CA2576083A1 - Protein production method utilizing yebf - Google Patents

Protein production method utilizing yebf Download PDF

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Publication number
CA2576083A1
CA2576083A1 CA002576083A CA2576083A CA2576083A1 CA 2576083 A1 CA2576083 A1 CA 2576083A1 CA 002576083 A CA002576083 A CA 002576083A CA 2576083 A CA2576083 A CA 2576083A CA 2576083 A1 CA2576083 A1 CA 2576083A1
Authority
CA
Canada
Prior art keywords
protein
yebf
encodes
expression vector
polypeptide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002576083A
Other languages
English (en)
French (fr)
Inventor
Joel Weiner
Guijin Zhang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2576083A1 publication Critical patent/CA2576083A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CA002576083A 2004-08-18 2005-08-12 Protein production method utilizing yebf Abandoned CA2576083A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US52212504P 2004-08-18 2004-08-18
US60/522,125 2004-08-18
PCT/CA2005/001249 WO2006017929A1 (en) 2004-08-18 2005-08-12 Protein production method utilizing yebf

Publications (1)

Publication Number Publication Date
CA2576083A1 true CA2576083A1 (en) 2006-02-23

Family

ID=35907194

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002576083A Abandoned CA2576083A1 (en) 2004-08-18 2005-08-12 Protein production method utilizing yebf

Country Status (7)

Country Link
US (1) US20060246539A1 (enExample)
EP (1) EP1791961B1 (enExample)
JP (1) JP2008509682A (enExample)
AT (1) ATE512230T1 (enExample)
AU (1) AU2005274628A1 (enExample)
CA (1) CA2576083A1 (enExample)
WO (1) WO2006017929A1 (enExample)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102006050332A1 (de) 2006-10-25 2008-04-30 Wacker Chemie Ag DNS-Konstrukt und Verfahren zur fermentativen Herstellung von Fusionsproteinen
CN108714212B (zh) * 2011-05-11 2024-09-06 儿童医疗中心有限公司 多抗原提呈免疫原性组合物及其方法和用途
EP4272750A3 (en) 2013-02-07 2024-01-24 Children's Medical Center, Corp. Protein antigens that provide protection against pneumococcal colonization and/or disease
US9616114B1 (en) 2014-09-18 2017-04-11 David Gordon Bermudes Modified bacteria having improved pharmacokinetics and tumor colonization enhancing antitumor activity
SG11201703528YA (en) * 2014-11-03 2017-05-30 Univ Nanyang Tech A recombinant expression system that senses pathogenic microorganisms
US10676723B2 (en) 2015-05-11 2020-06-09 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
US11365225B2 (en) 2015-11-19 2022-06-21 President And Fellows Of Harvard College Method of making recombinant silk and silk-amyloid hybrid proteins using bacteria
US12351805B2 (en) 2015-11-19 2025-07-08 Northeastern University Method of making recombinant silk and silk-amyloid hybrid proteins using bacteria
US11180535B1 (en) 2016-12-07 2021-11-23 David Gordon Bermudes Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria
CA3061709A1 (en) 2017-03-28 2018-10-04 The Children's Medical Center Corporation A multiple antigen presenting system (maps)-based staphylococcus aureus vaccine, immunogenic composition, and uses thereof
SG11201912601RA (en) 2017-06-23 2020-01-30 Nosocomial Vaccine Corp Immunogenic compositions
JP7535791B2 (ja) 2018-09-12 2024-08-19 ザ チルドレンズ メディカル センター コーポレーション 肺炎球菌融合タンパク質ワクチン
US11013793B2 (en) 2018-09-12 2021-05-25 Affinivax, Inc. Multivalent pneumococcal vaccines
SG11202105519YA (en) * 2019-02-15 2021-06-29 Oncosimis Biotech Private Ltd A bacterial expression vector for enhanced protein secretion
EP3927733A4 (en) * 2019-02-24 2022-07-06 Oncosimis Biotech Private Limited PROCESS FOR THE CONTINUOUS PRODUCTION OF RECOMBINANT GLP-1 PEPTIDE BY BACTERIA
US11471497B1 (en) 2019-03-13 2022-10-18 David Gordon Bermudes Copper chelation therapeutics
US10973908B1 (en) 2020-05-14 2021-04-13 David Gordon Bermudes Expression of SARS-CoV-2 spike protein receptor binding domain in attenuated salmonella as a vaccine
WO2023039223A1 (en) 2021-09-09 2023-03-16 Affinivax, Inc. Multivalent pneumococcal vaccines

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1335357C (en) * 1987-10-30 1995-04-25 Timothy M. Rose Expression systems for preparation of polypeptides in prokaryotic cells
US5646016A (en) * 1991-02-06 1997-07-08 Genetics Institute, Inc. Peptide and protein fusions to thioredoxin, thioredoxin-like molecules, and modified thioredoxin-like molecules
US6033877A (en) * 1995-11-02 2000-03-07 Research Foundation Of State University Of New York Peptide expression and delivery system
US6022952A (en) * 1998-04-01 2000-02-08 University Of Alberta Compositions and methods for protein secretion
US6733997B1 (en) * 1998-10-30 2004-05-11 National University Of Singapore Isolated nucleic acids encoding a secretory signal for expression and secretion of heterologous recombinant proteins
FI109361B (fi) * 1999-05-04 2002-07-15 Korpela Timo Mikrobinen proteiinin ilmentämisjärjestelmä
AU6124201A (en) * 2000-05-03 2001-11-12 Expressive Constructs Inc A method and device for improving protein stability and solubility
AU2002213681A1 (en) * 2000-11-09 2002-05-21 The University Of Queensland Bacterial expression systems
US20040142429A1 (en) * 2001-01-25 2004-07-22 Grant Chris W.M. Method for the expression, purification, and structure recovery and handling of hydrophobic proteins, polypeptides or peptides
DE60238532D1 (de) * 2001-05-04 2011-01-20 Cornell Res Foundation Inc Schnell spaltbares sumofusionsprotein-expressionssystem für schwer zu exprimierende proteine
KR100447530B1 (ko) * 2001-08-14 2004-09-08 한국과학기술원 ＯｍｐＦ를 이용하여 목적 단백질을 대장균 세포외로분비생산하는 방법
US6790051B1 (en) * 2003-12-20 2004-09-14 Lear Corporation Connector for printed circuit board

Also Published As

Publication number Publication date
ATE512230T1 (de) 2011-06-15
JP2008509682A (ja) 2008-04-03
EP1791961A1 (en) 2007-06-06
AU2005274628A1 (en) 2006-02-23
EP1791961B1 (en) 2011-06-08
US20060246539A1 (en) 2006-11-02
EP1791961A4 (en) 2008-11-19
WO2006017929A1 (en) 2006-02-23

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Legal Events

Date Code Title Description
FZDE Discontinued