JP2008509682A - Ｙｅｂｆを利用するタンパク質の製造方法 - Google Patents

Ｙｅｂｆを利用するタンパク質の製造方法 Download PDF

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Publication number
JP2008509682A
JP2008509682A JP2007526148A JP2007526148A JP2008509682A JP 2008509682 A JP2008509682 A JP 2008509682A JP 2007526148 A JP2007526148 A JP 2007526148A JP 2007526148 A JP2007526148 A JP 2007526148A JP 2008509682 A JP2008509682 A JP 2008509682A
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Prior art keywords
protein
yebf
expression vector
polypeptide
peptide
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JP2007526148A
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Japanese (ja)
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JP2008509682A5 (enExample
Inventor
ジョエル ワイナー
グイジン ザン
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ガヴァナーズ オブ ザ ユニヴァーシティー オブ アルバータ
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Publication of JP2008509682A publication Critical patent/JP2008509682A/ja
Publication of JP2008509682A5 publication Critical patent/JP2008509682A5/ja
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2007526148A 2004-08-18 2005-08-12 Ｙｅｂｆを利用するタンパク質の製造方法 Pending JP2008509682A (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US52212504P 2004-08-18 2004-08-18
PCT/CA2005/001249 WO2006017929A1 (en) 2004-08-18 2005-08-12 Protein production method utilizing yebf

Publications (2)

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JP2008509682A true JP2008509682A (ja) 2008-04-03
JP2008509682A5 JP2008509682A5 (enExample) 2008-09-18

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JP2007526148A Pending JP2008509682A (ja) 2004-08-18 2005-08-12 Ｙｅｂｆを利用するタンパク質の製造方法

Country Status (7)

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US (1) US20060246539A1 (enExample)
EP (1) EP1791961B1 (enExample)
JP (1) JP2008509682A (enExample)
AT (1) ATE512230T1 (enExample)
AU (1) AU2005274628A1 (enExample)
CA (1) CA2576083A1 (enExample)
WO (1) WO2006017929A1 (enExample)

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2014517835A (ja) * 2011-05-11 2014-07-24 チルドレンズ メディカル センター コーポレーション 修飾ビオチン結合タンパク質、その融合タンパク質、および用途
US11013793B2 (en) 2018-09-12 2021-05-25 Affinivax, Inc. Multivalent pneumococcal vaccines
JP2022520327A (ja) * 2019-02-15 2022-03-30 オンコシミス バイオテック プライベート リミテッド タンパク質分泌増強のための細菌発現ベクター
JP2022522276A (ja) * 2019-02-24 2022-04-15 オンコシミス バイオテック プライベート リミテッド 細菌による組換えｇｌｐ－１ペプチドの連続生産方法
US11612647B2 (en) 2017-06-23 2023-03-28 University Of Maryland, Baltimore Immunogenic compositions
US12036276B2 (en) 2021-09-09 2024-07-16 Affinivax, Inc. Multivalent pneumococcal vaccines
US12129283B2 (en) 2018-09-12 2024-10-29 The Children's Medical Center Corporation Pneumococcal fusion protein vaccines

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102006050332A1 (de) 2006-10-25 2008-04-30 Wacker Chemie Ag DNS-Konstrukt und Verfahren zur fermentativen Herstellung von Fusionsproteinen
EP4272750A3 (en) 2013-02-07 2024-01-24 Children's Medical Center, Corp. Protein antigens that provide protection against pneumococcal colonization and/or disease
US9616114B1 (en) 2014-09-18 2017-04-11 David Gordon Bermudes Modified bacteria having improved pharmacokinetics and tumor colonization enhancing antitumor activity
SG11201703528YA (en) * 2014-11-03 2017-05-30 Univ Nanyang Tech A recombinant expression system that senses pathogenic microorganisms
US10676723B2 (en) 2015-05-11 2020-06-09 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
US11365225B2 (en) 2015-11-19 2022-06-21 President And Fellows Of Harvard College Method of making recombinant silk and silk-amyloid hybrid proteins using bacteria
US12351805B2 (en) 2015-11-19 2025-07-08 Northeastern University Method of making recombinant silk and silk-amyloid hybrid proteins using bacteria
US11180535B1 (en) 2016-12-07 2021-11-23 David Gordon Bermudes Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria
CA3061709A1 (en) 2017-03-28 2018-10-04 The Children's Medical Center Corporation A multiple antigen presenting system (maps)-based staphylococcus aureus vaccine, immunogenic composition, and uses thereof
US11471497B1 (en) 2019-03-13 2022-10-18 David Gordon Bermudes Copper chelation therapeutics
US10973908B1 (en) 2020-05-14 2021-04-13 David Gordon Bermudes Expression of SARS-CoV-2 spike protein receptor binding domain in attenuated salmonella as a vaccine

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000066756A1 (en) * 1999-05-04 2000-11-09 Timo Korpela Microbial protein expression system
JP2004521656A (ja) * 2001-08-14 2004-07-22 コリア・アドヴァンスド・インスティテュート・オブ・サイエンス・アンド・テクノロジー ＯｍｐＦを用いて目的タンパク質を菌体外へ生産する方法

Family Cites Families (10)

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CA1335357C (en) * 1987-10-30 1995-04-25 Timothy M. Rose Expression systems for preparation of polypeptides in prokaryotic cells
US5646016A (en) * 1991-02-06 1997-07-08 Genetics Institute, Inc. Peptide and protein fusions to thioredoxin, thioredoxin-like molecules, and modified thioredoxin-like molecules
US6033877A (en) * 1995-11-02 2000-03-07 Research Foundation Of State University Of New York Peptide expression and delivery system
US6022952A (en) * 1998-04-01 2000-02-08 University Of Alberta Compositions and methods for protein secretion
US6733997B1 (en) * 1998-10-30 2004-05-11 National University Of Singapore Isolated nucleic acids encoding a secretory signal for expression and secretion of heterologous recombinant proteins
AU6124201A (en) * 2000-05-03 2001-11-12 Expressive Constructs Inc A method and device for improving protein stability and solubility
AU2002213681A1 (en) * 2000-11-09 2002-05-21 The University Of Queensland Bacterial expression systems
US20040142429A1 (en) * 2001-01-25 2004-07-22 Grant Chris W.M. Method for the expression, purification, and structure recovery and handling of hydrophobic proteins, polypeptides or peptides
DE60238532D1 (de) * 2001-05-04 2011-01-20 Cornell Res Foundation Inc Schnell spaltbares sumofusionsprotein-expressionssystem für schwer zu exprimierende proteine
US6790051B1 (en) * 2003-12-20 2004-09-14 Lear Corporation Connector for printed circuit board

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000066756A1 (en) * 1999-05-04 2000-11-09 Timo Korpela Microbial protein expression system
JP2004521656A (ja) * 2001-08-14 2004-07-22 コリア・アドヴァンスド・インスティテュート・オブ・サイエンス・アンド・テクノロジー ＯｍｐＦを用いて目的タンパク質を菌体外へ生産する方法

Cited By (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2023162283A (ja) * 2011-05-11 2023-11-08 ザ チルドレンズ メディカル センター コーポレーション 修飾ビオチン結合タンパク質、その融合タンパク質、および用途
JP2018197237A (ja) * 2011-05-11 2018-12-13 ザ チルドレンズ メディカル センター コーポレーション 修飾ビオチン結合タンパク質、その融合タンパク質、および用途
US10611805B2 (en) 2011-05-11 2020-04-07 Children's Medical Center Corporation Modified biotin-binding protein, fusion proteins thereof and applications
JP2014517835A (ja) * 2011-05-11 2014-07-24 チルドレンズ メディカル センター コーポレーション 修飾ビオチン結合タンパク質、その融合タンパク質、および用途
US11560410B2 (en) 2011-05-11 2023-01-24 Children's Medical Center Corporation Modified biotin-binding protein, fusion proteins thereof and applications
US11976097B2 (en) 2011-05-11 2024-05-07 Children's Medical Center Corporation Modified biotin-binding protein, fusion proteins thereof and applications
US11981708B2 (en) 2011-05-11 2024-05-14 Children's Medical Center Corporation Multiple antigen presenting immunogenic composition, and methods and uses thereof
JP7679431B2 (ja) 2011-05-11 2025-05-19 ザ チルドレンズ メディカル センター コーポレーション 修飾ビオチン結合タンパク質、その融合タンパク質、および用途
US11612647B2 (en) 2017-06-23 2023-03-28 University Of Maryland, Baltimore Immunogenic compositions
US11013793B2 (en) 2018-09-12 2021-05-25 Affinivax, Inc. Multivalent pneumococcal vaccines
US12370247B2 (en) 2018-09-12 2025-07-29 Affinivax, Inc. Multivalent pneumococcal vaccines
US11701416B2 (en) 2018-09-12 2023-07-18 Affinivax, Inc. Multivalent pneumococcal vaccines
US12129283B2 (en) 2018-09-12 2024-10-29 The Children's Medical Center Corporation Pneumococcal fusion protein vaccines
JP2022520327A (ja) * 2019-02-15 2022-03-30 オンコシミス バイオテック プライベート リミテッド タンパク質分泌増強のための細菌発現ベクター
JP7517707B2 (ja) 2019-02-15 2024-07-17 オンコシミス バイオテック プライベート リミテッド タンパク質分泌増強のための細菌発現ベクター
JP7487951B2 (ja) 2019-02-24 2024-05-21 オンコシミス バイオテック プライベート リミテッド 細菌による組換えｇｌｐ－１ペプチドの連続生産方法
JP2022522276A (ja) * 2019-02-24 2022-04-15 オンコシミス バイオテック プライベート リミテッド 細菌による組換えｇｌｐ－１ペプチドの連続生産方法
US12036276B2 (en) 2021-09-09 2024-07-16 Affinivax, Inc. Multivalent pneumococcal vaccines
US12377140B2 (en) 2021-09-09 2025-08-05 Affinivax, Inc. Multivalent pneumococcal vaccines

Also Published As

Publication number Publication date
ATE512230T1 (de) 2011-06-15
EP1791961A1 (en) 2007-06-06
CA2576083A1 (en) 2006-02-23
AU2005274628A1 (en) 2006-02-23
EP1791961B1 (en) 2011-06-08
US20060246539A1 (en) 2006-11-02
EP1791961A4 (en) 2008-11-19
WO2006017929A1 (en) 2006-02-23

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