JP2008525517A - ポリペプチドの三硫化物誘導体の形成を防止する方法 - Google Patents
ポリペプチドの三硫化物誘導体の形成を防止する方法 Download PDFInfo
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- JP2008525517A JP2008525517A JP2007548807A JP2007548807A JP2008525517A JP 2008525517 A JP2008525517 A JP 2008525517A JP 2007548807 A JP2007548807 A JP 2007548807A JP 2007548807 A JP2007548807 A JP 2007548807A JP 2008525517 A JP2008525517 A JP 2008525517A
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- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
- C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
- C07K1/1136—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by reversible modification of the secondary, tertiary or quarternary structure, e.g. using denaturating or stabilising agents
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- A61P5/00—Drugs for disorders of the endocrine system
- A61P5/06—Drugs for disorders of the endocrine system of the anterior pituitary hormones, e.g. TSH, ACTH, FSH, LH, PRL, GH
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- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/61—Growth hormone [GH], i.e. somatotropin
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Abstract
【選択図】 なし
Description
hGHの他の多くの効果又は適用が知られている。
関心のあるポリペプチドは、天然又は合成起源であってよく、本発明の方法は、従って、組換えポリペプチド又は制御された条件下で他の方法によって生産されたポリペプチドへの適用に限定されないが、潜在的に、任意の起源、例えば、植物又は動物から抽出されたポリペプチドに適用されることができる。
本願の文脈において、用語「ストリッピング」は、適切なガス(下記参照)による、適切な速度及び温度で、及び適切な持続時間での、液体培地のエアレーションによる、揮発性成分(例えば、環境条件下でその自然な状態がガスである、溶解した成分)の、該液体培地からの置換又は除去を指す。
ガス(ストリッピングガス)及び液体培地の間の接触時間;
ストリッピングガスの液体培地中への導入/通過の速度;
用いるストリッピングガスの容量;
気相と液相の接触表面積の大きさ;
撹拌/混合の活発さ;
液体培地のpH;及び
液体培地及び/又はストリッピングガスの温度。
本発明の方法における液体培地のストリッピングに用いられるガスは、原理的には、関心のあるポリペプチド(又は液体培地中に含まれる他の中心的又は必須の成分)と著しく反応しないか、又は有害に影響しない任意のガスであってよい;従って、例えば、空気、酸素、窒素、ヘリウム、アルゴン、二酸化炭素又はそれらの組合せのようなガスが、本発明に従って潜在的に用いられ得る。しかしながら、液体培地中のH2Sの存在が、ポリペプチドTS誘導体の形成に関して中心的に重要であるという、本発明者らの確信(上記参照)が正しい場合、それに関連する使用に最も適切なそれらのガスは、実質的に化学的に不活性であり、特に、酸化、還元又は他の反応を引き起こすことに関して、及び/又は液体培地に適するpH条件に影響することに関して不活性であり、並びに、実質的に純粋な形態で、容認できるコストで容易に入手可能であるガスであると思われる。これに関して特に関連するガスは、窒素(N2)及びより大量の、容易に入手可能ないわゆる「貴ガス」[ヘリウム(He)、ネオン(Ne)、アルゴン(Ar)、クリプトン(Kr)及びキセノン(Xe)を含む]のメンバー、特にヘリウム及びアルゴン、とりわけアルゴンを含む。それらのガス(特にN2、He及びAr)は容易に、種々の容量のシリンダー中の圧縮形態で、高純度状態で(典型的には、N2について≧99.8 容量%、及びHe及びAr について≧99.9容量%以上) 商業的に入手可能である。
本発明の方法によって得られたか又は得られる、TS誘導体を実質的に含まないポリペプチド(特に、TS-hGH誘導体を実質的に含まない組換えヒト成長ホルモン(rhGH));
薬学的に許容される担体又は希釈剤と共に、そのようなポリペプチドを含む(特に、そのようなrhGHを含む)薬学的組成物;
ヒト成長ホルモンの投与に反応する状態を治療する方法であって、本発明の実質的にTS-hGHを含まないrhGHの治療的有効量を、そのような状態を有する被験者に投与することを含む方法;及び
本発明の実質的にTS-hGHを含まないrhGHの、ヒト成長ホルモンの投与に反応する状態の治療のための薬剤の製造における使用。
本発明の薬学的組成物に関して、適切な担体又は希釈剤の例は、当該分野の通常の技術者には周知である。
発酵ブロス、組換え微生物[組換えヒト成長ホルモン(rhGH)をコードする遺伝子を含む発現カセットを含有する大腸菌]を含むサンプルを、hGH生産バッチから取り出した。該サンプルをホモジナイズし、6つのサブサンプル、それぞれ1500 mlを、2000 mlの測定シリンダーに入れ、磁力によって撹拌した。サブサンプルからのH2Sのストリッピングを、窒素ガス(例えば、エア・リキッド デンマークA/S、Ballerup, Denmarkから入手可能;純度≧99.8%)を、各サブサンプル(温度20℃)に、該測定シリンダーの底に伸ばされたシリコンチューブを介して15-20 l/hourの流速で通気することによって行った。サブサンプル1は、ストリッピングなし(コントロール)、サブサンプル2はストリッピングに2.5時間供され、一方、サブサンプル3、4、5及び6は、ストリッピングに20時間供された。
微細濾過されたサブサンプル中のhGHの量を、hGH配向ELISA方法を用いて測定した:この方法においては、ヒト成長ホルモンに対して作られたモルモットのポリクローナル抗体がマイクロテストプレートに結合される。この抗体(コーティング抗体)は、該サンプルからの成長ホルモンと反応する。hGHに対して作られた、ペルオキシダーゼ標識されたモルモットのポリクローナル抗体(検出抗体)は、次いで、固定された成長ホルモンと反応する。酵素基質(ペルオキシダーゼ基質)の添加の際、ペルオキシダーゼ標識された抗体におけるペルオキシダーゼ活性は、該サンプル中の成長ホルモンの濃度と比例する強度で色の発達をもたらす。該反応は、酸の添加によって停止され、その吸光度が光度計で、450 nmで、並びに620 nmの参照波長で読まれる。
TS-hGHのレベルを、疎水性相互作用クロマトグラフィー(HIC)によって測定した:最適化された測定条件は、サンプル中のhGH濃度がおよそ0.71 g l-1で得られることが発見され、サンプルはそれ故、それらをこの特定のhGH濃度に調節するために希釈された。初期のhGH濃度は、下式に従って277 nmでの吸光度測定から決定される:
chGH =吸光度 (277 nm) × 0.82 (g l-1で)。
A:1M (NH4)2SO4;0,1M Na2HPO4;pH 6.5
B:0,1M Na2HPO4;5% v/v CH3CN;pH 6.5
による勾配溶出を、以下の表にまとめたように行った:
温度、調整されたサンプル:2℃〜8℃
温度、カラムヒーター:28℃±2℃
波長、UV検出:220 nm。
液体サンプル中の硫化水素濃度を、MERCK SpectroquantTM クイックテストを用いて測定した。この試験は、光度分析を用いて、水性サンプル中に溶解している全ての硫化物種を、0.03〜3.3 ppmに及ぶ範囲の濃度で測定するよう設計される。試験キットは、色反応を起こす3つの異なる試薬を含む:硫化水素は、N,N’-ジメチル-1,4-フェニレンジアンモニウムジクロリド(試薬2)と反応して、無色のロイコメチレンブルーを与え、これは次いで、硫酸第二鉄(試薬3)によって酸化されてメチレンブルーになる。スルファミド酸(試薬1)は、亜硝酸塩からの干渉を防止する。メチレンブルーを産生する色反応は、665 nmで最大の明白な吸収をもたらし、ブランクはこの波長でほとんど吸収を示さない。未濾過の発酵ブロスにおけるH2Sレベルの測定のために、3つの試薬を添加した後ブロスを遠心分離した(10分、5000 rpm)。既知の濃度のH2S標準溶液を較正のために用いた。
発酵ブロス、組換え微生物[組換えヒト成長ホルモン(rhGH)をコードする遺伝子を含む発現カセットを含む大腸菌]を含むサンプルを、hGH生産バッチから取り出し、ホモジナイズした。5つの同じサブサンプルを、ホモジナイズされたバルクサンプルから取り出した。それらのサブサンプルの一つ(サブサンプル1)は、ストリッピングに供さなかった;残り4つのサブサンプルは、下記表2に詳述した条件を用いて、窒素ストリッピング[窒素通気された(泡立たせた)、撹拌された発酵槽容器(B. Braun BiostatTM CT;正常作動容量1リットル)において、制御されたpH及び温度で]に供した。実験2及び3は、pHを制御せずに行い、表2に与えられたpH値は初期値である。
Claims (16)
- ポリペプチドを含む液体培地中における該ポリペプチドの三硫化物誘導体の形成を減少するか又は実質的に防止するための方法であって、該方法は、前記液体培地をガスでストリッピングすることを含む方法。
- 前記ポリペプチドが、一以上のジスルフィド結合を含む、請求項1に記載の方法。
- 前記ポリペプチドが、組換え的に生産されたポリペプチドである、請求項1又は2に記載の方法。
- 前記ポリペプチドが、組換え的に生産されたhGH (rhGH);それらの切断型;それらの類似体;及びそれらの誘導体から選択される、請求項3に記載の方法。
- 前記ストリッピングが、前記ポリペプチドの生産の間又は生産後の処理の間に行われる、請求項3又は4に記載の方法。
- 前記ストリッピングが、前記ポリペプチドの生産後の処理の間に行われる、請求項3又は4に記載の方法。
- 前記ガスが化学的に、実質的に不活性なガスである、請求項1〜6の何れか一項に記載の方法。
- 前記ガスが、窒素(N2)、ヘリウム(He)及びアルゴン(Ar)から成る群から選択される、請求項1〜6の何れか一項に記載の方法。
- 前記ポリペプチドが、ストリッピング手順の後に一以上の精製工程に供される、請求項1〜8の何れか一項に記載の方法。
- 少なくとも一つの濾過工程を含む、請求項9に記載の方法。
- 前記ポリペプチドの生産後に前記液体培地に亜硫酸塩又はメルカプト化合物が添加される、請求項5〜10の何れか一項に記載の方法。
- 請求項1〜11の何れか一項に記載の方法によって得られる、TS誘導体を実質的に含まないポリペプチド。
- 請求項1〜11の何れか一項に記載の方法によって得られる、TS-hGH誘導体を実質的に含まない組換えヒト成長ホルモン(rhGH)。
- 請求項12に記載のポリペプチド又は請求項13に記載のrhGHを、薬学的に許容される担体又は希釈剤と共に含む薬学的組成物。
- ヒト成長ホルモンの投与に反応する状態の治療方法であって、請求項13に記載の実質的にTS-hGHを含まないrhGHの治療的有効量を、そのような状態の被験者に投与することを含む方法。
- ヒト成長ホルモンの投与に反応する状態の治療のための薬剤の製造における、請求項13に記載の実質的にTS-hGHを含まないrhGHの使用。
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JP2017526652A (ja) * | 2014-07-24 | 2017-09-14 | ジェネンテック, インコーポレイテッド | 薬剤の少なくとも1つのトリスルフィド結合を含むタンパク質中のチオール部分へのコンジュゲーション方法 |
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US9005926B2 (en) | 2009-10-02 | 2015-04-14 | Biogen Idec Ma Inc. | Methods of preventing and removing trisulfide bonds |
WO2012158551A1 (en) | 2011-05-13 | 2012-11-22 | Biogen Idec Ma Inc. | Methods of preventing and removing trisulfide bonds |
JP7088912B2 (ja) | 2016-09-15 | 2022-06-21 | シティ・オブ・ホープ | ジチオetp誘導体 |
US11230560B2 (en) | 2017-05-22 | 2022-01-25 | City Of Hope | Synthesis of ETP derivatives |
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NL97339C (ja) | 1956-03-02 | |||
JPH0720980B2 (ja) | 1991-11-26 | 1995-03-08 | 株式会社島津製作所 | ペプチド合成装置 |
JPH06220084A (ja) | 1993-01-23 | 1994-08-09 | Shimadzu Corp | ペプチド合成装置 |
DK44593D0 (da) | 1993-04-20 | 1993-04-20 | Novo Nordisk As | Fremgangsmaade til fremstilling af et polypeptid |
ZA955789B (en) * | 1994-07-15 | 1996-03-11 | Novo Nordisk As | A method of converting a hydrophobic derivative of a polypeptide into the native form |
US6299776B1 (en) * | 1997-12-23 | 2001-10-09 | General Signal Corporation | Biochemical oxidation system and process |
SE9802454D0 (sv) * | 1998-07-08 | 1998-07-08 | Pharmacia & Upjohn Ab | Production of peptides |
US20040048315A1 (en) * | 2002-08-28 | 2004-03-11 | Pharmacia Corporation | Method for the preparation of growth hormone and antagonist thereof having lower levels of isoform impurities thereof |
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- 2005-12-20 WO PCT/EP2005/056976 patent/WO2006069940A1/en active Application Filing
- 2005-12-20 AT AT05826671T patent/ATE463503T1/de not_active IP Right Cessation
- 2005-12-20 EP EP05826671A patent/EP1833841B1/en not_active Not-in-force
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Cited By (2)
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JP2017526652A (ja) * | 2014-07-24 | 2017-09-14 | ジェネンテック, インコーポレイテッド | 薬剤の少なくとも1つのトリスルフィド結合を含むタンパク質中のチオール部分へのコンジュゲーション方法 |
US11370838B2 (en) | 2014-07-24 | 2022-06-28 | Genentech, Inc. | Methods of conjugating an agent to a thiol moiety in a protein that contains at least one sulfide bond |
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WO2006069940A1 (en) | 2006-07-06 |
US8530191B2 (en) | 2013-09-10 |
US20100160236A1 (en) | 2010-06-24 |
ES2344120T3 (es) | 2010-08-18 |
DE602005020505D1 (de) | 2010-05-20 |
EP1833841B1 (en) | 2010-04-07 |
EP1833841A1 (en) | 2007-09-19 |
JP4865728B2 (ja) | 2012-02-01 |
US20090131311A1 (en) | 2009-05-21 |
ATE463503T1 (de) | 2010-04-15 |
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