WO2006061640A2 - Method of, and apparatus and computer software for imaging biological objects - Google Patents

Method of, and apparatus and computer software for imaging biological objects Download PDF

Info

Publication number
WO2006061640A2
WO2006061640A2 PCT/GB2005/004747 GB2005004747W WO2006061640A2 WO 2006061640 A2 WO2006061640 A2 WO 2006061640A2 GB 2005004747 W GB2005004747 W GB 2005004747W WO 2006061640 A2 WO2006061640 A2 WO 2006061640A2
Authority
WO
WIPO (PCT)
Prior art keywords
image
biological objects
enhancing agent
contrast
contrast enhancing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/GB2005/004747
Other languages
English (en)
French (fr)
Other versions
WO2006061640A3 (en
Inventor
Elaine J. Adie
Nicholas Solvatore Arini
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
GE Healthcare UK Ltd
Original Assignee
GE Healthcare UK Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by GE Healthcare UK Ltd filed Critical GE Healthcare UK Ltd
Priority to JP2007544985A priority Critical patent/JP2008523376A/ja
Priority to EP05820416A priority patent/EP1831668A2/en
Priority to US11/721,185 priority patent/US7881509B2/en
Publication of WO2006061640A2 publication Critical patent/WO2006061640A2/en
Publication of WO2006061640A3 publication Critical patent/WO2006061640A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1429—Signal processing
    • G01N15/1433—Signal processing using image recognition
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/10—Segmentation; Edge detection
    • G06T7/11—Region-based segmentation
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
    • G06V20/00—Scenes; Scene-specific elements
    • G06V20/60—Type of objects
    • G06V20/69—Microscopic objects, e.g. biological cells or cellular parts
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/10—Image acquisition modality
    • G06T2207/10056—Microscopic image
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/10—Image acquisition modality
    • G06T2207/10064—Fluorescence image
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/30—Subject of image; Context of image processing
    • G06T2207/30004—Biomedical image processing
    • G06T2207/30024—Cell structures in vitro; Tissue sections in vitro

Definitions

  • a method of imaging one or more biological objects using imaging apparatus capable of capturing an image across an imaging area, said method including: a) placing said one or more biological objects in an environment; b) providing in said environment, outside of said one or more biological objects, a contrast enhancing agent which provides contrast in an image between said one or more biological objects and said environment; and c) recording an image of said one or more biological objects and said environment using said imaging apparatus, whereby a spatial definition for said one or more biological objects is derivable using contrast in said image which is provided by said contrast enhancing agent.
  • the invention also provides, in a further aspect, apparatus arranged to perform the above method.
  • multi-well plate 180 which contains a two-dimensional array of sample wells 182.
  • the optical elements of Figure 2 are depicted in cross-section and the multi-well plate in perspective.
  • the projection of the line of illumination onto multi-well plate 180 is depicted by line 184 and is also understood to be perpendicular to the plane of Figure 2.
  • multi-well plate 180 may be moved in two dimensions (X, Y) parallel to the dimensions of the array by means not shown.
  • the cells 236 are distributed in a material located outside of the cells 236 which contains a predetermined concentration of a contrast enhancing agent (CEA) which is distributed approximately homogeneously throughout the material.
  • CEA contrast enhancing agent
  • the material is a medium 237 which is non-toxic to the cells 236, which supports the cells 236 by providing nutrients and conditions which maintain a health of the cells 236 and which helps the cell culture to grow.
  • the cells 236 form a layer across the base plate 234 which has a thickness of approximately one cell, hi further embodiments, the layer may have a thickness which is greater than one cell.
  • the imaging system additionally records, in addition to the image recorded in accordance with previous embodiments, a second image 260 of the cells and the environment across the imaging area in a second channel of the imaging system.
  • the second CEA provides a contrast between relatively light areas 262 of the second image which corresponds to cell nuclei of the cells and relatively dark areas 264 corresponding to areas surrounding the cell nuclei which include the cell cytoplasm and the medium.
  • These two recorded images are co-registered such that the pixels of each image are aligned.
  • This is saved in a file known as an image stack.
  • An image stack is a collection of images that logically belong together for analysis purposes. In most cases, a given image stack will contain images that share a common acquisition.
  • the segmented object areas are next assigned S3 as cells, and regions outside of these segmented areas are assigned as the medium.
  • relatively dark object areas of the image are separated out and assigned as the cells.
  • relatively light object areas of the thresholded image are separated out and assigned as the cells.

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Molecular Biology (AREA)
  • Theoretical Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Multimedia (AREA)
  • Signal Processing (AREA)
  • Dispersion Chemistry (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Apparatus For Radiation Diagnosis (AREA)
PCT/GB2005/004747 2004-12-10 2005-12-09 Method of, and apparatus and computer software for imaging biological objects Ceased WO2006061640A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
JP2007544985A JP2008523376A (ja) 2004-12-10 2005-12-09 生体の画像化の方法、ならびにそのための装置およびコンピュータソフトウェア
EP05820416A EP1831668A2 (en) 2004-12-10 2005-12-09 Method of, and apparatus and computer software for imaging biological objects
US11/721,185 US7881509B2 (en) 2004-12-10 2005-12-09 Method of, and apparatus and computer software for, imaging biological objects

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0427050.0 2004-12-10
GBGB0427050.0A GB0427050D0 (en) 2004-12-10 2004-12-10 Method of,and apparatus and computer software for,imaging biological objects

Publications (2)

Publication Number Publication Date
WO2006061640A2 true WO2006061640A2 (en) 2006-06-15
WO2006061640A3 WO2006061640A3 (en) 2007-02-22

Family

ID=34073478

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2005/004747 Ceased WO2006061640A2 (en) 2004-12-10 2005-12-09 Method of, and apparatus and computer software for imaging biological objects

Country Status (5)

Country Link
US (1) US7881509B2 (https=)
EP (1) EP1831668A2 (https=)
JP (1) JP2008523376A (https=)
GB (1) GB0427050D0 (https=)
WO (1) WO2006061640A2 (https=)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9329127B2 (en) 2011-04-28 2016-05-03 Bio-Rad Laboratories, Inc. Fluorescence scanning head with multiband detection
EP3654020A4 (en) * 2017-07-11 2021-04-07 Hamamatsu Photonics K.K. SAMPLE MONITORING DEVICE AND SAMPLE MONITORING METHOD
US12117380B2 (en) 2018-11-15 2024-10-15 University Of Houston System Milling with ultraviolet excitation
EP4256289A4 (en) * 2020-12-02 2024-10-30 Agilent Technologies, Inc. UNIVERSAL MULTIPLE DETECTION SYSTEM FOR MICROPLATES WITH CONFOCAL IMAGING

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2749868B1 (en) * 2011-08-26 2019-02-27 Olympus Corporation Single-particle detector using optical analysis, single-particle detection method using same, and computer program for single-particle detection
EP2840380A4 (en) 2012-04-18 2015-11-25 Olympus Corp SINGLE PARTICLE DETECTOR WITH PHOTO ANALYSIS, SINGLE PARTICLE PROCESSING AND COMPUTER PROGRAM FOR SINGLE PARTICLE DETECTION
WO2013157283A1 (ja) * 2012-04-18 2013-10-24 オリンパス株式会社 標的粒子の検出方法
GB2528864A (en) * 2014-07-31 2016-02-10 Technion Res & Dev Foundation Spectral imaging using single-axis spectrally dispersed illumination
JP2020515828A (ja) * 2017-04-07 2020-05-28 ヴェリリー ライフ サイエンシズ エルエルシー 落射蛍光集光用のパターン形成光学系
JP7298993B2 (ja) * 2018-04-09 2023-06-27 浜松ホトニクス株式会社 試料観察装置及び試料観察方法

Family Cites Families (9)

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WO1998038490A1 (en) * 1997-02-27 1998-09-03 Cellomics, Inc. A system for cell-based screening
US6416959B1 (en) * 1997-02-27 2002-07-09 Kenneth Giuliano System for cell-based screening
US5958675A (en) * 1997-04-18 1999-09-28 3M Innovative Properties Company Method for detecting bacteria using bacteriophage, contrast-coloring dye and precipitable dye
US6241662B1 (en) * 1997-10-20 2001-06-05 Lifespex, Inc. Acetic acid as a signal enhancing contrast agent in fluorescence spectroscopy
CA2324262C (en) * 1998-03-16 2010-05-25 Praelux Incorporated Confocal microscopy imaging system
US6388788B1 (en) * 1998-03-16 2002-05-14 Praelux, Inc. Method and apparatus for screening chemical compounds
US20030036855A1 (en) * 1998-03-16 2003-02-20 Praelux Incorporated, A Corporation Of New Jersey Method and apparatus for screening chemical compounds
GB9920758D0 (en) 1999-09-03 1999-11-03 Nycomed Amersham Plc Improved container composition for diagnostic agents
US6633368B2 (en) * 2001-01-02 2003-10-14 Becton, Dickinson And Company Method for determining the volume of single red blood cells

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9329127B2 (en) 2011-04-28 2016-05-03 Bio-Rad Laboratories, Inc. Fluorescence scanning head with multiband detection
EP3654020A4 (en) * 2017-07-11 2021-04-07 Hamamatsu Photonics K.K. SAMPLE MONITORING DEVICE AND SAMPLE MONITORING METHOD
US11353402B2 (en) 2017-07-11 2022-06-07 Hamamatsu Photonics K.K. Sample observation device and sample observation method
US12031911B2 (en) 2017-07-11 2024-07-09 Hamamatsu Photonics K.K. Sample observation device and sample observation method
US12117380B2 (en) 2018-11-15 2024-10-15 University Of Houston System Milling with ultraviolet excitation
EP4256289A4 (en) * 2020-12-02 2024-10-30 Agilent Technologies, Inc. UNIVERSAL MULTIPLE DETECTION SYSTEM FOR MICROPLATES WITH CONFOCAL IMAGING
US12578272B2 (en) 2020-12-02 2026-03-17 Agilent Technologies, Inc. Universal multi-detection system for microplates with confocal imaging

Also Published As

Publication number Publication date
JP2008523376A (ja) 2008-07-03
US7881509B2 (en) 2011-02-01
GB0427050D0 (en) 2005-01-12
WO2006061640A3 (en) 2007-02-22
US20090232370A1 (en) 2009-09-17
EP1831668A2 (en) 2007-09-12

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