JP2008545394A5 - - Google Patents
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- JP2008545394A5 JP2008545394A5 JP2008512475A JP2008512475A JP2008545394A5 JP 2008545394 A5 JP2008545394 A5 JP 2008545394A5 JP 2008512475 A JP2008512475 A JP 2008512475A JP 2008512475 A JP2008512475 A JP 2008512475A JP 2008545394 A5 JP2008545394 A5 JP 2008545394A5
- Authority
- JP
- Japan
- Prior art keywords
- molecule
- nucleic acid
- chimeric
- seq
- detector
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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- 150000007523 nucleic acids Chemical class 0.000 claims description 106
- 238000000034 method Methods 0.000 claims description 102
- 102000039446 nucleic acids Human genes 0.000 claims description 96
- 108020004707 nucleic acids Proteins 0.000 claims description 96
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 69
- 108091092236 Chimeric RNA Proteins 0.000 claims description 55
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 claims description 50
- 108091093037 Peptide nucleic acid Proteins 0.000 claims description 50
- 210000004027 cell Anatomy 0.000 claims description 42
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims description 40
- 229920001184 polypeptide Polymers 0.000 claims description 38
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 38
- 230000000295 complement effect Effects 0.000 claims description 35
- 102000004190 Enzymes Human genes 0.000 claims description 32
- 108090000790 Enzymes Proteins 0.000 claims description 32
- 230000011664 signaling Effects 0.000 claims description 26
- 108020004414 DNA Proteins 0.000 claims description 24
- 210000004708 ribosome subunit Anatomy 0.000 claims description 22
- 102000053602 DNA Human genes 0.000 claims description 18
- 238000013518 transcription Methods 0.000 claims description 11
- 230000035897 transcription Effects 0.000 claims description 11
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims description 10
- 235000013930 proline Nutrition 0.000 claims description 8
- 150000003148 prolines Chemical class 0.000 claims description 6
- 108020004999 messenger RNA Proteins 0.000 claims description 4
- 238000012163 sequencing technique Methods 0.000 claims description 4
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 claims description 2
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 claims description 2
- 230000001105 regulatory effect Effects 0.000 claims description 2
- 125000003275 alpha amino acid group Chemical group 0.000 claims 4
- 238000001514 detection method Methods 0.000 claims 3
- 239000002773 nucleotide Substances 0.000 description 16
- 125000003729 nucleotide group Chemical group 0.000 description 16
- 108010051109 Cell-Penetrating Peptides Proteins 0.000 description 15
- 102000020313 Cell-Penetrating Peptides Human genes 0.000 description 15
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 15
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 15
- 108091028043 Nucleic acid sequence Proteins 0.000 description 10
- 238000002866 fluorescence resonance energy transfer Methods 0.000 description 10
- WGTODYJZXSJIAG-UHFFFAOYSA-N tetramethylrhodamine chloride Chemical compound [Cl-].C=12C=CC(N(C)C)=CC2=[O+]C2=CC(N(C)C)=CC=C2C=1C1=CC=CC=C1C(O)=O WGTODYJZXSJIAG-UHFFFAOYSA-N 0.000 description 10
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 9
- 241001465754 Metazoa Species 0.000 description 8
- 108010076504 Protein Sorting Signals Proteins 0.000 description 8
- RAHZWNYVWXNFOC-UHFFFAOYSA-N Sulphur dioxide Chemical compound O=S=O RAHZWNYVWXNFOC-UHFFFAOYSA-N 0.000 description 8
- 239000012634 fragment Substances 0.000 description 8
- 108010052853 pVEC peptide Proteins 0.000 description 8
- 108010043655 penetratin Proteins 0.000 description 8
- PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 description 8
- 108010062760 transportan Proteins 0.000 description 8
- 102000009572 RNA Polymerase II Human genes 0.000 description 7
- 102000000395 SH3 domains Human genes 0.000 description 7
- 108050008861 SH3 domains Proteins 0.000 description 7
- 101710137500 T7 RNA polymerase Proteins 0.000 description 7
- 210000003527 eukaryotic cell Anatomy 0.000 description 6
- 102000006271 p21-Activated Kinases Human genes 0.000 description 6
- 108010058266 p21-Activated Kinases Proteins 0.000 description 6
- 150000001413 amino acids Chemical group 0.000 description 5
- 241000124008 Mammalia Species 0.000 description 4
- 239000012472 biological sample Substances 0.000 description 4
- 108010070675 Glutathione transferase Proteins 0.000 description 2
- 102000005720 Glutathione transferase Human genes 0.000 description 2
- 108010043121 Green Fluorescent Proteins Proteins 0.000 description 2
- 102000004144 Green Fluorescent Proteins Human genes 0.000 description 2
- 101710154606 Hemagglutinin Proteins 0.000 description 2
- 101710175625 Maltose/maltodextrin-binding periplasmic protein Proteins 0.000 description 2
- 101710093908 Outer capsid protein VP4 Proteins 0.000 description 2
- 101710135467 Outer capsid protein sigma-1 Proteins 0.000 description 2
- 108091000080 Phosphotransferase Proteins 0.000 description 2
- 101710176177 Protein A56 Proteins 0.000 description 2
- 239000005090 green fluorescent protein Substances 0.000 description 2
- 239000000185 hemagglutinin Substances 0.000 description 2
- 102000020233 phosphotransferase Human genes 0.000 description 2
- 210000001236 prokaryotic cell Anatomy 0.000 description 2
- 241000712461 unidentified influenza virus Species 0.000 description 2
- 239000002299 complementary DNA Substances 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 230000019491 signal transduction Effects 0.000 description 1
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US68233405P | 2005-05-18 | 2005-05-18 | |
| PCT/US2006/019107 WO2006125012A2 (en) | 2005-05-18 | 2006-05-17 | Compositions, methods and kits for real-time nucleic acid analysis in live cells |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2008545394A JP2008545394A (ja) | 2008-12-18 |
| JP2008545394A5 true JP2008545394A5 (cg-RX-API-DMAC7.html) | 2009-07-02 |
Family
ID=37432106
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2008512475A Pending JP2008545394A (ja) | 2005-05-18 | 2006-05-17 | 生存細胞中でのリアルタイム核酸分析のための組成物、方法およびキット |
Country Status (6)
| Country | Link |
|---|---|
| US (2) | US9260703B2 (cg-RX-API-DMAC7.html) |
| EP (1) | EP1882047A4 (cg-RX-API-DMAC7.html) |
| JP (1) | JP2008545394A (cg-RX-API-DMAC7.html) |
| AU (1) | AU2006247215B2 (cg-RX-API-DMAC7.html) |
| CA (1) | CA2609150A1 (cg-RX-API-DMAC7.html) |
| WO (1) | WO2006125012A2 (cg-RX-API-DMAC7.html) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB0804495D0 (en) * | 2008-03-11 | 2008-04-16 | Iti Scotland Ltd | Surface attachment |
| WO2010129019A2 (en) | 2009-04-27 | 2010-11-11 | Pacific Biosciences Of California, Inc. | Real-time sequencing methods and systems |
| WO2010144150A2 (en) | 2009-06-12 | 2010-12-16 | Pacific Biosciences Of California, Inc. | Real-time analytical methods and systems |
| JP7588391B2 (ja) | 2017-06-30 | 2024-11-22 | コデクシス， インコーポレイテッド | Ｔ７ ｒｎａポリメラーゼバリアント |
| CN111417724A (zh) | 2017-06-30 | 2020-07-14 | 科德克希思公司 | T7 rna聚合酶变体 |
| US20230062612A1 (en) * | 2020-01-17 | 2023-03-02 | Sana Biotechnology, Inc. | Safety switches for regulation of gene expression |
| WO2022084748A1 (en) * | 2020-10-23 | 2022-04-28 | Spindle Biotech, Inc. | Compositions and methods for rna synthesis |
| WO2025038950A1 (en) * | 2023-08-16 | 2025-02-20 | Spotlight Therapeutics | Cell-penetrating peptide compositions and uses thereof |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0714980A1 (en) * | 1994-12-02 | 1996-06-05 | Institut Pasteur | Hypermutagenesis |
| US6322901B1 (en) | 1997-11-13 | 2001-11-27 | Massachusetts Institute Of Technology | Highly luminescent color-selective nano-crystalline materials |
| US5990479A (en) | 1997-11-25 | 1999-11-23 | Regents Of The University Of California | Organo Luminescent semiconductor nanocrystal probes for biological applications and process for making and using such probes |
| US6251303B1 (en) | 1998-09-18 | 2001-06-26 | Massachusetts Institute Of Technology | Water-soluble fluorescent nanocrystals |
| US6426513B1 (en) | 1998-09-18 | 2002-07-30 | Massachusetts Institute Of Technology | Water-soluble thiol-capped nanocrystals |
| KR20020086508A (ko) * | 2000-02-08 | 2002-11-18 | 상가모 바이오사이언스 인코포레이티드 | 약물 발견용 세포 |
| US20020025317A1 (en) * | 2000-07-20 | 2002-02-28 | Schering Ag | Bispecific monoclonal antibodies to IL-12 and IL-18 |
| US6576291B2 (en) | 2000-12-08 | 2003-06-10 | Massachusetts Institute Of Technology | Preparation of nanocrystallites |
| US20030165434A1 (en) * | 2001-04-20 | 2003-09-04 | Chiron Corporation | Delivery of polynucleotide agents to the central nervous system |
| JP2003061684A (ja) * | 2001-06-11 | 2003-03-04 | Nippon Genetech Co Ltd | Ｄｎａの塩基配列決定法 |
| JP2005504275A (ja) * | 2001-09-18 | 2005-02-10 | ユー．エス． ジェノミクス， インコーポレイテッド | 高分解能線形解析用のポリマーの差示的タグ付け |
| WO2004007692A2 (en) * | 2002-07-17 | 2004-01-22 | U.S.Genomics, Inc. | Methods and compositions for analyzing polymers using chimeric tags |
| US7343249B2 (en) * | 2005-03-23 | 2008-03-11 | International Business Machines Corporational | Vectorization of sequence alignment computation using distance matrix reshaping |
-
2006
- 2006-05-17 AU AU2006247215A patent/AU2006247215B2/en active Active
- 2006-05-17 CA CA002609150A patent/CA2609150A1/en not_active Abandoned
- 2006-05-17 US US11/920,618 patent/US9260703B2/en active Active
- 2006-05-17 EP EP06770507A patent/EP1882047A4/en not_active Withdrawn
- 2006-05-17 JP JP2008512475A patent/JP2008545394A/ja active Pending
- 2006-05-17 WO PCT/US2006/019107 patent/WO2006125012A2/en not_active Ceased
-
2015
- 2015-12-31 US US14/985,826 patent/US10093917B2/en active Active
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