JP2010507363A5 - - Google Patents
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- Publication number
- JP2010507363A5 JP2010507363A5 JP2009533438A JP2009533438A JP2010507363A5 JP 2010507363 A5 JP2010507363 A5 JP 2010507363A5 JP 2009533438 A JP2009533438 A JP 2009533438A JP 2009533438 A JP2009533438 A JP 2009533438A JP 2010507363 A5 JP2010507363 A5 JP 2010507363A5
- Authority
- JP
- Japan
- Prior art keywords
- acid
- virus
- vero cells
- cell culture
- supernatant
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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- 238000000034 method Methods 0.000 claims description 47
- 241000700605 Viruses Species 0.000 claims description 41
- IPCSVZSSVZVIGE-UHFFFAOYSA-N hexadecanoic acid Chemical compound CCCCCCCCCCCCCCCC(O)=O IPCSVZSSVZVIGE-UHFFFAOYSA-N 0.000 claims description 19
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 18
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 claims description 18
- SECPZKHBENQXJG-FPLPWBNLSA-N palmitoleic acid Chemical compound CCCCCC\C=C/CCCCCCCC(O)=O SECPZKHBENQXJG-FPLPWBNLSA-N 0.000 claims description 16
- 210000003501 vero cell Anatomy 0.000 claims description 13
- 150000002632 lipids Chemical class 0.000 claims description 11
- 239000006143 cell culture medium Substances 0.000 claims description 10
- 239000012141 concentrate Substances 0.000 claims description 10
- OYHQOLUKZRVURQ-NTGFUMLPSA-N (9Z,12Z)-9,10,12,13-tetratritiooctadeca-9,12-dienoic acid Chemical compound C(CCCCCCC\C(=C(/C\C(=C(/CCCCC)\[3H])\[3H])\[3H])\[3H])(=O)O OYHQOLUKZRVURQ-NTGFUMLPSA-N 0.000 claims description 9
- WRIDQFICGBMAFQ-UHFFFAOYSA-N (E)-8-Octadecenoic acid Natural products CCCCCCCCCC=CCCCCCCC(O)=O WRIDQFICGBMAFQ-UHFFFAOYSA-N 0.000 claims description 9
- LQJBNNIYVWPHFW-UHFFFAOYSA-N 20:1omega9c fatty acid Natural products CCCCCCCCCCC=CCCCCCCCC(O)=O LQJBNNIYVWPHFW-UHFFFAOYSA-N 0.000 claims description 9
- QSBYPNXLFMSGKH-UHFFFAOYSA-N 9-Heptadecensaeure Natural products CCCCCCCC=CCCCCCCCC(O)=O QSBYPNXLFMSGKH-UHFFFAOYSA-N 0.000 claims description 9
- ZQPPMHVWECSIRJ-UHFFFAOYSA-N Oleic acid Natural products CCCCCCCCC=CCCCCCCCC(O)=O ZQPPMHVWECSIRJ-UHFFFAOYSA-N 0.000 claims description 9
- 239000005642 Oleic acid Substances 0.000 claims description 9
- 235000021314 Palmitic acid Nutrition 0.000 claims description 9
- 235000021355 Stearic acid Nutrition 0.000 claims description 9
- DTOSIQBPPRVQHS-PDBXOOCHSA-N alpha-linolenic acid Chemical compound CC\C=C/C\C=C/C\C=C/CCCCCCCC(O)=O DTOSIQBPPRVQHS-PDBXOOCHSA-N 0.000 claims description 9
- 235000020661 alpha-linolenic acid Nutrition 0.000 claims description 9
- 235000012000 cholesterol Nutrition 0.000 claims description 9
- 235000019441 ethanol Nutrition 0.000 claims description 9
- QXJSBBXBKPUZAA-UHFFFAOYSA-N isooleic acid Natural products CCCCCCCC=CCCCCCCCCC(O)=O QXJSBBXBKPUZAA-UHFFFAOYSA-N 0.000 claims description 9
- 229960004488 linolenic acid Drugs 0.000 claims description 9
- KQQKGWQCNNTQJW-UHFFFAOYSA-N linolenic acid Natural products CC=CCCC=CCC=CCCCCCCCC(O)=O KQQKGWQCNNTQJW-UHFFFAOYSA-N 0.000 claims description 9
- WQEPLUUGTLDZJY-UHFFFAOYSA-N n-Pentadecanoic acid Natural products CCCCCCCCCCCCCCC(O)=O WQEPLUUGTLDZJY-UHFFFAOYSA-N 0.000 claims description 9
- QIQXTHQIDYTFRH-UHFFFAOYSA-N octadecanoic acid Chemical compound CCCCCCCCCCCCCCCCCC(O)=O QIQXTHQIDYTFRH-UHFFFAOYSA-N 0.000 claims description 9
- OQCDKBAXFALNLD-UHFFFAOYSA-N octadecanoic acid Natural products CCCCCCCC(C)CCCCCCCCC(O)=O OQCDKBAXFALNLD-UHFFFAOYSA-N 0.000 claims description 9
- ZQPPMHVWECSIRJ-KTKRTIGZSA-N oleic acid Chemical compound CCCCCCCC\C=C/CCCCCCCC(O)=O ZQPPMHVWECSIRJ-KTKRTIGZSA-N 0.000 claims description 9
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 claims description 9
- 229920000053 polysorbate 80 Polymers 0.000 claims description 9
- 239000008117 stearic acid Substances 0.000 claims description 9
- TUNFSRHWOTWDNC-HKGQFRNVSA-N tetradecanoic acid Chemical compound CCCCCCCCCCCCC[14C](O)=O TUNFSRHWOTWDNC-HKGQFRNVSA-N 0.000 claims description 9
- 235000021319 Palmitoleic acid Nutrition 0.000 claims description 8
- RVGRUAULSDPKGF-UHFFFAOYSA-N Poloxamer Chemical compound C1CO1.CC1CO1 RVGRUAULSDPKGF-UHFFFAOYSA-N 0.000 claims description 8
- SECPZKHBENQXJG-UHFFFAOYSA-N cis-palmitoleic acid Natural products CCCCCCC=CCCCCCCCC(O)=O SECPZKHBENQXJG-UHFFFAOYSA-N 0.000 claims description 8
- 229920001993 poloxamer 188 Polymers 0.000 claims description 8
- 239000000203 mixture Substances 0.000 claims description 7
- 238000004113 cell culture Methods 0.000 claims description 6
- 208000015181 infectious disease Diseases 0.000 claims description 4
- 238000012258 culturing Methods 0.000 claims description 3
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 claims description 2
- 239000011627 DL-alpha-tocopherol Substances 0.000 claims description 2
- 235000001815 DL-alpha-tocopherol Nutrition 0.000 claims description 2
- 239000002253 acid Substances 0.000 claims description 2
- GVJHHUAWPYXKBD-UHFFFAOYSA-N d-alpha-tocopherol Natural products OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 claims description 2
- 238000010899 nucleation Methods 0.000 claims description 2
- 230000001902 propagating effect Effects 0.000 claims description 2
- 229960000984 tocofersolan Drugs 0.000 claims description 2
- GVJHHUAWPYXKBD-IEOSBIPESA-N α-tocopherol Chemical compound OC1=C(C)C(C)=C2O[C@@](CCC[C@H](C)CCC[C@H](C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-IEOSBIPESA-N 0.000 claims description 2
- 239000006228 supernatant Substances 0.000 description 19
- YZXBAPSDXZZRGB-DOFZRALJSA-N arachidonic acid Chemical compound CCCCC\C=C/C\C=C/C\C=C/C\C=C/CCCC(O)=O YZXBAPSDXZZRGB-DOFZRALJSA-N 0.000 description 16
- 210000004027 cell Anatomy 0.000 description 10
- 208000002606 Paramyxoviridae Infections Diseases 0.000 description 8
- 229940114079 arachidonic acid Drugs 0.000 description 8
- 235000021342 arachidonic acid Nutrition 0.000 description 8
- 235000021313 oleic acid Nutrition 0.000 description 8
- ZAKOWWREFLAJOT-CEFNRUSXSA-N D-alpha-tocopherylacetate Chemical compound CC(=O)OC1=C(C)C(C)=C2O[C@@](CCC[C@H](C)CCC[C@H](C)CCCC(C)C)(C)CCC2=C1C ZAKOWWREFLAJOT-CEFNRUSXSA-N 0.000 description 7
- 239000011626 DL-alpha-tocopherylacetate Substances 0.000 description 7
- 235000001809 DL-alpha-tocopherylacetate Nutrition 0.000 description 7
- 210000002966 serum Anatomy 0.000 description 7
- 239000012679 serum free medium Substances 0.000 description 7
- 229940042585 tocopherol acetate Drugs 0.000 description 7
- 239000002609 medium Substances 0.000 description 6
- 241000712005 Bovine respirovirus 3 Species 0.000 description 4
- 241001493065 dsRNA viruses Species 0.000 description 4
- 238000013019 agitation Methods 0.000 description 3
- 239000002773 nucleotide Substances 0.000 description 3
- 125000003729 nucleotide group Chemical group 0.000 description 3
- 241000283690 Bos taurus Species 0.000 description 2
- 101710133291 Hemagglutinin-neuraminidase Proteins 0.000 description 2
- 241000351643 Metapneumovirus Species 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 241000725643 Respiratory syncytial virus Species 0.000 description 2
- 230000000241 respiratory effect Effects 0.000 description 2
- 108010068327 4-hydroxyphenylpyruvate dioxygenase Proteins 0.000 description 1
- 241000699800 Cricetinae Species 0.000 description 1
- 101150034814 F gene Proteins 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 108090000288 Glycoproteins Proteins 0.000 description 1
- JVTAAEKCZFNVCJ-UHFFFAOYSA-M Lactate Chemical compound CC(O)C([O-])=O JVTAAEKCZFNVCJ-UHFFFAOYSA-M 0.000 description 1
- 108091028043 Nucleic acid sequence Proteins 0.000 description 1
- 102000004142 Trypsin Human genes 0.000 description 1
- 108090000631 Trypsin Proteins 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- -1 ammonium ions Chemical class 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000002238 attenuated effect Effects 0.000 description 1
- 229940031416 bivalent vaccine Drugs 0.000 description 1
- 230000023555 blood coagulation Effects 0.000 description 1
- 239000012228 culture supernatant Substances 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000003472 neutralizing effect Effects 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
- 229960005486 vaccine Drugs 0.000 description 1
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US86255006P | 2006-10-23 | 2006-10-23 | |
| PCT/US2007/066037 WO2007118134A2 (en) | 2006-04-06 | 2007-04-05 | A serum-free virus propagation platform for a virus vaccine candidate |
| US94416207P | 2007-06-15 | 2007-06-15 | |
| US97392107P | 2007-09-20 | 2007-09-20 | |
| PCT/US2007/080610 WO2008051698A2 (en) | 2006-10-23 | 2007-10-05 | A serum-free virus propagation platform for a virus vaccine candidate |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2010507363A JP2010507363A (ja) | 2010-03-11 |
| JP2010507363A5 true JP2010507363A5 (enrdf_load_stackoverflow) | 2010-11-25 |
Family
ID=40691374
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2009533438A Pending JP2010507363A (ja) | 2006-10-23 | 2007-10-05 | ウイルスワクチン候補のための無血清ウイルス増殖プラットフォーム |
Country Status (5)
| Country | Link |
|---|---|
| EP (1) | EP2076603A4 (enrdf_load_stackoverflow) |
| JP (1) | JP2010507363A (enrdf_load_stackoverflow) |
| KR (1) | KR20090084859A (enrdf_load_stackoverflow) |
| BR (1) | BRPI0717469A2 (enrdf_load_stackoverflow) |
| WO (1) | WO2008051698A2 (enrdf_load_stackoverflow) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101875917B (zh) * | 2009-04-28 | 2012-07-04 | 北京清大天一科技有限公司 | 生物反应器微载体培养动物细胞生产猪瘟疫苗的方法 |
| JP2014513947A (ja) * | 2011-04-18 | 2014-06-19 | ザ・トラスティーズ・オブ・プリンストン・ユニバーシティ | 培養細胞においてウイルスを生産する方法 |
| JP6917984B2 (ja) * | 2016-05-09 | 2021-08-11 | 協和発酵バイオ株式会社 | 培地、アルブミン非含有培地用添加剤および多能性幹細胞の培養方法 |
| KR20200044973A (ko) * | 2017-09-15 | 2020-04-29 | 라이프 테크놀로지스 코포레이션 | 세포를 배양 및 팽창시키기 위한 조성물 및 방법 |
| WO2024075819A1 (ja) * | 2022-10-07 | 2024-04-11 | Ａｇｃ株式会社 | 細胞を用いたウイルスベクターの生産方法 |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2007118134A2 (en) * | 2006-04-06 | 2007-10-18 | Medimmune, Inc. | A serum-free virus propagation platform for a virus vaccine candidate |
-
2007
- 2007-10-05 EP EP07853807A patent/EP2076603A4/en not_active Withdrawn
- 2007-10-05 JP JP2009533438A patent/JP2010507363A/ja active Pending
- 2007-10-05 WO PCT/US2007/080610 patent/WO2008051698A2/en active Application Filing
- 2007-10-05 KR KR1020097009590A patent/KR20090084859A/ko not_active Ceased
- 2007-10-05 BR BRPI0717469-1A2A patent/BRPI0717469A2/pt not_active IP Right Cessation
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