JP2009225711A - 組換え微生物 - Google Patents
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Abstract
【解決手段】枯草菌遺伝子yvyD又は当該遺伝子に相当する遺伝子が欠失又は不活性化された微生物株に、異種のタンパク質又はポリペプチドをコードする遺伝子を導入した組換え微生物。
【選択図】なし
Description
Nature,390,249,1997 Science,277,1453,1997
枯草菌168株から抽出したゲノムDNAを鋳型とし、表1に示したyvyD FW1とyvyD/Sp R、及びyvyD/Sp FとyvyD RVの各プライマーセットを用いて、ゲノム上のyvyD遺伝子の上流に隣接する1.0kbp断片(A)、及び下流に隣接する1.0kbp断片(B)をそれぞれ調製した。また、スペクチノマイシン耐性遺伝子を有するプラスミドpDG1727[Gene, 167, 335, (1995)]を鋳型とし、表1に示したSp F及びSp Rのプライマーセットを用いて、スペクチノマイシン耐性遺伝子領域(C)を調製した。次に、得られた(A)(B)(C)3断片を混合して鋳型とし、yvyD FW2とyvyD RV2のプライマーを用いてPCRを行ない、3断片を(A)-(C)-(B)の順になる様に結合させ、遺伝子欠失用のDNA断片を得た(図1参照)。このDNA断片を用いて、コンピテント法により枯草菌168株の形質転換を行い、スペクチノマイシン(0.5μg/mL)を含むLB寒天培地上に生育したコロニーを形質転換体として分離した。さらに得られた形質転換体からゲノムDNAを抽出し、これを鋳型とするPCRによってyvyD遺伝子がスペクチノマイシン耐性遺伝子と置換され、結果として目的とする遺伝子欠失株となっていることを確認した。以上のようにしてyvyD欠失株(168ΔyvyD)を作製した。
実施例1にて得られた168ΔyvyD株、及び対照として枯草菌168株に、Bacillus属細菌 KSM-S237株(FERM BP-7875)由来のアルカリセルラーゼ遺伝子(特開2000-210081号公報)をコードするDNA断片(3.1kb)が、シャトルベクターpHY300PLKのBamHI制限酵素切断点に挿入された組換えプラスミドpHY-S237をプロトプラスト形質転換法によって導入した。これによって得られた菌株を5mLのLB培地で30℃で15時間振盪培養を行い、更にこの培養液0.6mLを30mLの2xL−マルトース培地(2%トリプトン、1%酵母エキス、1%塩化ナトリウム、7.5%マルトース、7.5ppm硫酸マンガン4-5水和物、15ppmテトラサイクリン)に接種し、30℃で3日間、振盪培養を行った(この際、測定誤差を算出する目的で培養を3回行っている)。培養後、遠心分離によって菌体を除いた培養液上清のアルカリセルラーゼ活性を測定し、菌体外に分泌生産されたアルカリセルラーゼの量を求めた。セルラーゼ活性測定については、1/7.5M リン酸緩衝液(pH7.4 和光純薬)で適宜希釈したサンプル溶液50μLに0.4mM p-nitrophenyl-β-D-cellotrioside(生化学工業)を50μL加えて混和し、30℃にて反応を行った際に遊離するp-ニトロフェノール量を420nmにおける吸光度(OD420nm)変化により定量した。1分間に1μmolのp-ニトロフェノールを遊離させる酵素量を1Uとし、生産性比較は、野生株である枯草菌168株の生産性を100%とする相対値により比較した(表2)。この結果、宿主として168ΔyvyD株を用いた場合、対照の168株(野生型)の場合と比較して高いアルカリセルラーゼの分泌生産が認められた。
Claims (8)
- 枯草菌遺伝子yvyD又は当該遺伝子に相当する遺伝子が欠失又は不活性化された微生物株に、異種のタンパク質又はポリペプチドをコードする遺伝子を導入した組換え微生物。
- 枯草菌遺伝子yvyDに相当する遺伝子が、配列番号1に示す塩基配列と80%以上の同一性を有する塩基配列からなる遺伝子である請求項1記載の組み換え微生物。
- 微生物が枯草菌又はその他のバチルス属細菌である請求項1又は2記載の組換え微生物。
- 異種のタンパク質又はポリペプチドをコードする遺伝子の上流に転写開始制御領域、翻訳開始制御領域及び分泌シグナル領域から選ばれる1以上の領域を結合した請求項1〜3のいずれか1項記載の組換え微生物。
- 転写開始制御領域、翻訳開始制御領域及び分泌シグナル領域からなる3領域を結合したものである請求項4記載の組換え微生物。
- 分泌シグナル領域がバチルス属細菌のセルラーゼ遺伝子由来のものであり、転写開始制御領域及び翻訳開始制御領域が当該セルラーゼ遺伝子の上流0.6〜1kb領域由来のものである請求項4又は5記載の組換え微生物。
- 転写開始制御領域、翻訳開始制御領域及び分泌シグナル領域からなる3領域が、配列番号2で示される塩基配列からなるセルラーゼ遺伝子の塩基番号1〜659の塩基配列又は当該塩基配列のいずれかと70%以上の同一性を有する塩基配列からなるDNA断片、又は当該塩基配列の一部が欠失した塩基配列からなるDNA断片である請求項5記載の組換え微生物。
- 請求項1〜7のいずれか1項記載の組換え微生物を用いるタンパク質又はポリペプチドの製造方法。
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Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2012135214A (ja) * | 2010-12-24 | 2012-07-19 | Kao Corp | 組換え微生物を用いたタンパク質の製造方法 |
JP2013017408A (ja) * | 2011-07-08 | 2013-01-31 | National Research Inst Of Brewing | 微生物のコウジ酸生産性を向上させる方法 |
JP2014158428A (ja) * | 2013-02-19 | 2014-09-04 | Kao Corp | アルカリプロテアーゼの生産方法 |
JP2014158429A (ja) * | 2013-02-19 | 2014-09-04 | Kao Corp | ポリ−ガンマ−グルタミン酸の生産方法 |
JP2014158430A (ja) * | 2013-02-19 | 2014-09-04 | Kao Corp | セルラーゼの生産方法 |
WO2023137264A1 (en) | 2022-01-13 | 2023-07-20 | Danisco Us Inc. | Compositions and methods for enhanced protein production in gram‑positive bacterial cells |
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JP2007130013A (ja) * | 2005-10-13 | 2007-05-31 | Kao Corp | 新規枯草菌変異株 |
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JP2007130013A (ja) * | 2005-10-13 | 2007-05-31 | Kao Corp | 新規枯草菌変異株 |
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Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2012135214A (ja) * | 2010-12-24 | 2012-07-19 | Kao Corp | 組換え微生物を用いたタンパク質の製造方法 |
JP2013017408A (ja) * | 2011-07-08 | 2013-01-31 | National Research Inst Of Brewing | 微生物のコウジ酸生産性を向上させる方法 |
JP2014158428A (ja) * | 2013-02-19 | 2014-09-04 | Kao Corp | アルカリプロテアーゼの生産方法 |
JP2014158429A (ja) * | 2013-02-19 | 2014-09-04 | Kao Corp | ポリ−ガンマ−グルタミン酸の生産方法 |
JP2014158430A (ja) * | 2013-02-19 | 2014-09-04 | Kao Corp | セルラーゼの生産方法 |
WO2023137264A1 (en) | 2022-01-13 | 2023-07-20 | Danisco Us Inc. | Compositions and methods for enhanced protein production in gram‑positive bacterial cells |
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