JP2008523376A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2008523376A5 JP2008523376A5 JP2007544985A JP2007544985A JP2008523376A5 JP 2008523376 A5 JP2008523376 A5 JP 2008523376A5 JP 2007544985 A JP2007544985 A JP 2007544985A JP 2007544985 A JP2007544985 A JP 2007544985A JP 2008523376 A5 JP2008523376 A5 JP 2008523376A5
- Authority
- JP
- Japan
- Prior art keywords
- image
- contrast
- imaging
- well
- biological
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000003384 imaging method Methods 0.000 claims description 73
- 238000000034 method Methods 0.000 claims description 48
- 239000002872 contrast media Substances 0.000 claims description 35
- 230000005855 radiation Effects 0.000 claims description 35
- 230000002708 enhancing effect Effects 0.000 claims description 28
- 238000001454 recorded image Methods 0.000 claims description 14
- 239000011248 coating agent Substances 0.000 claims description 12
- 238000000576 coating method Methods 0.000 claims description 12
- 238000001514 detection method Methods 0.000 claims description 6
- 230000005540 biological transmission Effects 0.000 claims description 5
- 231100000252 nontoxic Toxicity 0.000 claims description 5
- 230000003000 nontoxic effect Effects 0.000 claims description 5
- 239000007850 fluorescent dye Substances 0.000 claims description 4
- 108091006047 fluorescent proteins Proteins 0.000 claims description 3
- 102000034287 fluorescent proteins Human genes 0.000 claims description 3
- 239000012216 imaging agent Substances 0.000 claims 7
- 239000012620 biological material Substances 0.000 claims 2
- 238000002834 transmittance Methods 0.000 claims 2
- 210000004027 cell Anatomy 0.000 description 75
- 210000003855 cell nucleus Anatomy 0.000 description 25
- 239000000523 sample Substances 0.000 description 24
- 238000003556 assay Methods 0.000 description 14
- 238000005286 illumination Methods 0.000 description 13
- 239000000126 substance Substances 0.000 description 12
- 150000001875 compounds Chemical class 0.000 description 11
- 238000012545 processing Methods 0.000 description 11
- 238000004113 cell culture Methods 0.000 description 9
- 210000000805 cytoplasm Anatomy 0.000 description 9
- 238000010586 diagram Methods 0.000 description 7
- 239000000463 material Substances 0.000 description 7
- 239000000975 dye Substances 0.000 description 6
- 230000003287 optical effect Effects 0.000 description 6
- 238000000423 cell based assay Methods 0.000 description 5
- 230000005670 electromagnetic radiation Effects 0.000 description 5
- 230000011218 segmentation Effects 0.000 description 5
- 108010043121 Green Fluorescent Proteins Proteins 0.000 description 4
- 102000004144 Green Fluorescent Proteins Human genes 0.000 description 4
- 239000005090 green fluorescent protein Substances 0.000 description 4
- 230000036541 health Effects 0.000 description 4
- 210000004940 nucleus Anatomy 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- 238000004166 bioassay Methods 0.000 description 3
- 238000010191 image analysis Methods 0.000 description 3
- 239000003550 marker Substances 0.000 description 3
- 150000007523 nucleic acids Chemical class 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- PRDFBSVERLRRMY-UHFFFAOYSA-N 2'-(4-ethoxyphenyl)-5-(4-methylpiperazin-1-yl)-2,5'-bibenzimidazole Chemical compound C1=CC(OCC)=CC=C1C1=NC2=CC=C(C=3NC4=CC(=CC=C4N=3)N3CCN(C)CC3)C=C2N1 PRDFBSVERLRRMY-UHFFFAOYSA-N 0.000 description 2
- 108010039918 Polylysine Proteins 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 230000000903 blocking effect Effects 0.000 description 2
- 239000003086 colorant Substances 0.000 description 2
- 238000004040 coloring Methods 0.000 description 2
- 238000004624 confocal microscopy Methods 0.000 description 2
- 230000001086 cytosolic effect Effects 0.000 description 2
- 238000013500 data storage Methods 0.000 description 2
- 210000002288 golgi apparatus Anatomy 0.000 description 2
- 102000039446 nucleic acids Human genes 0.000 description 2
- 108020004707 nucleic acids Proteins 0.000 description 2
- 229920000656 polylysine Polymers 0.000 description 2
- 230000001376 precipitating effect Effects 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 238000000638 solvent extraction Methods 0.000 description 2
- 238000001228 spectrum Methods 0.000 description 2
- 231100000331 toxic Toxicity 0.000 description 2
- 230000002588 toxic effect Effects 0.000 description 2
- 239000013598 vector Substances 0.000 description 2
- 241000579895 Chlorostilbon Species 0.000 description 1
- 102000008186 Collagen Human genes 0.000 description 1
- 108010035532 Collagen Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000016359 Fibronectins Human genes 0.000 description 1
- 108010067306 Fibronectins Proteins 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- 239000013584 assay control Substances 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 238000012984 biological imaging Methods 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 239000012876 carrier material Substances 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 230000036755 cellular response Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 210000004978 chinese hamster ovary cell Anatomy 0.000 description 1
- 229920001436 collagen Polymers 0.000 description 1
- 229920000547 conjugated polymer Polymers 0.000 description 1
- 230000021615 conjugation Effects 0.000 description 1
- 229940039231 contrast media Drugs 0.000 description 1
- 150000004696 coordination complex Chemical class 0.000 description 1
- 210000000172 cytosol Anatomy 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000010339 dilation Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 229940000406 drug candidate Drugs 0.000 description 1
- 238000007876 drug discovery Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 229910052876 emerald Inorganic materials 0.000 description 1
- 239000010976 emerald Substances 0.000 description 1
- 108010048367 enhanced green fluorescent protein Proteins 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000003628 erosive effect Effects 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- 238000000799 fluorescence microscopy Methods 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 238000003709 image segmentation Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 238000009830 intercalation Methods 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 210000003463 organelle Anatomy 0.000 description 1
- 239000002831 pharmacologic agent Substances 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 229920002714 polyornithine Polymers 0.000 description 1
- 108010055896 polyornithine Proteins 0.000 description 1
- 238000012805 post-processing Methods 0.000 description 1
- 238000007781 pre-processing Methods 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 230000012743 protein tagging Effects 0.000 description 1
- 230000007398 protein translocation Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 238000007873 sieving Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 231100000167 toxic agent Toxicity 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 230000005945 translocation Effects 0.000 description 1
- 241001515965 unidentified phage Species 0.000 description 1
- 238000012800 visualization Methods 0.000 description 1
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB0427050.0A GB0427050D0 (en) | 2004-12-10 | 2004-12-10 | Method of,and apparatus and computer software for,imaging biological objects |
| PCT/GB2005/004747 WO2006061640A2 (en) | 2004-12-10 | 2005-12-09 | Method of, and apparatus and computer software for imaging biological objects |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2008523376A JP2008523376A (ja) | 2008-07-03 |
| JP2008523376A5 true JP2008523376A5 (https=) | 2012-07-26 |
Family
ID=34073478
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2007544985A Pending JP2008523376A (ja) | 2004-12-10 | 2005-12-09 | 生体の画像化の方法、ならびにそのための装置およびコンピュータソフトウェア |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US7881509B2 (https=) |
| EP (1) | EP1831668A2 (https=) |
| JP (1) | JP2008523376A (https=) |
| GB (1) | GB0427050D0 (https=) |
| WO (1) | WO2006061640A2 (https=) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9329127B2 (en) | 2011-04-28 | 2016-05-03 | Bio-Rad Laboratories, Inc. | Fluorescence scanning head with multiband detection |
| EP2749868B1 (en) * | 2011-08-26 | 2019-02-27 | Olympus Corporation | Single-particle detector using optical analysis, single-particle detection method using same, and computer program for single-particle detection |
| EP2840380A4 (en) | 2012-04-18 | 2015-11-25 | Olympus Corp | SINGLE PARTICLE DETECTOR WITH PHOTO ANALYSIS, SINGLE PARTICLE PROCESSING AND COMPUTER PROGRAM FOR SINGLE PARTICLE DETECTION |
| WO2013157283A1 (ja) * | 2012-04-18 | 2013-10-24 | オリンパス株式会社 | 標的粒子の検出方法 |
| GB2528864A (en) * | 2014-07-31 | 2016-02-10 | Technion Res & Dev Foundation | Spectral imaging using single-axis spectrally dispersed illumination |
| JP2020515828A (ja) * | 2017-04-07 | 2020-05-28 | ヴェリリー ライフ サイエンシズ エルエルシー | 落射蛍光集光用のパターン形成光学系 |
| CN110892251B (zh) | 2017-07-11 | 2023-09-15 | 浜松光子学株式会社 | 试样观察装置和试样观察方法 |
| JP7298993B2 (ja) * | 2018-04-09 | 2023-06-27 | 浜松ホトニクス株式会社 | 試料観察装置及び試料観察方法 |
| US12117380B2 (en) | 2018-11-15 | 2024-10-15 | University Of Houston System | Milling with ultraviolet excitation |
| US12578272B2 (en) | 2020-12-02 | 2026-03-17 | Agilent Technologies, Inc. | Universal multi-detection system for microplates with confocal imaging |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1998038490A1 (en) * | 1997-02-27 | 1998-09-03 | Cellomics, Inc. | A system for cell-based screening |
| US6416959B1 (en) * | 1997-02-27 | 2002-07-09 | Kenneth Giuliano | System for cell-based screening |
| US5958675A (en) * | 1997-04-18 | 1999-09-28 | 3M Innovative Properties Company | Method for detecting bacteria using bacteriophage, contrast-coloring dye and precipitable dye |
| US6241662B1 (en) * | 1997-10-20 | 2001-06-05 | Lifespex, Inc. | Acetic acid as a signal enhancing contrast agent in fluorescence spectroscopy |
| CA2324262C (en) * | 1998-03-16 | 2010-05-25 | Praelux Incorporated | Confocal microscopy imaging system |
| US6388788B1 (en) * | 1998-03-16 | 2002-05-14 | Praelux, Inc. | Method and apparatus for screening chemical compounds |
| US20030036855A1 (en) * | 1998-03-16 | 2003-02-20 | Praelux Incorporated, A Corporation Of New Jersey | Method and apparatus for screening chemical compounds |
| GB9920758D0 (en) | 1999-09-03 | 1999-11-03 | Nycomed Amersham Plc | Improved container composition for diagnostic agents |
| US6633368B2 (en) * | 2001-01-02 | 2003-10-14 | Becton, Dickinson And Company | Method for determining the volume of single red blood cells |
-
2004
- 2004-12-10 GB GBGB0427050.0A patent/GB0427050D0/en not_active Ceased
-
2005
- 2005-12-09 US US11/721,185 patent/US7881509B2/en not_active Expired - Fee Related
- 2005-12-09 WO PCT/GB2005/004747 patent/WO2006061640A2/en not_active Ceased
- 2005-12-09 EP EP05820416A patent/EP1831668A2/en not_active Withdrawn
- 2005-12-09 JP JP2007544985A patent/JP2008523376A/ja active Pending
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP4532261B2 (ja) | 生物学的試料のための光線画像分析 | |
| Kukulski et al. | Precise, correlated fluorescence microscopy and electron tomography of lowicryl sections using fluorescent fiducial markers | |
| US9329130B2 (en) | Systems and methods for counting cells and biomolecules | |
| JP5540102B2 (ja) | 高められた病理学的決定のための複数モダリティコントラストおよび明視野コンテキスト表現、および組織内の複数検体検出 | |
| JP4812937B2 (ja) | 共焦点顕微鏡イメージングシステム | |
| JP5338573B2 (ja) | 蛍光像取得方法、蛍光像取得装置及び蛍光像取得プログラム | |
| US7408176B2 (en) | System and method employing photokinetic techniques in cell biology imaging applications | |
| CN103718044B (zh) | 使用基于细胞的免疫荧光法使用合成校正粒子自动测定免疫荧光灶点的方法和系统 | |
| JP5112694B2 (ja) | 生体物質の分析 | |
| JP2002355090A (ja) | 細胞に基づくスクリーニングシステム | |
| JP2005525550A (ja) | 細胞の迅速自動化スクリーニングシステム及び方法 | |
| JP2002526788A (ja) | ハイスループット顕微鏡 | |
| Gough et al. | Requirements, features, and performance of high content screening platforms | |
| CN116802463A (zh) | 具有共焦成像的用于微孔板的通用多功能检测系统 | |
| US7881509B2 (en) | Method of, and apparatus and computer software for, imaging biological objects | |
| JP2008523376A5 (https=) | ||
| JP5482057B2 (ja) | 細胞核を構成する構造体の解析方法 | |
| Denner et al. | High-content analysis in preclinical drug discovery | |
| Lemercier et al. | Microtome-integrated microscope system for high sensitivity tracking of in-resin fluorescence in blocks and ultrathin sections for correlative microscopy | |
| Daly et al. | 3D super-resolution imaging of PSD95 reveals an abundance of diffuse protein supercomplexes in the mouse brain | |
| CA2544706A1 (fr) | Analyse automatique d'echantillons cellulaires | |
| JP5362666B2 (ja) | 細胞観察装置および細胞観察方法 | |
| JP4990791B2 (ja) | 動的試料を分析するための方法および装置 | |
| US11067785B2 (en) | Method for generating fluorescent photograph using fluorescence microscope | |
| US20200056995A1 (en) | Biological substance analysis method, biological substance analyzer, biological substance analysis program, and biological substance analysis system |