CA3019574C (en) * 2016-03-31 2025-09-23 Sanbio Inc Medium, methods, cells and secreted factors for stem cell culture and therapy
US20180127738A1 (en) * 2016-11-07 2018-05-10 BiomediStem, LLC Production and therapeutic uses of epinul pluripotent cells and differentiated cells derived therefrom
EP3824906A1 (en) 2016-12-21 2021-05-26 Amgen Inc. Anti-tnf alpha antibody formulations
CN107099553A (zh) * 2017-06-19 2017-08-29 内蒙古大学 一种小鼠体细胞核移植方法
CA3076500C (en) * 2017-09-21 2025-07-08 Emulate Inc REOLOGICALLY BIOMIMITED FLUID SUBSTITUTE
CN110819585B (zh) * 2018-08-09 2022-12-06 山东大学 含有igf2的胚胎体外培育方法和培养基
TWI715874B (zh) * 2018-09-27 2021-01-11 台灣粒線體應用技術股份有限公司 無血清培養液以及使用此無血清培養液分離和放大培養幹細胞方法
EP3870188B1 (en) * 2018-10-23 2024-06-19 The Board of Trustees of the Leland Stanford Junior University Prevention and treatment of teratoma formation in stem cell-based thereapies using alternating electric fields
CN113925878B (zh) * 2021-10-20 2024-03-08 上海交通大学医学院附属仁济医院 一种细胞外囊泡在治疗神经损伤性勃起功能障碍中的应用
CN114128668A (zh) * 2021-11-24 2022-03-04 华中农业大学 一种提高水牛夏季同期发情-定时输精效果的处理方法
WO2025206342A1 (ja) * 2024-03-29 2025-10-02 学校法人順天堂 組織幹細胞の老化抑制剤
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CN119639658B (zh) * 2025-02-18 2025-07-15 中国农业大学 促进胚胎体外发育的组合物、培养液、产品及方法
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Family Cites Families (63)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4441972A (en) 1983-04-08 1984-04-10 D.E.P. Systems, Inc. Apparatus for electrofusion of biological particles
US4664097A (en) 1984-05-09 1987-05-12 The Wistar Institute Of Anatomy & Biology Nuclear transplantation in the mammalian embryo by microsurgery and cell fusion
US4578168A (en) 1984-07-27 1986-03-25 Biotronics Apparatus for fusing live cells with electric fields
US4994384A (en) 1986-12-31 1991-02-19 W. R. Grace & Co.-Conn. Multiplying bovine embryos
US5057420A (en) 1987-06-05 1991-10-15 Granada Biosciences, Inc. Bovine nuclear transplantation
US5213979A (en) 1987-12-30 1993-05-25 W. R. Grace & Co.-Conn. In vitro culture of bovine embryos
EP0435928A1 (en) 1988-09-21 1991-07-10 Animal Biotechnology Cambridge Limited Derivation of pluripotential embryonic cell lines from domestic animals
US4944384A (en) 1989-01-30 1990-07-31 Hay & Forage Industries Trash discharge apparatus for crop transferring conveyor mechanism
CA2086330A1 (en) 1990-07-09 1992-01-10 Richard C. Fry Enhanced implantation, development and maintenance of embryos using leukaemia inhibitory factor
US5096822A (en) 1990-07-26 1992-03-17 W. R. Grace & Co.- Conn. Bovine embryo medium
JP3104995B2 (ja) 1991-01-31 2000-10-30 ワーナー−ランバート・コンパニー 抗炎症剤として有用な４，６−ジ−第三ブチル−５−ヒドロキシ−１，３−ピリミジンの置換されたヘテロアリール類似体
US5173158A (en) 1991-07-22 1992-12-22 Schmukler Robert E Apparatus and methods for electroporation and electrofusion
US5496720A (en) 1993-02-10 1996-03-05 Susko-Parrish; Joan L. Parthenogenic oocyte activation
GB9308271D0 (en) 1993-04-21 1993-06-02 Univ Edinburgh Method of isolating and/or enriching and/or selectively propagating pluripotential animal cells and animals for use in said method
US5523226A (en) 1993-05-14 1996-06-04 Biotechnology Research And Development Corp. Transgenic swine compositions and methods
AU1439295A (en) 1993-12-17 1995-07-03 Abs Global, Inc. Ungulate preblastocyst derived embryonic stem cells and use thereof to produce cloned transgenic and chimeric ungulates
EP0739412B1 (en) 1993-12-23 2002-02-27 Infigen, Inc. ungulate EMBRYONIC STEM CELLS AS NUCLEAR DONORS AND NUCLEAR TRANSFER TECHNIQUES TO PRODUCE CHIMERIC AND TRANSGENIC ANIMALS
US5843780A (en) 1995-01-20 1998-12-01 Wisconsin Alumni Research Foundation Primate embryonic stem cells
US7696404B2 (en) * 1996-08-19 2010-04-13 Advanced Cell Technology, Inc. Embryonic or stem-like cell lines produced by cross species nuclear transplantation and methods for enhancing embryonic development by genetic alteration of donor cells or by tissue culture conditions
US20090092588A1 (en) * 1997-01-10 2009-04-09 University Of Massachusetts As Represented By Its Amherst Campus Cloned ungulate embryos and animals, use of cells, tissues and organs thereof for transplantation therapies including parkinson's disease
US5945577A (en) 1997-01-10 1999-08-31 University Of Massachusetts As Represented By Its Amherst Campus Cloning using donor nuclei from proliferating somatic cells
NZ337495A (en) 1997-03-06 2001-06-29 Infigen Inc Bovine primordial germ cells and their use in cloning
US6011197A (en) * 1997-03-06 2000-01-04 Infigen, Inc. Method of cloning bovines using reprogrammed non-embryonic bovine cells
US6667176B1 (en) * 2000-01-11 2003-12-23 Geron Corporation cDNA libraries reflecting gene expression during growth and differentiation of human pluripotent stem cells
US6372494B1 (en) * 1999-05-14 2002-04-16 Advanced Tissue Sciences, Inc. Methods of making conditioned cell culture medium compositions
US20040199935A1 (en) 1999-06-30 2004-10-07 Chapman Karen B. Cytoplasmic transfer to de-differentiate recipient cells
EP1196426A4 (en) * 1999-06-30 2003-09-03 Advanced Cell Tech Inc CYTOPLASMATIC TRANSFER FOR DE-DIFFERENTIATION OF VASCELL CELLS
CA2387488A1 (en) 1999-10-14 2001-04-19 James M. Robl Preparation and selection of donor cells for nuclear transplantation
BR0014864A (pt) 1999-10-15 2002-11-19 Advanced Cell Tech Inc Métodos de produzir células progenitoras diferenciadas e células tronco embriÈnicas defeituosas em linhagem
CN1425064A (zh) 1999-12-20 2003-06-18 马萨诸塞大学 通过交叉物种核移植制备的胚胎或干样细胞
CA2394812A1 (en) 1999-12-20 2001-06-28 Anthony C.F. Perry A method to produce cloned embryos and adults from cultured cells
WO2001050848A2 (en) 2000-01-07 2001-07-19 Oregon Health And Science University Clonal propagation of primate offspring by embryo splitting
DE10108412B4 (de) * 2001-02-21 2006-03-09 Cevec Pharmaceuticals Gmbh Pigmentepithelzelle des Auges, ihre Herstellung und Verwendung in der Therapie einer Augen- oder Nervenerkrankung
IL160507A0 (en) 2001-08-24 2004-07-25 Advanced Cell Tech Inc Screening assays for identifying differentiation-inducing agents and production of differentiated cells for cell therapy
US20030044976A1 (en) * 2001-08-27 2003-03-06 Advanced Cell Technology De-differentiation and re-differentiation of somatic cells and production of cells for cell therapies
JP2003067271A (ja) * 2001-08-27 2003-03-07 Hitachi Ltd 統合管理システム
WO2003018767A2 (en) 2001-08-27 2003-03-06 Advanced Cell Technology, Inc. Trans-differentiation and re-differentiation of somatic cells and production of cells for cell therapies
WO2003046141A2 (en) 2001-11-26 2003-06-05 Advanced Cell Technology, Inc. Methods for making and using reprogrammed human somatic cell nuclei and autologous and isogenic human stem cells
WO2003057836A2 (en) 2001-12-27 2003-07-17 Advanced Cell Technology, Inc. Embryonic or stem-like cell lines produced by cross species nuclear transplantation and methods for enhancing embryonic development by genetic alteration of donor cells or by tissue culture conditions
US7247477B2 (en) 2002-04-16 2007-07-24 Technion Research & Development Foundation Ltd. Methods for the in-vitro identification, isolation and differentiation of vasculogenic progenitor cells
AU2003233679A1 (en) 2002-05-23 2003-12-12 Advanced Cell Technology, Inc. Generation of histocompatible tissues using nuclear transplantation
US7612250B2 (en) 2002-07-29 2009-11-03 Trustees Of Tufts College Nuclear transfer embryo formation method
JP4374419B2 (ja) 2002-10-31 2009-12-02 独立行政法人理化学研究所 多能性幹細胞培養用の組成物とその使用
AU2003296480A1 (en) * 2002-12-10 2004-06-30 The Wistar Institute Method for programmed differentiation of stem cells
EP2457999B1 (en) 2002-12-16 2018-10-17 Technion Research & Development Foundation Ltd. Culture medium for pluripotent stem cells
US20040229350A1 (en) * 2003-05-12 2004-11-18 Nikolai Strelchenko Morula derived embryonic stem cells
US20050118713A1 (en) * 2003-05-12 2005-06-02 Nikolai Strelchenko Morula derived embryonic stem cells
TW200521236A (en) * 2003-12-22 2005-07-01 Animal Technology Inst Taiwan Method for generating non-human mammalian chimeric embryo
SG188122A1 (en) 2004-01-23 2013-03-28 Advanced Cell Tech Inc Improved modalities for the treatment of degenerative diseases of the retina
US7794704B2 (en) 2004-01-23 2010-09-14 Advanced Cell Technology, Inc. Methods for producing enriched populations of human retinal pigment epithelium cells for treatment of retinal degeneration
WO2005080551A2 (en) 2004-02-12 2005-09-01 University Of Newcastle Upon Tyne Stem cells
WO2006013519A1 (en) 2004-07-26 2006-02-09 Koninklijke Philips Electronics N.V. Method of storing data in an information carrier, system for reading such an information carrier.
US20080262321A1 (en) 2004-08-06 2008-10-23 Ramot At Tel Aviv University Ltd. Early Detection of Harmful Agents: Method, System and Kit
CA3015835A1 (en) * 2004-11-04 2006-05-18 Astellas Institute For Regenerative Medicine Derivation of embryonic stem cells
US7893315B2 (en) 2004-11-04 2011-02-22 Advanced Cell Technology, Inc. Derivation of embryonic stem cells and embryo-derived cells
CA2659945C (en) 2005-08-03 2014-12-16 Advanced Cell Technology, Inc. Improved methods of reprogramming animal somatic cells
WO2007047894A2 (en) 2005-10-20 2007-04-26 Advanced Cell Technology, Inc. Totipotent, nearly totipotent or pluripotent mammalian cells homozygous or hemizygous for one or more histocompatibility antigen genes
US20080070303A1 (en) 2005-11-21 2008-03-20 West Michael D Methods to accelerate the isolation of novel cell strains from pluripotent stem cells and cells obtained thereby
WO2007058671A1 (en) 2005-11-21 2007-05-24 West Michael D Novel uses of cells with prenatal patterns of gene expression
KR101602951B1 (ko) 2006-05-03 2016-03-14 오카타 세라퓨틱스, 인크. 배아 줄기 세포 및 배-유도 세포의 유도
CN100465268C (zh) * 2006-05-17 2009-03-04 北京大学 人胚胎干细胞的培养方法及其专用培养基
US20070298496A1 (en) * 2006-06-23 2007-12-27 Hung-Chih Kuo Method of deriving pluripotent stem cells from a single blastomere
EP3190178A1 (en) 2007-02-23 2017-07-12 Astellas Institute for Regenerative Medicine Highly efficient methods for reprogramming differentiated cells and for generating animals and embryonic stem cells from reprogrammed cells

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
JPN6012068469; 総合科学技術会議: ヒト胚の取扱いに関する基本的考え方 , 20040723, ｐ．１-２０ *
JPN6013028234; 「ヒトに関するクローン技術等の規制に関する法律」解説資料 , 20130530 *

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8796021B2 (en) 2007-02-23 2014-08-05 Advanced Cell Technology, Inc. Blastomere culture to produce mammalian embryonic stem cells
US10584313B2 (en) 2007-02-23 2020-03-10 Astellas Institute For Regenerative Medicine Method of producing a differentiated mammalian cell comprising culturing a single mammalian blastomere
JP2021514199A (ja) * 2018-01-17 2021-06-10 センター フォー エクセレンス イン ブレイン サイエンス アンド インテリジェンス テクノロジー，チャイニーズ アカデミー オブ サイエンシーズＣｅｎｔｅｒ Ｆｏｒ Ｅｘｃｅｌｌｅｎｃｅ Ｉｎ Ｂｒａｉｎ Ｓｃｉｅｎｃｅ Ａｎｄ Ｉｎｔｅｌｌｉｇｅｎｃｅ Ｔｅｃｈｎｏｌｏｇｙ，Ｃｈｉｎｅｓｅ Ａｃａｄｅｍｙ Ｏｆ Ｓｃｉｅｎｃｅｓ 非ヒト霊長類の体細胞クローン動物の製造方法
JP7199741B2 (ja) 2018-01-17 2023-01-06 センター フォー エクセレンス イン ブレイン サイエンス アンド インテリジェンス テクノロジー，チャイニーズ アカデミー オブ サイエンシーズ 非ヒト霊長類の体細胞クローン動物の製造方法
WO2022272042A3 (en) * 2021-06-25 2023-02-16 The J. David Gladstone Institutes, A Testamentary Trust Established Under The Will Of J. David Gladstone Primordial germ cells
JP2024522861A (ja) * 2021-06-25 2024-06-21 ザ ジェイ． デビッド グラッドストーン インスティテューツ、 ア テスタメンタリー トラスト エスタブリッシュド アンダー ザ ウィル オブ ジェイ． デビッド グラッドストーン 始原生殖細胞

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