JP2008541761A5 - - Google Patents
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- Publication number
- JP2008541761A5 JP2008541761A5 JP2008514787A JP2008514787A JP2008541761A5 JP 2008541761 A5 JP2008541761 A5 JP 2008541761A5 JP 2008514787 A JP2008514787 A JP 2008514787A JP 2008514787 A JP2008514787 A JP 2008514787A JP 2008541761 A5 JP2008541761 A5 JP 2008541761A5
- Authority
- JP
- Japan
- Prior art keywords
- minutes
- warming
- paraffin
- aqueous phase
- incubation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 claims 90
- 239000012188 paraffin wax Substances 0.000 claims 24
- 238000010792 warming Methods 0.000 claims 23
- 239000008346 aqueous phase Substances 0.000 claims 22
- 238000011534 incubation Methods 0.000 claims 20
- 238000000746 purification Methods 0.000 claims 15
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 claims 10
- 108091005804 Peptidases Proteins 0.000 claims 10
- 239000004365 Protease Substances 0.000 claims 10
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims 10
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims 10
- 239000002563 ionic surfactant Substances 0.000 claims 10
- 235000019419 proteases Nutrition 0.000 claims 10
- FSYKKLYZXJSNPZ-UHFFFAOYSA-N sarcosine Chemical compound C[NH2+]CC([O-])=O FSYKKLYZXJSNPZ-UHFFFAOYSA-N 0.000 claims 10
- 239000012071 phase Substances 0.000 claims 9
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical group [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 claims 8
- 229920002477 rna polymer Polymers 0.000 claims 6
- 239000004094 surface-active agent Substances 0.000 claims 6
- 108010067770 Endopeptidase K Proteins 0.000 claims 5
- 108010077895 Sarcosine Proteins 0.000 claims 5
- 238000005571 anion exchange chromatography Methods 0.000 claims 5
- YQOKLYTXVFAUCW-UHFFFAOYSA-N guanidine;isothiocyanic acid Chemical compound N=C=S.NC(N)=N YQOKLYTXVFAUCW-UHFFFAOYSA-N 0.000 claims 5
- 238000007899 nucleic acid hybridization Methods 0.000 claims 5
- 238000001556 precipitation Methods 0.000 claims 5
- 229940043230 sarcosine Drugs 0.000 claims 5
- 239000000377 silicon dioxide Substances 0.000 claims 5
- 102000053602 DNA Human genes 0.000 claims 4
- 108020004414 DNA Proteins 0.000 claims 4
- 238000010438 heat treatment Methods 0.000 claims 4
- 239000002736 nonionic surfactant Substances 0.000 claims 4
- IDOQDZANRZQBTP-UHFFFAOYSA-N 2-[2-(2,4,4-trimethylpentan-2-yl)phenoxy]ethanol Chemical group CC(C)(C)CC(C)(C)C1=CC=CC=C1OCCO IDOQDZANRZQBTP-UHFFFAOYSA-N 0.000 claims 2
- 229920001213 Polysorbate 20 Polymers 0.000 claims 2
- 229920004929 Triton X-114 Polymers 0.000 claims 2
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 claims 2
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 claims 2
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US68652205P | 2005-05-31 | 2005-05-31 | |
| US77302706P | 2006-02-13 | 2006-02-13 | |
| PCT/US2006/021011 WO2006130632A2 (en) | 2005-05-31 | 2006-05-31 | Separation and purification of nucleic acid from paraffin-containing samples |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2008541761A JP2008541761A (ja) | 2008-11-27 |
| JP2008541761A5 true JP2008541761A5 (https=) | 2010-09-24 |
Family
ID=37482243
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2008514787A Pending JP2008541761A (ja) | 2005-05-31 | 2006-05-31 | パラフィン含有試料からの核酸の分離及び精製 |
Country Status (4)
| Country | Link |
|---|---|
| US (3) | US9243241B2 (https=) |
| EP (1) | EP1910389A4 (https=) |
| JP (1) | JP2008541761A (https=) |
| WO (1) | WO2006130632A2 (https=) |
Families Citing this family (28)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006130632A2 (en) | 2005-05-31 | 2006-12-07 | Invitrogen Corporation | Separation and purification of nucleic acid from paraffin-containing samples |
| JP2008043332A (ja) * | 2006-08-17 | 2008-02-28 | Panomics Inc | 組織スライドからの核酸定量 |
| EP2262893B1 (en) * | 2008-04-15 | 2013-04-24 | Universität Bern | Method for optimized isolation of rna from fixed tissue |
| DE102008029356A1 (de) * | 2008-06-20 | 2009-12-24 | Siemens Healthcare Diagnostics Gmbh | Verfahren zur Aufreinigung von Nukleinsäuren, insbesondere aus fixiertem Gewebe |
| DE202009018981U1 (de) | 2009-07-01 | 2015-03-11 | Axagarius Gmbh & Co. Kg | Verwendung eines Kits für die Isolierung von Biomolekülen aus biologischen Proben sowie Kit dafür |
| BR112012004699B1 (pt) * | 2009-09-03 | 2019-10-15 | Becton, Dickinson And Company | Métodos, composições e kits para lise química direta |
| CN102822340A (zh) | 2010-04-08 | 2012-12-12 | 恰根有限公司 | 分离和纯化核酸的色谱设备和方法 |
| CN102812118B (zh) | 2010-04-08 | 2016-01-20 | 恰根有限公司 | 分离和纯化核酸的方法 |
| EP2395082A1 (en) * | 2010-06-14 | 2011-12-14 | QIAGEN GmbH | Extraction of nucleic acids from wax-embedded samples |
| US20120077198A1 (en) * | 2010-07-30 | 2012-03-29 | Ambergen, Inc | Compositions And Methods For Cancer Testing |
| TWI425977B (zh) | 2010-11-24 | 2014-02-11 | Ind Tech Res Inst | 將福馬林固定石蠟包埋之組織進行脫蠟的方法與其套組 |
| WO2013083260A1 (en) * | 2011-12-06 | 2013-06-13 | Qiagen Gmbh | Recovery of a biomolecule comprising aqueous phase from a multiphasic mixture |
| EP2730653A1 (en) * | 2012-11-07 | 2014-05-14 | QIAGEN GmbH | Method for lysing a fixed biological sample |
| US20160017314A1 (en) * | 2013-06-06 | 2016-01-21 | Rbc Bioscience Corp. | Method for isolating nucleic acids from formalin-fixed paraffin embedded tissue samples |
| TWI462930B (zh) * | 2013-06-06 | 2014-12-01 | Rbc Bioscience Corp | 福馬林固定石蠟包埋樣本核酸分離方法 |
| JP6201587B2 (ja) * | 2013-09-30 | 2017-09-27 | 凸版印刷株式会社 | 包埋組織からの核酸抽出方法、包埋剤除去用具、及び核酸抽出用キット |
| CA2962782A1 (en) * | 2014-09-26 | 2016-03-31 | The Regents Of The University Of California | Methods and systems for detection of a genetic mutation |
| WO2016161023A1 (en) * | 2015-03-30 | 2016-10-06 | President And Fellows Of Harvard College | Methods to isolate high quality rna from fixed samples |
| JP6909227B2 (ja) * | 2016-02-25 | 2021-07-28 | マサチューセッツ インスチチュート オブ テクノロジー | 臨床組織標本を膨張させるための方法 |
| WO2018119295A1 (en) | 2016-12-22 | 2018-06-28 | Ventana Medical Systems, Inc. | Fully automated nucleic acid extraction method for tissue samples |
| WO2018157074A1 (en) | 2017-02-24 | 2018-08-30 | Massachusetts Institute Of Technology | Methods for diagnosing neoplastic lesions |
| US11873374B2 (en) | 2018-02-06 | 2024-01-16 | Massachusetts Institute Of Technology | Swellable and structurally homogenous hydrogels and methods of use thereof |
| WO2020013833A1 (en) | 2018-07-13 | 2020-01-16 | Massachusetts Institute Of Technology | Dimethylacrylamide (dmaa) hydrogel for expansion microscopy (exm) |
| US12405193B2 (en) | 2019-02-22 | 2025-09-02 | Massachusetts Institute Of Technology | Iterative direct expansion microscopy |
| US12265004B2 (en) | 2019-11-05 | 2025-04-01 | Massachusetts Institute Of Technology | Membrane probes for expansion microscopy |
| CN115335519A (zh) * | 2020-03-31 | 2022-11-11 | 凯杰有限公司 | 从固定的生物样品纯化核酸 |
| CN113322303A (zh) * | 2021-05-12 | 2021-08-31 | 南京中科拜尔医学技术有限公司 | 一种石蜡切片样本核酸提取液及提取方法 |
| JP2025500916A (ja) * | 2021-12-16 | 2025-01-15 | ファウンデーション・メディシン・インコーポレイテッド | 包埋剤を包埋試料から除去する方法 |
Family Cites Families (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5092004A (en) * | 1988-10-11 | 1992-03-03 | Cosco, Inc, | Convertible infant restraint device |
| US5789156A (en) * | 1993-06-14 | 1998-08-04 | Basf Ag | Tetracycline-regulated transcriptional inhibitors |
| CA2153215A1 (en) * | 1994-07-06 | 1996-01-07 | Lu Wang | Treatment of paraffin embedded tissue for gene analysis |
| US6090606A (en) * | 1996-01-24 | 2000-07-18 | Third Wave Technologies, Inc. | Cleavage agents |
| US6914137B2 (en) * | 1997-12-06 | 2005-07-05 | Dna Research Innovations Limited | Isolation of nucleic acids |
| US5912129A (en) * | 1998-03-05 | 1999-06-15 | Vinayagamoorthy; Thuraiayah | Multi-zone polymerase/ligase chain reaction |
| CA2270023C (en) * | 1998-04-28 | 2010-10-12 | Ortho-Clinical Diagnostics, Inc. | Improved methods for extracting nucleic acids from tissue samples and paraffin-embedded tissues |
| US6183998B1 (en) * | 1998-05-29 | 2001-02-06 | Qiagen Gmbh Max-Volmer-Strasse 4 | Method for reversible modification of thermostable enzymes |
| US6544798B1 (en) * | 1999-02-26 | 2003-04-08 | Ventana Medical Systems, Inc. | Removal of embedding media from biological samples and cell conditioning on automated staining instruments |
| US6469159B1 (en) * | 1999-04-26 | 2002-10-22 | Robert T. Belly | Methods for extracting nucleic acids from tissue samples and paraffin-embedded tissues |
| EP1243659B1 (en) * | 1999-12-16 | 2006-04-12 | Katayanagi Institute | Method for detecting target nucleotide sequences |
| EP1130950B1 (en) * | 2000-02-29 | 2007-11-21 | Kyocera Corporation | Wiring board |
| US7001724B1 (en) * | 2000-11-28 | 2006-02-21 | Applera Corporation | Compositions, methods, and kits for isolating nucleic acids using surfactants and proteases |
| GB2369822A (en) | 2000-12-05 | 2002-06-12 | Genovar Diagnostics Ltd | Nucleic acid extraction method and kit |
| CA2518569C (en) | 2003-03-10 | 2011-11-15 | Expression Pathology, Inc. | Liquid tissue preparation from histopathologically processed biological samples, tissues and cells |
| US20050059024A1 (en) | 2003-07-25 | 2005-03-17 | Ambion, Inc. | Methods and compositions for isolating small RNA molecules |
| WO2006130632A2 (en) | 2005-05-31 | 2006-12-07 | Invitrogen Corporation | Separation and purification of nucleic acid from paraffin-containing samples |
-
2006
- 2006-05-31 WO PCT/US2006/021011 patent/WO2006130632A2/en not_active Ceased
- 2006-05-31 US US11/444,171 patent/US9243241B2/en active Active
- 2006-05-31 JP JP2008514787A patent/JP2008541761A/ja active Pending
- 2006-05-31 EP EP06771657A patent/EP1910389A4/en not_active Withdrawn
- 2006-10-23 US US11/552,063 patent/US20070128634A1/en not_active Abandoned
-
2016
- 2016-01-21 US US15/003,179 patent/US20160215281A1/en not_active Abandoned
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